JOURNAL OF ALLOYS AND COMPOUNDS
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2016
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IF 3.014
Hongji Li,Xiao Wei,Yeqing Xu,Kai Lu,Yufeng Zhang,Yongsheng Yan,Chunxiang Li
This investigation presented a novel strategy for the preparation of hybrid structure silica/ZnO/molecularly imprinted polymers (SiO 2 /ZnO/MIPs) to fluorescence recognization and detection of the pyrethroids. Firstly, carboxylated SiO 2 nanoparticles (Si-NPs) and amino-functionalized ZnO quantum dots (QDs) were linked together by the function of amination under the assistance of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 1-hydroxy-5-pyrrolidinedione (NHS). Next, the “sunny shape” molecular imprinted polymers (MIPs) layers were anchored on the surface of SiO 2 /ZnO QDs by the technique of atom transfer radical polymerization (ATRP) and surface molecular imprinting. The resulting nanoparticles were characterized by TEM , FT-IR, fluorescence spectra and the adsorptive properties were detected by the UV–vis spectrophotometer. Through the results, the strategy shortened the preparation time and presented more rapid and sensitive detection performance than the traditional methods. The fluorescence detection results demonstrated this material owned special recognition property to the templates, which contributed that the fluorescence intensity decreased linearly with the variation of pyrethroids in the concentration range 1.0–80 μmol L −1 under the optimized conditions and the detection limit was 0.13 μmol L −1 . Moreover, the successful applications in practical samples indicated that the synthesis method in this investigation provided a novel way to the pyrethroids detection in river.
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高影响因子
PROCESS BIOCHEMISTRY
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2016
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IF 2.529
Ru Song,Kai-qiang Zhang,Rong-bian Wei
Squid viscera, one of the major by-products in squid processing, were hydrolyzed to generate bioactive autolysates using their endogenous proteases. In vitro antioxidative activities of squid viscera autolysates (SVAs) were evaluated. The SVAs demonstrated strong activity on scavenging 1,1-diphenyl-2-picryl-hydrazil (DPPH) and hydroxyl radicals, as well as reducing power ability. The SVAs were purified using size exclusion chromatography and RP-HPLC. Nineteen peptides were identified in the active fraction SVAs3 by liquid chromatography-electrospray ionization/multi-stage mass spectrometry (LC-ESI-Q-TOF-MS/MS). Among these identified peptides, six peptides with relatively small molecular weights (700–800 Da), LLAPPER, FPGLADR, WVAPLK, FFNPVH, FNVVLK and LELPLK, were synthesized for assaying antioxidative activity in vitro. Peptide WVAPLK demonstrated strong scavenging effects on free radicals, with the IC 50 values of 0.82 ± 0.08 mg/mL (or 1.14 ± 0.11 mM) for DPPH radical and 1.85 ± 0.04 mg/mL (or 2.60 ± 0.06 mM) for hydroxyl radical, respectively. The low molecular weight and hydrophobic residue W at the N-terminus and basic residue K at the C-terminus as well as specific residues P and L within sequence should play a key role for the high antioxidative activity of WVAPLK. The results suggested that the SVAs could be used as a source of antioxidants and peptides.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
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2017
·Vol. 40
·期 22
·4446-4452
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IF 2.557
Wei Wang,Ruiguang Bai,Xiaoyu Cai,Ping Lin,Lihong Ma
A method using high-speed capillary micellar electrokinetic chromatography and a microbial fuel cell was applied to determine the metabolite of the peptides released by Bacillus licheniformis . Two peptides, l -carnosine and l -alanyl- l -glutamine were used as the substrate to feed Bacillus licheniformis in a microbial fuel cell. The metabolism process of the bacterium was monitored by analyzing the voltage outputs of the microbial fuel cell. A home-made spontaneous injection device was applied to perform high-speed capillary micellar electrokinetic chromatography. Under the optimized conditions, tryptophan, glycine, valine, tyrosine and the two peptides could be rapidly separated within 2.5 min with micellar electrokinetic chromatography mode. Then the method was applied to analyze the solutions sampled from the microbial fuel cell. After 92 h running, valine, as the metabolite, was successfully detected with concentration 3.90 × 10 −5 M. The results demonstrated that Bacillus licheniformis could convert l -carnosine and l -alanyl- l -glutamine into valine. The method employed in this work was proved to have great potential in analysis of metabolites, such as amino acids, for microorganisms.
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
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IF 3.981
Chusheng Liu,Haibin Li,Qiqin Wang,Jacques Crommen,Haibo Zhou,Zhengjin Jiang
The quest for higher column efficiency is one of the major research areas in polymer-based monolithic column fabrication. In this research, two novel polymer-based HILIC monolithic columns with 400 μm I.D. × 800 μm O.D. were prepared based on the thermally initiated co-polymerization of N,N -dimethyl- N -(3-methacrylamidopropyl)- N -(3-sulfopropyl) ammonium betaine (SPP) and ethylene glycol dimethacrylate (EDMA) or N,N’ -methylenebisacrylamide (MBA). In order to obtain a satisfactory performance in terms of column permeability, mechanical stability, efficiency and selectivity, the polymerization parameters were systematically optimized. Column efficiencies as high as 142, 000 plates/m and 120, 000 plates/m were observed for the analysis of neutral compounds at 0.6 mm/s on the poly(SPP- co -MBA) and poly(SPP- co -EDMA) monoliths, respectively. Furthermore, the Van Deemter plots for thiourea on the two monoliths were compared with that on a commercial silica based ZIC-HILIC column (3.5 μm, 200 Å, 150 mm × 300 μm I.D.) using ACN/H 2 O (90/10, v/v) as the mobile phase at room temperature. It was noticeable that the Van Deemter curves for both monoliths, particularly the poly(SPP- co -MBA) monolith, are significantly flatter than that obtained for the ZIC-HILIC column, which indicates that in spite of their larger internal diameters, they yield better overall efficiency, with less peak dispersion, across a much wider range of usable linear velocities. A clearly better separation performance was also observed for nucleobases, nucleosides, nucleotides and small peptides on the poly(SPP- co -MBA) monolith compared to the ZIC-HILIC column. It is particularly worth mentioning that these 400 μm I.D. polymer-based HILIC monolithic columns exhibit enhanced mechanical strength owing to the thicker capillary wall of the fused-silica capillaries.
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PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2024
·
IF 5.6
Lu Wang,Limei Xiao,Shoujing Zheng,Jie Pang,Jiebo Chen
A better understanding of amino acids (AA) characteristics is beneficial for breeding, processing and storage of sugarcane. The differences and classification in AA of 10 different sugarcane varieties (S1-S10) were investigated in this study. Varieties affected components and concentrations of free AA ( p ＜ 0.05) significantly. The total AA of sugarcanes ranged from 51.44 to 781.61 mg/L, while the content of essential amino acids (EAA) was in the range of 0.72–60.98 mg/L. Glu (glutamic acid) was the most abundant free amino acids in all varieties of sugarcane except S4. Even though S6 had the highest concentration of medicinal AA (781.61 mg/L), the highest proportion of medicinal AA in total AA was found in S5 (81.76%). S6 got the highest comprehensive score in amino acids based on the results of Principal Component Analysis (PCA). The cluster analysis roughly divided the sugarcanes into 2 groups. Group Ⅰ including S1, S6, S2 and S5 got positive scores in PCA might had great edible and medicinal development and utilization value.
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Matter
·
2025
·
IF 17.5
Shang Chi,Yaping Li,Tingting Ye,Jiawei Kang,Zhihui Xiang,Xiaoqing Kuang,Chenggang Yi,Yiying Qi,Wei Wang
This work developed a de novo sonosensitizer based on carnosine_zinc piezoelectric metal-organic frameworks (PMOFs) that possesses a prominent reactive oxygen species (ROS)-generating function, biocompatibility, and degradability. Further, the PMOF was modified by a carbon monoxide (CO) donor through a metal complexation reaction to result in a multifunctional CO-PMOF to launch CO/ROS-mediated antimicrobial under ultrasound (US). However, the heat generated during sonodynamic therapy (SDT) may harm wounds. To minimize the waste energy and promote wound self-closure rate, a strong adhesive, and rapid thermal-responsive contraction (52.4%, 4 min), injectable hydrogel was designed to load CO-PMOF. The resulting ultrasonic triple-responsive hydrogel (UTGel) exhibits an effective biofilm destruction capability based on a CO/ROS-mediated antimicrobial therapy. After administration in an infected diabetic skin wound model in mice, UTGel can efficiently harvest the thermal energy by the CO-PMOF under US to trigger a centripetal shrinkage of the hydrogel and guide rapid wound self-closure.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Siyu Wu,Xiaojuan Niu,Jiali Chen,Wangni Meng,Han Tao,Jianli Zhou,Yuangen Wu
Kanamycin (KAN) can generate a serious threat to human health due to its tendency to accumulate in food. Current colorimetric strategy for KAN detection still faces some challenges, and nanozyme-based colorimetry is an ideal alternative approach. Herein, a non‑copper-based multivalent laccase mimic named Mn-Car is first synthesized through a self-assembly of manganese ions and the endogenous biomolecule carnosine, which is further used to develop a novel colorimetric method for ultrasensitive and selective detection of KAN. The laccase-like catalytic center is formed through the multidentate chelation bonds between manganese ions and imidazolium groups of histidine as well as carboxyl groups of β-alanine in carnosine. The multivalent oxidation states of manganese dynamically regulate electron transfer pathways through synergistic coordination. The unique Mn-Car architecture drives the efficient generation of superoxide radicals (O 2 · − ) and semiquinone radicals (SQ· − ). Leveraging the excellent laccase-like activity of Mn-Car , a highly sensitive colorimetric method for KAN detection is developed. Mn-Car forms polydentate chelation with the amino and hydroxyl groups in KAN, which can trigger multi-step cascade oxidation reactions that disrupt the structural core of Mn-Car and lead to reduced colorimetric signals. The colorimetric method achieves a limit of detection (LOD) of 0.102 μM and is successfully applied for on-site detection of KAN in water samples and honey, providing a novel platform for antibiotic residue analysis.
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高影响因子
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