BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2025
·
IF 1.3
Yang Zhongyi,Gong Ruhai,Mimata Yoshiharu,Ye Shaosong,Ji Wei,Ye Wenxiu
A key tricarboxylic acid cycle metabolite, malate, accumulates in leaves during dehydration and induces stomatal closure by recruiting cytosolic Ca2+, activating a SLOW ANION CHANNEL-ASSOCIATED 1, and promoting reactive oxygen species. However, the effects of malate on stomatal opening and its underlying molecular mechanisms remain poorly understood. Our study revealed that, among TCA cycle metabolites, malate specifically inhibited light-induced stomatal opening in both grapevine and Arabidopsis. We demonstrated that SLAC1 was required for malate's inhibitory effects. The inhibition by malate was disrupted by Ca2+ signaling inhibitors. Additionally, malate signal was mediated by G-proteins, which regulate the production of second messengers. ROS production was abolished when G-proteins were inhibited. These findings show that malate efficiently maintains stomatal closure by not only inducing stomatal closure but also inhibiting stomatal opening. The inhibition of stomatal opening by malate is mediated through the activation of SLAC1 and the G-protein signaling cascade.
研究领域:
植物生理学
·
分子生物学
·
细胞信号传导
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产品关联
DOI
PubMed
高影响因子
Results in Physics
·
2016
·
IF 1.337
Feiyan Liu,Jiyuan Liang,Chang Zhang,Liang Yu,Jinxing Zhao,Chang Liu,Qian Lan,Shengrui Chen,Yuan-Cheng Cao,Guang Zheng
Reduced graphene oxide (rGO) encapsulated sulfur spheres for the Li-S batteries were prepared via the redox reaction between sodium polysulfide. XRD spectra showed that the diffraction peak of graphite oxide (GO) at 10° disappeared, while the relatively weak diffraction peak at 27° belongs to graphene emerged. FT-IR spectra showed that the vibrations of the functional groups of GO, such as 3603 cm −1 , 1723 cm −1 and 1619 cm −1 which contributed from OH, C O C and C O respectively, disappeared when compared to the spectra of GSC. SEM observations indicated that the optimum experimental condition followed as: mass ratio of GO and S was 1:1, 10% NaOH was used to adjust the pH. EDX analysis showed that the sulfur content reached at 68.8% of the composite material. The resultant electric resistance was nearly less than GO’s resistance in three orders of magnitude under same condition. Further electrochemical performance tests showed a coulombic efficiency was 96% from the first cycle capacity was 827 mAh g −1 , to 388 mAh g −1 in the 100 cycles. This study carries substantial significance to the development of Li-S battery cathode materials.
研究领域:
材料科学
·
能源技术
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产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2021
·Vol. 9
·期 35
·19681-19686
·
IF 12.732
Zhongjie Yang,Caoyu Yang,Jianyu Han,Wenshi Zhao,Shengxian Shao,Siyu Li,Huiwen Gao,Haijiao Xie,Xiaofei Zhang
Selective electrochemical CO2 reduction to formic acid is an appealing strategy to achieve efficient utilization of CO2. Modification of the catalytic interface with organic linkers has been proven to promote the conversion of CO2 to CO, but the highly selective generation of formic acid is still a challenge. Herein, modification of the interface of graphene oxide (GO) with amide linkages to anchor SnO2 nanoparticles is presented to catalyze the CO2 reduction reaction (CO2RR). As expected, the as-synthesized SnO2/tert-GO shows a competitive high cathodic faradaic efficiency (FE) of 84.4% for formate at −0.96 V and shows long-term stability for the CO2RR. Further, density functional theory (DFT) calculations indicated that amide linkages facilitate the conversion of CO2 to formate.
研究领域:
电化学
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材料科学
·
催化
·
环境科学
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产品关联
DOI
高影响因子
Oxidative Medicine and Cellular Longevity
·
2017
·
IF 4.593
Hui Zhou,Baocai Fu,Bo Xu,Xiangquan Mi,Gang Li,Chengjun Ma,Jianxin Xie,Ji Li,Zhenhua Wang
Endothelial dysfunction is the key player in the development and progression of vascular events. Oxidative stress is involved in endothelial injury. Rosmarinic acid (RA) is a natural polyphenol with antioxidative, antiapoptotic, and anti-inflammatory properties. The present study investigates the protective effect of RA on endothelial dysfunction induced by hydrogen peroxide (H2O2). Compared with endothelium-denuded aortic rings, the endothelium significantly alleviated the decrease of vasoconstrictive reactivity to PE and KCl induced by H2O2. H2O2 pretreatment significantly injured the vasodilative reactivity to ACh in endothelium-intact aortic rings in a concentration-dependent manner. RA individual pretreatment had no obvious effect on the vasoconstrictive reaction to PE and KCl, while its cotreatment obviously mitigated the endothelium-dependent relaxation impairments and the oxidative stress induced by H2O2. The RA cotreatment reversed the downregulation of AMPK and eNOS phosphorylation induced by H2O2 in HAEC cells. The pretreatment with the inhibitors of AMPK (compound C) and eNOS (L-NAME) wiped off RA’s beneficial effects. All these results demonstrated that RA attenuated the endothelial dysfunction induced by oxidative stress by activating the AMPK/eNOS pathway.
研究领域:
药理学
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心血管研究
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2016
·
IF 3.42
Yaming Gu,Yizhen Guo,Changyong Wang,Jiake Xu,Jianping Wu,Thomas Brett Kirk,Dong Ma,Wei Xue
It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo . Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.
研究领域:
生物医学工程
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肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2018
·Vol. 36
·期 9
·1070-1076
·
IF 2.016
Zhou Yong,Bai Pei,Huo Miao,Chen Yu-Jie,Li Hua,Kang Hong-Mei,Liu He-Zhou,Guo Yun-Long
Isocyanate-treated graphite oxides ( i GOs) were well-dispersed into the polystyrene (PS) thin films and formed a novel network structure. With control in fabrication, an i GOs-web layer was horizontally embedded near the surface of the films and thus formed a composite slightly doped by i GOs. This work demonstrated that the i GOs network can remarkably depress the dewetting process in the polymer matrix of the composite, while dewetting often leads to rupture of polymer films and is considered as a major practical limit in using polymeric materials above their glass transition temperatures ( T g ). Via annealing the 50–120 nm thick composite and associated neat PS films at temperatures ranging from 35 °C to 70 °C above T g , surface morphology evolution of the films was monitored by atomic force microscopy (AFM). The i GOs-doped PS exhibited excellent thermal stability, i.e. , the number of dewetting holes was greatly reduced and the long-term hole growth was fairly restricted. In contrast, the neat PS film showed serious surface fluctuation and a final rupture induced by ordinary dewetting. The method developed in this work may pave a road to reinforce thin polymer films and enhance their thermal stability, in order to meet requirements by technological advances.
研究领域:
材料科学
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高分子化学
✓ 已验证
产品关联
DOI
高影响因子
MEAT SCIENCE
·
2020
·
IF 3.644
Huiting Luo,Peijun Li,Hongwei Zhang,Xinping Diao,Baohua Kong
The objective of this study was to clarify whether formation of nitrosylmyoglobin (MbFe II NO) by Lactobacillus fermentum AS1.1880 in meat is due to nitric oxide synthase (NOS) activity. Confocal laser scanning microscopy exhibited strong green fluorescence in the L . fermentum sample treated with a nitric oxide (NO)-specific probe, directly indicating that NO was produced. Furthermore, determination of NOS activity based on the presence of NO metabolites indicated the existence of NOS in L.fermentum . A NOS inhibitor, N G -nitro-L-arginine methyl ester, significantly inhibited the activity of NOS in L.fermentum ( P < 0.05). Futhermore, NOS protein was detected in L.fermentum by Western blot analysis. L-arginine addition largely increased the NOS activity of L.fermentum ( P < 0.05). In meat batters , the redness of a sample inoculated with L.fermentum was higher than that of the control and colour was significantly improved with the addition of L-arginine ( P < 0.05), indicating that more MbFe II NO was formed.
研究领域:
食品科学
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微生物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Rui Liu,Yingxue Xu,Wanfen Pu,Peng Shi,Daijun Du,James J. Sheng,Huaisong Yong
Graphene oxide (GO) has continued to prompt fantastic discoveries and applications; however, the highly extended two-dimensional π-conjugation of pristine graphene sheets leads to the aggregation of GO sheets in strong brine, which impairs the applications of GO. In this work GO with covalently-bound oligomeric ethylene–glycol brushes (termed as GOC) was prepared using graphite oxide as a raw material. The synthesized GOC was investigated by a holistic combination of experimental characterizations and molecular dynamic simulations. Our results showed that GOC behaves like a highly anisotropic colloid that well dispersed in various organic solvents, and retained high dispersion stability in strong brine even after storage at high temperature (e.g, 90 ℃). The GOC can sharply decrease surface tension after reaching a very low threshold concentration of 0.04 mg/mL; on account of that, it successfully generated water-in-oil Pickering emulsions with an internal water content of as high as 90 vol%. The GOC also functions as a liquid crystal at the oil–water interface without the need of external assistances/stimuli. We envisage that the prepared multifunctional GOC materials will stimulate a broad range of its applications when exceptional interfacial properties are required such as in the field of food, cosmetics, and petroleum industries.
研究领域:
材料科学
·
界面化学
✓ 已验证
产品关联
DOI
高影响因子
Coatings
·
2023
·Vol. 13
·期 7
·1297
·
IF 3.4
Sihui Wang,Zhengdong Li,Xiaopeng Gong,Feng Hou,Ji Liang
Porous cellulose aerogels with different compositions have been fabricated via three methods, including regular freezing, directional freezing, and hydrothermal treatment, using pure cellulose oxide and cellulose oxide/graphite oxide composites, respectively. The cellulose aerogels are highly elastic and light in weight. The carbon aerogels show an ordered structure through directional freezing with layered skeleton bones and some folds. Unlike low-temperature freezing, the structures can obtain elastic properties. When the deformation is 20%, carbon aerogels can rebound to 95% of their original volume. The cellulose oxide/graphite oxide composite aerogels are synthesized into carbon–aerogel composites, which also have stable and robust structures of bone skeletons due to nanosheets. The carbon–aerogel composites have more than 85% resilience under 40% deformations. Carbon aerogels prepared from nanocelluloses have a novel three-dimensional network structure and have the application of elasticity, which is expected to be applied to metallurgical technology and the aerospace field. Through directional freezing, the carbon aerogels have regular structures of layered skeleton bones with some folds. In contrast to low-temperature freezing, the structures possess excellent elastic properties.Keywords:cellulose aerogel;carbon aerogel;directional freezing;temple free
研究领域:
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2023
·
IF 6.2
Lu Dou,Man Yuan,Daiqi Li,Sizhuo Zou,Sirui Tan,Zhong Zhao,Guangming Cai
Wearable sensors, which feature textile substrates integrated with conductive layers, have received increasing attention from researchers due to their huge potential in health management. However, the inevitable damage of conductive layer caused by friction and bacterial growth on the textile substrate during its direct contact with bare skin are still the issues that hinder the long-term service of wearable sensors. Herein, a core-sheath yarn was prepared by wrapping polyester (PET) fibers coated with reduced graphene oxide (rGO)/silver nanoparticles (Ag NPs) around the spandex filaments via friction spinning. The core-sheath structure guarantees good prevention of the interface composed of rGO and Ag NPs in between from friction, while the introduction of Ag NPs ensures antibacterial activity of the yarn. The morphology and chemical composition of the nanomaterials deposited on the surface of PET fibers was investigated to reveal the sensing mechanism of the yarn. Meanwhile, its sensing performance was comprehensively evaluated from the perspectives of sensitivity, durability and comparison of commercial ECG. Despite the fact that inevitable damage of the rGO/Ag layer occurred during the friction spinning process, the core-sheath yarn still featured a gauge factor of 8.11 and a threshold of sensing range up to 68.57 %. Apart from being able to detect respiratory signals, cardio signals with the frequency of 70 beat per min (bpm) was also recorded. Compared to signals with the frequency of 73 bpm recorded by a commercial ECG sensor simultaneously, the core-sheath yarn exhibited satisfying precision. Moreover, the existence of Ag NPs in the conductive interface endowed the yarn with ability to yield 13-mm and 15-mm wide growth inhibition zones of E. coli and S. aureus , respectively. The cardiorespiratory sensing ability coupled with antibacterial activity of the yarn suggested its satisfying sensitivity and prolonged service time, making it a promising wearable sensor subjected to direct skin contact for long-time cardiorespiratory monitoring.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
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