ACS Applied Bio Materials
·
2020
·
IF 0
Faming Wang,Pengfei Ren,Katrien V. Bernaerts,Yifu Fu,Wanjun Hu,Naizhen Zhou,Tianzhu Zhang
As one of the nonenzymatic cell-harvesting technologies, a thermal-responsive surface based on poly(2-oxazoline)s has achieved initial success in supporting the adhesion and thermal-induced detachment of animal cells. However, because of the laborious preparation procedure, this technique was only limited to research purposes. In this work, through using poly(glycidyl methacrylate) (PGMA) as the anchor layer, poly(2-propyl-2-oxazoline)s (PPOx) were grafted onto glass wafers through a facile two-step coating and annealing procedure for nonenzymatic cell harvesting. In the first step, the piranha solution-activated glass wafers were immersed into the chloroform solution of PGMA and then annealed for a given period of time to immobilize PGMA onto the glass wafers through the bonding between epoxy groups and hydroxyl groups. In the second step, the PGMA-coated glass wafers were further immersed into the chloroform solution of carboxyl-functionalized PPOx. After annealing, PPOx were immobilized onto the PGMA layer through the bonding between carboxyl groups and the residual epoxy groups. Atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry were used to characterize the modified glass wafers. The results of cytocompatibility evaluation showed that the PPOx-coated glass wafers were almost nontoxic and were able to support the adhesion and proliferation of L929 cells well. By lowering the temperature to 8 °C, L929 and Vero cells were successfully detached from the PPOx-coated glass wafers without any enzymatic treatment. Further cultivation has demonstrated that the cooling procedure had little effect on cell viability, and the cells still retained good viability after harvesting.
研究领域:
材料科学
·
生物技术
·
细胞生物学
·
表面化学
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产品关联
DOI
PubMed
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2024
·
IF 2.3
Zhuochen Zhang,Ying Zhang,Yabin Guo,Cheng Qian,Kailun Chen,Sheng Fang,Anna Qiu,Liangjun Zhong,Jian Zhang,Rui He
Electrospinning technology has recently attracted increased attention in the biomedical field, and preparing various cellulose nanofibril membranes for periodontal tissue regeneration has unique advantages. However, the characteristics of using a single material tend to make it challenging to satisfy the requirements for a periodontal barrier film, and the production of composite fibrous membranes frequently impacts the quality of the final fiber membrane due to the influence of miscibility between different materials. In this study, nanofibrous membranes composed of polylactic acid (PLA) and polycaprolactone (PCL) fibers were fabricated using side-by-side electrospinning. Different concentrations of gelatin were added to the fiber membranes to improve their hydrophilic properties. The morphological structure of the different films as well as their composition, wettability and mechanical characteristics were examined. The results show that PCL/PLA dual-fibrous composite membranes with an appropriate amount of gelatin ensures sufficient mechanical strength while obtaining improved hydrophilic properties. The viability of L929 fibroblasts was evaluated using CCK-8 assays, and cell adhesion on the scaffolds was confirmed by scanning electron microscopy and by immunofluorescence assays. The results demonstrated that none of the fibrous membranes were toxic to cells and the addition of gelatin improved cell adhesion to those membranes. Based on our findings, adding 30% gelatin to the membrane may be the most appropriate content for periodontal tissue regeneration, considering the scaffold’s mechanical qualities, hydrophilic properties and biocompatibility. In addition, the PCL-gelatin/PLA-gelatin dual-fibrous membranes prepared using side-by-side electrospinning technology have potential applications for tissue engineering.
研究领域:
生物医学工程
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组织工程
·
材料科学
·
细胞生物学
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2018
·
IF 3.741
Yu Zhang,Pan He,Xinming Liu,Hongyu Zhang,Huailin Yang,Chunsheng Xiao,Xuesi Chen
Synthetic polymers that can change their physicochemical properties in response to physiological stimuli have attracted particular interest for constructing smart drug delivery systems. In the present work, a novel oxidation-responsive block copolymer bearing thioether pendants was designed and synthesized by ring-opening alternating copolymerization (ROAP) of phthalic anhydride (PA) and 2-(methylthio)ethyl glycidyl ether (MTG) monomer using mPEG-OH as the macroinitiator and a phosphazene base, t -BuP 1 , as the catalyst. The obtained mPEG- b -P(PA- alt -MTG) copolymer could self-assemble into spherical micelles in water with an average size of about 30 nm and a critical micelle concentration value of 7.44 × 10 −3 mg/mL. 1 H NMR results indicated that the hydrophobic thioether group can be selectively oxidized to hydrophilic sulfoxide by H 2 O 2 , leading to disassociation of the micelles as illustrated by the TEM observation. Nile Red, as a model drug, was loaded into the copolymer micelles. The intensity of the fluorescence emission decreased sharply after addition of H 2 O 2 , indicating an oxidation-triggered drug release. Moreover, the drug-loaded micelles could be effectively internalized into MCF-7 cells, as indicated by the confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) studies. MTT assay further demonstrated that the synthesized copolymer showed good biocompatibility towards L929 cell, even at a concentration up to 0.5 mg/mL. In sum, this kind of copolymer may have great potential applications in oxidation responsive drug delivery.
研究领域:
材料科学
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药学
·
聚合物化学
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药物递送系统
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生物医学工程
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Ling Zhou,Weihua Zhang,Changwen Zhao,Wantai Yang
Developing coating materials with low cytotoxicity and high antimicrobial activity has been recognized as an effective way to prevent medical device-associated infections. In this study, a maleic anhydride terpolymer (PPTM) is synthesized and covalently attached to silicone rubber (SR) surface. The formed coating can be further cross-linked (SPM) through the self-condensation of pendent siloxane groups of terpolymer. No crack or delamination of SPM was observed after 500 cycles of bending and 7 day immersion in deionized water. The sliding friction force of a catheter was reduced by 50% after coating with SPM. The SPM coating without adding any extra antibacterial reagents can kill 99.99% of Staphylococcus aureus and Escherichia coli and also significantly reduce bacterial coverage, while the coating displayed no antimicrobial activity when maleic anhydride groups of SPM were aminated or hydrolyzed. The results of the repeated disinfection tests showed that the SR coated with SPM could maintain 87.3% bactericidal activity within 5 cycles. Furthermore, the SPM coating only imparted slight toxic effect (>85% viability) on L929 cells after 36 h of coculture, which is superior to the coating of aminated SPM conjugated with the antimicrobial peptide E6. The terpolymer containing maleic anhydride units have great potential as a flexible and durable coating against implant infections.
研究领域:
材料科学
·
生物医学工程
·
微生物学
·
表面化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 30
·18448-18457
·
IF 3.361
Ying Zhao,Xi He,Han Wang,Jiufu Zhu,Huimin Wang,Yan Zheng,Song Zhu,Zhanchen Cui
Hydrolysis resistance is essential to the durability of the dentin bonding interface. Urushiol is a natural monomer that has been used in different fields over thousands of years but has the disadvantage of a long drying time. In this study, we evaluated a novel photocurable derivative of urushiol as the main monomer for polymerization in dentin adhesive and its effect on hydrolysis resistance. The derivative was characterized by Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy. Compared with the Adper Single Bond 2, the experimentally synthesized adhesives had higher contact angles. In particular, the water sorption/solubility of the experimental samples were significantly lower than that of Adper Single Bond 2. The microtensile bond strengths of the test groups were higher than that of the control group, even after 5000 thermocycles. Cytotoxicity test results showed that adhesives based on the original derivative induced low toxicity to L929 cells. The results of this study may shift the focus of future research to natural monomers and even their derivatives which may perform well in dentistry.
研究领域:
牙科学
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材料科学
·
高分子化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Zexiang Zheng,Xing Yang,Yifan Zhang,Weiqiang Zu,Minna Wen,Taisheng Liu,Changren Zhou,Lihua Li
To achieve the pH-responsive release of metformin in tumor acidic microenvironment, we prepared OHA-Met by covalently grafting metformin (Met) onto oxidized hyaluronic acid (OHA) through imine bonds, and then prepared carboxymethyl chitosan (CMCS)/OHA-Met drug loaded hydrogels. The CMCS/OHA-Met hydrogels showed the in-situ injection performance. At pH = 7.4, the cumulative release rate of metformin from CMCS/OHA-Met20 hydrogel was 42.7 ± 2.6 % in 6 h, and the release tended to balance after 72 h. At pH = 5.5, the release kept constant and the cumulative release rate was 79.3 ± 4.7 % at 6 h, showing good pH-responsive behavior. Metformin induced apoptosis of MCF-7 cells through the caspase 3/PARP pathway. CMCS/OHA-Met20 hydrogel could effectively kill MCF-7 cells, while reducing the cytotoxicity of free metformin to L929 cells. In vivo breast cancer recurrence experiments showed CMCS/OHA-Met20 hydrogel could achieve local injection and pH-responsive smart drug delivery at the tumor resection site, inhibiting breast cancer recurrence. Compared with direct administration, CMCS/OHA-Met20 hydrogel reduced the metformin dosage, frequency of administration and systemic side effects.
研究领域:
药学
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生物医学工程
·
癌症研究
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Guohua Qi,Haijuan Li,Ying Zhang,Chuanping Li,Shuping Xu,Minmin Wang,Yongdong Jin
Cytochrome c (Cyt c) release and cellular pH change are two important mediators of apoptosis. Effective methods to regulate or monitor such two events are therefore highly desired for apoptosis research and cancer cell therapy. Herein, we exploited electrostimulation to regulate cellular Cyt c release and apoptosis process, and by designing and preparing a smart and efficient plasmonic nanorobot (with surface-modified Cyt c-specific aptamer and 4-mercaptobenzoic acid) that is capable of Cyt c capture and self-sensing, we achieved real-time SERS monitoring of dynamic Cyt c release and simultaneous cell acidification in apoptosis during electrostimulation. Distinctly different molecular stress responses in the two events for cancerous MCF-7 and HeLa cells and normal L929 cells were identified and revealed. The method and results are valuable and promising for apoptosis and Cyt c-mediated biology studies.
研究领域:
生物医学工程
·
纳米技术
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癌症研究
·
细胞生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Jie Liu,Qin Zou,Chenxin Wang,Mingyue Lin,Yufan Li,Rui Zhang,Yubao Li
The guided bone regeneration (GBR) concept has been extensively utilized to treat periodontal defects in clinical practice. However, the repair efficacy of the currently available GBR membranes is often compromised by their limited alveolar bone regeneration potential, insufficient mechanical strength and inadequate space maintenance duration. In this paper, a novel hybrid bi-layer scaffold was fabricated using electrospun polycaprolactone/gelatine (PCL/Gel) nanofibre membranes combined with 3D printed PCL/Gel/nano-hydroxyapatite (n-HA) scaffolds. Their manipulation parameters and microstructures were investigated, and biological studies of in vitro and in vivo bone regeneration were carried out. The heparin-conjugated PCL/Gel fibrous membrane could significantly promote L929 fibroblast adhesion and proliferation. The PCL/Gel/n-HA (PGH) scaffold could promote attachment, growth and osteogenic differentiation of BMSCs. After 20 weeks, the defect sites in the hybrid bi-layer scaffold group showed a higher degree of new bone formation than that in the control group, indicating that this is a promising material combination for GBR.
研究领域:
生物材料
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组织工程
·
牙科材料
·
再生医学
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产品关联
DOI
高影响因子
LANGMUIR
·
2023
·
IF 3.9
Xingrui Qi,Jiamin Gan,Zhidong Zhao,Nian Li,Yufang Chen,Tao Jin
One of the potential treatments for bacterial wound infections is photodynamic therapy. WO3–x semiconductor materials can generate reactive oxygen species when exposed to light, which can inactivate bacteria. In this work, we improved their photocatalytic performance by doping WO3–x with Cu. The wound dressing was prepared by loading Cu–WO3–x into a highly biocompatible chitosan sponge. The composite sponge dressing showed significant inactivation of Escherichia coli and Staphylococcus aureus, and in vitro toxicity assays on L929 cells demonstrated the biosafety of the dressing. Through in vivo wound healing trials, composite sponge dressings have been shown to accelerate wound healing, and this composite chitosan sponge can be possibly used for photodynamic therapy of bacterial wound infections.
研究领域:
材料科学
·
生物医学工程
·
光催化
·
伤口护理
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 12
·2545-2553
·
IF 3.286
Du Fangkai,Li Mengru,Wei Yanye,Huang Donglian,Zhou Yan,Yang Lifang,Chen Jian,Liu Shaogang,Tan Xuecai
The design of vigorous tools for creatinine determination is extremely important in the diagnosis and treatment of kidney diseases. In the study, we examine a robust fluorescent turn-on probe (NCP-Pd) for creatinine detection in a completely aqueous solution based on the metal palladium-catalyzed reaction. In the presence of creatinine, the NCP-Pd dissociates and subsequently restores the fluorescence due to elimination of the heavy atom quenching effect and prevention of the photoinduced electron transfer effect. The probe NCP-Pd displays excellent detecting performance with respect to creatinine such as good water solubility, high selectivity, and a low detection limit (0.16 μM). Additionally, in order to ensure its clinical application, this probe is operated in blood serum samples for detecting creatinine and compared with a commercial clinical method. The results indicate an extremely high agreement with the commercial clinical method. Furthermore, the results confirm that the probe NCP-Pd exhibits satisfactory cell permeability and low cytotoxicity and can detect creatinine in L929 and HCT116 cells. The study provides a potential application for detecting creatinine and conducting pathological research on creatinine-involved diseases.
研究领域:
分析化学
·
生物化学
·
生物医学工程
·
细胞生物学
·
临床化学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2023
·
IF 6.6
Xiaoxia Zhang,Changkai Yang,Xin Guo,Chun Yang,Guoying Li
The development of antibacterial dressings has attracted much attention to address the disordered wound healing caused by bacterial infection. Constructing dressings that have desirable antibacterial activity and could promote wound healing is important for infected wound repair. Inspired by the role of the key regulator collagen fibrils with D-periodic functional domains in physiological wound healing process, we developed an antibacterial and wound healing-promoting collagen fibril with a structure highly similar to natural collagen in ECM and inherent antibacterial activity by the simultaneous strategy of fibril reconstitution and the antibacterial agent ε-polylysine (ε-PL) anchoring. Accompanied by the fibrillogenesis of collagen molecules, the anchorage of ε-PL into collagen fibrils was actualized through the formation of the covalent bond catalyzed by transglutaminase (TGase) between ε-PL and collagen. The collagen fibril possessed natural D-periodicity and achieved 20% ε-PL graft yield by co-assembling collagen/ε-PL mediated by TGase of 25 U/g, which showed a satisfactory proliferation of L929 fibroblasts and sustained inhibition rates above 90% against E. coli and S. aureus. The rats’ S. aureus-infected dermal wound model further demonstrated that the antibacterial reconstituted collagen fibril visibly promoted re-epithelialization, new collagen deposition, and angiogenesis by down-regulating the inflammatory-relative genes IL-6 and up-regulating relative activity factor expression of CD31, achieving accelerated infected wound healing with 61.89% ± 3.96% wound closure on postoperative day 7 and full closure on day 14.
研究领域:
生物材料
·
伤口愈合
·
生物医学工程
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
·
2023
·
IF 3.9
Mengfan Zhang,Xiaotong Peng,Yansheng Huang,Kai Li,Jing Zhang,Pu Xiao,Yingshan Zhou
3D printing is an attractive method to accurately construct artificial organs or alternative materials with complicated structures and functional performance. Naturally derived hydrogels have emerged as promising materials for the preparation of biomimetic 3D organization or scaffolds by 3D printing due to their good biocompatibility, high water content, and fascinating 3D network. However, the poor printing properties and weak structural stability of naturally derived hydrogels limit their applications. In this study, photopolymerizable hydrogels are designed based on maleic chitosan (MCS) and thiolated sodium hyaluronate (SHHA). The Michael addition between MCS and SHHA improves the viscosity of the mixed solution. Moreover, it benefits the 3D printing process, followed by photopolymerization (acrylate-thiol step-chain polymerization and acrylate–acrylate chain polymerization) to form a stable covalent network rapidly. The rheological property, swelling behaviors, microstructure, and in vitro degradation are tuned by adjusting the molar ratio of the thiol group and acrylate group. In addition, MCS/SHHA hydrogel scaffolds with good accuracy and enhanced structural stability are prepared using extrusion-based 3D printing and photopolymerization technology. The hydrogels display excellent cytocompatibility and can support adherence of L929 cells, which can be used as prospective materials for tissue engineering applications.
研究领域:
材料科学
·
生物医学工程
·
组织工程
·
3D打印
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）