FOOD AND AGRICULTURAL IMMUNOLOGY
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2019
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IF 2.398
Zijian Chen,Kai Zhou,Wuzu Ha,Peihong Chen,Huijun Fu,Yudong Shen,Yuanming Sun,Zhenlin Xu
Melatonin (MT) is a hormone secreted by the pineal gland which regulates sleep rhythm and is widely used in health foods. However, MT is sometimes added illegally and excessively in certain health foods, which might cause undesirable side-effects. In this work, an easy-to-use immunochromatographic assay (ICA) based on mouse monoclonal antibody (mAb) for rapid detection of MT was developed for the purpose of safety. Under the optimal conditions, the developed ICA exhibited a visual limit of detection (VLOD) of 10,000 ng/mL and a LOD of 50 ng/mL (strip reader) with the linear range from 50 ng/mL to 5000 ng/mL. Good correlation was obtained between the results of ICA and standard liquid chromatography (LC) in matrix test and blind samples test. The developed ICA can be applied for rapid detection of samples within 30 min, and is potentially an excellent tool for screening of MT in health foods.
研究领域:
分析化学
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食品科学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
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IF 6.393
Di Shao,Shuyun Bi,Rui Zhao,Xiaoyue Sun,Xu Li,Jingfu Yu
Two sensitive surface-enhanced Raman spectroscopy (SERS) analytical methods for dinitolmide and toltrazuril were developed by silver nanoparticles (AgNPs). The dinitolmide and toltrazuril were determined in 0.0565 mol L −1 and 0.0847 mol L −1 NaOH solutions respectively. The enhancement factors (EF) were 1.76 × 10 5 for dinitolmide and 1.27 × 10 5 for toltrazuril. Under optimum conditions, the methods had good linear relationship in the concentration ranges of 0.30–33.30 nmol L −1 for dinitolmide ( I SERS = 1522.52 c + 27,890.46, c :nmol L −1 , R 2 = 0.998) and 0.30–66.70 nmol L −1 for toltrazuril ( I SERS = 1334.29 c + 46,347.83, c :nmol L −1 , R 2 = 0.999). The limits of detection (LOD) (S/N = 3) were 0.915 nmol L −1 for dinitolmide and 1.03 nmol L −1 for toltrazuril. The effects of interferences on the determinations of dinitolmide and toltrazuril were studied. These SERS methods were successfully used to measure the content of dinitolmide and toltrazuril in the duck and chicken samples. The relative standard deviation (RSD) for the detection of dinitolmide and toltrazuril samples could reach 1.45 % ( n = 5). The recoveries were 95.67%–105.39% ( n = 5) and 94.79%–99.44% ( n = 5) for dinitolmide and toltrazuril system respectively.
研究领域:
分析化学
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兽医学
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纳米技术
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产品关联
DOI
高影响因子
TALANTA
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2022
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IF 6.556
Xin Wang,Min Zhang,Xiaoxu Pang,Kunlun Huang,Zhiyi Yao,Xiaohong Mei,Nan Cheng
Staphylococcus aureus is a frequent pathogenic bacterium that has a significant detrimental influence on the health of the human body. Therefore, developing a practical and portable detection platform is critical to ensuring food safety. Nanozymes are a kind of engineered nanomaterials with superior enzyme-like activities, providing infinite possibilities for the development of highly sensitive analytical assays. In this study, mesoporous core−shell [email protected] ( [email protected] ) nanozymes were synthesized and then applied as a signal amplifier in Staphylococcus aureus colorimetric immunoassay . At the same time, a careful comparative study of the catalytic performance with natural horseradish peroxidase (HRP), [email protected] nanozymes and their complexes [email protected] (HRP coupling with [email protected] nanozymes) were firstly performed, as well as clever using a colorimeter to achieve portable signal output. [email protected] bioprobes enable remarkable peroxidase-like catalytic activity, resulting in the highest sensitivity with a limit of detection (LOD) improved from 1 × 10 5 to 1 × 10 3 CFU/mL. In all, this work conducted a valuable comparative study and presented an improved strategy for the portable detection of pathogenic bacteria, which was expected to be used in a wide range of applications in food safety regulation and biomedicals arenas.
研究领域:
分析化学
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生物技术
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食品安全
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生物传感器
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纳米技术
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2023
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IF 6.1
Chihong Ma,Qin Zhou,Jinjin Shi,Hua Gao,Di Huang,Huimin Xue,Han Wang,Zhenzhong Zhang,Sen Yang,Junli Zhang,Kaixiang Zhang
The clustered regularly interspaced short palindromic repeats (CRISPR) system provides a new molecular diagnostic tool for construction of biosensor platforms due to its high programmability and target specificity. Herein, we developed a CRISPR-empowered electrochemical biosensor by combining the advantages of CRISPR/Cas13a and primer exchange reaction (PER), named PER-E-CRISPR, for target amplification-free and sensitive detection of miR-21. Dual-signal amplification procedures involve the binding of target miR-21 induced by CRISPR-based amplification, along with the hybridization of multiple short single-stranded DNA strands with PER concatemers. When target miR-21 is present, CRISPR/Cas13a specifically recognizes the target miRNA , triggering the trans -cleavage activity of CRISPR/Cas13a. Then Cas13a/crRNA/miRNA cleaved the predesigned ribonucleotide site in hairpin 1 (HP1) and released trigger to open hairpin 2 (HP2) modified on the electrode surface. Then PER bridge sequence contained in HP2 is exposed and hybridized with PER concatemers, following multiple short single-stranded DNA tagged with methylene blue (ssDNA-MB) bond with the PER concatemers. Under optimized conditions, PER-E-CRISPR assay for detecting miR-21 exhibits linearity in dynamic range from 10 −13 to 10 −7 M, and we obtained a limit of detection (LOD) of 30.2 fM. The established PER-E-CRISPR biosensor shows perfect practical performance in actual plasma, which may have great promising prospects for miRNA detection in the field of molecular diagnosis.
研究领域:
分子诊断
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生物技术
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电化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
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2024
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IF 5.6
Xiaowei Chen,Yujun Feng,Yuanyuan Wang,Xin Yang,Xingping Zhang,Hualin Yang,Yu Zhou
With the gradual increase of oxytetracycline (OTC) residue in the environment, it is particularly important to establish a sensitive and efficient method to detect OTC. In this work, a novel colorimetric and fluorescence dual-mode detection method based on hemin/G-quadruplex (hemin/G4) DNAzyme and zeolitic imidazolate framework-8 (ZIF-8) is developed. OTC can compete with G4 to bind hemin to reduce the amount of hemin/G4. Thus, the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to blue color by hemin/G4 is inhibited. In addition, the OTC or OTC-hemin can be aggregated with the help of ZIF-8 to produce strong green fluorescence due to the aggregation-induced emission (AIE) effect. In colorimetric and fluorescence mode, the linear ranges of the new method are 0.375–18 μM and 0–52.5 μM, with a limit of detection (LOD) of 0.116 μM and 0.040 μM, respectively. Besides, this new method has a good selectivity and recovery rate in the detection of real samples. Therefore, the method can be used for the efficient and sensitive detection of OTC.
研究领域:
分析化学
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环境科学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
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2022
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IF 6.556
Ting Zhou,Guangzhao He,Changjia Hu,Kang Wu,Ying Liu,Jianguo Li,Anping Deng
5-Fluorouracil (5-FU) is an effective anticancer drug widely used in cancer treatment. In this study, two 5-FU derivatives containing a spacer arm with the carboxylic group at the end were synthesized, which were linked to the carrier proteins to form 5-FU-protein conjugates used as the immunogens for the production of monoclonal antibody (mAb). Based on the produced mAb, the highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for 5-FU detection was established. The IC 50 and LOD values of the assay were found to be 19.5 ng mL −1 and 0.5 ng mL −1 , respectively. There was no cross-reactivity (CR) of the ELISA with cytosine , thymine and uracil , which avoided the interference from inherent pyrimidines. The CR values of the assay with three substitutes of 5-FU (tegafur, 5-fluoro-2′-deoxyuridine, carmofur) were within 9.7%–17.6%. The produced mAb was also applied in sample extraction. The immuno-affinity column capable specific capturing 5-FU was prepared by immobilizing the mAb on Sepharose-4B gel and filling into a SPE column. The recoveries of 5-FU in spiked samples measured by ELISA were 72.4%–90.7% with RSD of 3.6%–8.3%. Five blood samples collected from patients were extracted by immuno-affinity column, then measured by ELISA and confirmed by HPLC-MS/MS. There was a good correlation between HPLC-MS/MS and ELISA. It is demonstrated that the developed ELISA combined with immuno-affinity extraction can be a powerful alternative method for the detection of 5-FU in blood samples.
研究领域:
药理学
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分析化学
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免疫学
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临床化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Beibei Lu,Jialei Bai,Jingyang Zhang,Huijing Shen,Minglin Wang,Yujing Lian,Zhixian Gao,Yuan Peng
Colorimetric sensing materials have a rising demand in detection applications, especially in the field of naked-eye detection. Herein, a novel iridescent polymeric film that can respond to different Staphylococcus aureus enterotoxin B (SEB) concentrations in the visible range is reported. This film is fabricated by polymerization of methacrylic acid (MAA) and ethylene dimethacrylate (EGDMA) owing an inverse opal array (IOA) structure, and gold nanoparticles (AuNPs) are enchased on the polymer skeleton. This composite structure facilitates the film to respond to SEB at a LOD of 4.29 fg mL −1 , making it the best performing colorimetric material to date. Cryo-Scanning Electron Microscopy and 3D AFM were used to observe the morphology change of the iridescent polymeric film after SEB binding. This film possesses great advantages for ultrasensitive visual detection of real samples. In addition, the iridescent polymeric film has the potential to become a universal sensing material, realizing the ultrasensitive visual detection of hazardous substances without equipment in the field by replacing the recognition element.
研究领域:
材料科学
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分析化学
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生物传感
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纳米技术
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Hong Chen,Junyan Liu,Mengna Wan,Shangyi Zhao,Xiaolong Li,Chuan Liu,Xuanping Zhao,Junjie Li,Qingzhen Ma,Cuixiang Li,Xueqin Wang
Fruit juice is kind of popular beverage food worldwidely, and its total antioxidant capacity (TAC) is generally considered as an important index for people’s rational diet, thus accurate evaluation of its TAC is essential for human health guidance. Herein, we constructed a sensitive Fe 3 O 4 @MnO 2 nanocatalyst that was delicately cored with magnetite nanoparticles (Fe 3 O 4 NPs) and encapsulated with manganese dioxide (MnO 2 ) nanoshells for fast and accurate TAC colorimetric analysis in fruit juice. The data showed the fabricated Fe 3 O 4 @MnO 2 nanocatalysts performed with remarkable oxidase mimetic activities and could catalyze oxidization of colorimetric substrate without additional H 2 O 2 , and the limit of detection (LOD) was about 0.39 μM for ascorbic acid (AA) evaluation. Applied in commercial fruit juice products, the fabricated Fe 3 O 4 @MnO 2 nanocatalysts showed a reliable and visual assessment for the accurate TAC analysis. In addition, the fabricated Fe 3 O 4 @MnO 2 nanocatalysts could be easily recycled due to their intrinsic superparamagnetic property. Together, an intellegent Fe 3 O 4 @MnO 2 nanocatalyst was well developed for visual TAC analysis in fruit juice with convenient applications in future.
研究领域:
纳米技术
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分析化学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2024
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IF 3.8
Xingchen He,Feixia Peng,Yihan Ma,Hongyu Wang,Xiaoyang Mo,Yani Ma,Xin Tang,Qian Li,Xueqin Ding
Wild animals, as a vital component of our natural world, serve a crucial role in preserving ecological equilibrium and biodiversity. By delving into the genetic constitution of wild animal populations, the evolutionary history, genetic diversity, and adaptation mechanisms could be explored, thereby informing conservation strategies and safeguarding the future of these species. In order to study the genetic information of wild animals, it is necessary to extract high purity and high concentration of wild animal genomic DNA. In this work, a hydrophobic magnetic deep eutectic solvent (HMDES) based vortexed extraction was developed for the extraction of genomic DNA from leopard cat ( Prionailurus bengalensis ), hairy-crowned deer ( Elaphodus cephalophus ) and muntjac ( Muntiacus reevesi ) muscle tissue, respectively. Extraction conditions like the pH value, extraction time, temperature and the amount of HMDES used were optimized by single-factor experiments. Under the optimized condition, genomic DNA could be selectively extracted from the three animal tissues. The limits of detection (LOD) and limits of quantification (LOQ) of the proposed method were 2.86 ng/μL and 8.66 ng/μL, respectively. Meanwhile, the relative standard deviation (RSD) of the method precision and repeatability were 1.64 % and 5.57 % at 20 ng/μL, showing the method has good precision and repeatability. After extraction, the DNA extracted into the HMDES droplets can be quickly recovered and the HMDES can be recycled and reused. The method proposed is a fast, environmental-friendly and high efficient extraction strategy for purification and enrichment of genomic DNA from leopard cat, hairy-crowned deer and muntjac tissues.
研究领域:
遗传学
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生物化学
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保护生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Xue Zhang,Yamin Yu,Kaixin Du,Wenwei Han,Jie Xue,Shanshan Li,Shangyou Lv,Jianye Fu,Xin Wang,Meng Qiu
The global pandemic of coronavirus disease 2019 (COVID-19) highlights the shortcomings of the current testing paradigm for viral disease diagnostics. Though the Polymerase Chain Reaction (PCR) technique is currently used for nucleic acid detection in liquid samples, its application in airborne virus has not yet been developed, due to low viral load in the air, lack of efficient capture probe and supporting equipment. Herein, we developed an ultrasensitive biosensing nanoplatform for airborne virus detection. The extraction and concentration of nucleic acid from test samples has been accomplished by novel spike silica nanoparticles (SSNPs)-based nucleic acid capture probe with high spike length to nanoparticle diameter ratio and appropriate surface potential. When integrated with air sampler and Quantitative Real-Time PCR (qPCR) equipment, nucleic acid enrichment can be achieved significantly. Our results demonstrated a successful detection of the virus in the air with the limit of detection (LoD) of 500 copies/L, which greatly surpasses the LoD of 1 × 10 6 copies/L. This detection method effectively addresses the obstacle posed by the low abundance of pathogens in the environment and could be applied for the detection of pathogens in air or water, aiming to achieve the goal of disease prevention and control prior to its onset.
研究领域:
纳米技术
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病毒学
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生物医学工程
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分析化学
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公共卫生
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Hui Zhao,Feng Liu,Wei Xie,Tai-Cheng Zhou,Jun OuYang,Lian Jin,Hui Li,Chun-Yan Zhao,Liang Zhang,Jia Wei,Ya-Ping Zhang,Can-Peng Li
The recent pandemic outbreak of COVID-19 caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses a threat to public health globally. Thus, developing a rapid, accurate, and easy-to-implement diagnostic system for SARS-CoV-2 is crucial for controlling infection sources and monitoring illness progression. Here, we reported an ultrasensitive electrochemical detection technology using calixarene functionalized graphene oxide for targeting RNA of SARS-CoV-2. Based on a supersandwich-type recognition strategy, the technology was confirmed to practicably detect the RNA of SARS-CoV-2 without nucleic acid amplification and reverse-transcription by using a portable electrochemical smartphone. The biosensor showed high specificity and selectivity during in silico analysis and actual testing. A total of 88 RNA extracts from 25 SARS-CoV-2-confirmed patients and eight recovery patients were detected using the biosensor. The detectable ratios (85.5 % and 46.2 %) were higher than those obtained using RT-qPCR (56.5 % and 7.7 %). The limit of detection (LOD) of the clinical specimen was 200 copies/mL, which is the lowest LOD among the published RNA measurement of SARS-CoV-2 to date. Additionally, only two copies (10 μL) of SARS-CoV-2 were required for per assay. Therefore, we developed an ultrasensitive, accurate, and convenient assay for SARS-CoV-2 detection, providing a potential method for point-of-care testing.
研究领域:
生物技术
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电化学
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即时诊断
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病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2023
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IF 2
Qing Zhang,Chunrong Zhang,Nan Li,Zhao Dai
Aptamer-based biosensors have been demonstrated to provide real-time physical and chemical signals for biomedicine, food safety, drug identification, and metal ion determination. In this study, cadmium ion (Cd2+) was determined in drinking water by color signaling using the specific binding of the aptamer and target by its regulation of the catalytic capacity of gold nanoparticles (AuNPs). When sodium borohydride (NaBH4) and a probe with a catalytic function were present, the solution containing 4-nitrophenol changed from yellow to colorless. Under optimal conditions, the naked eye was sufficient to determine Cd2+ with a limit of detection (LOD) of 5 μM. The discoloration time showed a positive correlation with Cd2+ concentration up to 30 μM. This method was label-free, efficient, rapid, and selective for Cd2+ in water without expensive instruments, suggesting practical applications.
研究领域:
分析化学
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环境科学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）