Acta Pharmaceutica Sinica B
·
2025
·
IF 14.6
Yinying Pu,Shaorong Huang,Shuang Gao,Yangying Duan,Wenhao Li,Qiyue Li,Han Lin,Kun Zhang,Min Zhou,Wencheng Wu
Simultaneous management of intestinal mucosal barrier dysfunction and gut microbiota dysregulation represents a significant challenge in the treatment of inflammatory bowel disease (IBD). Herein, we report a novel system that integrates multi-enzyme mimicking cerium single-atom nanocatalysts (CeSACs) with Lactobacillus reuteri probiotics (LR@CeSACs) for multipronged management of IBD. In this system, CeSACs demonstrate robust multi-enzyme activities across a broad pH range, effectively scavenging elevated reactive oxygen species, downregulating pro-inflammatory cytokines, and suppressing the expression of fibrosis-related genes. Moreover, probiotics promote the targeting and retention of the CeSACs for sustained catalytic antioxidant therapy. In turn, the inflammation relief enabled by CeSACs promotes bacterial viability, allowing for the rapid reshaping of intestinal barrier function and the restoration of gut microbiota. Therefore, LR@CeSACs exhibit excellent catalytic anti-inflammatory and anti-fibrotic therapeutic effects, as well as a certain prophylactic effect, as demonstrated in several murine models.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Zishen Lin,Jianmin Wu,Jinping Wang,Crystal L. Levesque,Xi Ma
Lactobacillus reuteri (L. reuteri) has been recognized as a probiotic to improve liver health, whereas the mechanism behind is still unknown. Herein, mice were gavaged with a strain of L. reuteri named SLZX19-12, which was isolated from faeces of healthy Tibetan pigs in our lab to investigate its effects on liver function. The results demonstrated that dietary L. reuteri changed the constituents of bile acid pool, especially influenced on metabolism of ursodeoxycholic acid (UDCA). In addition, L. reuteri reduced content of cholestasis-associated microbiota and effectively prevented liver from inflammatory response and hepatocyte apoptosis under the acute stimulation from LPS. We further revealed UDCA played an important part in protection of liver health during the stimulation of inflammatory factors in vitro . This study provides theoretical support and novel mechanism of action of L. reuteri protects liver from injury and influences on host physical health via regulation of crosstalk between bile acids and gut microbiota.
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Food & Function
·
2024
·
IF 5.1
Jiayi Zhang,Chengcheng Zhang,Leilei Yu,Fengwei Tian,Wei Chen,Qixiao Zhai
Gastrointestinal inflammation and intestinal barrier function have important effects on human health. Alcohol, an important foodborne hazard factor, damages intestinal barrier, increasing risk of disease. Lactobacillus reuteri strains have been reported to reduce gastrointestinal inflammation and strengthen the intestinal barrier. In this study, we selected three ant-inflammation L. reuteri strains to evaluate their role in the protection of the intestinal barrier and their immunomodulatory activity in a mouse model of gradient alcohol intake. Among the three strains tested (FSCDJY33M3, FGSZY33L6, and FCQHCL8L6), L. reuteri FSCDJY33M3 was found to protect the intestinal barrier most effectively, possibly due to its reduction on the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha (TNF-α) and increase on the expression of tight junction proteins (occludin, claudin-3). Genomic analysis suggested that the protective effects of L. reuteri FSCDJY33M3 may be related to functional genes and glycoside hydrolases associated with energy production and conversion; amino acid transport and metabolism; carbohydrate transport and metabolism; and DNA replication, recombination, and repair. These genes include COG2856, COG1804, COG2071, and COG1061, which encode adenine deaminase; acyl-CoA transferases; glutamine amidotransferase; RNA helicase; and glycoside hydrolases, including GH13_20, GH53, and GH70. Our results identified functional genes that may related to protection of alcohol-induced intestinal barrier damage, which might be useful for screening lactic acid strains that can protect the intestinal barrier.
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Nutrients
·
2026
·Vol. 18
·期 11
·1768
·
IF 5.8
Yingshan Jiang,Xinyu Wei,Jingchen Yan,Yanchao Xing,Yanlu Li,Aoyun Li,Yue Teng,Ting Ren,Weijia Chen,Jianming Li,Ying Zong,Rui Du
Background/Objectives:Osteoporosis (OP) is a frequent complication of rheumatoid arthritis (RA), driven by chronic inflammation and subsequent bone destruction. While deer bone is recognized for its potential bone-health benefits, the therapeutic efficacy of its fermented products on RA-induced OP remains unclear.Methods:This study investigated the protective effects ofLactobacillus reuteri-fermented deer bone water extract (LR-DBW) against OP in an adjuvant arthritis (AA) rat model. Firstly, LC-MS/MS was employed to analyze the differential peptide profiles between LR-DBW and non-fermented deer bone water extract (DBW). Secondly, experiments such as Micro-CT, histological staining, and Western Blot were conducted to detect the improvement effect of LR-DBW on OP.Results:In vivo, LR-DBW administration significantly alleviated arthritis symptoms, increased bone mineral density (BMD), and improved bone microarchitecture in AA rats.In vitro, LR-DBW inhibited RANKL-induced osteoclastogenesis and actin ring formation in RAW264.7 cells. Mechanistically, LR-DBW inhibits the phosphorylation of ERK, JNK and p38 proteins.Conclusions:These research results indicate that one of the mechanisms by which inhibiting osteoclast differentiation into LR-DBW can alleviate osteoporosis caused by rheumatoid arthritis is through down-regulating the phosphorylation expression of ERK, JNK and p38 proteins. This highlights its potential as a functional food component for treating inflammatory bone diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Jiaqi Chen,Jiayue Zhao,Yibin Xu,Xianglin Fei,Shujie Xu,Binke Chen,Wenzi Wu,Jiachen Zhuang,Xiuan Zhan,Aikun Fu
Enteric Lactobacillus plays a crucial role in maintaining the intestinal barrier integrity. Lactobacillus reuteri , one of the most described probiotic species of Lactobacillus , has been shown to alleviate enteritis-associated intestinal mucosal damage by stimulating the expansion of intestinal stem cells (ISCs). However, the underlying ligand-protein interactions remain unexplored. Therefore, this study aimed to elucidate the specific molecular mechanisms by which L. reuteri and its metabolites govern ISC stemness. We employed broiler enteritis model and organoid culture to compare the probiotic effect of a wild-type (WT) L. reuteri strain and a reuterin-deficient isogenic mutant. Compared to the WT strain, the deficient strain exhibited an impaired ability to alleviate inflammation, restore the intestinal barrier, and stimulate ISC proliferation both in vivo and ex vivo, indicating that reuterin is the essential effector. Furthermore, bioorthogonal click chemistry combined with in silico molecular docking were utilized to map the direct host protein targets. We identified protein disulfide-isomerase 1 (PDIA1), an endoplasmic reticulum (ER)-resident redox-active protein, as the direct binding partner of reuterin, characterized by a strong binding affinity. The inactivation of PDIA1 by reuterin binding increased ER-derived reactive oxygen species (ROS) level, leading to the upregulation of Wnt/β-catenin pathway and the maintenance of ISCs. In conclusion, by inactivating the ER-resident PDIA1, L. reuteri -derived reuterin promoted ISC proliferation and ameliorated intestinal mucosal damage.
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Journal of Cleaner Production
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2019
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IF 6.395
Hossain M. Zabed,Yufei Zhang,Qi Guo,Junhua Yun,Miaomiao Yang,Guoyan Zhang,Xianghui Qi
The probiotic bacterium, Lactobacillus reuteri, possesses a unique pathway for metabolizing glycerol into 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PD), which are two important platform chemicals. In this work, a potent L. reuteri strain, designated as FXZ014, was isolated and identified from healthy human feces for co-biosynthesis of 3-HP and 1,3-PD during whole cell biotransformation of glycerol. Initially, biotransformation conditions were optimized for this new strain using single factor optimization and response surface methodology (RSM). Under the optimum batch conditions, FXZ014 produced 8.74 ± 0.24 g/L of 3-HP and 9.94 ± 0.36 g/L of 1,3-PD with a corresponding net yield of 0.67 mol/mol. Finally, efficiency of the isolated strain in the co-production of these two metabolites was compared with the same of a reference strain, namely L. reuteri CICC 6118. Compared to the reference strain, the amounts of 3-HP and 1,3-PD produced by the isolated strain were significantly higher that corresponded to 38.4% and 21.14%, respectively. Therefore, FXZ014 could be a promising natural strain for co-biosynthesis of 3-HP and 1,3-PD from glycerol.
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Food & Function
·
2023
·Vol. 15
·期 1
·387-400
·
IF 6.1
Chuanchuan Wang,Weixuan Chen,Yishan Jiang,Xiao Xiao,Qianhui Zou,Jiarui Liang,Yu Zhao,Qianxu Wang,Tian Yuan,Rui Guo,Xuebo Liu,Zhigang Liu
Autism Spectrum Disorder (ASD), a complex neurodevelopmental disorder marked by social communication deficits and repetitive behaviors, may see symptom amelioration through gut microbiota modulation. This study investigates the effects of a synbiotic – specifically a probiotic amplified by prebiotic supplementation – on ASD-like mouse model's social deficiencies. This model was established via valproic acid injection into pregnant females. Post-weaning, male progeny received daily synbiotic treatment, a combination of Lactobacillus reuteri (L. reuteri) and inulin, for four weeks. Results indicated that the synbiotic rectified social impairments and attenuated inflammatory cytokine expressions in the brain. Moreover, synbiotic intervention protected gut barrier integrity and altered the gut microbiota composition, enhancing the butyrate-producing Bifidobacterium abundance. The synbiotic elevated metabolites such as butyrate and 3-hydroxybutyric acid (3-HB), alongside upregulated genes associated with 3-HB synthesis in the colon and liver, and brain receptors. Conclusively, the synbiotic combination of L. reuteri and inulin mitigated ASD-related social impairments, partially via their regulatory effect on the gut–brain axis.
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