AQUACULTURE
·
2019
·
IF 3.022
Yafei Duan,Yun Wang,Xian Ding,Dalin Xiong,Jiasong Zhang
Succinate (SA) is the precursor of propionate that influence the intestine physiological function of animals. In this study, we investigated the microbiome , digestive, and immune responses in the intestines of Pacific white shrimp Litopenaeus vannamei fed diets containing different levels of SA: 0 g/kg (Control), 2.5 g/kg (SA1), 5.0 g/kg (SA2), and 10.0 g/kg (SA3) for 56 days. The results showed that dietary SA improved the intestine mucosa morphology, increased the short-chain fatty acids contents, and altered the intestine microbiota composition. Specifically, SA increased the abundances of Proteobacteria and decreased the abundance of Bacteroidetes . The beneficial bacteria including Ruegeria , Lutimonas , Mameliella , and Sphingomonas were enriched in response to dietary SA; these genus might be involved in nutrient metabolism and the degradation of toxic compounds and pollutants . Additionally, dietary SA also improved the activity of digestive enzymes (amylase, lipase , trypsin, and pepsin) and immune enzymes (total antioxidant capacity, phenoloxidase, total nitric oxide synthase , and nitric oxide), and the expression of immune-related genes (prophenoloxidase, anti-lipopolysaccharide factor, lysozyme , heat shock protein 70 , thioredoxin , mucin-1, mucin-2, mucin-5 AC, mucin-5B, and mucin-19) in the intestine of the shrimp. These results revealed that dietary SA had a positive effect on the intestine health of L . vannamei by modulating the microbial composition and SCFA contents, and enhancing the digestive and immune response in the shrimp intestine.
研究领域:
水产养殖
·
微生物学
·
营养学
·
免疫学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Kexin Zhang,Na Li,Junzhang Li,Yefan Wang,Chang Liu,Yuxin Liu,Xiaoyang Liu,Dayong Zhou,Deyang Li
The objective of this study was to investigate the effects of ultra-high pressure (UHP) on the physicochemical properties, gelling properties, and in vitro digestion characteristics of myofibrillar protein (MP) in Litopenaeus vannamei . The pressures were set at 0, 150, 300, 450, and 600 MPa, respectively. The α-helix structure gradually unfolded and transformed into β-sheet structure as the pressure increased from 0 to 600 MPa. Compared with the control group, the content of α-helix structure of the 600 MPa-treated MP samples decreased by 51.31%, and the content of β-sheet structure increased by a 1.34-fold. In addition, the UHP treatment enhanced the rheological properties of MP. The elastic modulus (G′) and viscous modulus (G″) of the 600 MPa-treated MP samples increased by 4.77-fold and 3.78-fold, respectively, compared with the control group (at frequencies up to 10 Hz). The gel properties of the MP also increased significantly after UHP treatment, e.g., the WHC of MP gel increased by 45.49% and the gel strength increased by a 5.99-fold when the pressure reached 600 Mpa, compared with the control group. The results of in vitro simulated digestion showed that UHP treatment led to the formation of more easily digestible MP gels. Compared with the control group, the 600 MPa-treated MP gel samples showed a 1.89-fold increase in digestibility and a 66.86% decrease in digestible particle size.
研究领域:
食品科学
·
蛋白质化学
·
生物物理学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Kexin Zhang,Yefan Wang,Xin Fan,Na Li,Zhifeng Tan,Huilin Liu,Xiaoyang Liu,Dayong Zhou,Deyang Li
In this study, the effects of CaCl 2 (0, 25, 50, 75, and 100 mM) on the gelling and digestive properties of the myofibrillar protein (MP) in Litopenaeus vannamei were investigated. The results showed that increasing CaCl 2 concentration led to changes in the tertiary structure of MP. Specifically, compared with the control group, a 64.31 % increase in surface hydrophobicity and a 45.90 % decrease in the sulfhydryl group were observed after 100 mM CaCl 2 treatment. Correspondingly, the water holding capacity and strength of the MP gel increased by 24.46 % and 55.99 %, respectively. These changes were positively correlated with the rheological properties, microstructure pore size, and content of non-flowable water. The mechanical properties of MP gel were improved, and the microstructure became more compact with the increase in CaCl 2 concentration. Furthermore, the particle size of the digested MP gels decreased in the presence of CaCl 2 , which improved the digestion characteristics of MP gels.
研究领域:
食品科学
·
水产养殖
·
蛋白质化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Conghui Shan,Chenxin Gong,Yingying Yu,Weixia Zhang,Difei Tong,Kaiwen Hu,Yan Gao,Yang Shu,Xunyi Zhang,Guangxu Liu,Wei Shi
Buprofezin (BPFN), an insecticide that inhibits arthropod chitin synthesis, is widely applied in agriculture and increasingly detected in aquatic environments. Given the evolutionary conservation of chitin biosynthesis pathways, its potential impacts on non-target crustaceans remain poorly understood. In this study, we investigated the effects of environmentally relevant BPFN concentrations on the exoskeletal properties of the whiteleg shrimp Litopenaeus vannamei . Nanoindentation revealed significant reductions in exoskeletal stiffness and hardness following BPFN exposure. Consistently, chitin and total calcium content decreased, accompanied by reduced mineral density and cuticular volume as shown by histology and micro-CT imaging. These structural impairments were closely associated with altered patterns of molt-related physiological and biochemical indicators, including changes in glycogen storage, chitinase activity, carbonic anhydrase activity, and Ca 2 + dynamics, and metabolites annotated to steroid-related pathways. Integrated transcriptomic and metabolomic analyses further revealed that BPFN markedly suppressed amino sugar and nucleotide sugar metabolism, downregulating key enzymes required for chitin precursor generation. BPFN also interfered with ECM-receptor interactions and ER-associated protein processing, disrupting cuticular protein assembly, and perturbed TGF-β/BMP signaling pathways critical for calcium carbonate deposition. Correlation analyses linked these molecular disturbances with defects in chitin synthesis, matrix organization, and biomineralization. In summary, our findings demonstrate that BPFN exposure compromised the structural integrity and mechanical performance of L. vannamei exoskeleton by impairing chitin synthesis and recycling, disturbing cuticular protein processing, weakening calcium carbonate mineralization, and disrupting molt-associated physiological processes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2025
·Vol. 2025
·期 1
·6665220
·
IF 3.9
Shiping Yang,Leyuan Feng,Junliang Luo,Jichang Jian,Shuanghu Cai,Huiling Liu
Nuclear factor erythroid 2-related factor 2 (Nrf2) is an essential component in regulating oxidative stress. Sulforaphane (SFN) is a natural antioxidant and gene Nrf2 agonist that can increase the antioxidant capacity of the organism and reduce oxidative stress. However, research on the repair of oxidative stress damage by SFN in aquatic animals remains extremely scarce. In order to further explore the function and role of SFN in the repair of oxidative stress injury in aquatic animals, this study took Litopenaeus vannamei as the research object. We established an oxidative stress model of L. vannamei through 6% oxidized fish oil (OFO) feeding. Methods, such as RNA interference (RNAi), qPCR, histopathological analysis, and TUNEL assay, were used to detect the changes in the oxidative stress status of L. vannamei . The results showed that the expression of Nrf2 in the hepatopancreas of L. vannamei in the double-stranded RNA (dsRNA)- Nrf2 + SFN group was significantly higher than that in the dsRNA- Nrf2 group and control group at 24 h ( p < 0.05). The transcription levels of antioxidant and autophagy genes in the SFN group were significantly higher than those in the control group ( p < 0.05), and the expression of related genes in the dsRNA- Nrf2 + SFN group was also higher than that in the dsRNA- Nrf2 group. Histopathology showed that Nrf2 knockdown would aggravate hepatopancreatic apoptosis and vacuolation, while SFN treatment after Nrf2 knockdown could alleviate hepatopancreatic injury and apoptosis caused by OFO. The results indicated that SFN could repair the oxidative stress injury of L. vannamei induced by OFO by activating Nrf2 . This study investigated the role of SFN in alleviating and repairing the oxidative stress damage in L. vannamei caused by OFO, aiming to provide a theoretical basis for the research on the antioxidant effect of SFN and the regulation of the antioxidant capacity of shrimp.
研究领域:
海洋生物学
·
水产养殖
·
分子生物学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yejun Zhang,Yongzhen Wang,Hao Dong,Jiao Li,Jianan Sun,Xiangzhao Mao
Temperature fluctuations can negatively affect the quality of frozen shrimp. Research on novel cryoprotectants to replace traditional agents (phosphate, etc.) has become a hotspot. Our results indicated that L-Proline could reduce thawing losses, delay texture deterioration and improve the functional properties of myofibrillar proteins of shrimp. Thawing loss in the proline group (3.2 %) was significantly lower than that in the control (5.4 %) after 3 freeze-thaw cycles ( p < 0.05). Compared to Na 4 P 2 O 7 , proline had better permeability and greater ability to inhibit ice crystal growth and volume expansion. Through molecular simulations, we found that proline might inhibit ice crystal formation by forming glassy states with water. Hydrogen bonding between proline and water/ice played a major role, and only a small amount of proline was required to significantly reduce the ice crystal growth rate from 0.16 m/s to 0.06 m/s. Briefly, proline exhibited potential as a cryoprotectant for shrimp in temperature fluctuations.
研究领域:
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Na Li,Xin Fan,Yefan Wang,Kexin Zhang,Rong Liu,Yunpeng Xu,Zhifeng Tan,Wensi Xu,Dayong Zhou,Deyang Li
Astaxanthin (AST), the natural pigment in Litopenaeus vannamei , is susceptible to oxidation and isomerization, leading to the fading of the orange-red color in ready-to-eat (RTE) shrimps. This study specifically investigated the changes mechanism in AST content, including geometric and stereoisomers, as well as oxidation degradation, throughout the storage process of RTE shrimps. The results showed that the total amount of AST decreased by 46.76 % after 45 days of storage at 40 °C. The levels of geometric isomers (all- E , 9- Z , 13- Z ) and stereoisomers (3 S ,3′ S , 3 S ,3′ R , 3 R ,3′ R ) gradually decreased over time. Notably, 9- Z and 3 S ,3′ S isomers, known for their strong antioxidant activity, were reduced by 83.57 % and 61.64 % respectively. Additionally, AST underwent oxidative degradation, forming short-chain compounds (astaxanthinal or astaxanthinone), with the main products being Apo-14′-astaxanthinal and Apo-7-astaxanthinone DHA ester. These findings provide a theoretical foundation for further research on the degradation mechanism of AST, and offer valuable insights into the color protection of RTE shrimps.
研究领域:
食品科学
·
化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE INTERNATIONAL
·
2026
·Vol. 34
·期 5
·140
·
IF 3.5
Shehata Akram Ismael,El Basuini Mohammed F.,Shija Vicent Michael,Bakky Md. Akibul Hasan,Tran Ngoc Tuan,Zhang Ming,Li Shengkang
This study probed the impacts of dietary octacosanol supplementation at 0 (control), 6, 12, and 18 mg/kg diets on the growth parameters, intestinal enzyme activities, biochemical, antioxidant status, gene expression pattern, and hypoxia tolerance of whiteleg shrimp ( Litopenaeus vannamei , initial weight 1.49 ± 0.01 g, n = 3, total shrimp = 360) during a 60-day feeding trial. To assess growth efficiency, final weight, weight gain, and specific growth rate were recorded, which were significantly ( p < 0.05) improved with octacosanol supplementation, with optimal responses observed at 12 mg/kg. Feed conversion ratio decreased quadratically, reaching optimal efficiency at approximately 10.83 mg/kg. Intestinal enzyme activities (amylase, lipase, and protease) and antioxidant indicators (SOD, CAT, GPx, and TAC) were markedly enhanced at 12 mg/kg, while lipid peroxidation (MDA) was minimized. Hemolymph biochemical indices, including total protein, albumin, globulin, HDL-c, and lysozyme, increased significantly ( p < 0.05), whereas AST and ALT decreased. Furthermore, transcriptional expression of immune- and antioxidant-related genes ( Mn-SOD , CAT , GSH-Px , LYZ , and ProPO ) was upregulated, coinciding with elevated antioxidant capacity and immune responses. Shrimp fed octacosanol diets exhibited enhanced hypoxia tolerance, with LT 50 extended by approximately 30–40 min compared to the control. Collectively, the results demonstrate that dietary octacosanol at 12 mg/kg optimizes growth, physiological health, and stress resilience in L. vannamei , supporting its potential as a natural dietary additive for sustainable shrimp farming. Future studies are needed to explore octacosanol’s long-term effects on shrimp under various stress conditions, its molecular mechanisms, and its impact on other species, as well as its synergy with other feed additives.
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DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Weiliang Guan,Wenqian Nong,Xiaobo Wei,Renchi Chen,Zhihai Huang,Yuting Ding,Xiaoming Qin,Luyun Cai,Linchun Mao
Background Water-free transportation (WFT), as a novel strategy for express delivery of live shrimp ( Litopenaeus vannamei ), was recently developed. However, air exposure during this transportation arouses a series of abiotic stress to the shrimp. In this work, the influences of WFT stress on glycolysis and lipolysis metabolism and meat quality (umami flavor and drip loss) were investigated in comparison with the conventional water transportation (WT). Results Results showed that type II muscle fibers with the feature of anaerobic metabolism were dominated in shrimp flesh. In addition, the increments of intracellular Ca 2+ was detected in WFT and WT, which then activated the AMP-activated protein kinase (AMPK) pathway, and thereby promoted the consumption of glycogen, accumulation of lactate and lipolysis under the enzymolysis of hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and adipose triglyceride lipase (ATGL). Glycogen glycolyzed to latate. Meanwhile, ATP degraded along with glycolysis resulting in the generation of ATP-related adenosine phosphates such as inosine monophosphate (IMP) with umami flavor and phosphoric acid. More remarkable ( p < 0.05) physiological changes (except LDH and lactate) were observed in WFT compared to WT. Additionally, fatty acid profile also slightly changed. Conclusions The transport stress induced significant energy metabolism changes of shrimp flesh, and therefore effected the flesh quality. The intensifications of freshness (K value) of shrimp flesh were detected due to ATP degradation, which were more pronounced after WFT. However, the drip loss of shrimp flesh was more significantly increased ( p < 0.05) after WFT compared to WT. This article is protected by copyright. All rights reserved.
研究领域:
水产养殖
·
生物化学
·
代谢
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
·
2023
·
IF 3.7
Menglin Shi,Xinzhou Yao,Kangyuan Qu,Yucheng Liu,Beiping Tan,Shiwei Xie
This experiment investigated the impact of taurine supplementation in low fishmeal diets on growth, antioxidant capacity, immunity and intestinal health of Litopenaeus vannamei . In this experiment, five diets were formulated, of which the high fishmeal diet contained 25% fishmeal (HF) and the remaining four diets were a mixture of soybean protein concentrate and Clostridium autoethanogenum protein (CAP) replacing 15% fishmeal and supplemented with 0%, 0.2%, 0.4% and 0.6% taurine (LF, T1, T2, T3). A total of 800 healthy shrimp (0.32 ± 0.00 g) were randomly allocated into 5 groups, with each group consisting of 4 replicates and 40 shrimp per replicate. The shrimp were fed four times a day for 51 days. The results showed that there was no significant difference in weight gain rate and survival ( P ＞0.05). Dietary taurine supplementation significantly improved the antioxidant capacity of shrimp. Compared with LF group, the mRNA expression of alf (anti-lipopolysaccharide factor) and cru (crustins) in HF group were significantly up-regulated ( P ＜0.05). Compared with the HF and LF groups, the mRNA expression of pen3 (penaeidins 3) in T1, T2 and T3 groups was significantly up-regulated, and the mRNA expression of dorsal in T2 group was significantly up-regulated ( P ＜0.05). The mRNA expression of bcl-2 (B-cell lymphoma-2) in T2 group was significantly up-regulated than that in other groups ( P ＜0.05). The mRNA expression of cyt-c (cytochrome c) in LF group was significantly up-regulated than that in other groups ( P ＜0.05). Compared with LF group, the mRNA expression of tnf-a (tumor necrosis factor-alpha) in HF, T2 and T3 groups was significantly down-regulated ( P ＜0.05). The mRNA expression of caspase8 in HF and T2 group was significantly down-regulated than that in other groups ( P ＜0.05). Compared with LF group, the mRNA expression of caspase3 in T1 groups was significantly down-regulated ( P ＜0.05). Intestinal H&E stained sections showed that dietary taurine supplementation improved intestinal mucosal folds height, width and muscle layer thickness. In general, dietary taurine supplementation can improve the antioxidant capacity, immunity and intestinal health of Litopenaeus vannamei , and the optimal supplemental level is 0.4%.
研究领域:
水产养殖
·
营养学
·
免疫学
·
海洋生物学
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DOI
高影响因子
JOURNAL OF FISH DISEASES
·
2021
·Vol. 45
·期 2
·349-359
·
IF 2.767
Yang Hu,Lei Liu,Li-peng Shan,Jiong Chen
White spot syndrome virus (WSSV) is an important pathogen causing high mortality in the shrimp industry in aquaculture, yet there is no treatment available to date. In order to find a treatment against WSSV infection, this study examined the anti-WSSV activity of eight natural compounds using shrimp larvae as a model. Among the eight compounds, paeoniflorin showed the most obvious anti-WSSV effect, with a maximum protection efficiency of WSSV-infected shrimp >60% at 100 μM. Furthermore, pretreatment and post-treatment experiments revealed that paeoniflorin could prevent and treat WSSV infection in shrimp. The antiviral activity of paeoniflorin in aquaculture water decreased rapidly with time, and the results showed that the stable anti-WSSV activity of paeoniflorin could only remain in water for 1 day. Thus, the dosing pattern of continuous medication changes was evaluated. Obviously, in the model of continuous change of paeoniflorin, WSSV copy numbers in the virus-treated shrimp group still progressively increased, while the virus content in WSSV paeoniflorin -treated group continued to decrease. Interestingly, paeoniflorin inhibited horizontal transmission of WSSV to a certain extent. Notably, paeoniflorin significantly increased the expression of antimicrobial peptides of shrimp to resist WSSV. In conclusion, paeoniflorin has the potential to protect shrimp against WSSV.
研究领域:
水产养殖
·
病毒学
·
海洋生物学
·
抗病毒研究
·
天然产物
✓ 已验证
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2026
·
IF 6.9
Qi Fang,Ashily Liang Wang,Yunzi Jiang,Weiqiang Qiu,Li Li,Yuhui Feng,Yingshan Jin,Chancong Jiang,Yinzhe Jin
This study investigated the mechanism of tropomyosin (TM) immunoreactivity reduction in Litopenaeus vannamei through ohmic heating (OH) combined with glycation. The screening of five common monosaccharides (glucose, xylose, ribose, galactose, and arabinose) and treatment times (0–120 min) revealed that galactose-treated TM samples subjected to OH (50 V/cm, 100 °C) for 60 min exhibited the lowest immunoreactivity. Following the OH–galactose treatment of purified TM and shrimp meat, the IgG/IgE binding capacity of purified TM decreased to 45.65% and 27.16%, respectively. The shrimp meat treatment group exhibited a further reduction in immunoreactivity to 39.99% and 25.75% due to the binding of endogenous sugars in meat with TM during treatment. Structural analysis revealed that there was a decrease in α-helix content, with a transition to β-sheet and random coil conformations, accompanied by reduced intrinsic fluorescence and increased surface hydrophobicity, indicating changes in the secondary and tertiary structures. Notably, the unique rapid volumetric heating characteristics of OH effectively drove these extensive structural modifications. After treatment, the content of amino acids decreased, accompanied by a reduction in the content of allergenic epitope peptides. Furthermore, the gastrointestinal digestibility of the sample was enhanced post-treatment, thereby facilitating human digestion and mitigating potential allergic risks. Molecular docking results demonstrated that galactose primarily binds to TM via glutamate-mediated interactions, which effectively masks the critical antigenic epitopes.
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DOI
高影响因子
危险品化学品经营许可证（带存储）