ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.6
Jing Gao,Feifei Ni,Yicun Chen,Ming Gao,Zhicheng Yu,Yunxiao Zhao,Yangdong Wang
As global attention to sustainable development continues to grow, plant dyes, as natural, renewable, and biodegradable biobased materials, are gradually becoming an ideal choice to replace traditional synthetic dyes due to their environmental friendliness, low toxicity, and sustainability. However, the application of plant dyes still faces challenges, including low extraction efficiency, poor dyeing performance, and the complexity and unclear nature of the dye components. This study presents a novel polyphenolic plant dye derived from the residues of Litsea cubeba fruits after essential oil extraction, and utilizes green deep eutectic solvent (DES) technology for efficient extraction and application. By screening and optimizing extraction conditions using response surface methodology, choline chloride/oxalic acid (ChCl/OA) DES was identified as the optimal solvent. Under conditions of 18% moisture content, a molar ratio of 0.75, and a solid-to-liquid ratio of 0.1, it significantly increased the polyphenol content in L. cubeba fruit residues. Compared to traditional extraction methods, the dye extracted using this method demonstrated significant advantages in both dyeing performance and antibacterial effects on silk. Furthermore, enzyme modification of the dye extracted with ChCl/OA successfully enhanced the color fastness of the fabric. More importantly, it was found that monomers such as p-hydroxycinnamic acid, trans-ferulic acid, and quercetin 3-β-d-glucoside play a key role in fabric dyeing performance, especially in enhancing dyeing strength and color fastness. This study provides theoretical support for the efficient extraction and application of polyphenolic plant dyes and promotes the sustainable development of green dyeing technologies.
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Chuyi Wang,Peipei Xu,Haonan Wang,Feili Luo,Qianmin Tu,Yulin Fang,Ruiyun You,Yan Yang,Yudong Lu
Early sex identification of the dioecious and medicinal spice plant, Litsea cubeba (Lour.) Pers (LC), is necessary for expanding the production and application of LC. We describe a Raman spectroscopy (RS) and surface-enhanced Raman spectroscopy (SERS) method combined with machine learning techniques. Based on the RS and SERS features, we combined principal component analysis and linear discriminant analysis (PCA-LDA) to dimension reduction for the data. And we combined seven machine learning algorithms, including logistic regression (LR), Naive Bayes (NB), decision tree (DT), random forest (RF), k-nearest neighbor (KNN), support vector machines (SVM), and extreme gradient boosting (XGBoost) algorithms to construct a gender prediction model for LC. The results demonstrated that the surface reinforcement treatment with drops of silver sol combined with LR, SVM, and XGBoost models achieved an accuracy of 84.62% in distinguishing male and female leaves. Furthermore, compared to the discrimination effect of scanning the leaf surface only, the gender recognition accuracy of the surface reinforcement treatment increased by 17.31%, 19.24% and 19.24%, respectively. Portable Raman spectroscopy combined with machine learning algorithms can be promoted for use as a tool for early sex identification in most plants, which can be applied to large-scale plant cultivation and breeding programmes.
✓ 已验证
产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2024
·
IF 8.7
Qiang Peng,Zhiwu Huang,Guixin Liang,Yongguang Bi,Fansheng Kong,Zhong Wang,Shaofan Tan,Junyong Zhang
Litsea cubeba essential oil (LCEO) has garnered widespread attention due to its robust biological activity. However, challenges such as high volatility, limited water solubility, and low bioavailability impede its application. Nano-emulsion encapsulation technology offers an effective solution to these issues. In this study, we prepared litsea cubeba essential oil nano-emulsion (LCEO-NE) for the first time using whey protein (WP) as the emulsifier through an ultrasonic-assisted method, achieving high efficiency with minimal energy consumption. Transmission electron microscopy and dynamic light scattering analyses revealed that the nanoparticles were uniformly spherical, with a particle size of 183.5 ± 1.19 nm and a zeta potential of −35.5 ± 0.95 mV. Stability studies revealed that LCEO-NE exhibited excellent thermal and salt stability, maintaining its integrity for up to four weeks when stored at 4 °C and 25 °C. In vitro digestion assays confirmed the digestibility of LCEO-NE. Furthermore, evaluation of the DPPH, ABTS, and antimicrobial activities revealed that LCEO-NE displayed superior bacteriostatic and antioxidant properties compared to LCEO. Scanning electron microscopy elucidated that its bacteriostatic effect involved the disruption of bacterial microstructure. Hemocompatibility and cytotoxicity assays demonstrated the safety of LCEO-NE within the effective concentration range. This research supports the utilization of nanoparticles for encapsulating LCEO, thereby enhancing its stability and bioactivity, and consequently expanding its applications in the food and pharmaceutical industries.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Ce Shi,Rui Yang,Yubin Zhao,Rongrong Cai,Tariq Aziz,Ashwag Shami,Fahad Al-Asmari,Fakhria A. Al-Joufi,Haiying Cui,Lin Lin
Protection of fragile blueberry from mechanical damage and fungal contamination through active packaging is essential for extending their shelf life. This study developed a dual-functional porous hydrogel from soy protein isolate (SPI) and sodium alginate (SA) incorporated with Litsea cubeba essential oil nanoemulsions (LCEONs) for blueberries postharvest preservation. Results demonstrated that the prepared LCEON-4% was chosen for hydrogel incorporation due to optimal droplet size, uniform distribution, stability and encapsulation efficiency, and the resulting SPI/SA-LCEON composite porous hydrogels showed tight networks, outstanding cushioning and mechanical performance, as well as high water retention and swelling rate. Additionally, the composite porous hydrogels also exhibited efficient antioxidant activity and antifungal properties against Botrytis cinerea . Results in the practical application test found that the SPI/SA-LCEON composite porous hydrogels significantly reduced the weight loss and decay rate while effectively preserved fruit firmness, color, and key quality attributes (titratable acidity, soluble solids, phenols, and flavonoids) of the blueberries at 4 °C for 9 days. These findings here demonstrated the great potential of the dual-functional composite porous hydrogels as a promising sustainable material for the preservation of high-value and fragile fruits.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2023
·
IF 2.7
Jiaoli Huang,Sisi Liu,Rukuan Liu,Youjin Yi,Changzhu Li,Zhihong Xiao,Jia Tu,Jingjing Xiao
Although Litsea cubeba essential oil (LCEO) is widely used for against fungi, antifungal mechanism of it is not fully understood. In the study, antifungal potential and mechanism of LCEO on Colletotrichum scovillei , the main pathogen fungi caused pepper anthracnose , were studied. Results of gas chromatograph-mass spectrometry (GC-MS) showed that citral (67.37%) and d -Limonene (11.72%) were the major components of LCEO. LCEO was effective in inhibiting germination of C. scovillei spores , and the minimum inhibitory concentration (MIC) was 0.60 mg mL −1 . To determine influence of LCEO on cell membrane integrity and permeability of C . scovillei , SEM observation, OD 260 nm (nucleic acids), OD 280 nm (proteins), extracellular electric conductivity , cell membrane permeability (propidium iodide, PI), cell wall integrity (calcofluor white, CFW), malondialdehyde (MDA) content and ergosterol content were analyzed. Spores exposed to LCEO were partly deformed, collapsed, shrunken and shriveled. The OD 260 nm, OD 280 nm and the extracellular electric conductivity of LCEO treatment group increased by 2.0, 1.5 and 1.2 times, respectively, at 12 h after treatment of LCEO (0.6 mg mL −1 ). Besides, high accumulation of MDA content indicated that LCEO significantly enhanced membrane lipid peroxidation . Moreover, LCEO induced a decrease in ergosterol content of C. scovillei . These findings suggest that LCEO exhibited potent inhibitory activities against C. scovillei , and the cell membrane permeability and cell wall integrity of C. scovillei treated by LCEO were significantly destroyed.
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Xiaoci Zhuang,Zhen Zhang,Yong Wang,Ying Li
Litsea cubeba (LC) is an indigenous industrial crop in China with fruits possessing interesting essential oil content of 3-5%, which production leads to a large number of kernels as wastes. In this study, the performance of four alternative solvents on the extraction of LC kernel oils was firstly evaluated in comparison with n-hexane as the reference solvent. Regardless of the solvent used, all extracted oils were quantitatively and qualitatively analyzed, where green solvents (dimethyl carbonate-DMC and cyclopentyl methyl ether-CPME) were preferred to alcoholic solvents (ethanol and isopropanol) considering their higher extraction yields and similar acylglycerol and fatty acid compositions to oils extracted by n-hexane. Interestingly, micronutrients in oils extracted by green solvents were quantified much higher than that extracted by n-hexane. Apart from common physicochemical properties measured, Hansen solubility parameter and antioxidant activity in vitro were also studied to make a more comprehensive profile for solvent effects and oil functionality. The results indicated that CPME could be the optimal alternative solvent to n-hexane with comparable extractability and selectivity in the green extraction of LC kernel oils as a new promising natural plant oil resource.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Amer Ali Mahdi,Qais Ali Al-Maqtari,Waleed Al-Ansi,Wei Hu,Sulafa B.H. Hashim,Haiying Cui,Lin Lin
This study evaluated the effects of adding various concentrations (0 %, 1 %, 2 %, and 3 %) of peach gum (PG) to films made from polyethylene oxide (PEO) combined with Litsea cubeba essential oil (LCEO) to be utilized as active packaging for food in the future. The findings showed that the film containing PG 2 % concentration had the best physic-mechanical properties. In films made with PG, the glass transition temperature was significantly improved. Combining PG and PEO resulted in films that were brighter in color, had lower WVP values, and had the lowest water activity. Furthermore, XRD demonstrated that PG additions were compatible with the film of PEO blended with LCEO. The PG films formulated with PG presented high antioxidant and antibacterial activity against Staphylococcus aureus and E. coli . Wrapping beef with P2G2 film led to maintaining its quality with suitable levels of pH, TBARS , and TVB-N. This also decreased the number of E. coli and S. aureus in beef throughout the storage period. The results indicate that adding PG to PEO films enhances their suitability for food preservation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Yu Guo,Yan Li,Zhuang Li,Li Jiang,Xifeng Cao,Wenbin Gao,Jun Wang,Duqiang Luo,Fengli Chen
As an important natural product, the sufficient separation of plant essential oil (EO) is helpful to improve its utilization value. In this work, deep eutectic solvent-homogenate based microwave-assisted hydrodistillation (DES-HMAHD) was developed and applied to isolate EO from the fruits of Litsea cubeba (Lour.) Pers. Different types of DES were investigated in terms of the EO kinetics and composition, among which oxalic acid/choline chloride (OA/ChCl) had obvious advantages. Following, molar ratio of OA and ChCl (1:1), water content (50%), liquid-solid ratio (12.5:1 mL/g), homogenate time (2 min), and microwave power (700 W) were found to be the optimum conditions. Gas chromatography-mass spectrometer (GC-MS) analysis showed that the EO isolated from DES-HMAHD contained a large proportion of m -cymene and trans -linalool oxide, which were quite different from the conventionally reported L. cubeba EO. In addition, the proposed DES-HMAHD resulted in higher separation efficiency and economic value, as well as lower environmental impact, as compared with other techniques. Afterwards, the EO isolated by different methods was evaluated from the perspective of biological activity. The EO obtained by DES-HMAHD showed higher antioxidant activity (DPPH and ABTS) but lower antifungal activity, which was related to its chemical composition. In general, DES-HMAHD produced a kind of L. cubeba EO with different components, which provided a scientific foundation for the sufficient isolation of plant EO and its application in the natural products.
✓ 已验证
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Guorong Fan,Xiaodan Ning,Shangxing Chen,Liang Zhong,Chunce Guo,Yuling Yang,Juan Liu,Ming Tang,Guoxiang Liao,Xuefang Wang,Zongde Wang,Jiawei Wang
Litsea cubeba (Lour.) Pers. is an important industrial tree in southern China and Southeast Asia. We surveyed and sampled wild L. cubeba in 32 provenances spread across nine provinces of China. We used nested variance analysis, multiple comparisons analysis, hierarchical clustering analysis, Pearson’s correlation analysis, path analysis with multiple stepwise regression, detrended correspondence analysis, redundancy analysis, and variance partitioning analysis to evaluate the phenotypic diversity of fresh fruit yield (FFY) and essential oil (EO) characteristics of L. cubeba , and to explore the relationship between the main habitat factors and phenotypic diversity. We detected wide phenotypic diversity in FFY, EO content (EOC), citral yield (CitrY), and the proportions of the seven main components of EOs (citral, D -limonene, 3,7-dimethyl-3,6-octadienal, 4-methyl-3-pentenal, linalool , citronellal , and 1-(cyclopanecarbonyl)piperidin-4-one). The variation was mainly derived from among provenances ( P ≤ 0.01) rather than within provenances, and the variation in FFY and CitrY was greater than that of EOC and the seven dominant components among provenances. We also detected several region-specific components of EOs in fresh fruit, including α-terpineol, geraniol , eucalyptol , and 7-methyl-3-methyleneoct-6-enal. Most of the provenances with higher FFY were distributed in southwestern China (lower longitude and latitude, and higher elevation), while most of those with higher EOC were distributed in southeastern China (higher longitude and latitude, and lower elevation). The three provenances with excellent FFY were YN-XC, GZ-ZY, and SC-KJ; the two with higher EOC were SC-KJ and HN-DX; and CitrP was higher in GZ-ZF than in other provenances. Longitude, total potassium content (TotalKC), and bulk density (BD) were the most important factors that influenced FFY diversity, while latitude, elevation, annual average relative humidity (AARH), and organic carbon content (OCC) most strongly influenced EOC diversity, and annual average temperature and annual average maximum temperature (AAMaxT) influenced CitrP diversity. The six main habitat factors together explaining the largest proportions of phenotypic diversity were longitude, AARH, AAMaxT, elevation, latitude, and soil total phosphorus content (TotalPC). Higher soil TotalKC, BD, and TotalPC were suitable conditions for cultivation of L. cubeba to achieve higher FFY, while lower AARH and OCC were favorable for accumulation of EOs, and only lower temperature was more likely conducive to citral synthesis. The wide diversity of FFY and EO characteristics should be taken into account when selecting germplasms for protection and utilization, and the identification of the most important environmental factors affecting phenotypic diversity provides a theoretical basis for the optimization of L. cubeba cultivation.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xuewen Ni,Weilu Xiao,Xiaohan Liu,Shuangyang Peng,Yuehan Wu,Zhiming Gao
With increasing demands for environmental protection and food safety, biodegradable active food packaging is gaining attention. Hereby, konjac glucomannan/carrageenan blend films (KCNTO) incorporating Litsea cubeba essential oil (LCEO) Pickering emulsion stabilized by cellulose nanocrystals/tannic acid were developed and evaluated for their preservation effect on pork. The formation, structure, physical properties, antioxidant and antimicrobial activities of KCNTO, as well as the release of LCEO were investigated. The results showed that the viscosity and pseudoplasticity of KCNTO film-forming solutions decreased due to LCEO Pickering emulsion interfering with the molecular entanglement network of konjac glucomannan/carrageenan. LCEO droplets were homogeneously distributed in KCNTO to make its structure loose. Hydrogen bonding interactions occurred between LCEO Pickering emulsion and the film matrix. The addition of LCEO Pickering emulsion improved KCNTO's elongation at break, water contact angle, water vapor barrier and UV shielding, while giving it excellent antioxidant and antimicrobial activities. LCEO was slowly and sustainably released from KCNTO at 25 °C in air. KCNTO-8 maintained the quality of pork by slowing down the increase in pH, aerobic plate count and thiobarbituric acid reactive substances during storage at 4 °C. The findings suggested that KCNTO was a promising active packaging material for pork preservation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 10
·1707
·
IF 5.1
Li Li,Ying Hu,Yu-Mei Wang,Xiao-Xue Wu,Si-Tong Lin,Hang Li,Ji Zhang,Guo-Rong Fan,Zong-De Wang,Bin Wang,Shang-Xing Chen
In this study, we systematically investigated the mechanisms of the antioxidation and anti-lipid accumulation effects of antioxidant peptides fromLitsea cubebaon a free fatty acid (FFA)-induced NAFLD model of HepG2 cells. The NAFLD cell model was constructed by inducing the HepG2 hepatocellular carcinoma cell line with 0.5 mmol/L FFAs, and AQRDAGLL, QEGPFVR, and DVPPPRGPL were given to the culture to study their lipid-lowering and antioxidant activities on NAFLD cells. The lipid-lowering activities of the three antioxidant peptides were evaluated by Oil Red O staining and TG and TC content assays, and the results showed that all three peptides had strong ameliorating effects on FFA-induced lipid accumulation in NAFLD cells. The intracellular antioxidant protease (CAT, GSH, and SOD) activity levels and lipid peroxidation (MDA) content were measured and intracellular ROS levels were detected. The results showed that after intervention with the antioxidant peptides, the intracellular ROS levels in the NAFLD model cells were significantly reduced, the SOD and CAT activities were increased, the GSH content was elevated, and the MDA content was reduced, which indicated that AQRDAGLL, QEGPFVR, and DVPPPRGPL were able to inhibit the oxidative stress of the cells effectively and to achieve the effect of intervening in NAFLD. JC-1 fluorescence staining experiments showed that the mitochondrial membrane potential function of NAFLD cells was restored under the effect of the antioxidant peptides. Molecular dynamics simulations revealed that the main driving force between QEGPFVR and Keap1 protein was van der Waals forces, ΔG = −62.11 kcal/mol, which indicated that QEGPFVR was capable of spontaneously binding to Keap1 protein.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）