Food Frontiers
·
2024
·
IF 7.4
Lu An,Wenbo Wang,Jihao Chu,Lin An,Bingyu Geng,Xueqi Fu,Jing Chen,Junfeng Ma
Alzheimer's disease (AD) poses a significant global health challenge, primarily characterized by the aggregation of β-amyloid (Aβ) peptides and associated oxidative stress. Given the lack of effective AD drugs, we investigated the effects of Lycium barbarum polysaccharide (LBP), a water-soluble polysaccharide from goji berries, on Aβ scavenging, aggregation, and Aβ-induced toxicity using the transgenic AD nematode. LBP treatment delayed paralysis, mitigated Aβ-induced chemotaxis deficits, and reduced Aβ 1–42 deposition and oligomer formation in AD nematodes. Furthermore, LBP supplementation alleviated Aβ-induced oxidative stress and improved oxidative stress resistance through the insulin/insulin growth factor 1 (IGF-1) signaling (IIS) pathway. Evaluation of the Aβ clearance pathway revealed that LBP attenuated Aβ toxicity by increasing proteasome activity rather than lysosomal levels. Basic physiological assessments further indicated that LBP was non-toxic in vivo and promoted nematode health. Our findings suggest that LBP ameliorates AD-like symptoms in nematodes by activating the IIS pathway to reduce oxidative stress and enhancing proteasomal activity to clear Aβ aggregates, providing a new theoretical basis for considering LBP as a promising therapeutic drug for AD and related oxidative stress complications.
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Journal of Functional Foods
·
2026
·
IF 3.9
Rongrong Sun,Yanghanxiu Liu,Mengyao Xia,Yihui Yang,Hao Meng,Qing Hu,Xichun Peng,Liu Liu,Jianming Luo
Our previous studies have shown that Lycium barbarum polysaccharide (LBP) alleviates rheumatoid arthritis (RA) symptoms in gut microbiota-dependent manner, with the key bacterium and the underlying mechanism remain unidentified. In this study, LBP was fermented in vitro by Romboutsia lituseburensis , Lactobacillus johnsonii , or a combination of both strains, and were administered to pseudo-germ-free RA rats. Only R. lituseburensis- fermented LBP significantly alleviated RA and markedly enriched osteoclast differentiation pathway-related genes. Twenty-six metabolites produced by R. lituseburensis , including glutamic acid-proline-glutamine (EPQ), that reduced inflammation and inhibited osteoclast differentiation by downregulating Rankl expression and its downstream factors. Notably, EPQ was also proposed to bind RANKL and potentially interfere with the RANKL/RANK pathway, thereby suppressing the expression of osteoclastogenesis-related genes. These findings identify R. lituseburensis as the key bacterium mediating the anti-RA effects of LBP, with its fermentation products, particularly EPQ, serving as critical active substances that inhibit inflammation and modulate osteoclast differentiation via the RANKL-RANK signaling.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yi-Long Ma,Xin Guo,Qian-Lan Wu,Jing Li,Tian-Le Mao,Zhi Li,Kiran Thakur,Zhao-Jun Wei
Chlorogenic acid (CGA), a phenolic compound with extensive bioactive properties, holds significant potential for various applications. However, its practical use has been limited by its poor thermal stability, oxidative sensitivity, and low bioaccessibility. To overcome these challenges, we developed zein and Lycium barbarum polysaccharide (LBP) encapsulated CGA nanoparticles (ZLC NPs), optimized through Box-Behnken Design. The optimized ZLC NPs exhibited exceptional physicochemical properties, with an average particle size of 122.7 ± 0.25 nm, a PDI of 0.070, and an encapsulation efficiency of 85.66 %. Under thermal treatment, the CGA of ZLC NPs retained 95.31 % after 90 °C for 3 h, significantly surpassing the recovery rate of free CGA (81.65 %). During the simulated digestion, the ZLC NPs displayed improved bioaccessibility (41.3 %) compared to free CGA (28.4 %). The ZLC NPs also showed higher radical scavenging rates (68.0–72.7 %), outperforming free CGA (36.5 %–51.4 %). At a concentration of 30 μg/mL, ZLC NPs increased AML-12 cell viability to 69.56 %, compared to 50.08 % for free CGA. These results indicate that ZLC NPs effectively overcome the limitations of free CGA, offering enhanced stability, bioaccessibility, antioxidant activity, and cellular protection. These advanced properties position ZLC NPs as promising candidates for incorporation into functional foods and pharmaceutical formulations.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Guoyan Liu,Xue Yang,Jixian Zhang,Li Liang,Feng Miao,Tao Ji,Zhiqiang Ye,Meng Chu,Jiaoyan Ren,Xin Xu
Lycium barbarum polysaccharides (LBP) with different molecular weights (LBP1, LBP2 and LBP3) of 92,441 Da, 7714 Da, and 3188 Da were used as stabilizers and capping agents to prepare uniformly dispersed selenium nanoparticles (SeNPs), and determined the storage stability. In addition, the anti-fatigue activity of LBP-decorated SeNPs with the best stability (LBP1-SeNPs) was estimated by using forced swimming test . The results showed that LBP1-SeNPs exhibited smaller particle size and more excellent stability than those of LBP2-SeNPs and LBP3-SeNPs when the storage time was extended to 30 days, and the average particle size was maintained at about 105.4 nm. The exhaustion swimming time of all tested dose groups of LBP1-SeNPs was significantly longer than the control group ( p < 0.05), and the high-dose group among them was even obviously longer than the positive group ( p < 0.05). The results of glycogen, blood urea nitrogen (BUN), blood lactic acid (BLA), superoxide dismutase (SOD), and malondialdehyde (MDA) levels were further confirmed that LBP1-SeNPs could relieve fatigue by increasing the reserve of glycogen, enhancing antioxidant enzyme levels and regulating metabolic mechanism. These results demonstrated that LBP1-SeNPs could be developed as a potential anti-fatigue nutritional supplement.
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CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Renwu Zhou,Rusen Zhou,Feng Yu,Dengke Xi,Peiyu Wang,Jiangwei Li,Xingquan Wang,Xianhui Zhang,Kateryna Bazaka,Kostya (Ken) Ostrikov
Pesticides are widely used in commercial goji Lycium barbarum cultivation to prevent damage of the delicate fruits by insects, potentially leading to insecticide and fungicide residues at levels dangerous to human health. In this study, removal of organophosphorus pesticide (OP) residues (i.e. omethoate and dichlorvos) from the surface of L. barbarum was conducted using a gas phase surface discharge (GPSD) plasma system. ICCD and GC–MS measurements showed that a homogeneous and uniform GPSD glow-like plasma could be achieved and the efficiency of OP residues degradation greatly depended on the applied voltage and plasma exposure time, with 99.55% and 96.83% efficiency recorded for omethoate and dichlorvos (DDVP), respectively after 30 min GPSD plasma exposure at the discharge voltage of 10 kV. FT-IR and chemical analysis of the intermediates produced during GPSD exposure revealed that the omethoate and DDVP molecules could be completely degraded into nontoxic species, at the appropriate dosage of GPSD plasma exposure, without compromising the quality of L. barbarum . Based on the experimental and simulation results, a mechanism of OP degradation attributed to energetic electrons and highly reactive species generated by GPSD plasma was proposed.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ying Wang,Feng Bai,Qiang Luo,Mingliang Wu,Gang Song,Hongmei Zhang,Jian Cao,Yanqing Wang
Lycium barbarum polysaccharide-doxorubicin (LBP-DOX) nanoparticles were synthesized via a one-step Schiff base reaction between oxidized Lycium barbarum polysaccharide (oxidized LBP) and doxorubicin (DOX). The structure of LBP-DOX nanoparticles was characterized by multi-spectral methods, 1 H nuclear magnetic resonance ( 1 H NMR), transmission electron microscopy (TEM), and thermo gravimetric analysis (TGA). The rheological properties of LBP-DOX nanoparticles were studied. With the increase of shear rate, the viscosity of LBP and LBP-DOX nanoparticles gradually decreases at 25 °C. Under the same strain, the storage modulus (G′) and loss modulus (G″) of LBP-DOX nanoparticles were inferior to LBP. The DOX release from LBP-DOX nanoparticles was pH-dependent and was accelerated by decreasing pH. Cytotoxicity study showed that the LBP-DOX nanoparticles have significantly enhanced cytotoxicity in vitro, especially for human Hepatic cancer cell line HepG2.
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SCIENTIA HORTICULTURAE
·
2024
·
IF 3.9
Xiaojie Pei,Shuxin Sun,Xin Dai,Ke Liu,Ying Yang,Laifeng Lu,Yansheng Wang
Goji berry ( Lycium barbarum ) is a typical berry with a tender and thin pericarp, high moisture content and hollow interior, making it highly susceptible to mechanical damage caused by harvest and transportation. As a consequence, this susceptibility results in considerable economic losses within the fresh produce industry due to the degradation of fruit quality and subsequent decay. Here, oligogalacturonides (OGs), a well-characterized plant damage-associated molecular pattern, were applied to reduce postharvest loss and extend the shelf life of berries of the goji cultivar NingQi1. The loss rate in the OGs treatment was 44.45 % lower than that in the 0.8 mm compression treatments on Day 6, and the OGs treatment also effectively inhibited softening and delayed weight loss. The activity of superoxide dismutase increased by 27.4 % on the 4th day after OGs treatment, accompanied by a decrease in malondialdehyde content ranging from 14.2 % to 24.8 % on days 2 to 4 compared to the control group. Furthermore, the phenylalanine ammonia-lyase activity of OGs-treated goji berries was 57.0 % higher than that of the control at 6th days, which slowed the reduction in secondary metabolites such as total flavonoids. The findings of this study indicated that OGs may reduce postharvest losses caused by mechanical damage, extend the shelf life of goji berry by reducing softening and weight loss, and reduce wounding injury by minimizing free radical form of reactive oxygen species.
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Natural Products and Bioprospecting
·
2025
·Vol. 15
·期 1
·1-8
·
IF 4.9
Wang Zi-Jiao,Tan Bang-Yin,Zhao Yun,Wang Chang-Bin,Zhao Yun-Li,Luo Xiao-Dong
Two new together with 32 known compounds were isolated from the leaves of Lycium barbarum . Their structures were elucidated using 1D and 2D NMR, HRESIMS, and ECD spectroscopic techniques. Compounds 1–34 were evaluated for their anti-rheumatoid arthritis activities in a lipopolysaccharide (LPS)-induced MH7A cells inflammatory model. As a result, compounds 1– 3 , 6 , 8 , 10 , 14 , 17–19 , 29 and 31 inhibited the activity of lactate dehydrogenase (LDH) and nitric oxide (NO) at concentrations 20 μM. Among them, compound 1 showed the best effectiveness, with inhibition rates of 46.7% for NO and 32.8% for LDH. Graphical
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Natural Product Communications
·
2026
·
IF 1.5
Wen Zhang,Yunuo Ren,Xiaoxi Liu,Xingmiao Liu,Weiwei Tang,Yingjie Ju,Wei Yu,Zhimou Guo,Huanhuan Qiao,Xiaoli Yu,Suxia Ren,Tianxiang Li,Yuqing Dong,Zengyi Huang,Youxin Li,Zhiguang Yuchi,Dong Li,Yiwen Wang
Background/ObjectivesLycium barbarum L. (Gouqi, GQ) is a prominent component of traditional Chinese medicine with well-established medicine-food homology properties. Owing to its high nutritional profile, GQ is widely recognized for its diverse pharmacological benefits, including protective effects on the kidney. The primary objective of this study was to evaluate the anti-urolithiatic activity of GQ aqueous extract and to elucidate its underlying therapeutic mechanisms in the context of calcium oxalate (CaOx) nephrolithiasis (Kidney stone).MethodsThe anti-kidney stone activity of the GQ extract was assessed in a Drosophila melanogaster (fruit fly) model of CaOx kidney stones induced by dietary sodium oxalate (NaOx). Oxidative stress levels were quantified via dihydroethidium (DHE) staining. The expression profiles of key enzymes involved in endogenous oxalate synthesis—namely, agxt (the ortholog of human alanine-glyoxylate transaminase, AGT) and daao1/daao2 (encoding two D-amino acid oxidases, DAOs)—were analyzed using RT-qPCR technology. Excretory function was evaluated by counting fly fecal deposits. In vitro crystallization assays were performed to examine evolution of CaOx crystal morphology and explore the regulatory activity of the GQ extract during crystal formation.ResultsOur results demonstrated that the GQ extract effectively attenuated CaOx kidney stone formation through multiple interconnected mechanisms. Specifically, GQ extract mitigated NaOx-induced oxidative stress, improved urinary excretion, reduced endogenous oxalate biosynthesis, and directly altered the morphological dynamics of CaOx crystallization.ConclusionsThese findings substantiate the significant stone-preventive potential of GQ, whose therapeutic effects are mediated via a pleiotropic mode of action. Collectively, our data suggest that GQ may serve as a valuable dietary supplement or potential therapeutic agent for the prevention and management of CaOx kidney stones.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2026
·
IF 3.5
Guo Xiumei,Liu Peng,Zhang Minsong,Song Meijia,Shi Manman,Gao Hongzeng,Cui Jiandong
The traditional extraction method of Lycium barbarum pigment has some problems, such as long extraction time, large solvent consumption, low extraction efficiency and serious environmental pollution, so it is necessary to develop a new extraction method with shorter treatment time, lower cost, easier operation and environmental friendliness than the traditional method. In this study, the pigment from Ningxia Lycium barbarum was extracted, purified and identified by deep eutectic solvents assisted by surfactant. The pigments from Lycium barbarum from Ningxia were extracted, purified and identified by surfactant-assisted deep eutectic solvents (DES). The maximal pigments extraction rate was obtained under optimal conditions (3% surfactant Tween 20 (v/v), menthol-lactic acid with water content of 50% (molar ratio of 1:4), 1% material-liquid ratio (m/v), the water bath at 30 ℃ for 2.5 h). The pigments extraction rate by surfactant-assisted DES was 6-folds higher than traditional extraction agents (ethanol), and reached 32.09 mg/g. The HPLC detection results showed that the main component of the pigments was corn lutein dipalmitate with 348.861 mg/g. Furthermore, the purified pigments exhibited excellent free radical scavenging activities for DPPH and ABTS + , and ·OH radicals, indicating its high antioxidant activity.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Jixian Zhang,Tao Ji,Xue Yang,Guoyan Liu,Li Liang,Xiaofang Liu,Chaoting Wen,Zhiqiang Ye,Maowei Wu,Xin Xu
Selenium nanoparticles (SeNPs) in an aqueous solution have poor stability and tend to aggregate when stored for a long time. In the present study, SeNPs were stabilized by using Lycium barbarum polysaccharide (LBP) and Lycium barbarum protein (LBPr) conjugates (LBPP) as a stabilizer and dispersing agent. Particularly, the LBPP 1 was obtained with subcritical water treatment. In addition, the physical stability, re-dispersity and antitumor activity of LBPP 1 -SeNPs were investigated. The results showed the particle size of LBPP 1 -SeNPs was maintained at 111.5–117 nm, which was stable at PH 6, 4 °C and darkness for at least 40 days. Besides, the result of TEM showed that the dispersion of LBPP 1 -SeNPs had more clear layers and smoother surfaces. Moreover, LBPP 1 -SeNPs had excellent re-dispersibility and exhibited a significant inhibitory effect on HepG-2 cells and Caco-2 cells, respectively ( p < 0.05). Therefore, LBPP 1 -SeNPs can be used as potential selenium nutritional supplements for food and medical applications.
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MOLECULES
·
2020
·Vol. 25
·期 6
·1351
·
IF 3.267
Le Feng,Xiao Xiao,Jing Liu,Junyan Wang,Nan Zhang,Tao Bing,Xiangjun Liu,Ziping Zhang,Dihua Shangguan
Lycium barbarumL. is a widely used functional food and medicinal herb in Asian countries.L. barbariumpolysaccharides (LBP) are considered as one of the major medicinal components ofL. barbariumfruit and exhibits a wide range of biological activities. Here, we investigated the immunomodulatory effects of LBP and its uptake behaviors at the cellular level. LBP was prepared by water extraction and ethanol precipitation, and divided into two fractions based on the molecular weight distribution by ultrafiltration (LBP > 10 kDa and LBP < 10 kDa). The physicochemical properties of LBP and LBP fractions were well characterized. The LBP > 10 kDa fraction greatly enhanced the viability of macrophages RAW264.7 cells and induced cell polarization, but had weak effects to other tested tumor cell lines and normal cell line. This fraction could regulate the production of NO, TNF-α, IL-6 and ROS in RAW264.7 cells, suggesting both pro-inflammatory and anti-inflammatory effects. The dye-labeled LBP could be internalized into all tested cell lines and accumulated in lysosomes. The internalization of LBP in RAW264.7 cells is mainly through the clathrin-mediated endocytosis pathway. The Caco-2 intestinal transport experiment demonstrated that the dye labeled LBP could be transported through the Caco-2 cell monolayer (mimic intestinal epithelium) through clathrin-mediated endocytosis. These results demonstrate the immunomodulatory effects of LBP and its effective uptake by macrophages and intestine.Keywords:L. barbariumpolysaccharide;immunomodulatory effects;polysaccharide uptake;extraction;physicochemical property
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危险品化学品经营许可证（带存储）