CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Shihai Zhang,Fei He,Xia Chen,Kan Ding
Crude polysaccharides were obtained from fruits of Lycium ruthenicum Murr using hot water extraction followed by ethanol precipitation. A homogeneous polysaccharide, LRP3-S1 with a relative molecular weight of 114.8 kDa was purified by anion-exchange chromatography on DEAE Sepharose ™ Fast Flow and Sephacryl S-300 HR column. Monosaccharide composition analysis revealed that LRP3-S1 was composed of rhamnose, galacturonic acid, galactose, xylose and arabinose in a molar ratio of 14.4: 17.7: 26.6: 16.4: 24.9. LRP3-S1 contained a rhamnogalacturonan I (RG-I) backbone partially substituted at C-4 of rhamnose units by side chains, which included T-linked β-D-Gal p , 1,3-linked β-D-Gal p , 1,6-linked β-D-Gal p , 1,3,6-linked β-D-Gal p , 1,5-linked α-L-Ara f , 1,3,5-linked α-L-Ara f , T-linked α-L-Ara f and T-linked β-D-Xyl p . Biological activity tests showed that LRP3-S1 could inhibit the growth of pancreatic cancer cells. In addition, LRP3-S1 could attenuate invasion ability of BxPC-3 cells and down-regulate protein expression of p-FAK, p-AKT, p-GSK-3β and p-p38 MAP kinase.
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Jingrong Liu,Hualin Wang,Min Guo,Linlin Li,Minmin Chen,Suwei Jiang,Xingjiang Li,Shaotong Jiang
This study was to design a novel wide pH–sensing colorimetric film of κ-carrageenan incorporated with extract from Lycium ruthenicum Murr. (Car-LRM) for food freshness monitoring. The structure, basic properties (thermal stability, mechanical, barrier and antioxidant activity), and release kinetics of LRM from the films were investigated. Attentions were centered on colorimetric response of films and their applications for freshness monitoring of milk and shrimps. A low incorporation of LRM (no more than 2.5%) led to an enhancement in thermal stability and water vapor barrier of films to a certain extent. At above level of incorporation, LRM could be well-distributed in Car matrix by the interactions of hydrogen bonds. In addition to good antioxidant activity , the film displayed colorimetric response at a wide range of pH (2−10) and the color change was reversible. The release of LRM from films followed Fickian's law which was controlled by diffusion mechanism rather than swelling mechanism. The successful applications of Car-LRM2.5 for the freshness monitoring of milk and shrimps indicate that Car-LRM2.5 may have great potential for monitoring the freshness of milk and aquatic products.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ying Li,Yu Zhang,Li Zhang,Jun Dang
The screening of enzyme inhibitors faces challenges such as low efficiency. In this study, a novel online HPLC-FLD/pancreatic lipase recognition system was developed for the first time. The optimal operating parameters were established as a flow rate of 1 mL/min, with excitation and emission wavelengths for pancreatic lipase set at 221 nm and 303 nm, respectively. The effectiveness of this system was validated using orlistat. Subsequently, this system was applied to Lycium ruthenicum , where two petunidin-based anthocyanins pancreatic lipase inhibitors were efficiently isolated using medium-pressure liquid chromatography. Molecular docking elucidated that compounds 1 and 2 interact with the serine residue of pancreatic lipase, exhibiting binding energies of −13.12 kcal/mol and −12.22 kcal/mol, respectively. Enzymatic assays showed that the half-maximal inhibitory concentrations of compounds 1 and 2 were 9.67 μM and 147.37 μM, respectively. These docking and inhibition results confirm the system for efficiently discovering pancreatic lipase inhibitors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yadan Zhang,Xiaoyu Zhang,Tianhan Kai,Lin Zhang,Anping Li
Plant-derived exosome-like nanovesicles (ELNs) are nano-sized vesicles extracted from edible plants. Lycium ruthenicum Murray (LRM) has been gaining increasing attention due to its nutritional and medicinal value, but the ELNs in LRM has not been reported. In this study, LRM-ELNs were obtained, and the proteins, lipids, microRNAs (miRNAs) and active components in LRM tissues and LRM-ELNs was analyzed by LC-MS/MS, LC-MS, high-throughput sequencing techniques, and physical and chemical analysis. LRM-ELNs can be uptaken by PC12 cells through macropinocytosis and caveolin-mediated endocytosis primarily. Transcriptomic and western blot experiments indicate that LRM-ELNs can inhibit Aβ-induced apoptosis in PC12 cells through the MAPK and PI3K/AKT signaling pathways, with miRNAs playing a crucial role. These results indicate that LRM-ELNs have the protection effect on PC12 cells and can be considered as dietary supplements for alleviating neurodegenerative diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Quality and Safety
·
2020
·Vol. 4
·期 4
·193-201
·
IF 0
Liu Bei,Xu Qingqing,Sun Yujing
Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects of water temperature and soak time on the colour, phytochemicals, and the antioxidant capacity [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the ferric-reducing antioxidant power (FRAP)] of two goji berry tea. A comparison of the bioactive compounds and antioxidant activities between black and red goji berry tea was conducted. Results showed that both red and black goji berry tea were rich in phytochemicals, giving high antioxidant ability. The levels of bioactive compounds and the antioxidant activity of the two goji berry tea increased as the increases in soak temperature and time. Black goji berry tea had higher phytochemicals and antioxidant property than those of red goji berry tea. Infused at 100° water for the same time, the levels of total polysaccharides (150 mg/100 ml), total polyphenols (238 mg/ml), and antioxidant capacity (550 μmol/100 ml) of black goji berry tea were 3.5, 2, and 5 times higher, respectively, in comparison with red goji berry tea. The results of this study demonstrate that hot drink of goji berry in China is a good habit and black goji berry tea may be a better choice.
✓ 已验证
产品关联
DOI
INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Yao Zhao,Chong Ma,Ming Qiao,Qiang Hou,Junping Hu,Jianhua Yang
Optimizing the extraction and valorization of Lycium ruthenicum Murr. polysaccharides for high-value applications are critical for its application in bio-based industries. To address the limitations of conventional processing, this study developed and optimized a novel integrated strategy combining enzyme-assisted extraction (EAE), ultrasound-assisted extraction (UAE), and aqueous two-phase system (ATPS). Response surface methodology was employed to determine the optimal extraction parameters by investigating the interactive effects of cellulase and pectinase dosages, along with phase composition. Subsequently, the physicochemical properties of the purified Lycium ruthenicum polysaccharides (LRPs) were characterized, and their anti-aging mechanisms were evaluated in a D -galactose-induced aging mouse model through histopathological analysis, biochemical assays, and 16S rRNA sequencing of gut microbiota. Under the optimized extraction conditions, LRPs were efficiently obtained with a high yield of 35.25% and a total sugar content of 93.58%. Structural analysis identified LRPs as acidic heteropolysaccharides (molecular weight ∼55 kDa) rich in uronic acids (11.13%), composed primarily of glucose, galactose, and arabinose. In vivo experiments demonstrated that LRPs significantly attenuated systemic oxidative stress and inflammation, restoring hepatic and retinal architecture. This protective effect was linked to increased levels of antioxidant enzymes, including superoxide dismutase (SOD), and reduced pro-inflammatory cytokines, such as tumor necrosis factor- α (TNF- α ).Moreover, LRPs beneficially restructured the gut microbiota by enriching Akkermansia and suppressing Romboutsia. In conclusion, the integrated green extraction strategy efficiently yields bioactive LRPs that mitigate hepatic and retinal aging, potentially via the regulation of the gut-liver-retina axis.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Chu Zhang,Wenyan Wu,Lei Zhou,Huan Cheng,Xingqian Ye,Yong He
Black goji berry ( Lycium ruthenicum Murr.) has great commercial and nutritional values. Near-infrared hyperspectral imaging (NIR-HSI) was used to determine total phenolics, total flavonoids and total anthocyanins in dry black goji berries. Convolutional neural networks (CNN) were designed and developed to predict the chemical compositions. These CNN models and deep autoencoder were used as supervised and unsupervised feature extraction methods, respectively. Partial least squares (PLS) and least-squares support vector machine (LS-SVM) as modelling methods, successive projections algorithm and competitive adaptive reweighted sampling (CARS) as wavelength selection methods, and principal component analysis (PCA) and wavelet transform (WT) as feature extraction methods were studied as conventional approaches for comparison. Deep learning approaches as modelling methods and feature extraction methods obtained good and equivalent performances to the conventional methods. The results illustrated that deep learning had great potential as modelling and feature extraction methods for chemical compositions determination in NIR-HSI.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yadan Zhang,Xiaoyu Zhang,Jie Zhou,Yuting Li,Tianhan Kai,Lin Zhang
Alzheimer's disease (AD) is predominantly characterized by cholinergic dysfunction, mitochondrial impairment, oxidative stress, and inflammation, primarily driven by amyloid-beta (Aβ) peptides. This study investigates the protective effects of Lycium ruthenicum Murray–derived exosome-like nanoparticles (LELN) in AD models using transgenic Caenorhabditis elegans ( C. elegans ). Findings showed that C. elegans effectively internalized LELN, which remained stable in vivo. Compared with untreated controls, treatment with 600 μg/mL LELN significantly extended the lifespan of CL4176 [ myo-3p ::Aβ 1–42 ] and CL2006 [ unc-54 /Aβ 1–42 ] worms by 34.78 % and 34.85 %, respectively, and delayed Aβ-induced paralysis by 52.42 % and 42.72 %, respectively. Furthermore, LELN increased the chemotaxis index of CL2355 [ snb-1 ::Aβ 1–42 ] worms from 11.11 % to 55.56 %. Mechanistically, LELN reduced the levels of Aβ oligomers and monomers via the DAF-16 pathway, consequently alleviating AD-like symptoms in transgenic C. elegans . The effects of LELN include inhibiting acetylcholinesterase activity to mitigate cholinergic dysfunction, restoring mitochondrial membrane potential and adenosine triphosphate production to ameliorate mitochondrial dysfunction, and reducing oxidative stress and inflammation. Collectively, these results highlight the protective role of LELN against Aβ-induced AD pathology and underscore their potential as a therapeutic candidate for AD treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
·
2026
·Vol. 49
·期 9
·e70809
·
IF 3.5
Yonghua Bao,Weiyuan Chen,Xiaohong Zhou,Sudan Ye,Chunhui Fu
A multifunctional intelligent packaging film was fabricated by co-incorporating Lycium ruthenicum anthocyanins (LRAs) and curcumin (CUR) into a polyvinyl alcohol (PVA) matrix. Microstructural and spectroscopic analyses supported the successful incorporation of both pigments and their intermolecular interactions with the PVA matrix. Notably, the physicochemical and functional properties of the composite films varied with the LRA/CUR ratio. Specifically, the CUR-dominant formulation (1:2) exhibited the highest elongation at break (86.13%) and antioxidant activity (DPPH scavenging rate of 63%) among the binary formulations, together with stronger antibacterial activity. Conversely, the LRA-dominant film (2:1) exhibited greater color stability and the strongest ammonia response (maximum Δ E = 35.02). When applied to chilled mutton stored at 4°C for 8 days, the intelligent films displayed progressive color transitions from yellow-orange to reddish-orange as spoilage progressed. These chromatic variations were accompanied by increases in pH (from 6.42 to 7.41) and total volatile basic nitrogen (TVB-N) content (from 9.29 to 39.41 mg/100 g). Overall, the LRA/CUR-PVA films combined freshness indication with active preservation, demonstrating their potential for intelligent chilled-meat packaging.
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 10
·1247-1257
·
IF 2.073
Jun Sang,Bing Li,Ya-ya Huang,Qun Ma,Kang Liu,Cui-qin Li
This study aimed to extract and separate total anthocyanins from Lycium ruthenicum Murr. by combining deep eutectic solvents (DES) with macroporous resin chromatography and to develop green analytical methods for the determination of anthocyanins. Choline chloride/1,2-propanediol (1 : 2, mol mol−1) containing 10% water (v/v) was screened as the optimal extraction solvent, and extraction at 52 °C for 45 min using a liquid/solid ratio of 20 : 1 gave the maximum extraction yield of total anthocyanins (4.45 ± 0.07 mg g−1). According to the static/dynamic adsorption and desorption tests, LS-32 macroporous resin has the ability to separate anthocyanins from DES with a recovery ratio above 95%. An off-line heart-cutting two-dimensional HPLC-DAD/ESI-MS method was developed for the qualitative and quantitative analysis of L. ruthenicum anthocyanins using environmentally friendly mobile phases. Ethanol and a tartaric acid solution (0.3 mol L−1) were used as the mobile phases of the first-dimensional HPLC-DAD method, and 15 anthocyanin peaks were detected within 17 min. The second-dimensional HPLC-MS method was used to identify the main anthocyanin peaks in the first dimension. Method validation showed that the developed method was accurate, stable and reliable for the analysis of L. ruthenicum anthocyanins. The present study provides an excellent alternative green method for the preparation and determination of anthocyanins from plant material.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 21
·2300090
·
IF 3.1
Qiangqiang Jia,Zufan Yang,Qian Wang,Haishan Yang,Xiaofeng Tang,Hongyang Zhang,Lingling Cao,Gong Zhang
It has been proved that purine metabolites are implicated in various biological syndromes and disorders. Therefore, the realization of panoramic detection of purine metabolites will be of great significance to the pathogenesis of purine metabolic disorders. In the present study, an ultra-high performance liquid chromatography with tandem mass spectrometry method was developed for the comprehensive quantification of purine metabolites in rat plasma. The 17 purine metabolites were separated and quantified in the short running time of 15 min. The proposed method was strictly validated by applying SeraSub solution as a matrix and proved to be linear (R 2 ≥ 0.9944), accurate (the recoveries of all analytes ranged from 85.3% to 103.0%, with relative standard deviation values ≤ 9.3%), and precise (the intra- and inter-day precisions were less than 10.8% and 12.4%, respectively). The method was then successfully applied to the qualification of the endogenous purine metabolites in acute gouty arthritis rats, as well as colchicine and anthocyanin-intervened rats. Results showed that uric acid, xanthine, hypoxanthine, and xanthine were considered the key factors of acute gouty arthritis. The established method and measurement of purines in rat plasma might help the investigation of the action mechanisms between purine disorders and related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）