TALANTA
·
2020
·
IF 5.339
Muhammad Mujahid Ali,Dilshad Hussain,Bin Xu,Tangqiang Sun,Zhenxia Du
Cis -diol compounds are class of biomolecules including nucleosides , glycoproteins , saccharides , and nucleotides, which play vital roles in various biological processes. Due to low abundances of these species in the complex biological samples, their identification and analysis is difficult. Boronate affinity materials are commonly used for the isolation and enrichment of cis -diol compounds, due to their unique, facile and selective enrichment mechanism. In this study we report a selective approach to extract nucleosides, glycopeptides and glycoproteins using boronic acid functionalized GMA-MAA-DVB polymer. This novel polymer, reported for the first time in proteomics, have high BET surface area (132.8447 m 2 g −1 ) which contribute to efficient enrichment and average pore size (20.3449 nm) to facilitates the nano confinement effect for strong interactions. Hydrophilic character of methacrylic acid and diethylenetriamine , along with inherent affinity of boronic acid for glycosylated biomolecules result in selectivity up to 1:500 for peptides and 1:1000 for glycoprotein. Binding constant for cis -diol compounds are in the range of 10 −4 to 10 −6 M and theoretical binding capacity up to 85 mg g −1 for HRP and 180 mg g −1 for IgG, respectively. Furthermore, boronic acid functionalized polymer (BFP) enrich glycoproteins and glycopeptides in range of 1 pg mL −1 and 0.04 ng mL −1 with S/N ≥ 3. Finally, material is applied to enrich the glycoproteins from healthy human saliva sample and six glycoproteins are identified.
研究领域:
生物化学
·
蛋白质组学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 34
·8788-8795
·
IF 5.256
Xiaofang Qi,Jing Peng,Dongyan Tang,Ning Wang,Hongyun Zou
Molybdenum oxide nanomaterials have received increasing attention in recent years for their strong localized surface plasmon resonance (LSPR) absorption in the near-infrared (NIR) region and pH-dependent degradability. However, the rapid degradation of MoOx at physiological pH values would decrease the NIR photothermal effect. Here, we designed and prepared PEGMa–MoOx/p(NIPAM-co-MAA) microgels (NCs) exhibiting adjustable degradation. For that purpose, PEGMa (Mn = 400) modified MoOx was synthesized through a simple hydrothermal process. Then, PEGMa–MoOx with existing CC groups was continually polymerized with NIPAM and MAA through emulsion polymerization. MAA was introduced to provide an acidic microenvironment (pH at 3–5) to control the degradation rate of PEGMa–MoOx. The photothermal efficiency of the NCs could be maintained at 54% even after 14 days. Meanwhile, the NCs also displayed a pH-dependent thermal response. Moreover, PEGMa was added again in the emulsion polymerization process to elevate the photothermal efficiency, and the irradiation result indicated that the NCs could increase the temperature by 27 °C. The complex microgel integrates pH/temperature/photothermal triple response characteristics and exhibits huge potential for chemo-photothermal combination cancer therapy.
研究领域:
材料科学
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生物医学工程
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纳米医学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Yuanpeng Cai,Danyang Li,Yufei Ling,Huihui Jiang,Hui Liu
A novel triple-responsive composite hydrogel, p(NIPAM-co-MAA-co-SPMA), was designed and synthesized via free radical polymerization, incorporating N-isopropylacrylamide (NIPAM) for temperature responsiveness, methacrylic acid (MAA) for pH sensitivity, and spiropyran methacrylate (SPMA) for light responsiveness. This study aimed to develop a novel stimuli-responsive sustained release drug delivery system. The composition and structure of the hydrogel were characterized by nuclear magnetic resonance spectroscopy (NMR), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM). Doxorubicin (DOX) was used as a model drug to evaluate loading and release behavior. The hydrogel achieved a drug loading content (DLC) of 25.7% and a drug loading efficiency (DLE) of 74.3% and enabled controlled release with approximately 89% cumulative DOX release within 6 h under combined thermal, acidic, and ultra-violet light stimuli. Cytotoxicity assays and cellular uptake studies confirmed the excellent biocompatibility of the hydrogel and its efficacy in suppressing cancer cell growth. These results highlight the potential of p(NIPAM-co-MAA-co-SPMA) as an intelligent platform for on-demand targeted anticancer therapy.
研究领域:
高分子材料科学
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肿瘤学
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药物递送系统
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生物医学工程
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智能药物释放
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纳米医学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 24
·10618-10625
·
IF 3.591
Qianrui Xu,Yang Wang,Pengcheng Gao,Yong Jiang
Fluorescent nanobeads are widely used due to their advantages of visualization, sensitivity and the quantitative measurement of target analytes. In terms of their applications, the stability and biocompatibility of fluorescent nanobeads are very important. In this work, “pomegranate”-like QD/SiO2/poly(St-co-MAA) (QSIP-COOH) nanobeads were synthesized by two-step strategies. First, quantum dots (QDs) were covered by a silica layer using the reverse hydrolysis method to form a core–shell structure like the seed and flesh of a pomegranate, and then a poly(St-co-MAA) layer was formed as the skin of the pomegranate. The structure and morphology of nanobeads were investigated by FTIR, TG, TEM and HRTEM. The results confirmed that QSIP-COOH nanobeads had a pomegranate structure with a QD core, an intermediate silica shell, and a carboxylated polystyrene outermost shell. TEM and HRTEM results showed that the size of the typical product was about 170 nm with the QDs of about 8 nm and the MPS-modified QD/SiO2 (QSI-CC) of about 27 nm, respectively. The in vitro cytotoxicity test showed that nanobeads had desirable stability and good biocompatibility, which made them applicable for various bioassays and diagnostics. Here, QSIP-COOH fluorescent nanobeads were applied to immunochromatography test strip (ICTS) and the result proved that QSIP-COOH fluorescent nanobeads could be used for the sensitive detection of immune human chorionic gonadotropin (HCG).
研究领域:
材料科学
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纳米技术
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生物医学工程
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分析化学
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DOI
高影响因子
OPTICAL MATERIALS
·
2020
·
IF 2.779
Aiqin Zhang,Zhi Wang,Weize Hong,Yining Zhang,Jiandong Guo,Yongchao Wang,Yanqin Miao,Husheng Jia,Hua Wang,Bingshe Xu
A novel bonded phosphor poly(Eu(TTA) 2 (phen)MAA-co-VA) (Eu-PVA, for short) was prepared employing the technology of coordination-polymerization-alcoholysis. The bonded Eu-PVA fluorescent nanofibers were prepared by electrospinning, having the initial decomposition temperature of 290 °C higher 51 °C than that of the doped nanofibers. A relationship is established between the fiber morphology and the fluorescence intensity of the bonded fiber Eu-PVA, predicting fluorescence quenching according to the morphological evolution. Compared with the corresponding fluorescent phosphors, both doped and bonded fluorescent fibers exhibit obvious fluorescence enhancement effects in that nanofibrosis increase the specific surface of the fibers and decrease the diffuse reflection and the light loss owing to the smoother surface of the fibers than that of the phosphors. Furthermore, security paper with both masking and security has been fabricated applying the bonded fluorescent nanofibers into pulp, being extended to the development of banknotes, bills, and securities.
研究领域:
材料科学
·
化学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 28
·4402-4412
·
IF 5.047
Mengwei Zou,Rongrong Jin,Yanfei Hu,Ying Zhang,Haibo Wang,Gongyan Liu,Yu Nie,Yunbing Wang
Indomethacin (IND) is a nonsteroidal anti-inflammatory drug (NSAID), which is widely used to relieve inflammation. However, general eye drops cannot reach lesions efficiently due to the presence of complicated and special biological barriers in the eye. Repeated administration of this drug for chronic ophthalmic diseases also showed poor patient compliance. Herein, an in situ injectable thermo-sensitive hydrogel of indomethacin-conjugated polymer was fabricated to overcome these crucial problems. The poly(NIPAAm-co-MAA-co-HTI) (PNMHTI) hydrogel, composed of N-isopropylacrylamide (NIPAAm), methacrylic acid (MAA), and 2-hydroxylethyl methacrylate-g-poly(trimethylene carbonate)-indomethacin (HEMA-g-PTMC-IND) (HTI), was synthesized by radical polymerization of NIPAAm, MAA and HTI. According to the rheology investigation, the sol–gel conversion temperature of the hydrogels was around 33 °C, and the sol–gel transition occurred at physiological temperature (37 °C). The release of indomethacin from the injectable hydrogels was sustained for over 2 weeks in phosphate buffer saline with esterase due to the sustained biodegradable behavior by the hydrolytic cleavage of the residual PTMC chains. In addition, these hydrogels displayed excellent biocompatibility against mouse fibroblasts, reduced cytotoxicity for indomethacin and a significant anti-inflammatory effect against macrophages. Uveitis was successfully established in New Zealand rabbits, and the PNMHTI hydrogels were injected into the vitreous body. Histopathological examination of retinas confirmed the good biocompatablity and anti-inflammatory effects of PNMHTI hydrogels in vivo. Overall, the PNMHTI hydrogels reported herein offer an attractive biomaterial-centered treatment option for uveitis.
研究领域:
药学
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材料科学
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眼科学
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生物医学工程
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DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2014
·Vol. 29
·期 6
·869-875
·
IF 1.662
Yonggang Chen,Junhua Meng,Jili Zou,Jing An
Hordenine is an active compound found in several foods, herbs and beer. In this work, a novel sorbent was fabricated for selective solid-phase extraction (SPE) of hordenine in biological samples. The organic polymer sorbent was synthesized in one step in the plastic barrel of a syringe by a pre-polymerization solution consisting of methacrylic acid (MAA), 4-vinylphenylboronic acid (VB) and ethylene glycol dimethacrylate (EGDMA). The conditions for preparation were optimized to generate a poly(MAA-VB-EGMDA) monolith with good permeability. The monolith exhibited good enrichment efficiency towards hordenine. By using tyramine as the internal standard, a poly(MAA-VB-EGMDA)-based SPE-HPLC method was established for analysis of hordenine. Conditions for SPE, including volume of eluting solvent, pH of sample solution, sampling rate and sample volume, were optimized. The proposed SPE-HPLC method presented good linearity ( R 2 = 0.9992) within 10–2000 ng/mL and the detection limits was 3 ng/mL, which is significantly more sensitive than reported methods. The method was also applied in plasma and urine samples; good capability of removing matrices was observed, while hordenine in low content was well extracted and enriched. The recoveries were from 90.6 to 94.7% and from 89.3 to 91.5% for the spiked plasma and urine samples, respectively, with the relative standard deviations <4.7%. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
分析化学
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生物分析
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分离科学
·
药物分析
✓ 已验证
DOI
高影响因子
Pigment & Resin Technology
·
2024
·
IF 1.3
Zhichuang Qi,Jingshan Chen,Zhangmi Huang,Chunyan Hu,Baojiang Liu
PurposeThis paper aims to prepare Poly(Styrene-Butyl acrylate-Methacrylic acid) @Poly Gallic acid-Fe3+photonic crystal composite inks [P(St-BA-MAA)@PGA-Fe3+PCCI, @ means the PGA-Fe3+is loaded on the microspheres] and construct noniridescent structural colors on fabric substrates, with the goal of improving the visibility of structural colors.Design/methodology/approachP(St-BA-MAA)@PGA-Fe3+PCCI were prepared by coating P(St-BA-MAA) microspheres with a metal-polyphenol network formed by gallic acid (GA, C7H6O5) and Fe3+. The assembly effects of the inks were explored under different conditions, including pH, temperature, concentration and surface tension. The optimal self-assembly conditions of the inks were determined using the controlled variable method.FindingsThe results demonstrated the successful preparation of P(St-BA-MAA)@PGA-Fe3+PCCI. The metal polyphenol network film composed of GA and Fe3+was successfully coated on the surface of P(St-BA-MAA) seed microspheres. The assembly mechanism of the inks was investigated, indicating that at a diethylene glycol (DEG, C4H10O3) concentration of 0.3 wt% and pH of 7, bright noniridescent structural colors could be formed on fabric surfaces after self-assembly by PCCI at 60 °C for 10 min. Furthermore, the mechanical fastness of the structural colors was enhanced due to the adherence of the soft shell composed of P(St-BA-MAA) and GA.Originality/valueUtilizing a cost-effective approach and a diverse array of readily available raw materials, we have successfully prepared P(St-BA-MAA)@PGA-Fe3+PCCI, which boasts superior performance and offers fabrics a range of unique coloring styles. This innovation paves the way for potential applications of structural colors in practical production, thereby broadening their realm of utility.
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DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Qichao Ye,Chen Men,Lili Tian,Yuxin Liu,Lei Zhan,Yuan Fang Li,Cheng Zhi Huang,Shu Jun Zhen
Conventional methods for the detection of the sulfur mustard poisoning metabolic marker, thiodiglycol (TDG), require expensive instruments and reagents as well as professional operators. To address these problems, a novel test strip based on a molecularly imprinted sensitive membrane (MIM) was developed in this work for point-of-care (POC) detection of TDG. The TDG test strip was prepared conveniently by coating molecular imprinted polymers (MIPs) on a nitrocellulose membrane. When the sample contained TDG, the MIPs could specifically bind with TDG. A great number of AuNPs (AuNPs) could then be adsorbed on the test strip via the formation of an Au–S bond between TDG and AuNPs, giving the test strip the obvious red color of AuNPs. In the absence of TDG, the test strip exhibited much lighter color because it could not adsorb AuNPs. By monitoring the color change of the test strip, TDG could be detected from 1.0 ng/mL to 100.0 μg/mL with a detection limit of 0.23 ng/mL (3σ) under the optimal conditions (the molar ratio of TDG to MAA was 1:2; the eluent was chloroform; the elution time was 50 min; the reaction time between MIPs and TDG was 15 min; the adsorption time of AuNPs was 40 min; the temperature of the reaction system was 35 °C). This method has excellent selectivity and has been used to detect TDG in urine, showing great potential for POC detection of TDG in clinical samples.
研究领域:
分析化学
·
生物传感器
·
即时检测
·
毒理学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yafang Shen,Fei Jia,Aoming Liang,Yawen He,Yaping Peng,Huang Dai,Yingchun Fu,Jianping Wang,Yanbin Li
Exploring new functions of nanomaterials can help facilitate the development of biosensors for the detection of antibiotics. Herein, a new detection modality based on monovalent antigen-induced aggregation (MAA) of immunomagnetic beads (IMBs) was proposed for rapid and label-free detection of enrofloxacin (ENR), which endowed IMBs with the abilities of both sample separation and signal generation. In the presence of ENR, the initially well-dispersed IMBs were aggregated and the degree of aggregation was in a concentration-dependent manner. After exploring the mechanism underlying IMB aggregation and investigating the key parameters affecting it, a label-free biosensing platform was developed for rapid and sensitive detection of ENR. Based on the significant differences in the magnetic separation speed and size between the aggregated and well-dispersed IMBs, two methods were proposed for quantitatively determining ENR, i.e., measuring the turbidity of the IMB supernatant after magnetic separation for a given time and visualizing and calculating the grayscale value of the aggregated IMBs trapped on the surface of a nitrocellulose membrane. A three-dimensional (3D)-printed syringe was designed and fabricated for automatic filtration of IMBs. This immunosensor allowed for sensitive detection of ENR in less than 15 min without any labels. It exhibited a satisfactory limit of detection of 0.79 ng mL–1 and showed the feasibility for ENR detection of spiked chicken meat with recovery rates ranging from 74.8 to 98.3%. The MAA immunosensor can act as a promising tool to detect trace levels of ENR and has the potential to be applied to complex food samples.
研究领域:
分析化学
·
生物传感
·
纳米技术
·
食品安全
·
环境科学
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产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2019
·
IF 2.781
Jun Wang,Na Huang,Qi Peng,Xiaoyan Cheng,Wuke Li
In this paper, the luminescent temperature/pH dual-responsive nanoparticles based on lanthanide-polyoxometalates were fabricated as a controllable drug delivery system. In the drug delivery carrier, the mesoporous silica acted as the core, and the poly (N-isopropylacrylamide-co-methacrylic acid) and lanthanide-polyoxometalates served as the dual-responsive shell and luminescent labeling, respectively. Composite hydrogels exhibited red luminescence under UV irradiation, which was visible to the naked eye. The releases of doxorubicin hydrochloride (DOX) from the composites were temperature/pH-dependent. DOX can be quickly released in an acidic environment and at high temperatures. Moreover, the drug delivery carrier showed minimal toxicity to HeLa cells and exhibited good biocompatibility. However, DOX loaded drug-delivery system showed the significant toxicity to HeLa cancer cells in vitro. Cell imaging has shown that lanthanide polyoxometalates composites can be used as bioimaging materials for cell monitoring. As a result, the drug delivery systems based on lanthanide-polyoxometalates have potential applications in the field of biomedical science.
研究领域:
生物医学
·
材料科学
·
药理学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2018
·Vol. 56
·期 8
·679-686
·
IF 1.037
Shi Jie-Hua,Zhao Li-Hua,Shen Guo-Feng
A poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolithic column was prepared in situ for high performance liquid chromatography and characterized utilizing scanning electron microscopy, thermodynamic parameters and linear solvation energy relationship methodology. The results revealed that there were many microglobules forming the larger cluster in poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolith prepared under the selected preparation conditions, which composed continuously aligned macroporous channels through the monolith skeleton. The poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolithic column had a good permeability, a high mechanical stability. The interaction model for each probe molecule on the poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolithic column was invariable within the studied temperature range due to an excellent linear relationship between lnk and 1/T for each probe molecule. The chromatographic retention for each probe molecule on the poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolithic column was an enthalpy-driven process due to |∆H°| > |T∆S°| and ∆G°<0. The main interaction forces of the poly(Methacrylate-co-Ethyleneglycol dimethacrylate) monolith with probe molecules contain hydrogen bonding interaction, hydrophobic interaction and dipole-dipole interaction. However, the contribution of each interaction was in the order of hydrogen bonding acidity > hydrophobic interactions > dipole-dipole interaction > hydrogen bonding basicity. In addition, the poly(Methacrylate-co-Ethyleneglycol dimethacrylate)monolithic column is suitable for the separation of both nonpolar and polar compounds.
研究领域:
分析化学
·
色谱技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）