APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Shuiping Tang,Xiaohuan Qin,Yuanxia Lv,Kun Hu,Shulin Zhao
In this work, according to the structure of three perfluoroalkyl sulfonates (PFASs) (perfluorobutylenesulfonic acid (PFBS), perfluorohexanesulfonic acid (PFHxS), and perfluorooctylsulfonic acid (PFOS)) and the property of a medium, we synthesized good-compatibility and stable cationic covalent-organic frameworks (C-COFs). They were used as adsorbents to absorb and detect analytes with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The structures of the C-COFs were characterized. Enrichment parameters, including adsorbent amount, enrichment time, pH, and type and volume of dispersion solution, were optimized to achieve the best enrichment efficiency. After the enrichment, the loaded C-COFs were directly analyzed by MALDI-TOF MS. The results indicated that the C-COFs had a high surface area (1105 m 2 /g) and microporous structure (1.1 nm); they are rapid and highly efficient for tested PFASs adsorption. The equilibrium adsorption capacity of the C-COFs for PFBS was 334.46 mg g −1 . Further, the detection limit for PFBS, PFHxS, and PFOS was 0.001, 0.01, and 0.3 ng mL −1 , respectively. Moreover, adsorption and ionization mechanism was discussed. Owing to high selectivity and sensitivity, high adsorption performance, good reproducibility, and satisfactory recoveries, the method was successfully applied to detect PFASs in a real sample.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Yuean Chu,Mingyue Yu,Yunxing Li,Suoxian Yang,Hongyan Bi
Tracement of the origin of seafoods and aquatic products is an important issue for avoiding the falsification of production regions of aquatic products. Herein, by analyzing black tiger shrimp ( Penaeus monodon ) as a model of aquatic products, we demonstrated a method to trace the origin of aquatic products by analyzing the gut microbiome of black tiger shrimp ( Penaeus monodon ) collected from three commercial farms in China, via matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Multivariate analysis was executed on the obtained mass spectrometric datasets of bacteria extracted and collected from shrimp samples with different production regions. The gut microorganisms of black tiger shrimps can be identified by scoring the gut microbiota of black tiger shrimps collected from three producing areas. The black tiger shrimp from three production regions were found with distinguished characteristics of bacterial prevalence in their digestive gland microbiota. The dominant bacteria genus in digestive gland microbiota from black tiger shrimp samples from the three production regions are completely different. Single-blind shrimp samples with unknown production region was successfully screened by comparing the detected MS data in different ways. This strategy can trace the production region of the analyzed shrimp samples in a quick and efficient mode. The application of the present protocol was further demonstrated by analyzing the biometric species from gill tissue of Chinese mitten crab ( Eriocheir sinensis ) samples collected from crab-producing areas in Jiangsu Province (China), that is, Taihu Lake, Gaoyou Lake, and Hongze Lake for tracing their production area. It is expected to extend the presently developed method to provide clues for the origin traceability of other aquatic products.
研究领域:
水产养殖
·
食品科学
·
微生物学
·
质谱分析
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
FuKai Li,Min Wang,Jian Zhou,MengRui Yang,TongTong Wang
Novel boronic acid-functionalized magnetic multi-walled carbon nanotubes with flexible branched polymer (Fe 3 O 4 @MWCNTs@ε-PL@BA) nanocomposites were fabricated and applied as the desorption/ionization matrix for the MALDI-TOF-MS determination of low molecular weight flavonoids . The prepared nanocomposite was systematically characterized by various techniques. Compared to the traditional organic matrix, the proposed Fe 3 O 4 @MWCNTs@ε-PL@BA matrix has excellent ionization efficiency and low-background noise interference due to the MWCNTs unique electron-phonon interaction and the high introduction density of boronic acid functional groups. Good sensitivity and ultra-high salt tolerance of the Fe 3 O 4 @MWCNTs@ε-PL@BA-assisted MALDI-TOF-MS were permitted for the determination and quantification of flavonoids in actual samples. Noticeably, the limits of detection (LODs) for the target flavonoids were in the range 17–33 nM. The relative standard deviations (RSDs) of spot-to-spot and sample-to-sample (n = 10) were ≤ 9.8% and ≤ 10.1%, respectively. Furthermore, the wide linear ranges (0.1 – 500 µg/mL) and satisfactory calibration plot coefficients (R 2 > 0.99) of flavonoids were achieved by MALDI-TOF-MS with the Fe 3 O 4 @MWCNTs@ε-PL@BA matrix. Good recoveries (92–105.5%) were achieved for the target flavonoids in practical food samples. Hence, the prepared Fe 3 O 4 @MWCNTs@ε-PL@BA nanocomposites have applications in the selective and efficient capture of target flavonoids active biomolecules coupled with MALDI-TOF-MS determination in actual samples.
研究领域:
分析化学
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材料科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2022
·Vol. 24
·期 17
·10394-10407
·
IF 3.945
Jing Xiao,Cong Wang,Bai-Qi Feng,Tian-Yu Liu,Shao-Yi Jia,Hai-Tao Ren,Yong Liu,Song-Hai Wu,Xu Han
Although synthesis of oligoaniline (OANI) by persulfate and aniline has been investigated in the recent years, the impact of phenol on the synthesized soluble OANI is still not clear. In this study, our results indicate that phenol and pH mediate the production of the blue water-soluble OANI (OANIblue) in the reaction between sodium persulfate (SPS) and aniline under alkaline conditions, and the yields of OANIblue increase with increasing concentrations of phenol and pH values. Quenching experiments rule out the contributions of SO4˙− and ˙OH to aniline oxidation and imply that the non-radical activation of SPS is an important pathway in the formation of OANIblue. MALDI-TOF-MS analysis indicates that phenol apparently inhibits the polymerization degree of aniline in that the molecular weights of OANIblue gradually decrease from 1586.4 to 684.6 when phenol is increased from 0 to 2.0 mM. FTIR and Raman analyses confirm the structure of aniline oligomers in OANIblue and indicate that phenol inhibits the phenazine-like structure in OANIblue and facilitates the transformation of benzenoid rings to quinoid rings in the oxidation products. However, simultaneous activation of SPS by phenol and aniline is likely to occur in the reaction system with the formation of PhNH˙, as indicated by DFT calculations. The high scavenging reactivity of phenol towards both PhNH2˙+ and PhNH˙ implies that PhNH2˙+ and PhNH˙ are not the intermediates in the formation of OANIblue. DFT calculations also reveal that apart from the one-electron transfer pathway between aniline and SPS, the two-electron transfer pathway is also likely to occur in the presence of phenol, resulting in the formation of PhNH+/PhN˙˙ without producing PhNH2˙+ and PhNH˙. The produced PhNH+/PhN˙˙ intermediates then couple with aniline, PhNH+, aminophenyl sulfate and its hydrolysate to form dimers, trimers, oligomers, and eventually OANIblue. This study not only describes a novel method to prepare water-soluble OANI, but also gives new insight on the importance of phenol in the production of OANIblue.
研究领域:
化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 11
·2891
·
IF 3.098
Xiao Chen,Jia Xiong,Shenlin Huang,Xun Li,Yu Zhang,Liping Zhang,Fei Wang
The proanthocyanidins from ethanol extracts (80%,v/v) ofAcacia mearnsii(A. mearnsii) bark on chemical-based and cellular antioxidant activity assays as well as carbolytic enzyme inhibitory activities were studied. About 77% of oligomeric proanthocyanidins in ethanol extracts ofA. mearnsiibark were found by using normal-phase HPLC. In addition, HPLC-ESI-TOF/MS and MALDI-TOF/TOF MS analyses indicated that proanthocyanidins fromA. mearnsiibark exhibited with a degree of polymerization ranging from 1 to 11. These results of combined antioxidant activity assays, as well as carbolytic enzyme inhibitory activities of proanthocyanidins fromA. mearnsiibark, indicated an encouraging antioxidant capacity for the high polyphenol content and a potential for use as alternative drugs for lowering the glycemic response.Keywords:proanthocyanidins;HPLC/MS;MALDI-TOF/MS;antidiabetic;antioxidant;degree of polymerization
研究领域:
药理学
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生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Xi-Ming Wang,Zhi-Yong Guo,Yue Zhang,Ming-Li Chen,Jian-Hua Wang
Fe x O y and ZrO 2 nanoparticles co-doped layered porous polyimide , polyimide-Fe x O y -ZrO 2 is prepared with a one-step strategy, shortly termed as PI-Fe x O y -ZrO 2 . The layered and porous structure of the polymer offers a supported platform for metallic oxide anchoring, exhibiting a mesopore size of 3.93 nm and providing a surface area of 198.47 m 2 g −1 . The metallic oxides were uniformly and highly dispersed in the PI-Fe x O y -ZrO 2 nanocomposite with percentages of 15.81 and 20.53 wt% for Fe and Zr, respectively. The magnetic Fe x O y provides driving force for rapid separation. The high doping of ZrO 2 facilitates effective enrichment of phosphopeptides, even at a very low mass ratio of 1:1000 for tryptic digest of phosphopeptides/non-phosphopeptides, e.g., β-casein/BSA in this particular case. In addition, the PI-Fe x O y -ZrO 2 nanocomposite exhibits better adsorption performance to phosphopeptides with respect to commercial titanium dioxide nanoparticles . The effectiveness of low-abundant phosphopeptides isolation and enrichment from human serum is further identified and demonstrated by means of MALDI-TOF MS and LC-ESI-MS/MS.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
CHINESE CHEMICAL LETTERS
·
2018
·
IF 2.631
Zengnan Wu,Ning Xu,Weiwei Li,Jin-Ming Lin
The performance of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) is frequently compromised by the high limited of detection, heterogeneous distribution of matrix and analyte deposits and time-consuming drying time during the conventional drop drying procedure. Here, we describe a method based on membrane separation technology to assist in the drying of nanomaterials to rapidly make homogeneous sample spots and to enhance the mass signal. We demonstrate that the sample can be dried evenly in a few seconds and MS signal can be greatly optimized by using membrane separation technology. Enrichment with nanomaterials greatly enhances analyte signal strength while membrane separation method avoids the time-consuming requirements of searching for “sweet spots”.
研究领域:
分析化学
·
质谱技术
·
纳米技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2017
·Vol. 10
·期 4
·459-466
·
IF 1.9
Lingyi Zhang,Bingbing Wang,Shulei Wang,Weibing Zhang
In this work, recyclable trypsin immobilized magnetic nanoparticles based on hydrophilic branched polyethylenimine (PEI) modification were synthesized under mild conditions. The amount of the immobilized enzyme was increased greatly by introducing branched PEI onto poly(methacrylic acid) (PMAA) modified Fe3O4 microspheres (denoted as Fe3O4@PMAA@PEI). Trypsin, a model enzyme for this study, was immobilized by Cu2+ with metal chelation to Fe3O4@PMAA@PEI. Compared with Fe3O4@PMAA nanoparticles, the abundant amino groups of the PEI layer not only increased the amount of the immobilized enzyme, but also improved the hydrophilicity of the material to avoid nonspecific protein adsorption. The performance of this material was evaluated by digesting bovine serum albumin (BSA), myoglobin and cytochrome C, followed by MALDI-TOF-MS analysis. Compared with in-solution digestion, not only the sequence coverage was improved (70.0 ± 3.1% vs. 40.0 ± 2.3% for BSA, 92.0 ± 2.4% vs. 81.0 ± 0.8% for myoglobin and 90.0 ± 3.0% vs. 56.0 ± 1.8% for cytochrome C), but also the digestion time was decreased from 24 h to 10 min. Furthermore, the relative standard deviation values (RSDs) of the sequence coverage of BSA were 3.0% for five consecutive digestions and 2.2% for particles from different batches (n = 3), showing good reproducibility. The Fe3O4@PMAA@PEI-trypsin can endure at least 7 times usage and still maintain good digestion performance after being stored at 4 °C for 25 days. When applied to complex samples, more proteins were identified from the extract of a rat liver than in-solution based digestion (582 vs. 496), with a shorter reaction time of 10 min.
研究领域:
生物化学
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材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 98
·96210-96222
·
IF 3.289
Xing-yu Long,Jia-yuan Li,Dong Sheng,Hong-zhen Lian
The reversible phosphorylation of proteins plays a key role in numerous biological processes, while abnormal phosphorylation is a cause or consequence of many human diseases. Selective enrichment and MALDI-TOF MS or MS/MS characterization and identification of endogenous phosphopeptides fished-out from human saliva sample were achieved using simple and low-cost iron oxide magnetic nanoclusters (Fe3O4 MNCs) affinity probe in this work. The physicochemical properties of the prepared MNCs material was characterized by SEM, TEM, XRD, EDS, SQUID, etc. Based on their merits of high affinity, rapid separation, good biocompatibility, excellent dispersibility and superior specificity for capturing phosphopeptides, the performances of Fe3O4 MNCs were assessed by selectively and efficiently isolating phosphopeptides from standard phosphoprotein and actual non-fat milk tryptic digests, and intricate human saliva, respectively. Moreover, we demonstrated that the probe can be applied for rapid and effective purification and enrichment of phosphoproteins. The Fe3O4 MNCs affinity probe, which possesses the merits of integration of solid Lewis acids function and magnetic property, together with the “double economical” (both time and money) synthesis strategy, should be a promising material for the large scale investigation of endogenous phosphoproteomics.
研究领域:
生物化学
·
蛋白质组学
·
医学诊断
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DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Jiarui Li,Shuai Tang,Hao Wang,Xinhua Guo
The rapid and selective detection of biomarkers for the diagnosis of related diseases still remains a significant challenge due to the complexity of biosamples. Carboxylesterase (CES) is one of the most abundant serine hydrolases in the liver, playing an important role in the metabolism of xenobiotic compounds. In particular, the upregulation of CES is closely associated with hepatocellular carcinoma (HCC), which is a potential biomarker to improve the diagnostic accuracy. Herein, based on the “substrate-hydrolysis enzymatic reaction” strategy, we developed a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) probe, named QF, for the quantitative analysis of CES activity. The QF contained an amide group serving as the enzymatic recognition moiety, and a quinoline tag was introduced to improve the ionization efficiency of both the substrate and its hydrolysis product. Therefore, the CES activity could be directly quantified by the relative intensity ratio of the product and substrate via MALDI-TOF MS without any additional internal standard. Owing to its operating simplicity, high-throughput capacity and quantification accuracy, the proposed method was successfully applied for CES activity quantification, inhibitor screening, and CES activity detection in the cell lysates. This study provides a tool for the rapid detection of CES activity and CES drug development.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2017
·Vol. 55
·期 15
·2537-2543
·
IF 2.952
Jiatao Wu,Chunyan Tan,Xinwen Zhou,Ying Tan,Pengyuan Yang,Yuyang Jiang
Molecular weights of seven poly(phenylene ethynylene)-based water-soluble conjugated polyelectrolytes (CPEs) obtained through Sonogashira coupling are determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A standard sample preparation protocol is developed to characterize the seven CPEs using 2,5-dihydroxybenzoic acid as the matrix (M) and AgTFA as the cationization reagent (CR). High-quality MALDI mass spectra are obtained at volume mixing ratios (CPE/M/CR) of 5/5/1 for anionic polymers (P1–P4) and 5/50/1 for cationic polymers (P5–P7). Molecular weight, molecular weight distribution, and end-group information are analyzed. The effects of molecular weight of CPEs on optical and quenching properties are also studied. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017 , 55 , 2537–2543
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 1
·e70010
·
IF 3.4
Wanyi Zhang,Qiuqi Peng,Linhan Yuan,Caiwen Wu,Mengqi Wang,Hongbo Li,Hongjuan Li,Jinghua Yu
Infant formulas are constantly being updated and upgraded, and N-glycans are functional glycans that have not been fully exploited to date. Commercial whey protein materials are often used as basic ingredients in infant formulas. Therefore, it is important to study N-glycans in commercial whey protein materials. We used matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and ion chromatography to analyze N-glycans in bovine lactoferrin (Lf), whey protein isolate (WPI), whey protein concentrate 70 (WPC 70), goat whey protein powder 50, demineralized whey powder 90 (D90), and desalted goat whey powder. The results showed that 30, 6, 28, 16, 8, and 9 N-glycans were found in Lf, D90, desalted goat whey powder, WPI, WPC 70, and goat whey protein powder 50, respectively. A total of four structures of N-glycans were detected in this study. Only bovine Lf and WPC 70 contained fucosylated and sialylated binding (SFN-type) glycan structures. Regarding content, WPC 70 showed the highest yield of 14.5 mg/g, and the degree of sialylation was higher than fucosylation. This study provides a potential basis for the future use of commercial whey protein materials in dairy products such as infant formula.
研究领域:
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）