SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Shi-Yun Cen,Yi-Ge Feng,Jian-Hong Zhu,Xiao-Yu Wang,Ai-Jun Wang,Xiliang Luo,Jiu-Ju Feng
Advanced nanomaterials have attracted ever-growing interest in design and construction of electrochemical immunosensors, which hold great promise to improve sensitivity in bioanalytical system. In this work, ultra-thin AuPdCu alloyed nanowire networks (NWNs) were controllably synthesized by an eco-friendly one-pot aqueous approach with 4-aminopyridine as a structural directing agent, which served as signal amplifiers in construction of an ultra-sensitive label-free electrochemical immmunosensor for creatine kinase-MB (CK-MB) assay, taking the virtues of the large specific surface area, high electronic conductivity, and good biocompatibility. Importantly, the AuPdCu NWNs efficiently catalyzed the reduction of hydrogen peroxide (H 2 O 2 ) and thereby enhanced the detection responses. Under the optimal conditions, the as-fabricated immmunosensor showed a wide linear range of 0.001–2000 ng mL –1 for CK-MB detection, along with a low limit of detection (LOD = 0.88 pg mL –1 , S/N = 3). Thereby, this research was successfully applied for CK-MB quantitation in serum samples with acceptable results, which would provide some valuable guidelines for monitoring other cardiac markers in clinical applications.
研究领域:
材料科学
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生物医学工程
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分析化学
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DOI
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2021
·
IF 1.321
Yuqin Liao,Ruiyun You,Min Fan,Shangyuan Feng,Dechan Lu,Yudong Lu,Yuqin Liao,Ruiyun You,Min Fan,Shangyuan Feng,Dechan Lu,Yudong Lu
Nicotinamide adenine dinucleotide (NADH) is an important coenzyme involved in various metabolic processes of living cells. As an important biomarker, NADH is associated with breast cancer and Alzheimer’s disease. In this paper, silver plated gold core–shell nanoparticles containing Raman signal molecules were synthesised on the basis of bare gold. Using the Raman peak corresponding to the 4-mercaptobenzonitrile (MB) silent region C≡N vibration for quantification, while avoiding competition with the precious metal surface binding site to be measured, it can also be free from the interference of endogenous biomolecules. On the one hand, it can correct the working curve, on the other hand, it can avoid competing with the binding site. Compared with the core–shell structure prepared here, the limit of detection (LOD) for NADH was only 10−5 M for bare gold and the LOD for the core–shell structure prepared on the basis of bare gold was 3.3 × 10−7 M. In terms of correction, with Rhodamine 6G (R6G) as a Raman signalling molecule, the R2 value before SERS detection and correction is only 0.9405, and the R2 value after correction increases to 0.9853. The unique fingerprint peak of SERS was used to realise the quantitative detection of NADH, which realizes the detection of NADH in complex biological samples of serum and provides the possibility for expanding the early diagnosis of breast cancer.
研究领域:
分析化学
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生物医学工程
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癌症研究
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神经退行性疾病
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DOI
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2020
·Vol. 72
·期 7
·969-978
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IF 2.571
Ding Ting,Yang Luo-Jie,Zhang Wei-Dong,Shen Yun-Heng
ObjectivesTo screen the cytotoxic activity of six secondary metabolites isolated from soil fungus Aspergillus niger. Importantly, to investigate the mechanism that pyoluteorin induced human triple-negative breast cancer MDA-MB-231 cells apoptosis in vitro.MethodsThe cell viability assay was tested with CTG assay. Cell cycle, apoptosis and intracellular reactive oxygen species (ROS) production assay were tested with flow cytometry. Additionally, intracellular ROS production assay and mitochondrial membrane potential assay were determined with laser scanning confocal microscopy. The expression of apoptosis-related proteins was determined with Western blot.Key findingsPyoluteorin displayed significantly selective cytotoxicity against human triple-negative breast cancer MDA-MB-231 cells (IC50 = 0.97 µm) with low toxicity against human breast epithelial cell MCF-10A. It was found that pyoluteorin could arrest MDA-MB-231 cells cycle at G2/M phase and induce cell apoptosis. Further experiments demonstrated that the apoptosis-inducing effect of pyoluteorin was related to reduction of mitochondrial membrane potential, accumulation of ROS and change of apoptosis-related protein expressions.ConclusionOur studies revealed that pyoluteorin had potent proliferation inhibition against MDA-MB-231 cells through arresting cell cycle at G2/M phase and inducing caspase-3-dependent apoptosis by mitochondrial pathway, implying that pyoluteorin may be a potential lead compound for drug discovery of human triple-negative breast cancer.
研究领域:
肿瘤学
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药理学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 15
·8862-8877
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IF 4.036
Yifan Wang,Huiqin Yao,Yan Zu,Wenyan Yin
Wounds infected with drug-resistant bacteria are hard to treat, which remains a serious problem in clinical practice. An innovative strategy for treating wound infections is thus imperative. Herein, we describe the construction of a nanocomposite from biocompatible poly(vinyl alcohol) (PVA)/polyethylene glycol (PEG) hydrogel loaded biodegradable MoOx nanoparticles (NPs) and photosensitizer methylene blue (MB), denoted as MoOx@MB-hy. By incorporating MoOx@MB NPs, the nanocomposite hydrogel can act as a photoactivated wound dressing for near-infrared-II 1064 nm and 660 nm laser synergetic photothermal–photodynamic therapy (PTT–PDT). The key to PTT-induced heat becomes the most controllable release of MB from MoOx@MB-hy to produce more 1O2 under 660 nm irradiation. Importantly, MoOx@MB-hy can consume glutathione (GSH) and trap bacteria nearer to the distance limit of ROS damage to achieve a self-migration-enhanced accumulation of reactive oxygen species (ROS), thereby conquering the intrinsic shortcomings of short diffusion distance and lifetime of ROS. Consequently, MoOx@MB-hy has high antibacterial efficiencies of 99.28% and 99.16% against Ampr E. coli and B. subtilis within 15 min. Moreover, the light-activated strategy can rapidly promote healing in wounds infected by drug-resistant bacteria. This work paves a way to design a novel nanocomposite hydrogel dressing for safe and highly-efficient antibacterial therapy.
研究领域:
材料科学
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纳米技术
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生物医学工程
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药学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Zhiren Zhou,Lina Zou,Ping Zhang,Chunmei Li,Ye Zhu,Huanyu Ren,Zheng Li,Huiru Niu,Hao Liao,Xiaojing Zhang,Shanshan An,Fei Ren,Xiuhong Ge,Lang Cheng,Feiyan Yang,Hongzhi Pan,Shengzhong Rong,Dong Chang,Hongkun Ma
Survivin serves as a crucial biomarker for bladder cancer, so exploring the detection method is essential. Therefore, a sensitive ratiometric electrochemical immunosensor was developed. In brief, a double-layered amino graphene oxide (NH 2 -GO) sandwich enhanced by Fe-Ni@Ti 3 C 2 T x (NH 2 -GO/Fe-Ni@Ti 3 C 2 T x /NH 2 -GO) significantly amplified the detection signal. ferrocene@Au nanoparticles (Fc@AuNPs) provided a reference signal, and Ab 1 was anchored via an Au-N bond. Moreover, AuPt-methylene blue (AuPt-MB) served not only as a secondary signal but also anchored Ab 2 through Au-NH 2 and Pt-NH 2 bonds. Black phosphorus nanoplates (BPNPs) improved conductivity and provided additional binding sites for AuPt-MB. The immunosensor exhibited a broad detection range (0.001~1000 ng/mL) and a low detection limit (0.00065 ng/mL), facilitating successful survivin detection in human urine samples with promising clinical analysis applications.
研究领域:
生物医学工程
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分析化学
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肿瘤学
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DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 4
·e202200523
·
IF 2.9
Zhonghua An,Xuelin Feng,Mengjia Sun,Yifan Wang,Hongbo Wang,Yanling Gong
Chamomile essential oil (CEO) is extracted from chamomile and mainly used in aromatherapy. The chemical constituents and its antitumor activity on Triple-negative breast cancer (TNBC) was explored in the present study. Gas chromatography-mass spectrometry (GC/MS) was employed to analyze the chemical constituents of CEO. The cell viability, migration and invasion of TNBC cell MDA-MB-231 were measured using MTT, wound scratch and Transwell assay, respectively. The protein expression of PI3K/Akt/mTOR signaling pathway was determined by Western blot. CEO is rich in terpenoids (63.51 %), among which the identified terpenoids and their derivatives are mainly Caryophyllene (29.57 %), d-Cadinene (12.81 %), Caryophyllene oxide (14.51 %), etc. Three concentration of CEO (1, 1.5, 2 μg/mL) significantly inhibited the proliferation, migration and invasion of MDA-MB-231 cells with a dose dependent manner. Moreover, the phosphorylation of PI3K, Akt and mTOR was inhibited by CEO. The results revealed that there was abundant terpenoids in the CEO which account for 63.51 %. CEO significantly inhibited the proliferation, migration and invasion of MDA-MB-231 cells, exhibiting antitumor effect on TNBC. The antitumor effect of CEO might attribute to its inhibition on PI3K/Akt/mTOR signaling pathway. However, further study should be conducted in more TNBC cell lines and animal models to provide further evidence for TNBC treatment by CEO.
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2024
·
IF 7.6
Lijie Wang,Li Wan,Jianxin Wu,Yeke Chen,Yuting Yang,Tian Deng,JingTing Wu,Weiwei Xue,Li Song,Fang Dai
The guided tissue regeneration (GTR) procedure has become an important process for the treatment of periodontal tissue regeneration, using the membrane as a mechanical barrier to generate a distance across the defect that subsequently allowing bone regeneration. Due to the complexity of periodontal microenvironment caused by bacterial infections, inflammation, and fibrous tissue penetration, the guided tissue regeneration membrane is required to have antibacterial and barrier functions. We developed a loaded methylene blue @ ZIF-8 and hydroxyapatite gradient thermoplastic polyurethane Janus membrane for antibacterial osteogenic and barrier. This Janus membrane with a porous gradient structure was prepared to use phase-conversion method. The rough layer facilitated cell growth and promoted periodontal bone regeneration, whereas the smooth layer isolated the growing fiber tissue.The rapid release of reactive oxygen species in the early stage and continuous release of zinc ions by methylene blue @ZIF-8 can achieve long-term synergistic antibacterial effect, with antibacterial rates of 84 % and 72 % against Escherichia coli and Staphylococcus aureus, respectively, which can effectively reduce the local inflammation caused by bacteria. In summary, we applied the Janus membrane to a rat periodontal bone defect model, and the membrane showed good surgical performance, barrier properties, osteogenic properties, and antibacterial effects, which could have broad application prospects in the treatment of periodontal bone defects.
研究领域:
生物材料
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组织工程
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牙科材料
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再生医学
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生物医学工程
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Xin Liu,Dawei Fan,Chenchen Zhang,Xinrong Shao,Hongmin Ma,Yong Zhang,Xueying Wang,Qin Wei,Huangxian Ju
An ultrasensitive photoelectrochemical (PEC) antifouling immunosensor was proposed using BiOBr/FeTPPCl/BiOI as matrix and polyacrylic acid (PAA)/polyethylene glycol (PEG) as hybrid antifouling interface for MDA-MB-231 detection. An indium tin oxide (ITO) electrode was successively modified with bismuth oxybromide (BiOBr) with layered crystal structure, which was sensitized with tetraphenyl iron(Ⅲ) porphyrin chloride (FeTPPCl) to widen the absorption range of visible light, and decorated with bismuth oxyiodide (BiOI) nanosheets to improve the PEC response. The joint action of PAA and PEG could offset the adsorption of non-specific proteins on the immunosensing interface for obtaining good antifouling performance. Based on the specific interaction of sialic acid (SA) on the cytomembrane with 3-aminophenylboronic acid (APBA), and the synergistic antifouling effect of PEG and PAA, a simple PEC immunosensing method was developed for the quantitative determination of MDA-MB-231 cells. This proposed antifouling PEC immunosensor exhibited a wide linear range (1 × 10 2 ∼ 1 × 10 6 cells·mL -1 ) and a low detection limit (30 cells·mL -1 ). The development of antibody-free immunosensors may improve the application for the diagnosis of human breast cancer.
研究领域:
材料科学
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分析化学
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生物化学
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癌症诊断
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产品关联
DOI
高影响因子
Cancer Medicine
·
2020
·Vol. 9
·期 7
·2564-2578
·
IF 3.491
Yubo Guo,Yingyi Fan,Xiaohua Pei
Triple-negative breast cancer (TNBC) is a basal-like cancer which is considered to be more intrusive, have a poorer prognosis and chemoresistance. TNBC is characterized by the presence of epithelial to mesenchymal transition (EMT) that plays a major role in the progression of the cancer. In the present study, we first use a classic prescription of Chinese medicine Fangjihuangqi Decoction to treat TGFβ1-induced MDA-MB-231 cells in vitro. Our data showed that TGFβ1-induced MDA-MB-231 cell morphology change, promoted MDA-MB 231 invasion, increased Vimentin expression, and decreased E-cadherin expression. Further, Fangjihuangqi Decoction-medicated serum (FHS) treated both MDA-MB 231 cells and TGFβ1-induced MDA-MB-231 cells. Results showed that Fangjihuangqi Decoction could inhibit cell proliferation, reduce cell invasion, increase E-cadherin expression, and decrease EMT markers. Secondly, we established a xenograft tumor zebrafish model to assess Fangjihuangqi Decoction inhibition of cancer cell proliferation and invasion. Our results indicated that Fangjihuangqi Decoction could inhibit tumor growth, restrain the sprouts number of tumor neovascularization, and reduce the length of tumor neoplastic lymphatics by increasing E-cadherin expression and decreasing EMT markers in TNBC xenograft tumor zebrafish model. Overall, our studies provide evidences that Fangjihuangqi Decoction could inhibit TNBC, reverse EMT, and contribute to antimetastasis by increasing E-cadherin expression and decreasing EMT markers, which provide an experimental basis for clinical application of Fangjihuangqi Decoction on TNBC treatment.
研究领域:
肿瘤学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
FIBERS AND POLYMERS
·
2025
·
IF 2.3
Zhang Dewei,Sun Hui,Yu Bin,Xie Youxiu,Li Fengchun
The removal of organic dyes from wastewater using manganese dioxide (MnO 2 ) and its composites has garnered significant attention, but recycling them remains challenging. In this study, MnO 2 was combined with polypropylene melt-blown nonwoven material (PP MB) in a simple way to prepare a composite material, which makes MnO 2 easy to be recycled and reused. MnO 2 with sea urchin-like structure were synthesized using a hydrothermal method and subsequently anchored onto the surface of PP MB modified by polydopamine (PDA) in various loading concentration, resulting in the preparation of MnO 2 @PP MB for removing organic dyes from wastewater. The size of the synthesized MnO 2 is about 6 μm and its specific surface area is 21.73 m 2 /g. When the loading concentration of MnO 2 is 1 mg/mL, the pH of adsorption solution is 3, and the adsorption solution temperature is 25 °C, the removal efficiencies of MnO 2 -1@PP MB for rhodamine B (RhB), methyl blue (MB), methyl orange (MO), and Carmine (CRM) in water all are achieved 99.00% in an impressive rate. Remarkably, even after undergoing twenty adsorption-desorption cycles, the removal efficiency for of MnO 2 -1@PP MB for RhB remained consistently around 99.00%. Furthermore, it was found that the removal behavior of MnO 2 @PP MB for RhB follows the pseudo-second-order kinetic model, meaning that is the adsorption process is primarily driven by chemical interactions. Compared to pristine PP MB, the mechanical properties of MnO 2 -1@PP MB just slightly decrease.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
FIBERS AND POLYMERS
·
2024
·
IF 2.2
Sun Yining,Sun Hui,Yu Bin,Yang Xiaodong,Xu Tao
Polypropylene melt-blown nonwoven material (PP MB) was first modified by polydopamine to improve the surface hydrophilicity. Then cobalt hydroxycarbonate Co(CO 3 ) 0.5 (OH)∙0.11H 2 O(CCO) was synthesized on the surface of the modified PP MB(M-PP MB) via a simple in suit hydrothermal method, and CCO@M-PP MB was obtained. The results showed that when the ratio of Co(NO 3 ) 2 ·6H 2 O to CO(NH 2 ) 2 was 1:5 and the Co(NO 3 ) 2 ·6H 2 O concentration was 0.03 mol/L, CCO with a sea urchin-like morphology appeared on the surface of M-PP MB. Compared with PP MB, the water contact angle (WCA) of CCO@M-PP MB reduced to 0°. When n-hexane was used as the oil phase, the underwater oil contact angle (UWOCA) of CCO@M-PP MB could be over 140.63°. CCO@M-PP MB could efficiently separate various oil–water mixtures, the oil included n-hexane, petroleum ether, carbon tetrachloride, dichloromethane, peanut oil, and olive oil. When n-hexane was used as the oil phase, the oil–water separation efficiency of CCO@M-PP MB was over 98.76%. Throughout 50 cycles, the oil–water separation efficiency of CCO@M-PP MB was never lower than 97.02%.
研究领域:
材料科学
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环境工程
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产品关联
DOI
高影响因子
LANGMUIR
·
2026
·
IF 4.4
Chunyi Li,Yuhe Xia,Xinyang He,Jiahui Zhu,Qi Feng,Hongjun Fu,Rongtai Yu
Methylene blue (MB), acid fuchsin (AF), and divalent copper ions (Cu2+) are common aquatic pollutants known for their environmental persistence and biotoxicity, posing considerable risks to ecosystem integrity and human health. Therefore, developing efficient adsorbents for their elimination is crucial. In this study, an asymmetric concave polymer was synthesized through a one-step self-assembly method employing 3-aminophenol (3-AP) as the monomer, formaldehyde as the cross-linker, and ammonia as the catalyst, and then subjected to etching with acetone to refine its concave structure. The polymer demonstrated outstanding synchronous adsorption performance in both Cu2+/AF and AF/MB binary systems, achieving maximum adsorption capacities of 473.5 mg/g for MB, 1250.6 mg/g for AF, and 988.64 mg/g for Cu2+. The adsorption behaviors of AF and Cu2+ conformed to the Langmuir model, implying monolayer, spontaneous, and endothermic chemisorption. While MB adsorption was more accurately represented by the Freundlich model, reflecting a heterogeneous surface-based, spontaneous, and endothermic process. The adsorption kinetics for all three contaminants were best described by the pseudo-second-order model, affirming chemisorption as the rate-controlling step. These results reveal that the asymmetric concave adsorbent functions through a synergistic mechanism involving electrostatic attraction, hydrogen bonding, and chemical complexation. This study underscores the material’s wide application potential in treating complex water pollutants.
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产品关联
DOI
PubMed
高影响因子
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