ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2014
·Vol. 406
·期 28
·7213-7220
·
IF 3.578
Gao Lin,Wang Jixiang,Li Xiuying,Yan Yongsheng,Li Chunxiang,Pan Jianming
In this study, we report here a general protocol for making core-shell magnetic Fe 3 O 4 /SiO 2 -MPS/MIPs (MPS = 3-(methacryloxyl) propyl trimethoxysilane, MIPs = molecularly imprinted polymers, Fe 3 O 4 /SiO 2 -MPS as core, MIPs as shell) via a surface molecular imprinting technique for optical detection of trace λ -cyhalothrin. The fluorescent molecularly imprinted polymer shell was first prepared by copolymerization of acrylamide with a small quantity of allyl fluorescein in the presence of λ -cyhalothrin to form recognition sites without doping. The magnetic Fe 3 O 4 /SiO 2 -MPS/MIPs exhibited paramagnetism, high fluorescence intensity, and highly selective recognition. Using fluorescence quenching as a detecting tool, Fe 3 O 4 /SiO 2 -MPS/MIPs were successfully applied to selectively and sensitively detect λ -cyhalothrin, and a linear relationship could be obtained covering a wide concentration range of 0–50 nM with a correlation coefficient of 0.9962 described by the Stern-Volmer equation. The experimental results of practical detection revealed that magnetic Fe 3 O 4 /SiO 2 -MPS/MIPs as an attractive recognition element was satisfactory for determination of trace λ -cyhalothrin in honey samples. This study, therefore, demonstrated the potential of MIPs for detection of λ -cyhalothrin in food.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Le Wu,Zheng-Zhong Lin,Jun Zeng,Hui-Ping Zhong,Xiao-Mei Chen,Zhi-Yong Huang
A magnetic fluorescent probe of CdTe QDs/nano-Fe 3 O 4 @MIPs was prepared using CdTe QDs and Fe 3 O 4 nanoparticles as co -nucleus and molecularly imprinted polymers (MIPs) as specific recognition sites based on a reverse microemulsion method. With the specific enrichment and magnetic separation properties, the probe of CdTe QDs/nano-Fe 3 O 4 @MIPs was used to detect malachite green (MG) in fish samples. The TEM analysis showed that the particles of CdTe QDs/nano-Fe 3 O 4 @MIPs were spherical with average diameter around 53 nm, and a core-shell structure was well-shaped with several Fe 3 O 4 nanoparticles and CdTe QDs embedded in each of the microsphere . Quick separation of the probes from solutions could be realized with a magnet, indicating the excellent magnetic property of CdTe QDs/nano-Fe 3 O 4 @MIPs. The probe exhibited high specific adsorption towards MG and excellent fluorescence emission at λ em 598 nm. The fluorescence of CdTe QDs/nano-Fe 3 O 4 @MIPs could be linearly quenched by MG at the concentrations from 0.025 to 1.5 μmol L −1 . The detection limit was 0.014 μmol L −1 . The average recovery of spiked MG in fish samples was 105.2%. The result demonstrated that the as-prepared CdTe QDs/nano-Fe 3 O 4 @MIPs could be used as a probe to the detection of trace MG in fish samples.
研究领域:
分析化学
·
食品安全
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 17
·1721-1729
·
IF 3.532
Di Deng,Xiaoyu Fang,Ding Duan,Kang Li
There is a critical need for the rapid detection of p-chlorophenol produced by pesticide abuse and industrial wastewater discharge, which has been an urgent problem in the realm of environmental protection. Here, a green and environmentally friendly method was developed to prepare stable and low toxicity quantum dots. First, blue-green fluorescent FeS2 quantum dots (B-FeS2 QDs) were prepared with FeCl3·6H2O (an iron source) and L-cysteine (a capping agent) by the solvothermal method. By combining B-FeS2 QDs with orange carbon dots (O-CDs), a CDs@SiO2/FeS2@MIPs visual fluorescence sensor for the selective detection of p-chlorophenol was constructed. Under optimum conditions, this sensor exhibited a detection limit of 1.265 μM with a linear range of 5.00–50.00 μM and was successfully applied to detect p-chlorophenol in real samples. Moreover, this sensor was successfully applied to visual semi-quantitative detection of p-chlorophenol. This work demonstrated that these sensors, based on FeS2 QDs and CDs, had potentials for in situ and visual detection of environmental contaminants.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR MODELING
·
2015
·Vol. 21
·期 11
·1-9
·
IF 1.736
Dai Zhengqiang,Liu Junbo,Tang Shanshan,Wang Yan,Wang Yiming,Jin Ruifa
Recently, a series of computational and combinatorial approaches were employed to improve the efficiency of screening for optimal molecularly imprinted polymer (MIP) systems. In the present work, we investigated MIP systems based on enrofloxacin (ENRO) as the template molecule and either 2-vinyl-4,6-diamino-1,3,5-triazine (VDAT), 4-vinylpyridine (4-Vpy), acrylamide (AM), or trifluoromethacrylic acid (TFMAA) as the functional monomer. The optimized geometries of these systems, the optimal molar ratios of template to functional monomer, and the active sites in the systems were all identified using density functional theory (DFT) at the B3LYP/6-31G( d , p ) level of theory. The imprinting mechanism was investigated by calculating the hydrogen nuclear magnetic resonance ( 1 H NMR) spectra of the systems. The simulated results revealed that the MIP system corresponding to a 1:7 complex of TFMAA and ENRO contained the most H-bonds and presented the lowest (i.e., most negative) binding energy and the strongest interactions. MIPs of ENRO with the four functional monomers were prepared based on the optimal molar ratios of template to functional monomer determined in the simulations. Adsorption experiments suggested that TFMAA has the highest affinity (saturated adsorption 30.25 mg/g) among the four monomers for the template. Thus, we determined the optimal monomer and imprinting ratio for ENRO-imprinted MIPs and predicted their adsorption characteristics.
The preparation and extraction processes of MIPs with ENRO as template, TFMAA as functional monomer, and EDMA as cross-linker
研究领域:
材料科学
·
化学工程
·
计算化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Yu Wu,Yuan Cong,Wenshi Zhao,Sicheng Cui,Boya Ma,Hanying Zhao,Yuhang Cao,He Liu,Yang Liu
Because of its broad antibacterial range, the fluoroquinolone antibiotic ciprofloxacin (CIP) has been used extensively to treat bacterial infections in humans. However, it has been demonstrated that the excessive use of CIP will endanger human health and destroy the ecological environment. Therefore, rapid, facile and accurate detection of CIP is essential for real-world applications. In this study, a novel surface-enhanced Raman scattering (SERS) sensor with self-cleaning ability was synthesized and successfully employed for ultra-sensitive and specific detection of CIP. Specifically, ZnFe 2 O 4 @SiO 2 -Au (ZSAu) nanocomposites (NCs) with different Au nanocrystals gap were developed. The relationship between various Au nanocrystals gap and SERS performance was investigated using finite-difference time-domain (FDTD) method. The solid-state Raman scattering spectrum of CIP molecule was also optimized and calculated using the density functional theory (DFT) method. After modifying with molecularly imprinted polymers (MIPs), ZSAu-MIPs NCs achieved specific detection of CIP in water environments, with the detection limit of low as 10 −9 M for CIP. A strong linear correlation between SERS intensity and the logarithm of CIP concentration suggested that ZSAu-MIPs NCs could be applied for quantitative detection of CIP at uncertain concentrations. In addition, ZSAu-MIPs NCs with self-cleaning ability could be recycled and reused with the assistance of magnet. After six cycles, ZSAu-MIPs NCs still exhibited good SERS performance. This magnetic MIPs-based SERS sensor offers a promising and recyclable method for ultra-sensitive detection of CIP in complex water environments.
研究领域:
环境科学
·
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Junkui Li,Chen Li,Wang Guo,Yiqing Guo,Xiaobo Zou,Zongbao Sun
Gutter oil, which poses a serious threat to human health and has generated widespread attention in recent years, is misused as cooking oil, creating a major food safety concern. Capsaicin has been identified as a promising indicator for the detection of gutter oil. Hence, in this study, we introduce a novel hybrid approach for capsaicin detection that combines magnetic molecular imprinting (MMIP) with surface-enhanced Raman scattering (SERS) technology to rapidly and accurately identify gutter oil. A magnetic molecularly imprinted polymer is developed with the incorporation of purified halloysite nanotubes, which provide a large specific surface area. The resulting product, magnetic halloysite molecularly imprinted polymers (MHNTs@MIPs), serves as a specific isolation material for the enrichment of capsaicin from complex matrices. The Ag NPs@Si (silver nanoparticles deposited on a silicon wafer surface) and Au NPs (gold nanoparticles), known for their excellent signal enhancement capabilities, work synergistically as the SERS substrate. Substantially enhanced SERS peaks are observed at 1173 cm −1 , indicating the potential of this method for capsaicin detection and gutter oil identification. A strong log-linear relationship between SERS intensity and the logarithm of capsaicin concentration is established across a wide range of 0.05–100 μM, with a detection limit of 67.7 nM. Furthermore, for both simulated and real gutter oil samples, capsaicin recovery rates of 95.5%–110.3% and 96.3%–104.9% were achieved using the SERS sensor, demonstrating good consistency with certified high-performance liquid chromatography (HPLC) results. Therefore, this SERS sensor can be served as efficient method for detecting capsaicin and monitoring oil quality.
研究领域:
食品安全
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Miaomiao Hu,Changchun Wen,Jian Liu,Minzhe Li,Nan Leng,Xiaohuan Guo,Qi Fang,Qinjie Kou,Rong Huang,Xiang-Cheng Lin
5-Hydroxyindole-3-acetic acid (5-HIAA) is a molecular marker that can be used in the early diagnosis of carcinoid tumors, and the development of sophisticated 5-HIAA assays is therefore of great importance. Surface-enhanced Raman spectroscopy (SERS) has been widely used for the rapid and sensitive detection of disease biomarkers. Insufficient specificity for tumor markers and poor spectral reproducibility are the bottlenecks in the practical use of SERS technology. In this study, based on MIL-125 surface-loaded gold nanoparticles (Au@MIL-125), a novel strategy was proposed to obtain Au@MIL-125@molecularly imprinted polymers (MIPs) as functional SERS substrates by wrapping a thin MIP shell around the Au@MIL-125 surface for selective separation followed by a 5-HIAA assay. The Raman peak intensity ratio (I 865 /I 1078 ) was used to quantify 5-HIAA after a SERS spectral calibration with an embedded internal standard (i.e., 4-aminobenzenethiol) to improve the quantitative accuracy. The linear range was from 10 −11 to 10 −7 M, and the limit of detection (LOD) was 5.45 × 10 −13 M. The method of integrating the MIPs with the metal MOF-based nanocomposites was shown to be useful in the analysis of real samples using SERS. The application of SERS for the selective and quantitative detection of analytes in real sample analysis, therefore, has great potential.
研究领域:
生物医学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ying Zhou,Donghui Wang,Dingnan Wang,Yang Wang,Yang Li,Jie Li,Yiming Zhang
Mimetic recognition materials for rapid and selective analysis of foodborne contamination from complex foodstuff becomes a hot research topic in the field of food safety. In this work, borate-affinity-functionalized MOF/molecularly imprinted polymer paper-based microfluidic chips (FSU-BA@MIP) were prepared by growing functionalized MOFs on paper surface with 3-carboxyphenylboronic acid (3-CPBA) as the ligands, followed by coating of a MIP layer of salbutamol (SAL) through a borate-affinity surface imprinting strategy. Using the obtained paper-based chip as a mimetic recognition and specific enrichment module, the fluorescence sensing platform achieved visual rapid quantitative analysis of SAL with a linear range of 0.1 × 10 −2 –5.0 mg L −1 . The limits of detection and quantification were calculated to be 0.095 and 0.290 μg kg −1 , respectively, and the sample loading via lateral flow aid could be completed within 30 min. The obtained MOFs/MIPs paper chips provide an effective way for the rapid analysis of harmful contaminants from different animal-derived foods.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2026
·Vol. 19
·期 8
·236
·
IF 3
Neng Jing,Yu Jimei,Guo Qian,Wang Yazhi,Wang Yan,Zhang Yilong,Chen Peng,Yang Kai
A molecularly imprinted polymer (MIP) coating for surface-enhanced Raman spectroscopy (SERS) was developed for the detection of sibutramine. Using sibutramine as the template, methyl methacrylate as the functional monomer, 1,4-butanediol dimethacrylate as the cross-linker, and phenylsilane as the initiator, MIPs were synthesized via molecular imprinting on silver nanorod–coated copper rods. The coating specifically recognizes sibutramine in complex matrices such as milk tea. The characteristic peak intensity is proportional to concentration over a linear range of 5–500 ng/mL, with a detection limit of 2 ng/mL. Recoveries for spiked samples ranged from 96.60 to 99.08% with a relative standard deviation of 2.64–8.67%, consistent with HPLC results. The coating exhibits dual functionality: It specifically recognizes and adsorbs sibutramine, and it is reusable. This work presents a novel SERS method for detecting sibutramine in diet foods, offering an accurate, low-cost, and highly selective approach for analyzing complex food systems.
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Pan Wang,Hengjia Zhu,Jinxin Liu,Yue Ma,Juntong Yao,Xiaohui Dai,Jianming Pan
The selective recognition, isolation, and subsequent enrichment of target glycoproteins are becoming more and more important in clinical diagnosis. In this work, nano-sized molecularly imprinted polymers integrated double affinity (i.e. metal ion affinity and boronate affinity) (D-MIPs) were prepared for specific separation of ovalbumin (OVA). Poly(styrene-glycidyl methacrylate) nanoparticles (PSG) with epoxy bonds were firstly grafted with iminodiacetic acid (IDA) by ring-opening reaction, and then the Cu 2+ was chelated to prepare PSG/IDA-Cu 2+ . Subsequently, imprinted OVA molecules were pre-immobilized through Cu 2+ ion affinity. Finally, a surface imprinting layer was formed though the gentle self-oxidization of a kind of boronic acid ligand (i.e. 3-aminophenylboronic acid, APBA). By washing out imprinted molecules, as-prepared D-MIPs posed higher monolayer binding capacity (138.92 mg g −1 ) and faster capture kinetics (30 min) for OVA, when compared with single affinity integrated MIPs (S-MIPs) and non-imprinted polymers (NIPs), and the chemical adsorption dominated double affinity was the rate-determining step for whole adsorption process. The imprinting factor (IF) of OVA, horseradish peroxidase (HRP) and bovine serum albumin (BSA) by the available D-MIPs were 4.44, 2.23 and 1.47, respectively, which confirmed the better selectivity for OVA. D-MIPs performed favorable regeneration ability and a promising application in actual sample. In addition, a combination of synergistic multiple bindings and imprinting effect, as the mechanism for enhancing specific separation, was also demonstrated.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2021
·Vol. 146
·期 12
·3924-3932
·
IF 4.616
Siyu Chen,Lianghui Luo,Lingyun Wang,Chunyan Chen,Hang Gong,Changqun Cai
Highly selective and highly efficient identification of large viruses has been a major obstacle in the field of virus detection. In this work, a novel sandwich resonance light scattering sensor was designed based on molecularly imprinted polymers (MIPs) and aptamers for the first time. One of the recognition probes was obtained by molecular imprinting using environmentally friendly carbon spheres as carriers and the other by modification of the aptamer that can specifically recognize hepatitis B virus (HBV) on the surface of silicon spheres. In the presence of both probes, an MIP-HBV-aptamer sandwich structure was formed continuously in the system with the increase in HBV concentration, resulting in a strong resonance light scattering response. Finally, satisfactory selectivity and sensitivity were obtained, and the imprinting factor was as high as 7.56, which was higher than that reported in previous works of viral molecular imprinting sensor. In addition, it is of great significance to solve the problem of insufficient selectivity of traditional detection methods for macromolecular targets.
研究领域:
分析化学
·
生物技术
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiao Wei,Guodan Xu,Chengcheng Gong,Fang Qin,Xinghui Gong,Chen Li
In this paper, we have presented a custom-tailored strategy to fabricate molecularly imprinted composite sensors (MIPs/QDs@SiO 2 ) with highly selective recognition ability for the determination of sulfanilamides (SAs). The modified SiO 2 sphere, modified QDs, acrylamide (AM), and ethyl glycol dimethacrylate (EGDMA) were used as the supporter, the fluorescent material, the functional monomer , and the cross-linker, respectively. Afterward, a two-step imprinting polymerization reaction was implemented to fabricate the novel sensor MIPs/QDs@SiO 2 . Attributing to the SiO 2 with the surface functional monomer and the QDs with functional group, SAs molecules are attracted to the QDs and bound to the SiO 2 surface, hence accelerating the growth of homogeneous recognition sites on the surface of SiO 2 and guaranteeing the distance between the recognition sites and the QDs. After the experimental conditions were optimized, such fluorescence sensors can be used for well selective detection of SAs with a linear range of 2.0–30 μmol/L and a limit of detection is 0.17 μmol/L. Most importantly, the MIPs/QDs@SiO 2 can be successfully applied to the determination of SAs in real river water, which demonstrate high selectivity and stability and offer a promising platform for detecting environmental pollutants.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）