INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2013
·Vol. 14
·期 4
·8603-8610
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IF 2.464
Hua Kuang,Gang Cui,Xiujin Chen,Honghong Yin,Qianqian Yong,Liguang Xu,Chifang Peng,Libing Wang,Chuanlai Xu
Simple immuno-magnetic separation tandem fluorescent probes based on quantum dots-antibody (QDs-Ab) were developed to detectSalmonellawith sensitivity of 500 cfu mL−1. With two monoclonal antibodies, which recognize different antigenic determinant on the surface ofSalmonella, we prepared antibody-coated magnetic nanoparticles (MNPs) and conjugates of QDs-Ab. The immune-magnetic beads were verified with high enrichment efficiency forSalmonella(90%). A sandwich structure formed if theSalmonellasolution was mixed together with immune-beads and QDs-Ab, and the fluorescent single from QDs was related to the amount ofSalmonella. A linear response between fluorescence intensity and various concentrations ofSalmonella(2.5 × 103to 1.95 × 108cfu mL−1) were observed with this proposed method. The total assay time forSalmonellawas 30 min, and no cross-reaction to other microbial strains, such asStaphylococcus aureus,Escherichia coli(E. coli) andEscherichia coli O157:H7(E. coli O157:H7), were found using this detection system. All our results showed that the simple homogeneous immunoassay could be applied inSalmonellascreening without time-consuming extra-enrichment of bacteria.Keywords:Salmonella;fluorescent immunoassay;magnetic separation;quantum dots
研究领域:
生物技术
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微生物学
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纳米技术
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食品安全
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2018
·
IF 8.173
Cong Li,Ruijun Wu,Juncheng Zou,Tingting Zhang,Sufang Zhang,Zhiquan Zhang,Xin Hu,Youqi Yan,Xiaomei Ling
Herein, the ternary composites, ultrasmall metal nanoparticles encapsulated in the anionic metal-organic frameworks/electrochemically reduced graphene oxide (MNPs@Y-1, 4-NDC-MOF/ERGO, M = Ag, Cu) are constructed by a cationic exchange strategy and an electrochemical reduction process for the electrochemical determination of H 2 O 2 . Both AgNPs@Y-1, 4-NDC-MOF/ERGO and CuNPs@Y-1, 4-NDC-MOF/ERGO display excellent electrocatalytic activity toward H 2 O 2 , but the former is superior to the latter. Such a difference in electrocatalytic activity can be explained by the characterization measurements, and the results manifest MNPs@Y-1, 4-NDC-MOF/ERGO (M = Ag, Cu) electrocatalysts have subequal MNPs sizes and electrochemical surface areas, but different electron transfer rate constants. The AgNPs@Y-1, 4-NDC-MOF/ERGO sensor shows a linear detection range from 4 to 11,000 μM with the detection limit of 0.18 μM. Moreover, MNPs@Y-1, 4-NDC-MOF/ERGO (M = Ag, Cu) exhibit excellent anti-interference performance and can be used for the detection of H 2 O 2 released from living cells. The proposed sensor takes full advantage of the catalytic property of MNPs, the size selectivity of Y-1, 4-NDC-MOF, and the fast electron transport effect of ERGO. Thus, the MNPs@Y-1, 4-NDC-MOF/ERGO/GCE (M = Ag, Cu) can be utilized to detect oxidase activities by monitoring H 2 O 2 produced in the presence of substrate and oxidase, and it is expected that composites with the molecular sieving effect and catalytic activity can be widely applied for catalysis, biomedicine , and biosensing fields.
研究领域:
生物传感
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电化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Hongyong Lin,Tengyang Liao,Yaoyao Sang,Jin Mao,Yu Liu,Bin Zhang,Di Sun,Wei Jiang
Highly accurate and sensitive detection of Vibrio parahaemolyticus is crucial for ensuring seafood safety and preventing the transmission of foodborne diseases. Herein, a dual-recognition strategy-driven colorimetric biosensor for the detection of V. parahaemolyticus was developed. This biosensor was based on the use of PEG-mediated cefepime-functionalized magnetic nanoparticles (Cefe-PEG-MNPs) and aptamer-modified Fe-doped polydopamine (Fe@PDA) nanozymes. The magnetic platform Cefe-PEG-MNPs was introduced to enhance the enrichment efficiency of V. parahaemolyticus , achieving a capture efficiency exceeding 90%. The Fe@PDA nanozyme exhibited notable peroxidase mimetic enzyme activity. The colorimetric biosensor developed for V. parahaemolyticus detection demonstrated good sensitivity, with a detection range spanning from 2.1 × 10 1 to 2.1 × 10 6 CFU/mL, and a limit of detection of 21 CFU/mL. Moreover, the biosensor demonstrated remarkable specificity, achieving good recovery rates from 95.80% to 103.80% in Penaeus vannamei with a relative standard deviation of less than 1.38%. The newly developed colorimetric biosensor offers a remarkable opportunity for the accurate and sensitive detection of V . parahaemolyticus in seafood.
研究领域:
食品安全
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微生物检测
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纳米技术
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生物传感
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2024
·Vol. 17
·期 1
·84-91
·
IF 2.7
Miaowen Xu,Gaoyang Chen,Yong Huang,Hangyu Song,Zheng Wu,Fengjuan Jiang,Lei Fu,Caili Bi,Xiaowei Cao,Wei Wei
This paper presents functionalized magnetic nanoparticles (Fe3O4@Au MNPs) combined with the surface-enhanced Raman spectroscopy (SERS) technique for sensitive detection of colorectal cancer (CRC) protein biomarker carbohydrate antigen 19-9 (CA19-9). Fe3O4@Au MNPs were constructed by the PEI-mediated seed growth method. Then, the signal molecule 5-5′-dithiobis (succinimidyl-2-nitrobenzoic acid) was used as a bridging agent to link CA19-9 antibody, and the SERS sensor was prepared. Using this sensor, detection of CA19-9 can be realized with only a one step sampling reaction. Fe3O4@Au MNPs combine the advantages of magnetic materials and noble metal nanoparticles, effectively amplifying the signal by creating numerous “hot spots” within the Au particle gaps and enhancing magnetic enrichment. Consequently, this approach lowers the detection limit (LOD) and enhances detection sensitivity. The ratio of characteristic peak intensities I1392/I1069 was selected for calculation, and a linear equation was constructed with a LOD as low as 0.27 U mL−1 by quantitative detections of the standard antigen. Finally, the sensor was used to analyze the clinical serum samples from CRC patients and healthy individuals, and the detection results were consistent with the actual results. This method exhibits notable advantages, including simplicity, high sensitivity, specificity, stability and reproducibility. It is expected to provide a new promising analytical method for clinical CA19-9 immunoassay.
研究领域:
生物医学工程
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肿瘤学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
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2014
·Vol. 4
·期 72
·38323-38333
·
IF 3.708
Jingwei Wan,Lu ding,Tao Wu,Xuebing Ma,Qian Tang
In this paper, a novel type of efficient, magnetically recoverable magnetic nanoparticles (MNPs)-supported 9-amino-9-deoxy-epicinchonidine organocatalysts were prepared through a facile co-precipitation method using phosphonic acid (–PO3H2) as an anchor point. These MNPs-supported organocatalysts possessed the high and tunable loading capacities of an organocatalyst (0.18–0.52 mmol g−1), 2–25 nm regular mesopores and 10.6–44.06 emu g−1 saturated magnetization. In the catalytic asymmetric aldol reactions of cyclohexanone with various o, m and p-substituted benzaldehydes in water, the aromatic aldehydes with electron-withdrawing substituents including-NO2, X and –CN afforded the relevant aldol adducts in excellent yields (86–100%) and steroselectivities (anti/syn = 82–98/18–2 and 93–98%ee anti). Moderate to good yields (36–97%) and steroselectivities (anti/syn = 82–96/18–4 and 75–97%ee anti) for the aromatic aldehydes with strong electron-donating substituents (–CH3 and –OCH3) were also satisfactorily achieved. Futhermore, these MNPs-supported organocatalysts could be quantitatively recovered from the reaction mixture using an external magnet, and reused six times with excellent catalytic performance (93%, anti/syn = 89/11 and 96%ee anti). Meanwhile, MNPs-supported organocatalysts, prepared by the surface-modification method, were investigated in detail as comparative samples.
研究领域:
化学
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催化材料
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有机合成
✓ 已验证
产品关联
DOI
高影响因子
Sensors and Actuators Reports
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2025
·
IF 7.6
Hao Liu,Hui Liang,Siyuan Zheng,Rui Hu,Wenqiang Pei,Le Wang,Yongchao Lai
Micro-nano plastics (MNPs) have become pervasive throughout the environment and emerged as significant pollutants, posing a potential threat to the natural environment and human health. Nonetheless, there remains a technological gap in the accurate analysis of trace and small-size MNPs in the environment. This study proposes a refined strategy for sample pretreatment and surface-enhanced Raman spectroscopy (SERS) analysis of MNPs in a water environment. MNPs were initially captured from the matrix using filter membranes, and subsequently released using an appropriate solvent. This approach not only separated the MNPs from the matrix and impurities, but also allowed the irregular and rigid MNPs to fit the surface of the KI-modified SERS substrates. This SERS-based approach facilitates the analysis of polystyrene (PS) MNPs of varying sizes at a concentration of 0.05 ng/mL across multiple water matrices. Furthermore, PS MNPs released from disposable cups were found using this method in everyday life. This simple and highly efficient strategy is ideal for the analyzing MNPs in everyday life and has the potential to facilitate future research on MNPs in the environment and their impact on human health.
研究领域:
环境科学
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分析化学
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纳米技术
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光谱学
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污染监测
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产品关联
DOI
高影响因子
Frontiers in Public Health
·
2022
·
IF 6.461
Shi Xuening,Sun Hongbin,Li Hang,Wei Shengnan,Jin Jin,Zhao Chao,Wang Juan,Li Hui
Immunomagnetic separation based on Fe3O4 magnetic nanoparticles (MNPs) has been widely performed in sample pretreatment. The oriented conjugation strategy can achieve a better capture effect than the N-(3-dimethylamlnopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) /N-hydroxysuccinimide (NHS) method. However, immunoglobulin yolk (IgY) cannot be oriented through an SPA strategy like immunoglobulin G (IgG). In this article, an oriented conjugation nanoprobe was prepared for the enrichment of bacteria based on pH adjusting. The main factors affecting the enrichment efficiency were studied, such as the pH of the buffer system, the concentration of IgY, the concentration of nanoprobe, and the enrichment time. Under the optimal conditions, the enrichment efficiency toward target bacteria could reach 92.8%. Combined with PCR, the limit of detection (LOD) was found to be 103 CFU/ml, which was lower than the PCR only. In conclusion, we provided a new protocol for the oriented conjugation of IgY and high sensitivity detection with simple pretreatment.
研究领域:
生物医学工程
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微生物学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Nanomaterials
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2018
·
IF 2.207
Kun Wang,Shouju Wang,Kun Chen,Yijing Zhao,Xingqun Ma,Lin Wang
Anaplastic thyroid cancer (ATC) is highly aggressive and tends to develop drug resistance to standard chemotherapies. To overcome the drug resistance of ATC, we synthesized dopamine-melanin nanoparticles (MNPs) loaded with doxorubicin (Dox) molecules. The Dox-loaded MNPs (Dox-MNPs) developed exhibited increased cellular uptake and enhanced therapeutic efficacy in drug-resistant ATC cell line HTh74R, compared with free Dox. The native MNPs were found to have excellent biocompatibility, which suggests that Dox-MNPs may have potential in the treatment of ATC.
研究领域:
肿瘤学
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药学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
·
IF 5.2
Chenchen Zhu,Shuimiao Wang,Doudou Wang,Mengxia Hua,Sen Lin,Baoshan Huang,Shengjin Xiang,Kaihui Nan
Ciliary neurotrophic factor (CNTF) shows potential for stimulating axon regeneration of retinal ganglion cells (RGCs), whereas unordered regeneration of axons does not have any biological significance. Given the chemotaxis characteristics of RGCs, materials with gradient CNTF distribution can chemically stimulate axon extension based on the CNTF concentration gradient. Therefore, a novel nano-micro-macro multiple-nested hydrogel with gradient CNTF distribution was developed in this study to induce directional axonal outgrowth of RGCs. The CNTF and magnetic nanoparticles (MNPs) were co-loaded into gelatin microspheres (CNTF& [email protected] ), and subsequently suspended in a gelatin methacryloyl (GelMA) solution (viscous). Using a magnet, the CNTF& [email protected] were moved from the far-magnet side toward the near-magnet side forming gradient CNTF& [email protected] distribution inside the GelMA solution. The GelMA was then cross-linked via ultraviolet (365 nm) to form a hydrogel with gradient CNTF distribution. This hydrogel was further applied as the matrix for retinal explant culturing. The results showed that the number of axons in the segments of 200–300 µm and > 300 µm was 10.21 times and 15.02 times of those in the Gradient than those in the Without Gradient. The axons area distribution was 3.28 times and 13.41 times, respectively. 86.1% of the axons area in the Gradient were distributed in the segments > 200 µm, whereas only 56.27% in the Without Gradient. The results suggested that the hydrogel-matrix with gradient CNTF distribution could intensely induce directional axonal extension of RGCs in the retinal explant. The present study provides a new method for CNTF distribution, which could induce directional axonal regeneration of RGCs, and in turn have potential application in optic nerve injury repair to some extent.
研究领域:
神经科学
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生物材料
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组织工程
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再生医学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Sun Xiaolong,Hu Jiahuan,Wang Yifeng,Luo Xi,Huang He,Fu Yongqian
The main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes via one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N2 sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid and alkali, temperature and organic solvents. Furthermore, the bio-catalyst could be separated easily using a magnet, and the reusability was once considerably expanded with 80% of enzyme activity last after eight rounds of recycling. Therefore, LDH/MNPs@MAF-7 could be used as a potential biocatalyst for the biosynthesis of D-PLA due to its good stability and recovery properties.
研究领域:
生物技术
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催化
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材料科学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Mingxia Jiang,Yuyin Lin,Liyuan Xu,Zhihang Chen,Junxian Wu,Yong Yuan,Lihua Zhou
Extracellular respiratory bacteria (ERB) assume a significant role in biological and environmental remediation, so the rapid detection of ERB is essential to monitor its biological repair process. This study developed a microfluidic colorimetric biosensor for rapid and sensitive detection of ERB model strain Shewanella oneidensis MR-1. In the process of separation and enrichment of bacteria based on immune magnetic nanoparticles (MNPs), the biological signal is significantly amplified by the excellent peroxidase activity of flower-like CoOOH (FL-CoOOH). Firstly, immune MNPs, samples and immune FL-CoOOH were mixed and incubated within the microfluidic chip’s channel. Magnetic capture in the separation chamber yielded MNP-bacteria-CoOOH complex. Then, 3, 3′, 5, 5′-tetramethylbenzidine (TMB) substrate was added to make the FL-CoOOH nanozyme catalyze the formation of yellow product. Finally, the target bacteria in the microplate were quantitatively detected. Based on the excellent simulated enzyme activity and stability of FL-CoOOH, the detection can be completed within 45 min after the introduction of the microfluidic chip. The detection was 5×10 1 ∼5×10 6 CFU/mL. Compared with the previously reported colorimetric sensors for MR-1, the sensitivity was increased by 99.54 % to 23 CFU/mL. This study uniquely integrates optical sensors and microfluidic technology for rapid ERB detection with enhanced portability. The design of biosensor can facilitate further investigations into extracellular respiration mechanisms and rapid ecological environment restoration applications.
研究领域:
生物技术
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环境科学
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分析化学
·
微流控
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2024
·
IF 1.3
Xingyuan Guo,Fang Li,Xueying Zhu,Pengying Zhang,Yunhong Liu,Zhaozhou Li
Enrofloxacin (ENR) is widely applied in preventing and treating animal diseases and hence directly passed into animal-derived foods. Therefore, the detection of this drug in food samples must be strengthened to ensure food safety. In this work, a simple, sensitive electrochemical aptasensor for the detection of ENR was fabricated by using magnetic nanoparticles (MNPs) as carriers. The ENR aptamer was modified on the surface of MNPs to capture the ENR target or a biotin-complementary strand and then recognize streptavidin-horse radish peroxidase. Subsequently, the resulting MNPs were easily transferred onto the surface of magnetic glassy carbon electrode with the help of its integral magnet. Under optimal experimental conditions, a linear relationship between the variable of LSV current and the logarithm of ENR concentration in the range of 0.01–100 ng/mL was obtained with the limit of detection at 4.9 pg/mL. In addition, this method exhibited good stability, selectivity, reproducibility and was successfully applied in detecting ENR in spiked chicken samples with recoveries of 100.70%–108.96%. The proposed method is easy to fabricate and process; it is particularly suitable for food sample pretreatment and is beneficial in removing the interference of a complex matrix.
研究领域:
分析化学
·
食品科学
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纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）