FOOD CHEMISTRY
·
2026
·
IF 10.4
Qijun He,Wei Hu,Zhihao Yi,Bin Du,Yuan He,Jianjie Xu
Early and portable detection of foodborne pathogens is a key challenge for food safety, as conventional methods suffer from insufficient sensitivity and cumbersome operation. Herein, inspired by cellular compartmentalization, we designed the composite nanozyme MIL-88(Fe)@Pt@COF-B(OH) 2 (MPC-B(OH) 2 ) and constructed a dual-mode biosensing platform with Escherichia coli ( E. coli ) as the model. In this design, a catalytic MIL-88(Fe)@Pt core was encapsulated by a boronic acid-functionalized COF shell, which mimicked a cell membrane to provide specific recognition while optimizing the local microenvironment to enhance peroxidase-like activity, as clarified by density functional theory calculations. Meanwhile, its porous COF layer improved electrochemical performance. This sensor achieved detection limits of 2 and 3 CFU/mL for colorimetric/electrochemical modes, and 7 CFU/mL for on-site detection via a smartphone platform. With excellent selectivity, stability and reliability in real samples, this work provided a new strategy for biomimetic nanozyme design and a practical platform for on-site food safety monitoring.
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高影响因子
LANGMUIR
·
2018
·
IF 3.789
Yaoyu Jiao,Sizhe Liu,Yulong Sun,Wen Yue,Hongyu Zhang
The addition of nanoparticles to water-based lubricants is a commonly used method to improve lubrication, but to the best of our knowledge few studies have been reported to investigate the lubrication property of surface-modified nanodiamonds (ND) with polyzwitterionic brushes. In this study, a bioinspired copolymer containing dopamine and 2-methacryloyloxyethyl phosphorylcholine (MPC) was synthesized (DMA-MPC) and then spontaneously grafted onto the ND surface (ND-MPC) through simple stirring in order to enhance lubrication. The characterization of transmission electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis indicated that the DMA-MPC was successfully modified on the ND surface. Furthermore, a series of tribological experiment were performed on a universal materials tester using glycerol, glycerol + ND, and glycerol + ND-MPC as the lubricants. It was found that the addition of ND to the lubricant (i.e., glycerol + ND and glycerol + ND-MPC) significantly reduced wear with a smaller wear scar and wear track on the tribopairs, and the coefficient of friction further decreased by about 40% when using glycerol + ND-MPC as the lubricant, which could be attributed to the hydration lubrication of the polyzwitterionic brushes modified on the ND surface and the rolling effect of nanoparticles. In conclusion, in this study a universal and versatile surface modification method was proposed on the basis of the synthesis of bioinspired copolymer DMA-MPC, which remarkably enhanced the lubrication property of ND nanoparticles when added to water-based lubricants.
研究领域:
材料科学
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纳米技术
·
摩擦学
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DOI
高影响因子
RSC Advances
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2018
·Vol. 8
·期 63
·36331-36337
·
IF 2.936
Huishang Yang,Shenggui Chen,Lei Liu,Chen Lai,Xuetao Shi
Phosphorylated biopolymers can induce mineralization, mimic the process of natural bone formation, and have the potential as scaffolds for bone tissue engineering. 2-Methacryloyloxyethyl phosphorylcholine (MPC), a low cytotoxicity phosphorus source, is mainly applied in vascularization and promoting blood compatibility and has been less researched for bone repair. In this study, phosphorylated methacrylamide chitosan (PMAC) hydrogel was prepared by mixing methacrylamide chitosan (MAC) and different mass of MPC with photoinitiator under UV irradiation. A series characterization tests showed that PMAC hydrogels were successful prepared and had a pretty good mineralization ability. Moreover, human fetal osteoblastic (hFOB) cells cultured on PMAC hydrogels exhibited not only highly viability but also the enhanced ALP activity and calcium deposition. The PMAC hydrogels have great potential in bone tissue engineering applications.
研究领域:
材料科学
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生物医学工程
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组织工程
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PubMed
高影响因子
LANGMUIR
·
2019
·
IF 3.683
Sizhe Liu,Qian Zhang,Ying Han,Yulong Sun,Yifei Zhang,Hongyu Zhang
In clinics it is extremely important for implanted devices to achieve the property of enhanced lubrication and bacterial resistance; however, such a strategy has rarely been reported in previous literature. In the present study, a surface functionalization method, motivated by articular cartilage-inspired superlubrication and mussel-inspired adhesion, was proposed to modify titanium alloy (Ti6Al4V) using the copolymer (DMA–MPC) synthesized via free radical copolymerization. The copolymer-coated Ti6Al4V (Ti6Al4V@DMA–MPC) was evaluated by X-ray photoelectron spectroscopy, water contact angle, and Raman spectra to confirm that the DMA–MPC copolymer was successfully coated onto the Ti6Al4V substrate. In addition, the tribological test, with the polystyrene microsphere and Ti6Al4V or Ti6Al4V@DMA–MPC as the tribopair, indicated that the friction coefficient was greatly reduced for Ti6Al4V@DMA–MPC. Furthermore, the bacterial resistance test showed that bacterial attachment was significantly inhibited for Ti6Al4V@DMA–MPC for the three types of bacteria tested. The enhanced lubrication and bacterial resistance of Ti6Al4V@DMA–MPC was due to the tenacious hydration shell formed surrounding the zwitterionic charges in the phosphorylcholine group of the DMA–MPC copolymer. In summary, a bioinspired surface functionalization strategy is developed in this study, which can act as a universal and promising method to achieve enhanced lubrication and bacterial resistance for biomedical implants.
研究领域:
材料科学
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生物医学工程
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表面化学
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摩擦学
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生物材料
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Luyan Li,Hao Li,Mingzhang Lin,Ning Wang,Sheng Hu,Jun Wen
Developing efficient uranium adsorbent remains a considerable challenge owing to the complexity of seawater and wastewater systems. Herein, a novel graphene oxide (GO)/chitosan (CS)/2-methacryloyloxyethyl phosphorylcholine (MPC) (GCM) aerogel, similar to a turbine with multiple ‘blades’ in appearance, was prepared for separating and extracting uranium. The highly vertically aligned lamellar structure with microchannels provides sufficient movement space for uranium, and the introduction of GO and MPC endows GCM aerogel with excellent photothermal conversion performance, strong uranium adsorption selectivity and biofouling resistance activity. GCM aerogel possessed an adhesion resistance rate of up to 99 % against marine bacteria, and its saturation adsorption capacity of uranium reached 1912 mg/g. It also exhibited good uranium selective adsorption capacity in natural seawater owing to its high adsorption capacity, remarkable selectivity, and outstanding biofouling resistance activity. Additionally, the one-piece GCM aerogel column served as an effective column separation material to maximize the photothermal conversion characteristics. The adsorption capacity and adsorption rate of GCM aerogel column for uranium were significantly enhanced and the recovery rate of uranium from simulated uranium-containing wastewater reached 95.3 % under simulated sunlight irradiation. This study offers a novel strategy for separating and extracting uranium from seawater and wastewater.
研究领域:
环境科学
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材料科学
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化学工程
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高影响因子
LANGMUIR
·
2024
·
IF 3.7
Yiran Jia,Yinuo Yang,Hongyu Zhang
Surface modification of lubricating coatings on biomedical devices is a pivotal strategy to improve the overall performance and clinical efficacy, significantly reducing friction between devices and human tissues and mitigating tissue damage during intervention and long-term implantation. Recently, various hydrophilic polymeric materials have been used for achieving surface functionalization, endowing the biomedical device with excellent superlubrication performance. N-Vinylpyrrolidone (NVP) and 2-methacryloyloxyethyl phosphorylcholine (MPC) are two typical representatives of nonionic and zwitterionic materials. However, there is still a research gap in a comparative study of the lubrication mechanisms and properties between them. In this study, a bioinspired and dopamine-assisted codeposition technique was used to fabricate biomimetic hydrophilic coatings, including P(DMA-NVP) and P(DMA-MPC), on polyurethane. To achieve a thorough comparative analysis of the self-adhesive coating performance, 3 M ratios of the copolymers were synthesized and comprehensive material evaluations were conducted. Additionally, surface morphology, hydrophilicity, and lubrication at both the microscale and macroscale were performed. It was found that both hydrophilic coatings exhibited good stability. The P(DMA-MPC) coating, due to the ability to attract and bind a large number of water molecules, demonstrated superior lubrication effects compared to the P(DMA-NVP) coating. The study provides an in-depth understanding of the lubrication behavior of the self-adhesive coatings to enhance the functionality and application in biomedical engineering.
研究领域:
生物医学工程
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材料科学
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表面化学
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高影响因子
MICROCHIMICA ACTA
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2019
·Vol. 186
·期 12
·1-8
·
IF 5.479
Yi Xiaotong,Liu Chang,Liu Xueke,Wang Peng,Zhou Zhiqiang,Liu Donghui
Magnetic partially carbonized cellulose nanocrystals (MPC-CNC) were obtained by sulfuric acid treatment of microcrystalline cellulose (MCC) and then loaded with magnetic Fe 3 O 4 nanoparticles. The material is shown to be a viable material for magnetic solid phase extraction of triazine and triazole pesticides from water. The pesticides (specifically: simazine, ametryn, prometryn, terbutryn, atrazine, triadimenol, epoxiconazole, myclobutanil, triadimefon and tebuconazole) were quantified by ultra HPLC in tandem with a triple quadrupole mass spectrometry (UHPLC-MS/MS). The effects of NaCl concentration, amount of adsorbent, vortex time, sample volume and pH value on extraction efficiency were optimized by Plackett-Burman design and Box-Behnken design methods. Under the optimal conditions, the method shows the following figures of merit: (a) Linear responses in the range from 0.02–10 μg L −1 ; (b) detection limits between 2.2 to 6.1 ng L −1 (for S/ N = 3); (c) recoveries from spiked samples of 73.7–117.1% with relative standard deviations (RSDs) of 2.0–15.7%; and (d) an enrichment factor of 75. The method was successfully applied to the determination of the pesticides in five environmental water samples.
Schematic representation of the process of magnetic solid phase extracting pesticides in water using MPC-CNC. MCC-microcrystalline cellulose; PC-CNC- partially carbonized cellulose nanocrystals; MPC-CNC-magnetic partially carbonized cellulose nanocrystals.
研究领域:
环境科学
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分析化学
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纳米技术
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水质分析
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高影响因子
ACS Applied Polymer Materials
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2022
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IF 4.855
Lei Cheng,Chao Liu,Ji Wang,Yang Wang,Wenhui Zha,Xinsong Li
Silicone rubber catheters have a wide range of medical applications. However, silicone rubber is subject to biofouling, biofilm formation, and microbial infection, which can decrease its success and performance because of lipophilicity. In this report, a tough hydrogel was grafted onto the surface of silicone rubber to endow it with antifouling and antibacterial properties. First of all, an acrylamide (AAm)-, 2-methacryloxyethyl phosphorylcholine (MPC)-, and zinc methacrylate (ZMA)-derived poly(AAm-MPC-ZMA) hydrogel was coated on the surface of silicone rubber mediated by a poly(vinyl acetate) (PVAc) primer layer. Second, the composition and hydrophilicity of the hydrogel-coated rubber were evaluated by X-ray photoelectron spectroscopy (XPS) and a contact angle analyzer. The results indicated the success of the poly(AAm-MPC-ZMA) hydrogel coating, inducing a decrease in the contact angle from 107.01 to 23.22°. Bovine serum albumin (BSA) was further used to test the nonspecific protein absorption of the modified silicone rubber. It was found that BSA deposition on the surface of silicone rubber decreased from 0.33 to 0.07 mg/cm2 after coating, indicating an antifouling effect. In addition, the incorporation of ZMA gave silicone rubber significant antibacterial properties against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. As a preliminary application test, a silicone rubber catheter was coated with the poly(AAm-MPC-ZMA) hydrogel, which showed improved hydrophilicity and reduced adhesion against biological soft tissues. Taken together, the poly(AAm-MPC-ZMA) hydrogel coating generated a highly hydrophilic silicone rubber with excellent antifouling and antibacterial properties, which has great potential in the application of medical catheters.
研究领域:
材料科学
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生物医学工程
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表面化学
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Materials Today Communications
·
2021
·
IF 3.383
Lijing Dai,Meiying Liu,Wei Long,Xin Hu,Hui Ouyang,Yulin Feng,Fengjie Deng,Yuanqing Wen,Xiaoyong Zhang,Yen Wei
As a member of nano-carbon compound, nanodiamonds (ND) with diverse structure, small size and relative low toxicity has been extensively explored for different applications especially as delivery vehicles for drugs. However, surface functionalization of ND with polymers is still required to overcome their poor water dispersibility and improve drug delivery performance. In this work, we reported a simple photocatalytic atom transfer radical polymerization (ATRP) method for surface functionalization of ND by using FeBr 3 as the photoredox catalyst. Owing to the introduction of water dispersible 2-methacryloyloxyethyl phosphorylcholine (MPC), resultant NDs-MPC displayed improved water dispersibility and low toxicity. The anticancer agent DOX could be loaded on NDs-MPC efficiently and controlled release from ND-P-DOX complexes. The cell viability and cell imaging results demonstrated that DOX can be transported into cells and maintained its corresponding therapeutic effect. More importantly, as compared with traditional ATRP, photocatalytic ATRP could not only operate under rather mild conditions but also could avoid utilization of expensive and toxic agents. Considered the advantages of photocatalytic ATRP and ND, the method developed in this work could be a promising strategy for fabrication of multifunctional ND-based composites for different applications.
研究领域:
纳米技术
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药学
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材料科学
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生物医学工程
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jianguo Chang,Nailong Gao,Peng Dai,Ziming Zhu,Hui You,Wei Han,Lu Li
The powerful immunoassay has attracted increasing attention for detecting trace substances. Herein, based on the co-coupling of phenylboronic acid and Protein A for oriented antibodies immobilization, we proposed a polymeric device-based fluorescence immunoassay with enhanced assay performance for Alpha-fetoprotein (AFP) analysis. Unlike traditional physical absorption or chemical covalent conjugation to immobilize antibodies, in this immunoassay, the bioreactivity of AFP antibody was maximumly obtained owing to the oriented immobilization manner. The package density of AFP antibody on the polymethylmethacrylate (PMMA) surface is significantly improved because of the co-coupling case associated distance effect. In addition, the non-specific absorption is greatly inhibited with the combined using of zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) and bovine serum albumin (BSA). These integrated features facilitate the output of strong fluorescence in the presence of AFP but emit very weak background in the absence of AFP. After a typical sandwich immunoreaction, the recorded fluorescence intensity of the immunosensor is proportional to the AFP concentration, showing excellent sensitivity, specificity, and reliability for AFP determination. Comparative studies show the co-coupling is superior than its single coupling counterparts. We envision this unique immunoassay can provide a new avenue for building immunosensors in the fields of disease diagnosis, food safety detection, and environmental monitoring.
研究领域:
生物医学工程
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分析化学
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医学诊断
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2022
·
IF 10.319
Feng Guo,Rizheng Han,Jishan Ying,Zeping Zhang,Rui Yang,Xing Zhang
In this work, bioinspired anisotropic polymeric heart valves were fabricated using composite materials from polyurethane (PU) and natural cellulose fiber bundles. Cellulose fibers with good alignment were obtained from balsa wood by a top-down process, which were then distributed in polyurethane to prepare cellulose fiber bundles reinforced polyurethane (CPU) by hot pressing. The storage modulus of the CPU along the direction parallel to the fiber alignment was 16.70 ± 0.80 MPa, whereas that along the direction perpendicular to the fiber alignment was 8.41 ± 0.94 MPa by dynamic mechanical analysis (DMA) tests at 1 Hz, comparable to aortic valve leaflets. Moreover, 2-methacryloyloxyethyl phosphorylcholine (MPC) was grafted onto the CPU surface (CPU-MPC) to improve hemocompatibility. With MPC modification, the water contact angle decreased significantly from 54.58° ± 2.98° to 26.42° ± 3.50°, and the platelet adhesion was reduced by 92%, compared to the original CPU. In vitro cell culture proved that both CPU and CPU-MPC samples did not show any cytotoxicity. Furthermore, the CPU composites were used to fabricate polymeric heart valves, which showed excellent hydrodynamic performance with a large orifice area (1.70 cm 2 ) and low regurgitation fraction (0.7%), meeting the requirements of ISO 5840–2 standard.
研究领域:
材料科学
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生物医学工程
·
生物材料
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高影响因子
PHYTOMEDICINE
·
2023
·
IF 7.9
MinChao Feng,BaiJun Qin,Fang Luo,Hui Li,KunRong Liu,Kai Li,DongYang Wu,XiPing Tang,Guozhong Chen
Background Safer and more effective drugs are needed for the treatment of acute pancreatitis (AP). Qingjie Huagong decoction (QJHGD) has been applied to treat AP for many years and has shown good clinical effects. However, the potential mechanism has not yet been determined. Purpose To investigate the role and underlying mechanism of the effects of QJHGD on AP both in vitro and in vivo . Methods QJHGD was characterized by UHPLC-Q-Orbitrap-MS. The protective effect of QJHDG and the underlying mechanism were investigated in MPC-83 cells in vitro . A caerulein-induced AP model was established to evaluate the protective effect of QJHGD in mice. CCK-8 assays were used to detect cell viability. The contents of inflammatory mediators were determined by ELISA. Expression levels of circRNA, miRNA and mRNA were determined by qRT-PCR. Protein expression was determined using Western blot . Pancreatic tissues were assessed by hematoxylin and eosin staining as well as immunohistochemical and immunofluorescence analyses. Pull-down and luciferase activity assays were performed to determine the regulatory relationships of circHipk3, miR-193a-5p and NLRP3. Results Our results confirmed that mmu-miR-193a-5p was sponged by mmu-circHipk3, and NLRP3 was a target of miR-193a-5p. In vitro experiments showed that QJHGD enhanced MPC-83 cell viability by regulating circHipk3 sponging mir-193a-5 targeting NLRP3 and inhibiting pyroptosis-related factors. Finally, we showed that QJHGD ameliorated pancreatic tissue injury in AP mice via this pathway. Conclusion This study demonstrate that QJHDG exerted its anti-AP effects via the circHipk3/miR-193a-5p/NLRP3 pathway, revealing a novel mechanism for the therapeutic effect of QJHDG on AP.
研究领域:
药理学
·
细胞生物学
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分子生物学
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炎症
·
中医药
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PubMed
高影响因子
危险品化学品经营许可证（带存储）