OPTICS EXPRESS
·
2025
·Vol. 33
·期 19
·40647-40660
·
IF 3.3
Hongtao Li,Hongtao Li,Hongtao Li,Hongtao Li,Jiaoyan Dai,Jiaoyan Dai,Jiaoyan Dai,Weisheng Wang,Weisheng Wang,Weisheng Wang,Yuxuan Liu,Yuxuan Liu,Yuxuan Liu,Lei Huang,Lei Huang,Liang Lu,Liang Lu,Liang Lu,Jialiang Lv,Jialiang Lv,Jiping Lin,Jiping Lin,Jiping Lin,Lili Liang,Lili Liang,Benli Yu,Benli Yu,Benli Yu
For efficiently diagnosing early-stage breast cancer, current existing MUC1 biomarker detection methods suffer from common issues of low sensing sensitivities. In this study, a single-core two-channel optical fiber optofluidic on-chip sensor is employed to high-sensitivity detect breast cancer biomarker MUC1 protein, where a Mach-Zehnder interferometer (MZI) waveguide and a fiber Bragg grating (FBG) inscribed into the device by using a femtosecond laser direct writing technique. To improve the device’s sensitivity, two microfluidic channels are etched using hydrofluoric (HF) solution, and Au nanobipyramids (Au NBPs) material on an optical fiber is used to excite and realize the localized surface plasmon resonance (LSPR) enhancement. Thus, the device’s refractive index (RI) sensitivity increases from 25.07 to 580.22 nm/RIU. FBG compensates the device’s temperature sensitivity, achieving at -0.139 nm/℃. On the basis of the advanced LSPR enhancement strategy, the sensor can not only specifically sense MUC1 protein with an ultra-low limit of detection (LOD) of 20.74 aM, it can also detect MUC1 protein in real serum background with a LOD of 42.07 aM. The obtained ultra-low LOD is lower than most existing state-of-the-art MUC1 detection technologies. This study paves what we believe to be a new universal way for developing advanced high-sensitivity lab-in-fiber optofluidic sensing strategy.
研究领域:
肿瘤学
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生物医学工程
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分子诊断
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光学传感
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转化医学
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纳米生物技术
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液体活检
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DOI
PubMed
高影响因子
LABORATORY INVESTIGATION
·
2018
·Vol. 98
·期 12
·1642-1656
·
IF 4.263
Cao Zhanqi,Hao Zhaojun,Xin Ming,Yu Lugang,Wang Lei,Zhang Ying,Zhang Xinke,Guo Xiuli
Tumor cell-endothelial adhesion is one of the key steps in tumor cell haematogenous dissemination in metastasis and was previously shown to be mediated by interaction of galectin-3 with the transmembrane mucin protein MUC1. In this study, the effect of exogenous as well as endogenous galectin-3 on adhesion of two cell lines (low MUC1-expressing human prostate cancer PC-3M cells and non-small-cell lung cancer A549 cells) to monolayer of umbilical vein endothelial cells (HUVECs) was investigated. We found that suppression of endogenous galectin-3 expression reduced tumor cell adhesion to HUVECs and also decreased cell invasion and migration. Exogenous galectin-3 promoted tumor cell adhesion to HUVECs by entering cells. Both exogenous and endogenous galectin-3 upregulated the expression of β-catenin and increased β-catenin nuclear accumulation, and subsequently upregulated the expression of N-cadherin and CD44. We deduced that both exogenous as well as endogenous galectin-3 promoted low MUC1-expressing cancer cell adhesion to HUVECs by increasing the expression of N-cadherin and CD44 via an increase of nuclear β-catenin accumulation. These results were confirmed further by using a β-catenin/TCF transcriptional activity inhibitor, N-cadherin or CD44 siRNAs. Taken together, our results suggest a new molecular mechanism of galectin-3-mediated cell adhesion in cancer metastasis.
研究领域:
肿瘤学
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细胞生物学
·
分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 18
·10887
·
IF 6.208
Jing Jie,Guomu Liu,Jingyue Feng,Desheng Huo,Yixuan Wu,Hongyan Yuan,Guixiang Tai,Weihua Ni
Our previous study found that CpG oligodeoxynucleotides 1826 (CpG 1826), combined with mucin 1 (MUC1)-maltose-binding protein (MBP) (M-M), had certain antitumor activity. However, this combination is less than ideal for tumor suppression (tumors vary in size and vary widely among individuals), with a drawback being that CpG 1826 is unstable. To solve these problems, here, we evaluate MF59/CpG 1826 as a compound adjuvant with M-M vaccine on immune response, tumor suppression and survival. The results showed that MF59 could promote the CpG 1826/M-M vaccine-induced tumor growth inhibition and a Th1-prone cellular immune response, as well as reduce the individual differences of tumor growth and prolonged prophylactic and therapeutic mouse survival. Further research showed that MF59 promotes the maturation of DCs stimulated by CpG1826/M-M, resulting in Th1 polarization. The possible mechanism is speculated to be that MF59 could significantly prolong the retention time of CpG 1826, or the combination of CpG 1826 and M-M, as well as downregulate IL-6/STAT3 involved in MF59 combined CpG 1826-induced dendritic cell maturation. This study clarifies the utility of MF59/CpG 1826 as a vaccine compound adjuvant, laying the theoretical basis for the development of a novel M-M vaccine.Keywords:MF59;CpG 1826;compound adjuvant;tumor vaccine;MUC1-MBP;dendritic cells
研究领域:
免疫学
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肿瘤学
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疫苗学
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细胞生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Changcai Teng,Wenjing Ma,Jinfeng Liu,Juan Hou,Yalong Zhang,Xiongyan Meng,Yannan Xue,Zhen Wang,Jiajia Wang,Dexiang Chen,Qiang Sui,Qi Gao,Xia Li,Tiehai Li,Chengli Zong
Aberrantly glycosylated Mucin 1 (MUC1) is frequently over-expressed in epithelial cancers, making it an attractive target for cancer vaccines. Over the past two decades, multiple MUC1-based vaccines have been investigated clinically, yet they have generally shown limited efficacy due to challenges such as low immunogenicity, difficulty in overcoming immune tolerance, and potential issues related to glycosylation effects and antigen presentations. To advance the understanding of MUC1 vaccines, we report an efficient chemo-enzymatic approach for the preparation of four MUC1 antigen variants with different glycoforms through liquid-phase glycopeptide synthesis. These antigen were conjugated with CRM197 to generate various glycopeptide-protein conjugate vaccines, and their immunogenicity was evaluated based on total and subtype antibody titers, binding affinity, complement-dependent cytotoxicity (CDC) activity, and antibody-dependent cellular phagocytosis (ADCP). The combination of MPL and QS21 adjuvants with STn-MUC1-CRM197 conjugate induced a potent Th1-biased immune response, evidenced by elevating IgG2a titers and stronger antibody binding to MCF-7 cells. This formulation demonstrated superior CDC activity, ADCP activity and binding affinity to human breast cancer tissues in immuno-histochemical assays. The synergistic effect of specific adjuvants and glycosylated MUC1 conjugates offers a strategic avenue for MUC1 cancer vaccine development.
研究领域:
癌症研究
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免疫学
·
化学
·
药学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Rong Hu,Wei Wen,Qingling Wang,Huayu Xiong,Xiuhua Zhang,Haoshuang Gu,Shengfu Wang
A novel platform based on a hairpin oligonucleotide (HO) switch, gold nanoparticles (AuNPs), and enzyme signal amplification for the ultrasensitive detection of mucin 1 protein (MUC1) was developed in this assay. This HO aptamers and horseradish peroxidase (HRP) were immobilised on the AuNPs to yield HO–AuNP–HRP conjugates. AuNPs were used as labels and bridges between the HO and HRP. HRP was also used as label for catalysing the oxidation of o-phenylenediamine by H 2 O 2 . The reaction product was 2,3-diaminophenazine (DAP), which was reduced and could be detected at surface of modified electrode . The reduction signal of DAP was used as a probe for the sensitive detection . After the recognition between oligonucleotide and MUC1, biotin was exposed. Biotin, along with the conjugate , was captured by streptavidin onto the surface of modified electrode. Therefore, the detection of target MUC1 which was a membrane-associated glycoprotein of the mucin family could be sensitively transduced via detection of the electrochemical reduction signal of DAP. Compared to other aptasensors , this biosensor has a good linear correlation ranges from 8.8 nM to 353.3 nM and a lower detection limit of 2.2 nM for MUC1. The proposed method provided a new electrochemical approach for the detection of MUC1.
研究领域:
生物传感器
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分析化学
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癌症诊断
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纳米技术
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生物化学
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DOI
PubMed
高影响因子
ACS Pharmacology & Translational Science
·
2024
·
IF 4.9
Jing-Jing Du,Shi-Hao Zhou,Jin Liu,Xing-Yuan Zhong,Ru-Yan Zhang,Wen-Xiang Zhao,Yu Wen,Zhen-Hong Su,Zheng Lu,Jun Guo
The tumor-associated antigen MUC1 is an attractive target for immunotherapy, however, its weak immunogenicity limits the induction of antitumor immune responses. To overcome this limitation, in this study, MUC1 glycopeptide was covalently linked with a diphtheria toxin-derived T-helper epitope (DT331–345). Subsequently, the resulting DT-MUC1 glycopeptide was physically mixed with natural killer T cell agonist αGalCer to explore their immunomodulatory synergy. Biological results demonstrated that compared to MUC1+αGalCer and DT-MUC1 groups, the specific IgG antibody titer of DT-MUC1+αGalCer group increased by 189- and 3-fold, respectively, indicating that the diphtheria toxin-derived T-helper epitope synergistically enhanced MUC1 immunogenicity with αGalCer. Moreover, the DT-MUC1+αGalCer vaccine induced potent cellular immune responses and significantly inhibited the growth of B16-MUC1 tumors in vivo. Furthermore, it was found that the anti-MUC1 IgG antibody titer induced by DT-MUC1+αGalCer was equivalent to that induced by palmitoylated DT-MUC1+αGalCer (P1-DT-MUC1+αGalCer) and significantly higher than that induced by doubly palmitoylated DT-MUC1+αGalCer (P2-DT-MUC1+αGalCer), suggesting that the easily synthesized DT-MUC1 may not require lipid chain modification and already possess good amphiphilicity. This is the first time that a diphtheria toxin-derived helper T-helper epitope was covalently linked to a glycopeptide antigen to enhance its immunogenicity, and this study may provide an effective vaccine design strategy for MUC1-targeted antitumor vaccines and offer novel insights into the design of fully synthetic peptide vaccines.
研究领域:
免疫学
·
肿瘤学
·
疫苗开发
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Haiqian Xia,Lingzi Xiong,Ruoyu Huang,Ning Liu,Muhammad Muhammad,Jingfang Hong,Qing Huang
Breast cancer remains a global health challenge, with an increasing number of cases necessitating innovative approaches to streamline patient management prior to treatment. In this study, we present a comprehensive aptamer-involved surface-enhanced Raman spectroscopy (aptamer-SERS)-based protocol specifically designed for large-scale clinical screening of the circulating protein MUC1 overexpressed in the majority of breast cancer cases. Central to our approach was a “sandwich” assay, where MUC1 was anchored between aptamer-functionalized bimetallic core-shell nanoparticles (NPs) and magnetic nanobeads. The biosensor was applied to monitor MUC1 in serum samples through SERS signals from the reporter molecule 4-ATP, exhibiting a strong linear correlation across a wide dynamic range and the lower the limit of detection (LOD) of 2.96 fg/mL. To support clinical decision-making, the protocol was integrated with a machine learning (ML)model for the classification of SERS signal patterns, demonstrating performance metrics of 96% accuracy and 93.7% specificity when applied to diverse serum samples. This integration enabled robust, non-invasive preclinical screening that informs therapeutic regulation and patient monitoring long before clinical symptoms present. By establishing a scalable framework for continuous monitoring of MUC1 levels across large populations, our study offered a forward-thinking tool, namely, dual-aptamer-SERS biosensor, that may optimize individualized treatment planning and improve overall clinical management strategies.
研究领域:
生物医学工程
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肿瘤学
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分析化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-11
·
IF 5.3
Zhan Ying,Mao Yichun,Sun Pei,Liu Chenbin,Gou Hongquan,Qi Haipeng,Chen Guifang,Hu Song,Tian Bo
Abstract Tumor-associated antigen (TAA)–based diagnosis has gained prominence for early tumor screening, treatment monitoring, prognostic assessment, and minimal residual disease detection. However, limitations such as low sensitivity and difficulty in extracting non-specific binding membrane proteins still exist in traditional detection methods. Upconversion luminescence (UCL) exhibits unique physical and chemical properties under wavelength near-infrared light excitation. Rolling circle amplification (RCA) is an efficient DNA amplification technique with amplification factors as high as 10 5 . Therefore, the above two excellent techniques can be employed for highly accurate imaging analysis of tumor cells. Herein, we developed a novel nanoplatform for TAA-specific cell imaging based on UCL and RCA technology. An aptamer–primer complex selectively binds to Mucin 1 (MUC1), one of TAA on cell surface, to trigger RCA reaction, generating a large number of repetitive sequences. These sequences provide lots of binding sites for complementary signal probes, producing UCL from lanthanide-doped upconversion nanoparticles (UCNPs) after releasing quencher group. The experimental results demonstrate the specific attachment of upconversion nanomaterials to cancer cells which express a high level of MUC1, indicating the potential of UCNPs and RCA in tumor imaging. Graphical Abstract
研究领域:
生物医学工程
·
分子诊断学
·
纳米医学
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DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 86
·54638-54643
·
IF 3.108
Yuhong Liu,Jinzha Zhang,Yang Shen,Jinduo Yan,Zaiying Hou,Chun Mao,Wenbo Zhao
Transition metal ions, such as those generated through MoS2 material, possess an intrinsic fluorescence quenching property towards organic dye molecules; thus, they can be used to construct biosensors as quenchers. However, we found that the conventional bulk MoS2 blocks the view of fluorescence imaging, and is incapable of tracing and visualizing mucin 1-overexpression cancer cells. Herein, a FAM fluorophore-labeled ssDNA fluorescent probe (P0-FAM) stacked on the surface of MoS2 quantum dots (QDs) was used to construct a MoS2 QDs–P0-FAM biosensor. MoS2 QDs exhibit a high fluorescence quenching ability towards fluorescent dyes, possess large specific surface area and a large number of active sites to adsorb and quench more fluorescent probes, promoting sensitivity between quenching and the recovery signal. In addition, the lighter color of unstack-MoS2 QDs is beneficial to define the location of cancer cells compared to MoS2 nanosheets. The novel MoS2 QDs-based biosensor demonstrates high sensitivity to MUC1 with a detection limit of 0.5 nM, and may become an important tool toward the detection of cancer cells.
研究领域:
生物化学
·
纳米医学
·
分子诊断
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产品关联
DOI
高影响因子
Cancer Nanotechnology
·
2023
·Vol. 14
·期 1
·1-16
·
IF 5.7
Bai Zhiqiang,Zhao Lu,Feng Haidi,Xin Zhihui,Wang Chaoyu,Liu Zhixiong,Tian Maozhong,Zhang Haifei,Bai Yunfeng,Feng Feng
Background Ti 3 C 2 is a type of transition metal carbides and nitrides (MXenes) with high light-to-heat conversion efficiency property, which has been widely used in cancer treatment recently. In fact, active targeting delivery of MXenes nanomaterials with targeting molecule could enhance the therapeutic efficacy. However, targeted therapy of MXenes has not been further studied in the past. Aptamers (Apt) with excellent affinity and high specificity properties have been widely used as targeting tools. Predictably, the incorporation of Apt into Ti 3 C 2 nanomaterials will offer an unprecedented opportunity in the research fields of cancer targeted therapy. Results Transmembrane glycoprotein mucin 1 (MUC1) is overexpressed on the surface of MCF-7 cells, and MUC1 Apt (Apt-M) could target MCF-7 cells with high affinity and specificity. Here, a smart targeting nanotherapeutic system Ti 3 C 2 /Apt-M was fabricated, which could specifically recognize and enter in MCF-7 cells. Benefitting from the desirable targeted performance of Apt-M, MCF-7 cells completed the ingestion process of Ti 3 C 2 /Apt-Mf nanosheets within 4 h, and Apt-M facilitated the entry of the Ti 3 C 2 /Apt-Mf nanosheets into MCF-7 cells. Besides, Ti 3 C 2 /Apt-M nanosheets exhibited the potential as an outstanding photothermal agent (PTA) because of the photothermal performance inherited from wrapped Ti 3 C 2 nanosheets. As demonstrated, upon 808 nm laser irradiation, the Ti 3 C 2 /Apt-M nanotherapeutic system displayed a satisfactory antitumor effect by targeted photothermal therapy both in vitro and in vivo. Conclusion This study provides a new idea for the development of MXenes nanotherapeutic system with high active targeting performance. Graphical Abstract
研究领域:
纳米技术
·
癌症治疗
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材料科学
·
生物医学工程
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Yang Zhou,Xin Gu,Hongmin Gao,Hehua Zhang,Ying Liu,Dong Chang,Hongzhi Pan
The abnormally high expression of MUC1 in various malignant tumors makes it a crucial biomarker for early cancer diagnosis. This study synthesized a Cu-MOF@PtPdNPs nanocomposite exhibiting robust peroxidase-like activity via an in-situ reduction method. By integrating this nanomaterial with a dual-aptamer recognition sandwich strategy, an efficient colorimetric sensor was constructed for MUC1 detection. The nanozyme modified with aptamer2 (Cu-MOF@PtPdNPs-Apt2) was used to specifically recognize the target MUC1. Then, magnetic beads modified with aptamer1 (MB-Apt1) were used to enrich and separate the Cu-MOF@PtPdNPs-Apt2@MUC1, forming sandwich complexes. The sandwich complexes were separated and removed using magnets, and the remaining Cu-MOF@PtPdNPs-Apt2 in the supernatant was used for colorimetric detection. This sensor exhibits a linear range from 1 ng/mL to 100 μg/mL, alongside a limit of detection (LOD) of 0.211 ng/mL. Benefiting from the ingenious sandwich architecture, the sensor demonstrates high selectivity toward MUC1 and shows a good response in human serum, with recovery rates between 92.00% and 97.82%. In conclusion, this study provides a fast and sensitive technical platform for MUC1 detection.
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DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Kaida Kuang,Yao Lu,Yang Chen,Pei Zhang,Nengqin Jia
The traditional luminol electrochemiluminescence (ECL) sensing suffers from low signal response and instability issues. Here, an Au/ZnCuS double-enhanced g -C 3 N 4 -supported luminol ECL aptasensor is constructed for the sensitive detection of human mucin 1 (MUC1). In this platform, g -C 3 N 4 of a large specific surface area is beneficial to load more luminol illuminants. Au nanoparticles promote the decomposition of H 2 O 2 coreactants to generate more reactive oxygen (•OH and O 2 • − ) intermediates, while ZnCuS can immobilize the aptamer and simultaneously catalyze H 2 O 2 decomposition, realizing the double-wing signal amplification. Under optimal conditions, this sensor shows a good detection capability within 1.0 × 10 −4 –1.0 × 10 3 ng mL −1 and a low detection limit of 5.0 × 10 −5 ng mL −1 , as well as ideal stability, selectivity, and reproducibility. This double-enhanced aptasensor highlights a new signal-enhancement approach for early biomarker detection.
研究领域:
分析化学
·
生物医学工程
·
传感技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）