INSTRUMENTATION SCIENCE & TECHNOLOGY
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2026
·
IF 1
Tianci Xu,Yan Jiang,Fangran Jia,Runze Zhang,Boyi Shao,Qianwen Li,Chengze Li,Han Gao,Hongrui Han,Chenchen Zhang,Yongjie Yang
Leuco-malachite green (LMG), a metabolite of malachite green (MG), exhibits stronger bioaccumulation and higher toxicity to humans. Therefore, development of a rapid and sensitive detection method for LMG is of great significance. In this study, LMG-specific split aptamers (SPALMG) with a dissociation constant (Kd) of 1.538 ± 0.1261 μM and enhanced affinity compared to the parent aptamer are generated using A5b-12C, a high-affinity variant, as the parent aptamer for LMG detection. Subsequently, a colorimetric aptasensor is developed, employing thiol-modified SPAlmg as probes and gold nanoparticles (AuNPs) as signal indicators. In the presence of LMG, the SPAlmg preferentially bind to LMG to form a ternary complex, causing the AuNPs to aggregate and induce a color change from red to blue. The sensor exhibits high sensitivity and specificity, with a limit of detection (LOD) of 46 nM, a linear range of 1–7 μM, and good recoveries of 96–113%. Compared with other studies, a more excellent LOD is achieved. This work provides a novel affinity probe that holds great promise for the development of sandwich-type sensors for LMG detection.
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高影响因子
INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
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2013
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IF 1.233
Zhengzhong Lin,Quanshu Wu,Hongyuan Zhang,Yidong Lin,Zhiyong Huang
Molecularly imprinted polymer (MIP) microspheres were synthesized through precipitation polymerization using malachite green (MG) as template, methacrylic acid (MAA) as monomer, and trimethylolpropane trimethacrylate (TRIM) as cross-linker. The microsphere structure of MIP was characterized by IR spectroscopy and SEM. The influence of preparation conditions such as monomer and cross-linker dosages on the polymer absorption of MG in acetonitrile solution was also explored. Under the optimum synthesis conditions (0.25 mmol MG, 1.5 mmol MAA, 2.5 mmol TRIM, 40 mL acetonitrile), the prepared MIP microspheres have a binding capacity as high as 2000 µg g−1 of MG with an imprinting factor of above 4.0. The result suggests that the prepared MIP microspheres are promising material for the selective extraction of MG in complicated matrix solutions.
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高影响因子
RSC Advances
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2021
·Vol. 11
·期 26
·16090-16095
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IF 3.361
Heng-Fang Xiang,Jia-Kun Xu,Jiao Liu,Xin-Zhi Yang,Shu-Qin Gao,Ge-Bo Wen,Ying-Wu Lin
Synthetic dyes such as malachite green (MG) have a wide range of applications. Meanwhile, they bring great challenges for environmental security and cause potential damages to human health. Compared with traditional approaches, enzymatic catalysis is an emerging technique for wastewater treatment. As alternatives to natural enzymes, artificial enzymes have received much attention for potential applications. In previous studies, we have rationally designed artificial enzymes based on myoglobin (Mb), such as by introducing a distal histidine (F43H mutation) and creating a channel to the heme pocket (H64A mutation). We herein show that the artificial enzyme of F43H/H64A Mb can be successfully applied for efficient biodegradation of MG under weak acid conditions. The degradation efficiency is much higher than those of natural enzymes, such as dye-decolorizing peroxidase and laccase (13–18-fold). The interaction of MG and F43H/H64A Mb was investigated by using both experimental and molecular docking studies, and the biodegradation products of MG were also revealed by UPLC-ESI-MS analysis. Based on these results, we proposed a plausible biodegradation mechanism of MG. With the high-yield of overexpression in E. coli cells, this study suggests that the artificial enzyme has potential applications in the biodegradation of MG in fisheries and textile industries.
研究领域:
环境科学
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生物化学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Wenying Gui,Huan Wang,Yang Liu,Qiang Ma
A dual-emission molecularly imprinted mesoporous microspheres was designed for specific recognition and highly sensitive and selective determination of malachite green (MG). In this ratiometric fluorescent sensor, green fluorescent CdTe quantum dots (QDs)-doped into the core of silica mesoporous microspheres serves as a reference and provides a built-in correction for environmental effects. The other red fluorescent CdTe QDs are embedded around the pores of the silica microspheres as analytical tag. Then, molecular imprinting technique are integrated with the ratiometric sensor to provide high selectivity for MG . When the photoluminescence (PL) of red CdTe QDs is selectively quenched in the presence of MG, a visual color change from orange to green can be observed by naked eyes. The sensing method has a linear response in the range of 27.4 nM–137 μM with a detection limit of 17.0 nM. Moreover, there is an excellent recognition specificity for MG over other water pollutants. The applicability of this method is evaluated through the determination of MG in river water and lake water samples with satisfactory results.
研究领域:
分析化学
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环境科学
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高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2018
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IF 4.005
Yi Hu,Yulian Li,Junyong He,Tao Liu,Kaisheng Zhang,Xingjiu Huang,Lingtao Kong,Jinhuai Liu
Industrial waste, urban sewage and aquaculture have led to severely increased grades of environment pollutants such as dyes, pesticides and fertilizer. The use of technologies for purifying contaminated waters can be difficult and toxic due to the anti-photolysis, anti-oxidation and anti-bio-oxidation characteristics of organic pollutants, and there is therefore a significant need for new approaches. Here, we report methods of Fenton oxidation and EDTA-Fe(III) Fenton-like oxidation which can be used to degrade malachite green (MG: a dye and antibiotic-like substance) from contaminated water. Compared with the degradation rate (59.34%) of the Fe(III)/H 2 O 2 Fenton process, the EDTA-Fe(III) Fenton-like oxidation got a better degradation rate (92.7%) at neutral pH conditions . By conducting a series of parallel controlled experiments (changing parameters such as the reactant concentration , temperature, and pH), we report the relationships between the degradation effect and different parameters, and we fitted their pseudo first order kinetic curves. Furthermore, we repeated to adjustment of the concentrations of MG in solutions to test the cycle performance and catalytic activities of EDTA-Fe(III)/H 2 O 2 system and it showed good repeatability in the first five rounds and all of them keep the degradation efficiencies greater than 80%. By conducting comparative spin-trapping electron paramagnetic resonance (EPR) experiments, we showed indirectly that the OH contributes to the degradation of MG. Additionally, the results of the EPR experiments showed that EDTA contributes to the generation of OH in the EDTA-Fe(III)/H 2 O 2 Fenton-like system. By conducting total organic carbon (TOC) analysis experiments, we found that EDTA was also oxidized to some extent during the degradation of MG. In all, the findings of this work widen the range of the optimal pH values up to neutral condition for degradation of MG by use of EDTA-Fe(III) Fenton-like system. And this system could be used as one approach for the degradation of organic pollutants at neutral conditions and provide some initial information regarding EDTA-Fe(III) Fenton-like oxidations. It's significant for the expansion of the homogenous Fenton-like family and its application in the field of water treatment .
研究领域:
环境科学
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化学工程
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水处理技术
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高影响因子
RSC Advances
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2014
·Vol. 4
·期 105
·60987-60994
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IF 3.708
Hongzhi Wang,Yu Wang,Su Liu,Jinghua Yu,Wei Xu,Yuna Guo,Jiadong Huang
An RNA aptamer-based electrochemical biosensing strategy has been developed for sensitive and selective detection of malachite green (MG). This biosensor is fabricated by the self-assembly of a thiolated MG aptamer (MGA) on AuNPs/graphene–chitosan nanocomposite modified glass carbon electrode. In addition, a short alkanethiol is further assembled on the AuNPs surface to generate uniform packing and reduce nonspecific adsorption. When the modified electrode is incubated in the presence of MG, MGA combines specifically with MG, which causes the horseradish peroxidase (HRP)-labelled MG antibody close to the electrode surfaces. As a result, MG detection is realized by outputting a redox current from electro-reduction of the hydrogen peroxide reaction catalyzed by HRP. Differential pulse voltammetry (DPV) is performed to record the signal responses. The results reveal the biosensor displays a very low detection limit as low as 16.3 pg mL−1 and a wide linear range from 1 × 10−4 to 10 μg mL−1 of MG. Hence, this proposed RNA aptamer-based electrochemical strategy may offer a simple, rapid, cost-effective, highly selective and sensitive method for the quantification of MG.
研究领域:
分析化学
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环境科学
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生物传感器
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纳米技术
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高影响因子
FOOD CHEMISTRY
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2017
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IF 4.529
Lu Li,Ai-hong Peng,Zheng-zhong Lin,Hui-ping Zhong,Xiao-mei Chen,Zhi-yong Huang
A highly selective and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of malachite green (MG) using a molecularly imprinted polymer (MIP) film as bionic antibody. The MIP film, based on the self-polymerization of dopamine, was fabricated on the surfaces of a 96-well microplate. It showed specific recognition for MG in aqueous solution. A direct competitive ELISA method was established with the sensitivity reaching 10.31 μg L −1 and the detection limit being 0.3 μg L −1 . The cross-reactivity of two structural analogues to MG was less than 10%. The average recovery tested by MG standard spiking was 88.8% for bass and 90.4% for water, and the relative standard deviations were less than 3.6%. All the above results indicated that the developed method could be used to detect MG in fish and water samples rapidly, specifically and accurately.
研究领域:
分析化学
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环境科学
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食品科学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Yufei Wang,Yiyang Lin,Wei Tao,Yuqing Zhu,Jiacheng Niu,Yikun Liao,He Feng
Malachite green (MG) is a triphenylmethane dye resistant to degradation, possessing high toxicity, carcinogenicity, and mutagenicity, and co-existing bacterial contamination affects water safety. In this work, CuO-loaded sepiolite (CuO/Sep) nanocomposites were synthesized by an ammonia-driven deposition–precipitation method (ADP) to simultaneously remove MG and exhibit antibacterial activity. The nanocomposite with 10 wt% CuO had a specific surface area of 166.5 m 2 /g. The adsorption kinetics conformed to the pseudo-second-order kinetic model, and the equilibrium adsorption data could be described by the Langmuir model. At 295 K, the maximum monolayer adsorption capacity reached 809.6 mg/g, and the decolorization rate for an MG solution with an initial concentration of 50 mg/L reached 99%. The adsorption process involved a synergistic effect of physical and chemical adsorption, representing an endothermic reaction (ΔH = +55.36 kJ/mol). In addition, the composite exhibited good antibacterial activity, with antibacterial rates reaching 99.9% against Staphylococcus aureus and 97.5% against Escherichia coli . These results indicate that the synthesized CuO/Sep nanocomposite possesses both good MG adsorption capacity and broad-spectrum antimicrobial properties. Download: Download high-res image (277KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Shan Huang,Danyu Lv,Yuhuan Geng,Fuhao Yue,Xinan Zeng,Mengwai Woo,Zhong Han,Ji Ma
Malachite green (MG) is a toxic contaminant commonly found in aquaculture, making its effective removal and detection crucially important. In this study, MIL-100(Fe) was synthesized via a microwave-assisted hydrothermal method under optimized conditions (150 °C, 200 W, 30 min; FeCl₃·6H 2 O:H₃BTC = 2:3). The resulting material exhibited excellent structural stability, a high specific surface area (1169.9 m 2 /g), and a remarkable MG adsorption capacity of 714.29 mg/g. To enable practical application and reusability, MIL-100(Fe) was immobilized onto a melamine sponge (MeS) using polyvinylidene fluoride (PVDF) as binding agent, forming a recyclable MeS/PVDF/MOF composite. When applied for solid-phase extraction of MG in conjunction with UV detection, the composite demonstrated high recovery rates (92.1–99.4 %), a low detection limit (2.29 μg/L), and consistent performance with over 80 % recovery after six reuse cycles in both real fish and wastewater samples. This study presents a simple, efficient and sustainable strategy for the adsorption and detection of MG in aquatic food matrices.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-10
·
IF 5.3
Chen Zanlin,Li Haiming,Chen Yian,Han Shuangyan
A high-affinity aptamer was developed using a molecular docking approach, leading to the establishment of an electrochemical aptamer biosensor for the detection of malachite green (MG). The aptamer designated as MG-15 was derived from a rational truncation of MG-36, yielding a dissociation constant (K d ) of 45.4 \(\pm\) 17.6 μM. Further enhancements were achieved by completely mutating the binding site of MG-15, resulting in a higher affinity aptamer (MG-15-GGT) with a lower K d of 2.71 \(\pm\) 1.95 μM. This sensor exhibited a limit of detection (LOD) value of 2.74 pM and demonstrated excellent specificity, with recoveries ranging from 87.1 to 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety. Graphical
研究领域:
分析化学
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食品安全
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分子识别
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生物传感器
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环境监测
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药物残留检测
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分子生物学
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高影响因子
CHEMICAL PAPERS
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2024
·
IF 2.1
Guo Jinxiu,Kong Tao,Zhang Huaijie,Ma Runtian,Ma Zijun,Hu Bing
The development of rapid and sensitive malachite green (MG) detection methods is particularly important for food safety supervision and ecological protection. Using fulvic acid (FA) as a stabilizer and NaBH 4 as a reducing agent, ellipsoidal gold nanoparticles (AuNPs) with the average particle size of 5–20 nm were successfully prepared. Based on the interaction between fulvic acid protected gold nanoparticles (FA-AuNPs) and MG, the color change of FA-AuNPs from ruby red to blue was observed, the corresponding absorbance ratio change of gold nanoparticles at 611 nm and 529 nm (A611/A529) was also studied. Through the optimization of experimental conditions, the linear relationship between A611/A529 with the concentration of MG was established. The detection limit and linear range of this method were 0.02 μM and 0.04–2 μM, respectively. This method was successfully applied to detect malachite green in three different water samples with the recovery of 97.4–110.8%. Graphical abstract
研究领域:
分析化学
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环境科学
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食品安全
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高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 5
·1246-1254
·
IF 2.413
Zhou Yanli,Li Xiaoqiao,Pan Zhonghao,Ye Baoxian,Xu Maotian
Cu-based metal-organic frameworks modified by silver (Ag/Cu-MOFs) were in situ fabricated through a one-step direct synthesis. The as-prepared mixed-node MOFs were modified on glassy carbon (GC) electrode, which was utilized as a voltammetric sensor for probing malachite green. Compared with the bare GC electrode, the introduced Ag/Cu-MOF film on the electrode surface dramatically improved the sensitivity of the sensor response according to the accumulation and catalytic activity of Ag/Cu-MOFs for malachite green. The developed electrochemical sensor exhibited a low detection limit of 2.2 nM with a wide linear range of 10–140 nM and high anti-interference ability. The feasibility of the assay was verified by test of malachite green in fish sample. The proposed strategy presents valuable information for the evaluation of the food safety in aquaculture industry.
研究领域:
分析化学
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环境科学
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食品科学
·
材料科学
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高影响因子
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