ACS Applied Materials & Interfaces
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2020
·
IF 8.758
Simin Peng,Zhen Luo,Shaojie Wang,Jiajie Liang,Chao Yuan,Zhikang Yuan,Jun Hu,Jinliang He,Qi Li
Heterogeneous dielectric materials such as dielectric polymer nanocomposites have attracted extensive attention because of their exceptional insulating and dielectric performance, which originates from the unique space charge dynamics associated with the various interfacial regions. However, the space charge distribution and transport in polymer nanocomposites remain elusive due to the lack of analytical methods that can precisely probe the charge profile at the nanoscale resolution. Although a few studies have explored the possibility of using scanning probe techniques for characterizing the local charge distribution, the interference from induced electrical polarization of the material has been unfortunately ignored, leading to inaccurate results. In this contribution, we report an open-loop Kelvin probe force microscopy (KPFM) method with nanoscale resolution for the direct detection of the space charge profile in dielectric polymer nanocomposites. Unlike the conventional studies where a vertical direct current (DC) voltage is applied on the sample through the probe to evoke the charge injection and transport in dielectric polymer nanocomposites, the present method is established based on a delicate electrode configuration where a lateral electric field is allowed to be applied on the sample during the KPFM test. This special testing configuration enables real-time charge injection and transport without inducing the electrical polarization of material along the vertical direction, which gives rise to clean mapping of space charges and reveals the interfacial charge trapping in polymer nanocomposites. This work provides a robust and reliable method for studying the sophisticated charge transport properties associated with the various interfacial regions in heterogeneous dielectric materials.
研究领域:
材料科学
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电气工程
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纳米技术
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DOI
PubMed
高影响因子
Nature Chemical Biology
·
2024
·
IF 12.9
Niu Hongyan,Li Meng-Yin,Gao Yan,Li Jun-Ge,Jiang Jie,Ying Yi-Lun,Long Yi-Tao
Sulfation is considered the most prevalent post-translational modification (PTM) on tyrosine; however, its importance is frequently undervalued due to difficulties in direct and unambiguous determination from phosphorylation. Here we present a sequence-independent strategy to directly map and quantify the tyrosine sulfation states in universal native peptides using an engineered protein nanopore. Molecular dynamics simulations and nanopore mutations reveal specific interactions between tyrosine sulfation and the engineered nanopore, dominating identification across diverse peptide sequences. We show a nanopore framework to discover tyrosine sulfation in unknown peptide fragments digested from a native protein and determine the sequence of the sulfated fragment based on current blockade enhancement induced by sulfation. Moreover, our method allows direct observation of peptide sulfation in ultra-low abundance, down to 1%, and distinguishes it from isobaric phosphorylation. This sequence-independent strategy suggests the potential of nanopore to explore specific PTMs in real-life samples and at the omics level.
研究领域:
生物化学
·
分子生物学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Peifeng Yu,Jie Zhan,Xiaobing Zhang,Kangwang Wang,Lingyong Zeng,Kuan Li,Chao Zhang,Longfu Li,Ying Liang,Kai Yan,Yan Sun,Huixia Luo
Exploring high-efficiency and low-cost electrocatalysts is valuable for water-splitting technologies. Recently, Si-group compounds have attracted increasing attention in electrocatalysis, considering the abundant Si-group elements on Earth. However, Si-group compounds for HER electrocatalysis have not been systematically studied. In this study, we unveil the activity trends of non-noble metal catalyst A15-type V 3 M ( i.e. , V 3 Si, V 3 Ge, and V 3 Sn) superconductors and show that V 3 Si is the most efficient HER catalyst because of the high electronic conductivity and suitable d -band center. Among them, the V 3 Si only requires 33.4 mV to reach 10 mA cm −2 , and only 57.6 mV and 114.6 mV are required to attain a high current density of 100 mA cm −2 and 500 mA cm −2 , respectively. These low overpotentials are close to the 34.3 mV at 10 mA cm −2 of state-of-art Pt/C (20 %) but superior to 168.5 mV of Pt/C (20 %) at 100 mA cm −2 . Furthermore, the V 3 Si illustrates exceptional durability with no obvious decay in the 120 h at the different current densities ( i.e. , 10–250 mA cm −2 ). The excellent HER activity of V 3 Si alloy can be ascribed to the synergies of superior electronic conductivity and suitable d -band center. Moreover, DFT calculations reveal that |ΔG H* | is decreased after introducing the V to Si. Beyond offering a stable and high-performance electrocatalyst in an acidic medium, this work inspires the rational design of desirable silicide electrocatalysts .
研究领域:
材料科学
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电催化
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能源技术
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2025
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IF 8.2
Kai Wang,Lifeng Wang,Jiawei Si,Rui Wang,Ziyuan Wang,Chuyuan Gao,Jin Yang,Xiaohan Yang,Hanqiang Zhang,Lei Han
As one of the core parts of the Internet-of-things (IOTs), multimodal sensors have exhibited great advantages in fields such as human–machine interaction, electronic skin, and environmental monitoring. However, current multimodal sensors substantially introduce a bloated equipment architecture and a complicated decoupling mechanism. In this work we propose a multimodal fusion sensing platform based on a power-dependent piecewise linear decoupling mechanism, allowing four parameters to be perceived and decoded from the passive wireless single component, which greatly broadens the configurable freedom of a sensor in the IOT. A systematic model is employed to analyze the linear sensing properties and ensure the feasibility of the scheme. The excitation power dependence provides an efficient and quantitative linear decoupling strategy of unidentified combinations for multiple stimuli. As a validation for a wearable device such as electronic skin (e-skin), the functionalized sensing film polyaniline/graphene oxide (PANI/GO) is served to synchronously monitor humidity, temperature, ultraviolet, and proximity through the mapping in resonant frequency (fs). Compared with the output errors of ∼18.00%, ∼17.50%, ∼15.00%, and ∼20.00%, the maximum experimental errors of temperature, humidity, ultraviolet, and proximity are 5.70%, 4.00%, 5.00%, and 8.30% after decoupling, respectively. In general, the developed single-component multimodal fusion sensing platform offers a strategic advantage for a miniaturization, passive wireless, and inexpensive (less than $1) signal identification system with a facile circuit layout.
研究领域:
物联网
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材料科学
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传感器技术
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产品关联
DOI
PubMed
高影响因子
Neuroscience Bulletin
·
2026
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IF 6.8
Feng Wei,Pang Lijuan,Liu Chun-Jie,He Jiuming,Wang Lisi,Xu Zhenyu,Zeng Yinglong,Yuan Zengqiang,Zhang Chao
The brain supports consciousness through regional heterogeneity. However, the spatial complexity of its metabolome under anesthesia remains unclear. In this study, we established a spatial metabolomic atlas of the mouse brain under wakefulness, inhalation anesthesia, and injectable anesthesia. In total, 727 metabolites were identified and spatially mapped across 11 representative brain regions. We found that anesthesia significantly altered the brain metabolic landscape in a spatially dependent manner, particularly affecting lipid metabolism, amino acid pathways, and neurotransmitter-related metabolites. Furthermore, we monitored the cortical microvascular blood flow and blood oxygen levels during anesthesia. We observed that different anesthesia modalities induced distinct changes in cortical arteriovenous microvascular hemodynamics, suggesting a potential association between metabolic alterations and cerebrovascular regulation. We developed an open-access online database, the mouse brain spatial metabolome atlas ( https://mbsma.liulab.cloud/#/dashboard ), to facilitate access to and analysis of the dataset. Our study reveals anesthesia-induced spatial metabolic reprogramming in the brain, providing insights into neural metabolism and cerebrovascular dynamics, with implications for perioperative brain protection and safer anesthesia strategies.
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DOI
PubMed
高影响因子
Advanced Science
·
2020
·Vol. 7
·期 8
·1903185
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IF 15.84
Jingtan Zhu,Tingting Yu,Yusha Li,Jianyi Xu,Yisong Qi,Yingtao Yao,Yilin Ma,Peng Wan,Zhilong Chen,Xiangning Li,Hui Gong,Qingming Luo,Dan Zhu
Tissue optical clearing techniques have provided important tools for large-volume imaging. Aqueous-based clearing methods are known for good fluorescence preservation and scalable size maintenance, but are limited by long incubation time, insufficient clearing performance, or requirements for specialized devices. Additionally, few clearing methods are compatible with widely used lipophilic dyes while maintaining high clearing performance. Here, to address these issues, m-xylylenediamine (MXDA) is firstly introduced into tissue clearing and used to develop a rapid, highly efficient aqueous clearing method with robust lipophilic dyes compatibility, termed MXDA-based Aqueous Clearing System (MACS). MACS can render whole adult brains highly transparent within 2.5 days and is also applicable for other intact organs. Meanwhile, MACS possesses ideal compatibility with multiple probes, especially for lipophilic dyes. MACS achieves 3D imaging of the intact neural structures labeled by various techniques. Combining MACS with DiI labeling, MACS allows reconstruction of the detailed vascular structures of various organs and generates 3D pathology of glomeruli tufts in healthy and diabetic kidneys. Therefore, MACS provides a useful method for 3D mapping of intact tissues and is expected to facilitate morphological, physiological, and pathological studies of various organs.
研究领域:
生物医学工程
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神经科学
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病理学
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成像技术
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2025
·
IF 11.3
Yuyi Gu,Xiang Qi,Pengfei Huo,Ziwei Kou,Yijun Yin,Wentao Wang,Xia Huang,Peng Liang
Accurately characterizing the proton’s spatiotemporal distribution is critical for elucidating proton/electron generation and transfer mechanisms in electroactive biofilms (EABs). This study employed ratiometric fluorescence sensing for nondestructive, real-time pH mapping in current-producing EABs. The distribution of protons in EABs is determined by both their generation, which is electron donor-dependent, and their transfer, mediated by concentration gradients and buffering effects. Real-time pH mapping evidenced that under low organic conditions (≤0.4 g/L NaAc) in a PBS-free system, proton diffusion driven solely by concentration gradients prevented internal acidification (pH ≥ 6). However, elevated organics (0.8 g/L NaAc) triggered excessive proton accumulation exceeding transfer capacity, resulting in pronounced acidification (pH < 6) and subsequent electroactivity suppression. It also showed that while employing PBS maintained a neutral pH (∼7) for sustained current generation, it concurrently diminished the intrinsic proton concentration gradient, impairing proton diffusion efficiency. Finally, the electron flux was further derived in two ways: converted from the stoichiometric relationship with proton flux (Je, cal) and calculated from the measured current (Je, test) by applying proton flux, and a better linear fitting correlation was achieved when there was no PBS. This evidences the possibility to trace electron transfer through monitoring protons.
研究领域:
生物电化学
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微生物电化学
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环境生物技术
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荧光成像
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生物膜
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Wei Xu,Yanlan Zhu,Xiaolin Zhou,Haoyue Guo,Jingxin Wang,Ruiqi Zhu,Zhengwei Hu,Wei Ma,Xing Ma,Xiaojian Li,Xiaomin Xu
Organic electrochemical transistors (OECTs) emerge as promising neural electrodes owing to their superb biocompatibility, flexibility, on-site signal amplification capabilities, low operating voltage, and performance stability in aqueous conditions. Traditional implementations of OECT arrays have been limited by their relatively low channel count, large footprint, slow response time, and inadequate spatiotemporal resolution. Here, we report scalable high-density OECT arrays designed for high-reolution mapping of electrocorticogram signals. Our design features vertically stacked source and drain electrodes, achieving an array configuration with 1,024 channels at a density of 10,000 transistors per square centimeter and an average peak transconductance of 3.32 mS. For prototype demonstration, we apply a 64-channel array for the mapping of epileptic signals in mice, yielding a signal-to-noise ratio (SNR) of 32 dB, which significantly surpasses that of traditional platinum electrodes. The ultraflexible OECT array, featuring a total thickness of 4.2 µm, demonstrates high mechanical compliance, biocompatibility, and long-term stability, remaining effective for over three months post-implantation. This high-resolution, ultraflexible OECT array offers potential to precisely monitor neural activities across extensive brain areas, essential for both research and clinical applications.
研究领域:
神经科学
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生物医学工程
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材料科学
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产品关联
DOI
高影响因子
NUCLEIC ACIDS RESEARCH
·
2021
·Vol. 49
·期 7
·3672-3680
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IF 16.971
Wei Yue,Huang Qinqin,Tian Xihao,Zhang Mingmin,He Junkai,Chen Xingxiang,Chen Chao,Deng Zixin,Li Zhiqiang,Chen Shi,Wang Lianrong
DNA phosphorothioate (PT) modifications, with the nonbridging phosphate oxygen replaced by sulfur, governed by DndABCDE or SspABCD, are widely distributed in prokaryotes and have a highly unusual feature of occupying only a small portion of available consensus sequences in a genome. Despite the presence of plentiful non-PT-protected consensuses, DNA PT modification is still employed as a recognition tag by the restriction cognate, for example, DndFGH or SspE, to discriminate and destroy PT-lacking foreign DNA. This raises a fundamental question about how PT modifications are distributed along DNA molecules to keep the restriction components in check. Here, we present two single-molecule strategies that take advantage of the nucleophilicity of PT in combination with fluorescent markers for optical mapping of both single- and double-stranded PT modifications across individual DNA molecules. Surprisingly, PT profiles vary markedly from molecule to molecule, with different PT locations and spacing distances between PT pairs, even in the presence of DndFGH or SspE. The results revealed unprecedented PT modification features previously obscured by ensemble averaging, providing novel insights into the riddles regarding unusual target selection by PT modification and restriction components.
研究领域:
分子生物学
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遗传学
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生物化学
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DOI
PubMed
高影响因子
IEEE Access
·
2024
·
IF 3.4
Qinyao Luo,Yu Liu,Minyan Bi,Xi Kuai,Qin Tian,Yukai Sun,Sida Zhuang
Colorectal cancer is a major public health problem for its contribution to morbidity and mortality globally, and one of its major inducements is diet. Despite the achievements in the correlation studies about dietary habit and colorectal cancer, as an important component of dietary habit, taste preference has not received enough attention, its quantitative relationship with colorectal cancer has not been fully revealed. Using data from crowdsourced recipes, restaurant points of interest, and regional colorectal cancer mortality rates, this study quantitatively analysed the effects of different tastes on colorectal cancer, established a spatio-temporal link between taste and the disease, elucidating taste’s role in the spatial distribution of colorectal cancer. According to the analysis, Chinese taste preference presents significant spatial heterogeneity, and Chinese cuisines most favour ‘Spicy’ which makes up the top two risk factors of colorectal cancer with ‘Pungent’. Also, the interactions of Salty’ with other tastes might increase colorectal cancer risk, the same goes for ‘Oily’. The proposed quantitative analysis method based on crowdsourcing data can be applied to researches on other diseases, and the study can provide a new method and a new perspective for relevant studies.
研究领域:
肿瘤学
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营养流行病学
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地理空间分析
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公共卫生
·
饮食科学
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DOI
高影响因子
NANO LETTERS
·
2024
·
IF 9.6
Boyuan Shao,Shun Zhang,Yunfei Hu,Zetao Zheng,Hang Zhu,Liu Wang,Lingyu Zhao,Fang Xu,Luyang Wang,Mu Li,Jidong Shi
Mimicking the function of human skin is highly desired for electronic skins (e-skins) to perceive the tactile stimuli by both their intensity and spatial location. The common strategy using pixelated pressure sensor arrays and display panels greatly increases the device complexity and compromises the portability of e-skins. Herein, we tackled this challenge by developing a user-interactive iontronic skin that simultaneously achieves electrical pressure sensing and on-site, nonpixelated pressure mapping visualization. By merging the electrochromic and iontronic pressure sensing units into an integrated multilayer device, the interlayer charge transfer is regulated by applied pressure, which induces both color shifting and a capacitance change. The iontronic skin could visualize the trajectory of dynamic forces and reveal both the intensity and spatial information on various human activities. The integration of dual-mode pressure responsivity, together with the scalable fabrication and explicit signal output, makes the iontronic skin highly promising in biosignal monitoring and human–machine interaction.
研究领域:
材料科学
·
生物医学工程
·
电子学
·
人机交互
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DOI
高影响因子
PHARMACEUTICAL RESEARCH
·
2025
·Vol. 42
·期 1
·173-184
·
IF 4.3
Fang Jun-Ting,Wang Si-Tao,Wang Haibin,Fang Wei-Jie
Purposes In the peptide mapping reduction process for monoclonal antibodies (mAbs) and other proteins, the conventional reducing reagents β-mercaptoethanol (β-ME) and dithiothreitol (DTT) pose challenges due to their strong odor and toxicity at high concentrations. Cysteine (Cys), an essential amino acid for new protein synthesis, is an overlooked, nontoxic, and odorless reducing agent. This study presents a novel peptide mapping method using Cys as the reducing agent. Methods We explored the reducing effect of Cys at different concentrations and pH levels for peptide mapping analysis of a specific mAb (mAb-1), using DTT as a positive control. RP-HPLC analysis, including UV chromatogram comparison and overall similarity calculation, was conducted for comparison. LC–MS analysis was subsequently utilized to characterize the primary sequence of mAb-1. We also applied the method to other mAbs or proteins to demonstrate its wide applicability. Results The UV chromatogram and overall similarity of Cys as a reducing agent at concentrations ranging from 10 to 40 mM and pH levels between 7.0 and 11.0 were consistent with those of the positive control. Reduced concentrations of Cys or lower pH levels compromised reducing efficacy. This novel reducing method proficiently characterized the primary sequence of mAb-1, achieving an overall sequence coverage of 97%. In the analysis of other mAbs or proteins, the peptide mapping results also showed high consistency. Conclusions Cys exhibits a reducing ability comparable to DTT and possesses the advantageous characteristics of being nontoxic and odorless, making it a potential alternative for disulfide bond reduction and peptide mapping analysis of proteins and mAbs.
研究领域:
生物化学
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蛋白质组学
·
药物分析
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）