ULTRASONICS SONOCHEMISTRY
·
2022
·
IF 9.336
Yuxin Liu,Wang Zhe,Ruifen Zhang,Ziting Peng,Yuxi Wang,Heqi Gao,Zhiqiang Guo,Juan Xiao
Ultrasonic-assisted extraction (UAE) coupled with deep eutectic solvent (DES) is a novel, efficient and green extraction method for phytochemicals. In this study, the effects of 16 DESs coupled with UAE on the extraction rate of polyphenols from Paederia scandens (Lour.) Merr. (P. scandens), an edible and medicinal herb, were investigated. DES synthesised with choline chloride and ethylene glycol at a 1:2 M ratio resulted in the highest extractability. Moreover, the effects of extraction parameters were investigated by using a two-level factorial experiment followed by response surface methodology The optimal parameters (water content in DES of 49.2%, the actual ultrasonic power of 72.4 W, and ultrasonic time of 9.7 min) resulted in the optimal total flavonoid content (TFC) (27.04 mg CE/g DW), ferric-reducing antioxidant power (FRAP) value (373.27 μmol Fe(Ⅱ)E/g DW) and 2,2′-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS + ) value (48.64 μmol TE/g DW), closely matching the experimental results. Furthermore, a comparison study demonstrated that DES-UAE afforded the higher TFC and FRAP value than traditional extraction methods. 36 individual polyphenolic compounds were identified and quantified by ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) in P. scandens extracts, and of which 30 were found in the extracts obtained by DES-UAE. Additionally, DES-UAE afforded the highest sum of individual polyphenolic compound content. These results revealed that DES-UAE enhanced the extraction efficiency for polyphenols and provided a scientific basis for further processing and utilization of P. scandens .
研究领域:
植物化学
·
食品科学
·
药用植物学
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DOI
PubMed
高影响因子
Journal of Functional Foods
·
2017
·
IF 3.144
Han Peng,Wenting Li,Hongyan Li,Zeyuan Deng,Bing Zhang
Both extractable (EPE) and non-extractable bound phenolic (BPE) in the seed coat and cotyledon of black bean were investigated in the present study. Their total phenolic contents (TPC) and antioxidant activities (DPPH, ABTS and FRAP) were evaluated, and the phytochemical compositions of EPE and BPE in both seed coat and cotyledon of black soybean were identified and quantified ( Glycinemax(L.) merr ) by UPLC-ESI-QTOF-MS/MS and UPLC-ESI-QqQ-MS/MS. In the present study, a total of 112 and 78 compounds were identified or tentatively characterized in the black soybean EPE and BPE, respectively, with the majority being phenolic acids and flavonoids. Seed coat contains more abundant phenolics than that of cotyledon in all fractions. In addition, our results indicated that acid hydrolysis is more thorough than alkaline hydrolysis in the extraction of bound phenolics of black soybean. Our study provides available information for non-extractable bound phenolic constituents and their distribution in the seed coat and cotyledon of black soybean.
研究领域:
植物化学
·
食品科学
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产品关联
DOI
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 37
·30456-30465
·
IF 4.6
Jiangpeng Tang,Chang-ye Hui,Shunyu Hu,Wanyan Wu,Peishuo Cao,Jingwen Ling,Xueqin Yang,Zhenlie Huang,Yan Guo
Rapid, low-cost trace inorganic Hg(II) detection in environmental waters remains a critical public-health challenge. Here, we engineered Escherichia coli into a naked-eye whole-cell biosensor by coupling a redesigned MerR-Pmer element to the pyomelanin biosynthetic pathway. Three 4-hydroxyphenylpyruvate dioxygenase (HppD) homologs from Aeromonas media WS, Aeromonas hydrophila 4AK4, and Pseudomonas aeruginosa PAO1 were codon-optimized and functionally screened. The sensor strain of TOP10/pHg-PAO1 exhibited the broadest quantitative range (4.9–1250 nM) and the lowest detection limit (1.2 nM), outperforming most fluorescent counterparts. The water-soluble red-brown pyomelanin product was measured directly in the culture supernatants by absorbance at 400 nm, without requiring extraction. It remained chromogenically stable for at least 14 hours. The biosensor demonstrated absolute selectivity for Hg(II) in the presence of other ions, including Mg(II), Ca(II), Cd(II), Mn(II), Cu(II), Pb(II), and Zn(II), all at a concentration of 5 μM. It retained quantitative accuracy in tap, lake, and coastal seawater matrices. These features enable on-site, equipment-free screening of environmental waters and establish pyomelanin as a robust, user-friendly chromogenic reporter for next-generation whole-cell sensors.
研究领域:
环境科学
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合成生物学
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生物传感器
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微生物工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 4
·2635-2648
·
IF 3.9
Ying Li,Yingxin Tian,Qixue Wang,Xinyi Gu,Long Chen,Yiqun Jia,Shan Cao,Ting Zhang,Mingmei Zhou,Xiaojun Gou
Gynura segetum (Lour.) Merr. (GS), has been widely used in Chinese folk medicine and can promote circulation, relieve pain and remove stasis. In recent years, the hepatotoxicity caused by GS has been reported, however its mechanism is not fully elucidated. Metabolomic techniques are powerful means to explore the toxicological mechanism and therapeutic effects of traditional Chinese medicine. The purpose of this study was to establish a serum metabolomics method based on Gas Chromatography-Mass Spectrometry (GC-MS) to explore the hepatotoxicity mechanism of different exposure times and doses of GS in rats. Sprague Dawley (SD) rats were administered daily with distilled water, 7.5 g kg−1 GS, or 15 g kg−1 GS by intragastrical gavage for either 10 or 21 days. The methods adopted included enzyme-linked immunosorbent assay (ELISA), Hematoxylin and Eosin (H&E) staining and GC-MS-based serum metabolomics. Serum biochemistry analysis showed that the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), total bilirubin (TBIL) and total bile acid (TBA) significantly (P < 0.05) increased while the levels of albumin (ALB) and high-density lipoprotein (HDL) significantly (P < 0.05) decreased in GS-treated groups, compared with the control group. Interestingly, the ALT, AST, TG and ALB levels changed in a time- and dose-dependent manner. The results of H&E staining showed the degree of liver damage after administration of GS gradually deepened with the extension of administration time and the increase of the dose. According to the results of metabolomics analysis, 26 differential metabolites were identified, which were involved in 8 metabolic pathways including phenylalanine metabolism, glyoxylic acid and dicarboxylic acid metabolism and so on. Meanwhile, the number of differential metabolites in different GS-treated groups was associated with GS exposure time and dose. Therefore, we concluded that GS might induce hepatotoxicity depending on the exposure time and dose.
研究领域:
药理学
·
毒理学
·
代谢组学
·
传统中药
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ESSENTIAL OIL RESEARCH
·
2013
·
IF 0.553
Xiao-wei Huang,Yun-chao Feng,Yi Huang,Hai-ling Li
Clove oil and citronella oil from China were selected to evaluate their potential as skin-care ingredients. The gas chromatography–mass spectrometry (GC–MS) results showed that the main component of clove oil was eugenol, while citronellal was the main compound of citronella oil. Clove oil exhibited prominent free radical scavenging activities (ic
50: 58 μg/mL for O2
−· and 8.5 μg/mL for ABTS+·) and strong inhibitory effect on lipid peroxidation, whilst citronella oil showed weaker antioxidative activities (ic
50: 150 μg/mL for O2
−· and 500 μg/mL for ABTS+·) and lipid peroxidation inhibitory efficiency. In addition, both oils revealed dose-dependence in protecting bovine serum albumin against UV–TiO2–NO2
− induced protein oxidation and tyrosine nitration injury. This study suggests that both clove oil and citronella oil could be used as new source of skin-care ingredient in the cosmetic industry.
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Lianfeng Luo,Jieqiong Wang,Meiqin Li,Yingbin Zhang,Yuwan Wang,Yongquan Xu,Haisheng Chen,Yan Zhu,Zhihui Feng,Junfeng Yin
With a distinctive aroma, Shanhuangpi (SHP), the fruit of the plant Clausena anisum-olens (Blanco) Merr., has been newly developed as a commercial fruit resource. The fresh fruit deteriorates rapidly due to its high respiration and metabolism. Therefore, an effective drying process is urgently needed for its preservation. The influence of two commonly used drying methods for plant materials, i.e., vacuum freeze-drying (VFD) and hot air drying (HAD) was evaluated on the aroma, taste, color, and volatile profiles of this fruit. The results indicated that the VFD process well retained the color and volatile composition of the fresh fruit but produced extra graininess and sticky mouthfeel. Alternatively, the HAD process offered a shorter drying time, kept a more palatable sour/sweet ratio, but created a darker brown color and shrinking texture of the fruit. The volatile components were enriched by the micro-chamber/thermal extractor (μ-CTE) and 38 compounds were identified with gas chromatography-mass spectrometry. Sixteen odor-active compounds (detection frequency ≥4) were detected by gas chromatography–olfactometry. Results obtained in this study can be served as a reference for the evaluation and further development of SHP.
研究领域:
食品科学
·
植物学
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产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2022
·Vol. 85
·期 9
·809-816
·
IF 2.213
Liu Yue,Lin Yuzi,Li Ping,Yang Huiwen,Huang Qingsong,Pan Yufang
Increasing numbers of studies suggest that short-chain fatty acids (SCFAs) are closely related to diabetes. However, the complexity of fecal pretreatment makes the detection of SCFAs in feces relatively difficult. In this study, we established a sample pretreatment protocol that involved no tedious steps, requiring only the grinding of appropriate amounts of feces into powder in liquid nitrogen. The SCFA content in feces was then detected using the headspace-gas chromatography (HS-GC) method. The method achieved good linearity ( R 2 ≥ 0.9940), and the limits of quantification (LOQs) and detection (LODs) of all investigated SCFAs ranged from 0.15 to 0.45 mM and 0.05 to 0.15 mM, respectively. The recoveries of the SCFAs ranged from 91.55 to 103.96% in mouse fecal samples. The HS-GC method was then applied to evaluate the effects of a neutral polysaccharide from Acanthopanax trifoliatus (ATP1-1) on the fecal SCFA content in streptozotocin (STZ)-induced diabetic mice. After 6 weeks of treatment with 140 mg/kg/d ATP1-1, the expression levels of acetic acid, propionic acid, butyric acid, isobutyric acid, isovaleric acid and valeric acid were increased by 215.4, 88.8, 95.3, 300.8, 260.7 and 158.0%, respectively, which indicated that the hypoglycemic effect of ATP1-1 may be related to its impact on SCFAs. Our simplified pretreatment of fecal samples is suitable for the analysis and detection of SCFAs in mouse feces by HS-GC.
研究领域:
生物化学
·
医学
·
肠道微生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）