ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2022
·Vol. 29
·期 19
·27677-27687
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IF 5.19
Long Yujiao,Li Hongna,Jin Hongmei,Ni Jinren
The chemical and biological mechanisms of electrochemical elimination of Microcystis aeruginosa ( M. aeruginosa ) using boron-doped diamond (BDD) anode were comparatively explored in three different electrolytes (chloride, sulfate, and phosphate solutions). The most efficient elimination of M. aeruginosa was observed in chloride solution, which was attributed to the greatest total long-lived oxidants from the favorable formation of active chlorine. Moreover, the high permeability of active chlorine resulted in profound intracellular damages to chlorophyll- a , microcystin-LR (MC-LR), superoxide dismutase (SOD) enzyme, and DNA in the chloride system. The change of membrane permeability and degradation of the released MC-LR induced by active chlorine were further confirmed by the increase of extracellular MC-LR in the initial 5 min and a complete decay in the subsequent 15 min, while the change in morphology of algae cells was insignificant from SEM images. In sulfate and phosphate electrolytes, membrane damages were much more pronounced based on lipid peroxidation observation, although changes in cell morphology was found more significant in phosphate system. The higher concentrations of oxidants (·OH, O 3 , H 2 O 2 , S 2 O 8 2− ) generated in sulfate than in phosphate solution explained the greater efficiency of electrochemical elimination of M. aeruginosa in the sulfate electrolyte in terms of changes of cell density, OD 680 , chlorophyll- a , MC-LR, lipids, SOD enzyme, and DNA.
研究领域:
环境科学
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电化学
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水生生物学
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毒理学
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PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2021
·Vol. 107
·期 1
·147-153
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IF 2.151
Zhang Rui,Dong Pengyue,Sun Hongwen,Liu Chunguang
To investigate the combined effects of excess boron (B) and high salinity on the growth of freshwater algal species, Chlorella vulgaris and Microcystis aeruginosa were cultured in the medium with different B and salinities. The results show that high levels of B and salinity inhibited the growth of the two algal species. For C. vulgaris , low levels of B can alleviate the growth inhibition induced by salinity, and low levels of salinity can also relieve the growth inhibition induced by B. In contrast, high levels of salinity have little effect on B toxicity, while high levels of B aggravate salinity stress. For M. aeruginosa , salinity aggravates B toxicity, regardless of salinity levels. B supply worsens salinity stress on M. aeruginosa , regardless of supply doses. These results suggest that it may be possible to control algal bloom by regulating B or salinities.
研究领域:
生态学
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环境科学
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DOI
PubMed
高影响因子
ACS ES&T Water
·
2025
·
IF 4.3
Jiahui Zhang,Mingliang Wu,Miao Yu,Yue He,YuXin Xi,Chenyulin Wang,Yun Pan
Taste and odor (T&O) issues in drinking water sources are common sensory concerns, with thioethers being major contributors to septic odors. Although algal metabolism is a known source of these odors, the mechanisms driving thioether production remain insufficiently explored. This study systematically analyzed the primary thioethers present in a reservoir-based drinking water source in southeastern China. Multiple algal species were cultured under near-natural conditions, both individually and in combination, to investigate the mechanisms of thioether release. The results revealed that dimethyl sulfide (DMS), reaching concentrations up to 2.54 μg/L, was primarily produced by Cyclotella (diatom). Dimethyl trisulfide (DMTS), with a peak concentration of 0.26 μg/L─exceeding its odor threshold by 26-fold─posed the highest odor risk and was mainly produced by Microcystis aeruginosa (cyanobacteria) and Cyclotella. Cyclotella emerged as the primary thioether-producing algae, particularly when coexisting with Microcystis aeruginosa. As the biomass of Microcystis aeruginosa increased, the growth of Cyclotella was inhibited and its proliferative capacity declined, resulting in a 32–54% increase in thioether release, whereas thioether production by Microcystis aeruginosa decreased by 17–76%. This study provides novel insights into algal interactions driving thioether release, guiding odor risk management in water sources.
研究领域:
水生生态学
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环境科学
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饮用水安全
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Haifeng Qian,Jiahui Xu,Tao Lu,Qi Zhang,Qian Qu,Zhaopeng Yang,Xiangliang Pan
Linoleic acid (LA), is the product of secondary metabolism secreted from Microcystis aeruginosa , and it exhibits allelopathic activity against eukaryotic algae. However, information about on the mechanisms associated with the inhibition of algal activity by LA is limited. In this study, Chlorella pyrenoidosa was treated with LA (20–120 μg L −1 ) for 4 days, and its growth inhibition and physiological responses were examined for potential toxic mechanisms. The photosynthetic efficiency of C. pyrenoidosa was inhibited by LA treatments, and the F v /F m parameter decreased significantly compared to that of controls; however, the photosynthetic pigment content did not change significantly. Peroxidase activity was enhanced, relieving oxidative damage in algae after LA treatments. However, superoxide dismutase and catalase were suppressed, ultimately leading to the aggravation of lipid peroxidation. Transcriptome-based gene expression analysis revealed that the 120 μg L −1 LA treatment significantly inhibited the transcription of genes related to photosynthesis, carbon metabolism, and amino acid metabolism in C. pyrenoidosa , suggesting that these genes might be key LA targets in C. pyrenoidosa . Moreover, the expression of genes involved in vitamin, lipid, nitrogen cycling, terpenoid, and ascorbate metabolism was also affected, suggesting that LA inhibits algal cell growth through multiple pathways. The identification of LA-responsive genes in C. pyrenoidosa provides new insight into LA stress responses in eukaryotic algae.
研究领域:
植物学
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生态学
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分子生物学
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PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 14
·5907-5916
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IF 3.67
Qiu Yongting,Wang Zhihong,Liu Feng,Liu Junxia,Tan Kaiting,Ji Ruibo
Allelopathy by hydrophytes can be utilized to control algal blooms. This study was conducted to investigate the allelopathic effect (inhibition) of Scenedesmus quadricauda on Microcystis flos-aquae . When M. flos-aquae was co-cultured with S. quadricauda , the secretion of high-MW biopolymer by M. flos-aquae was inhibited by S. quadricauda . We further identified the allelochemicals and found that 4-tert-butylpyrocatechol (TBC) was the main active ingredient that could inhibit the growth of M. flos-aquae . When the dose of TBC was larger than 0.2 mg/L, almost all of the M. flos-aquae died. Additionally, TBC was found to suppress the growth of M. flos-aquae by disturbing the synthesis and secretion of proteins and polysaccharides and harming the chlorophyll to affect the light harvesting of algal cells. Therefore, TBC has the potential for use as a potential and promising algaecide to restrain the biomass of M. flos-aquae .
研究领域:
水生生物学
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环境微生物学
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化学生态学
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藻类生理学
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生物防治
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DOI
高影响因子
CHEMOSPHERE
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2020
·
IF 5.778
Yixiao Wu,Liang Wan,Weihao Zhang,Huijun Ding,Wenfeng Yang
Here we report a set of experiments in which water blooming cyanobacteria Microcystis aeruginosa was repeatedly exposed to erythromycin . Growth inhibition increased with increasing erythromycin concentration (1–150 μg/L) upon first exposure. Maximum inhibition rate (76.06%), occurred under 150 μg/L erythromycin. Moreover, 96-h 50% effective concentration (EC 50 ) was 22.97 μg/L, indicating that the growth of M. aeruginosa was affected by erythromycin under common environmental concentrations. Photosynthesis was hindered by chlorophyll and photosystem II limitations. Malondialdehyde , reactive oxygen species , and superoxide dismutase contents increased significantly under certain concentrations of erythromycin, but superoxide dismutase was suppressed by 150 μg/L erythromycin. Synthesis of intracellular and extracellular microcystins was promoted by 10–60 and by 20–60 μg/L erythromycin, respectively, but both were inhibited by 100–150 μg/L. Principal component analysis and Pearson’s correlation revealed the accumulation of reactive oxygen species as the dominant mechanism of erythromycin toxicity to cells. M. aeruginosa repeatedly subjected to erythromycin exposure showed obvious resistance against the antibiotic , especially when treated twice with 60 μg/L erythromycin. The 96-h EC 50 was 81.29 μg/L. As compared to the first exposure to erythromycin, photosynthetic and antioxidant activities increased, while growth inhibition and oxidation stress decreased upon multiple exposures. Production and release of microcystins were enhanced by repeated exposure to the antibiotic. Thus, erythromycin persistence in water should be examined, as repeated exposure may lead to serious environmental and human health hazards.
研究领域:
环境毒理学
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藻类学
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水生生态学
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抗生素抗性
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水污染
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DOI
高影响因子
Journal of Water Process Engineering
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2020
·
IF 3.465
Chang Tian,Yan-Xia Zhao
Polytitanium coagulants have been recently developed and have received wide attention due to both strong coagulation capability and sludge recycling to produce valuable by-products, titanium dioxide (TiO 2 ). This study reports the performance of coagulation with emerging polytitanium salts, polytitanium chloride (PTC) and polytitanium sulfate (PTS), for the treatment of two common cyanobacteria ( Microcystis aeruginosa , MA and Microcystis wesenbergii , MW)-laden surface water, which has been barely reported. Of particular concern is the coagulant dosage and solution pH dependence of polytitanium coagulation, which are the main parameters influencing coagulation efficiency. The influence of basicity (B) of PTC and PTS on coagulation performance was simultaneously included. Results show the optimized B value of 1.5 with coagulant dosage of 30 mg/L and initial solution pH between 7–9 for both PTC and PTS coagulation, wherein obvious advantage of PTC was observed over PTS regardless of MA- or MW-laden surface water treatment. The PTC coagulation could achieve almost complete removal of algae (about 99.0 %) with synergic removal of organic matter with high-efficiency. Both PTC and PTS were more capable at alkaline solution pH conditions for turbidity and algae removal through sweep flocculation, while satisfied removal of the humic acid-representative organic matters was observed at acidic to neutral pH conditions by charge neutralization. Satisfied flocculation zones were determined according to synergic removal of algae, turbidity and organic matters, with most of the satisfied points distributed at solution pH 7–9 conditions. This study will provide theoretical basis and facilitate wide application of the polytitanium coagulation for algae-loaded surface water treatment.
研究领域:
环境工程
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水处理
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胶体与界面化学
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环境化学
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给排水科学与工程
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 55
·83262-83272
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IF 5.19
Zhou Xudong,Jiang Xincheng,Chen Jibao,Gao Pengcheng
The extensive use of tetracyclines in human and veterinary medicine causes contamination in the environment that could contribute to the spread of antibiotic-resistant bacteria or competition between species of phytoplankton. In this study, Microcystis aeruginosa (a bloom-forming cyanobacterium) and Chlamydomonas microsphaera (common green alga) were selected to test the effects of different concentrations of tetracyclines (tetracycline and oxytetracycline) in monoculture and co-culture. The results showed that compared with monoculture, the cell growth of C. microsphaera decreased significantly in co-culture treated with different concentrations of tetracycline and oxytetracycline. The ratios of inhibition of M. aeruginosa exposed to 0.1, 2, and 10 mg L −1 of tetracycline varied between 17.7 and 31.37% in co-culture compared with monoculture, while the cell growth of M. aeruginosa was enhanced by treatment with 0.1, 2, and 7.25 mg L −1 of oxytetracycline in co-culture. However, the cell growth of C. microsphaera was significantly inhibited by all the treatments in co-culture. With the treatment of tetracycline, the specific growth rate of M. aeruginosa was 0.36 to 0.31 day −1 in monoculture and co-culture, while that of C. microsphaera ranged from 0.38 to 0.26 day −1 in monoculture, and it decreased from 0.25 day −1 (0 mg L −1 ) to 0.08 day −1 (10 mg L −1 ) in co-culture. With the treatment of oxytetracycline, the specific growth rate of M. aeruginosa was stimulated in co-culture, while that of C. microsphaera was significantly inhibited in co-culture compared with monoculture. Therefore, although M. aeruginosa significantly inhibited C. microsphaera in co-culture with the tetracycline-free treatment, the competitive advantage of M. aeruginosa expanded following the addition of low or high concentrations of tetracyclines.
研究领域:
环境科学
·
微生物学
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 15
·45388-45397
·
IF 5.8
Li Min,Wang Yayao,Xiao Jianxing,Yan Xingfu,Liu Bingru
Allelochemicals are essential agents for the biological control of harmful blooms. It is crucial to identify efficient algal suppressors and understand their mechanisms. This study reports the inhibition of Microcystis aeruginosa growth by 6 phenolic acids derived from plants’ secondary metabolites. The inhibitory effect of phenolic acids was significantly influenced by exposure dose and phenolic acid species. Caffeic acid has the most efficient algal inhibition ability (96 h-EC 50 of 5.8 mg/L). In contrast, the other 5 analogs (cinnamic acid, p -coumaric acid, 3-hydroxycinnamic acid, ferulic acid, and isoferulic acid) showed a weak inhibition effect or promotion effect with the exposure dose of 5–100 mg/L. ROS and chlorophyll a content tests combined with metabolomics analysis revealed that caffeic acid could induce the ROS accumulation of M. aeruginosa . They mainly disturbed nucleotide, amino acid, and fatty acid metabolism, leading to the downregulation of most metabolites, including toxins of microcystin LR and cyanopeptolin A, and the precursors of some unpleasant terpenoids. It has been suggested that caffeic acid is an effective agent for controlling M. aeruginosa blooms.
研究领域:
环境科学
·
水生生态学
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植物化学
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毒理学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Li Yin,Jinyu Han,Kaipian Shi,Ziyu Rong,Yong Zhang,Jixiang Fan,Huan He,Shaogui Yang,Lixiao Ni,Shiyin Li
Plant-derived allelochemicals are increasingly explored for cyanobacterial bloom control, but their effects on cyanotoxin accumulation and partitioning remain insufficiently understood. This study investigated the effects of quercetin on growth, photosynthetic performance, microcystin-LR (MC-LR) dynamics, and proteomic responses in Microcystis aeruginosa ( M. aeruginosa ). At low concentrations (≤ 1 mg/L), quercetin caused limited effects on cell density, while increasing extracellular MC-LR at selected sampling times. At growth-inhibitory concentrations (10–50 mg/L), quercetin suppressed algal growth and total MC-LR production, whereas the extracellular MC-LR fraction varied over time. At algicidal concentrations (≥ 70 mg/L), quercetin caused severe growth inhibition, impaired photosynthetic performance, and substantial leakage of intracellular MC-LR, resulting in persistent extracellular MC-LR risk. Short-term exposure further showed that quercetin rapidly altered intracellular and extracellular MC-LR partitioning. Proteomic analysis after 5 d of exposure to 50 mg/L quercetin revealed increased abundance of proteins involved in membrane transport, nutrient acquisition, and stress defense, together with decreased abundance of proteins associated with photosynthetic electron transport, carbon fixation, ribosomal function, and amino acid biosynthesis. Glutathione-related proteins were also upregulated, suggesting enhanced redox defense. Overall, these findings indicate that quercetin alters MC-LR partitioning of M. aeruginosa . This highlights the need to evaluate flavonoid-based algicides in terms of both bloom suppression and toxin-release risk.
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DOI
高影响因子
Journal of Oceanology and Limnology
·
2020
·Vol. 39
·期 1
·160-172
·
IF 0.617
Zhou Xudong,Jiang Xincheng,Gao Shan,Wan Zhenjia,Gao Pengcheng
The increasing use of pharmaceuticals has become a major environmental issue in China. The presence of antibiotics in water may have deleterious effects on non-target aquatic organisms such as microalgae. In this study, a cyanobacterium and an alga species in surface waters, Microcystis aeruginosa and Chlamydomonas microsphaera , were exposed to 0, 0.1, 0.5, 1.0, 2.0, 5.0, 10.0, and 20.0 mg/L of oxytetracycline dihydrate (OXY) and sulfamethoxazole (SMZ) for 96 h to determine the effects of these antibiotics on the growth and surface morphology. Moreover, the photosynthetic activity and the contents of superoxide dismutase (SOD), malondialdehyde (MDA), and protein were measured to examine the biochemical characteristics of M. aeruginosa and C. microsphaera under OXY and SMZ stress. The effects of both antibiotics on the growth of both species were concentration-dependent and characterized by low-dose stimulation and high-dose inhibition. C. microsphaera was more sensitive to both antibiotics than M. aeruginosa was. The algal cell membranes of both species disintegrated after exposure to a high concentration of OXY. All of the physiological parameters measured in this study were relatively stable at low concentrations of OXY and SMZ. After exposure to high concentrations of OXY and SMZ, photosynthetic activity decreased significantly, whereas lipid peroxidation and the abundance of SOD, MDA, and protein increased significantly. Thus, low-dose antibiotics may increase algal blooms in eutrophic waters.
研究领域:
环境科学
·
水生生物学
·
药理学
·
毒理学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2020
·
IF 7.864
Jie Mao,Xiaoqiang An,Zhenao Gu,Jing Zhou,Huijuan Liu,Jiuhui Qu
Exploring photoactive biotic–abiotic conjugations is of great importance for a variety of applications, but it remains difficult to probe the interfacial transfer of photoinduced charge carriers. In this work, Kelvin probe force microscopy, together with fluorescence imaging technique, were used to visually observe the spatial distribution and interfacial behavior of photocarriers in Microcystis aeruginosa/TiO2 hybrids. Experimental investigations suggested that photosynthetic microalgae cells were prone to trap photoholes from TiO2 photocatalysts. Oxygen vacancy defects in semiconductor exhibited significant impact on the charge migration, as the surface photovoltage of hydrogenated TiO2/microalgae hybrid was much higher than the pristine system. Profiting from the bioenhanced charge separation, biotic–abiotic architecture presented remarkably increased activity for photocatalytic inactivation of microalgae microorganisms. This work not only highlights the visual techniques for understanding the charge transfer around biotic–abiotic interface, but also provides a bioenhanced conjugation for the photocatalytic elimination of microorganisms in water treatment applications.
研究领域:
环境科学
·
材料科学
·
微生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）