COLLOIDS AND SURFACES B-BIOINTERFACES
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2015
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IF 4.152
Jixiang Zhu,Fanwen Yang,Fupo He,Xiumei Tian,Shuo Tang,Xiaoming Chen
A tubular gelatin scaffold for the time-dependent controlled release of epidermal growth factor (EGF) and mitomycin C (MMC) was fabricated. EGF was incorporated using silk fibroin carriers, and MMC was planted using polylactide (PLA) microspheres. The relationship between scaffold properties and crosslinking degrees was evaluated. As the crosslinking degree was increased from 23.7% to 65.3%, the mechanical properties of the scaffold obviously improved, and the compressive modulus increased to approximately 65 kPa. The mass degradation of the scaffold was also controlled from 9 days to approximately 1 month. In vitro release tests indicated that the scaffold mainly released EGF in the early period and MMC in the later period. Urethral epithelial cells (UECs) and urethral scar derived fibroblast cells (UFCs) were coseeded in the scaffold at a ratio of 1:1. After 9 days of coculture, immunostaining results displayed that the proportion of UECs continuously increased to approximately 71%. These changes in cell proportion were confirmed by the results of Western blot analysis. Therefore, the scaffold promoted the growth but inhibited the regeneration of UFCs. This scaffold for time-dependent controlled release of multiple biofactors may be potentially useful in urethral reconstruction and other tissue engineering studies.
研究领域:
生物医学工程
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组织工程
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药理学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2019
·
IF 2.938
Yuanyuan Zhang,Yuanyuan Zhao,Xin Gao,Weiwei Jiang,Zewen Li,Quancai Yao,Fengke Yang,Fanye Wang,Junhong Liu
The present study investigates the kinetic model of the enzymatic Michael addition of butylamine to 2-methyl-1,4-benzoquinone to form 2-methyl-3 -n- butylaminoyl-1-hydro-4-quinone in citrate buffer solution (pH 7.0). The yield of the product of 98% was achieved, mainly due to the excellent regioselectivity of immobilized lipase from T. laibacchii. The immobilized preparation used here was obtained by a method of purification and in situ immobilization. Through the purification using a PEG 4000/ K 2 HPO 4 aqueous two-phase system (ATPS), the T. laibacchii lipase was partitioned predominantly in the PEG-rich top phase where diatomite was added to achieve in situ immobilization via interfacial activation on the hydrophobic support. A proposed reaction mechanism of the Michael addition involves (1) the oxyanion hole polarizes the α,β-unsaturated carbonyl of 2-methyl-1,4 -benzoquinone, increasing its electrophilic ability, (2) the catalytic histidine deprotonates the nucleophile n-butyl amine. A modified sequential mechanism including ordered and random sequential bi-bi was proposed for the first, and it is beneficial to add these modification mechanisms to the family of enzyme complex reaction mechanism because the mechanism is partly expanded. The kinetic parameters were directly obtained by combining the numerical integration toolbox ode45 to solve differential equations and the nonlinear optimization toolbox fmincon for error minimizing objective function. A very satisfactory agreement between experimental data and model results was obtained based on the modified random bi-bi mechanism, implying that the enzymatic Michael addition may follow the modified random bi-bi mechanism. The mass transfer limitations were investigated, and it is found that both internal and external mass transfer limitations could be ignored.
研究领域:
生物化学
·
酶工程
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产品关联
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2022
·Vol. 29
·期 5
·1489-1505
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IF 2.523
Chang Ziling,Li Huafei,Yang Yuxiang,Yuan Hongming,Huang Yan,Yao Pingping,Ni Chaoying
Herein, we developed the dual-function template method to fabricate hollow magnetic nano-spheres (denoted as HMFe–Si–C n , n = 16, 18) with a mesoporous shell and hollow interior structure using alkyl chain trimethoxysilane templating. The shorter chain template directed formation of HMFe–Si–C 16 with size of 119 nm, having disordered inkbottle type mesopores and saturation magnetization of 50.01 emu/g higher than that of HMFe–Si–C 18 with cylindrical type mesopores. In addition, the HMFe–C n loaded with MMC diaplays a pH and magnetic dual responsive drug release behavior. By contrast, Mitomycin C (MMC) loading efficiency of HMFe–Si–C 16 was higher owing to the fact that the pore size, surface area, and pore volume of HMFe–Si–C 16 were larger than those of HMFe–Si–C 18 . Besides, MMC loaded HMFe–Si–C n hollow spheres showed a clear pH-dependent drug release behavior, having a higher release rate in acidic environments of pH 5.7. For the pH 5.7 and 7.4 release, the release kinetic for HMFe–Si–C 16 –MMC composites follows pseudo-first-order attributable to its special pore structure. With Fe(0) as a core, HMFe–Si–C n is distinguished for higher magnetic properties, and it is more conducive to magnetic targeted treatment of cancer cells and can release drugs through external magnetic field. In addition, HMFe–Si–C n has lower cytotoxicity and better biocompatibility. For this reason the inner cavity of HMFe–Si–C 16 could be labeled with radioisotope 99 Tc m to study the magnetic targeting distribution of HMFe–Si–C 16 in vivo, and its cytotoxicity against in vitro HeLa cells was also studied. These results indicate the potential of HMFe–Si–C 16 in the magnetic targeted drug delivery system.
研究领域:
纳米材料
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药物递送系统
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肿瘤治疗
✓ 已验证
产品关联
DOI
高影响因子
Materials Express
·
2023
·Vol. 13
·期 1
·81-84
·
IF 0.7
Wang Wen-Wen,Yang Li-Jie,Guo Jun-Feng
Pancreatic cancer is a common tumor of the digestive system with a high degree of malignancy and poor prognosis. Its incidence has significantly increased worldwide in recent years. Currently, approximately 90% of patients cannot be treated surgically. Thus, drug therapy plays an important
role in pancreatic cancer treatment. In this study, hydrogels loaded with mitomycin were prepared from hyaluronic acid and carboxymethyl chitosan, which have excellent biocompatibility. SEM results showed that the hydrogels had a three-dimensional interoperable porous structure, and CCK-8
results showed that hydrogels loaded with mitomycin significantly reduced cancer-cell proliferation. The hydrogels also reduced the migration of cancer cells in a dose-dependent manner, indicating their potential use as a drug-carrier material for pancreatic cancer treatment.
研究领域:
肿瘤学
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药剂学
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产品关联
DOI
高影响因子
EXPERIMENTAL EYE RESEARCH
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2020
·
IF 3.011
Mengliang Wu,Shangfeng Wang,Yan Wang,Fan Zhang,Tingting Shao
Pterygium is a degenerative disease that characterized by excessive fibrovascular proliferation. To reduce the recurrence rate, surgery is the main strategy, in combination with adjacent procedures or adjunctive therapy. One of the most common adjunctive agents, mitomycin C (MMC), is known as an alkylating agent that inhibits fibroblast proliferation but is limitedly applied in pterygium due to various complications. A previous study demonstrated that activated pterygium subconjunctival fibroblasts overexpressed low-density lipoprotein (LDL) receptors. In this study, we designed and synthesized MMC-loaded mesoporous silica nanoparticles conjugated with LDL (MMC@MSNs-LDL) to deliver MMC into activated pterygium fibroblasts in a targeted manner. The MMC loading efficiency was approximately 6%. The cell viability test (CCK-8 assay) revealed no cytotoxicity for the empty carrier MSNs at a concentration of ≤1 mg/ml after administration for 48 h in subconjunctival fibroblasts. Primary pterygium and normal human subconjunctival fibroblasts with or without stimulation by vascular endothelial growth factor (VEGF) were treated as follows: 1) 10 μg/ml MMC@MSNs-LDL for 24 h (MMC concentration: 0.6 μg/ml); 2) 0.2 mg/ml MMC for 5 min then cultured for 24 h after MMC removal; and 3) normal culture without any drug treatment. At 24 h, the anti-proliferative effect of MMC@MSNs-LDL in activated pterygium fibroblasts was similar to that of MMC (cell viability: 46.2 ± 5.5% vs 40.5 ± 1.1%, respectively, P = 0.349). Furthermore, the cytotoxicity of MMC@MSNs-LDL to normal fibroblasts with or without VEGF stimulation was significantly lower than that of traditional MMC (cell viability: 75.6 ± 4.4% vs 36.0 ± 1.5%, respectively, P < 0.001; 84.7 ± 5.5% vs 35.7 ± 1.3%, P < 0.001). The binding of fluorescently labeled MMC@MSNs-LDL in fibroblasts was assessed using confocal fluorescence microscopy . The uptake of targeted nanoparticles in fibroblasts was time dependent and saturated at 6 h. VEGF-activated pterygium fibroblasts showed more uptake of MMC@MSNs-LDL than normal fibroblasts with or without VEGF activation (both P < 0.001). Our data strongly suggest that MMC@MSNs-LDL had an effective antiproliferative role in activated pterygium fibroblasts, with reduced toxicity to normal fibroblasts compared to traditional application of MMC. LDL-mediated drug delivery might have great potential in the management of pterygium recurrence.
研究领域:
眼科
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药剂学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
Experimental and Therapeutic Medicine
·
2014
·Vol. 8
·期 3
·935-942
·
IF 0.941
Wei Zhang,Jiabo Hu,Quanhui Ma,Sanqiang Hu,Yanyan Wang,Xiangmei Wen,Yongbin Ma,Hong Xu,Hui Qian,Wenrong Xu
An immortalized mouse fetal liver stromal cell line, named KM3, has demonstrated the potential to support the growth and maintenance of human embryonic stem cells (hESCs). In this study, the characteristics of KM3 cells were examined following cryopreservation at -70˚C and in liquid nitrogen for 15, 30 and 60 days following treatment with 10 µg/ml mitomycin C. In addition, whether the KM3 cells were suitable for use as feeder cells to support the growth of hESCs was evaluated. The inhibition of mitosis without cell death was observed when the KM3 cells were treated with 10 µg/ml mitomycin C for 2 h. The morphology of the KM3 cells cryopreserved in liquid nitrogen for 60 days was not markedly changed, and the cell survival rate was 84.60±1.14%. By contrast, the survival rate of the KM3 cells was 66.40±2.88% following cryopreservation at -70˚C for 60 days; the cells readily detached, were maintained for a shorter time, and had a reduced expression level of basic fibroblast growth factor. hESCs cultured on KM3 cells cryopreserved in liquid nitrogen for 60 days showed the typical bird's nest structure, with clear boundaries and a differentiation rate of 16.33±2.08%. The differentiation rate of hESCs cultured on KM3 cells cryopreserved at -70˚C for 60 days was 37.67±3.51%. These results indicate that the cryopreserved KM3 cells treated with mitomycin C may be directly used in the subculture of hESCs, and the effect is relatively good with -70˚C short-term or liquid nitrogen cryopreservation.
研究领域:
干细胞生物学
·
细胞培养
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低温生物学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Haiyan Wu,Jianlei Wu,Nian Huang,Jean-François Masson,Haifeng Zhou,Wenjuan Zhou,Wei Lin,Guobin Liang
A sensitive ratiometric electrochemical strategy for MMC detection is developed. • Polythionine serves as an internal reference for improved response stability. • Ce-Ni-MOF/MWCNTs enhance interfacial electron transfer. • Molecular imprinting enables selective MMC recognition. • MMC detection is validated by UPLC-QTOF-MS in water samples. Mitomycin C (MMC) is a widely used antitumor antibiotic, and its rapid and selective detection is crucial for monitoring its occurrence in environmental water samples. In this work, a ratiometric electrochemical sensor was constructed by sequential modification of a bimetallic Ce-Ni-metal-organic framework (Ce-Ni-MOF)/multi-walled carbon nanotube (MWCNTs) composite, polythionine (pThi) and a molecularly imprinted polymer (MIP) on a glassy carbon electrode. The Ce-Ni-MOF/MWCNTs composite facilitated electron transfer, contributing to the enhanced sensitivity of the sensing platform. The pThi was electropolymerized onto the modified electrode surface to provide an internal reference signal, while the ratiometric response was achieved through two electrochemical channels, with the [Fe(CN)₆] 3−/4 − redox probe serving as the response signal and the pThi signal as the reference, enabling effective correction of measurement variations. Under optimized conditions, the sensor exhibited a linear response to MMC over the concentration range of 0.1–100 nM, with a low detection limit of 0.046 nM and a limit of quantification of 0.075 nM. Owing to the specific molecular recognition of the MIP layer and the internal reference function of pThi, the proposed sensor demonstrated satisfactory selectivity, repeatability, reproducibility and stability. Furthermore, the sensor was successfully applied to the determination of MMC in water samples, achieving recoveries of 87–112%. The analytical performance of the proposed sensor was further validated by comparison with the results obtained using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). This work presents a reliable and sensitive ratiometric electrochemical sensing strategy and demonstrates its feasibility through a proof-of-concept study for MMC detection in environmental water samples. Download: Download high-res image (304KB) Download: Download full-size image
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DOI
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Lunjie Zhao,Jianyou Xia,Congcong Yang,Jianping Tao,Shanfu Zhang,Wentao Hu,Chen Zhang,Jiuwu Zhang,Qiang Li,Yujie Xu
When the bladder is affected by factors such as tumors, tuberculosis, and trauma, its normal structure and function will be damaged inescapably. At this point, bladder replacement or enlargement surgery becomes necessary to restore its structure and improve the patient's quality of life. Traditional bladder reconstruction surgery, specifically ileum augmentation cystoplasty, has a complex surgical process, along with relatively large operative wounds and intestinal obstruction complications. There is an urgent need for bladder replacement material. Over the past decade, synthetic bladder scaffolds have emerged as alternatives; however, most fail to simultaneously address postoperative fibrosis and bacterial infection. To overcome these challenges, we developed an electrospinning poly (L-lactic acid)/gelatin (PLLA/GEL) microfiber membrane loaded with mitomycin C (MMC). By optimizing the PLLA/GEL ratio (7:3), the membrane had both good rigidity and excellent elasticity. The MMC-loaded fibers demonstrated sustained drug release, effectively inhibiting E. coli and S. aureus in vitro. In rat partial cystectomy models, the PLLA/GEL/MMC group showed reducing collagen III deposition through TGF-β/Smad2 pathway inhibition, enhancing urothelium regeneration and promoting angiogenesis. Notably, the membrane-maintained cell viability for both urothelial cells and smooth muscle cells over 7 days, confirming biocompatibility. These findings highlight a promising potential of the PLLA/GEL/MMC microfiber membrane not only as a material for bladder tissue engineering but also as a tool for therapeutic intervention that addresses multiple facets of bladder healing and regeneration.
研究领域:
生物医学工程
·
组织工程
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2016
·Vol. 9
·期 1
·334-340
·
IF 7.76
Meng Yang,Yanlei Liu,Wenxiu Hou,Xiao Zhi,Chunlei Zhang,Xinquan Jiang,Fei Pan,Yuming Yang,Jian Ni,Daxiang Cui
Human-induced pluripotent stem cells (iPS) possess an intrinsic tumor tropism ability. However, iPS cells are impeded in clinical applications of tumor therapy due to the formation of teratomas and their survival in normal organs such as the liver, lungs, spleen and kidneys. Mitomycin C (MMC) can overcome this limitation by suppressing iPS proliferation. Herein, we fabricated a safe delivery system of iPS cells treated with MMC loading with gold nanorods (AuNRs) for the targeted photothermal treatment of gastric cancer. Our results showed that the tumor cells were efficiently killed by the heat generated from the gold nanorods, and the iPS cells ultimately died due to the action of MMC seven days after the photothermal treatment. This suggested that pre-treated iPS cells with MMC can be used as a novel and safe approach for targeted tumor therapy. This paves the road for clinical translation in the future.
研究领域:
生物医学工程
·
癌症研究
·
纳米技术
·
干细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）