JOURNAL OF NEUROCHEMISTRY
·
2018
·Vol. 148
·期 4
·550-560
·
IF 4.609
Meisam Rostaminasab Dolatabad,Lu-lu Guo,Peng Xiao,Zhongliang Zhu,Qing-tao He,Du-xiao Yang,Chang-xiu Qu,Sheng-chao Guo,Xiao-lei Fu,Rui-rui Li,Lin Ge,Ke-jia Hu,Hong-da Liu,Yue-mao Shen,Xiao Yu,Jin-peng Sun,Peng-ju Zhang
s Protein Phosphatase Mg 2+ /Mn 2+ -Dependent 1K (PPM1K),also named as PP2Cm or branched-chain α-ketoacid dehydrogenase complex phosphatase, is a member of the metal-dependent phosphatase family and an important metabolic regulator. Single nucleotide polymorphisms (SNPs) in PPM1K contributing to protein functional defects have been found to be associated with numerous human diseases, such as cardiovascular disease, maple syrup urine disease, type 2 diabetes, and neurological disease. PPM1K N94K is an identified missense mutant produced by one of the SNPs in the human PPM1K coding sequence. However, the effects of the N94K mutant on its activity and structural property have not been defined. Here, we performed a detailed enzymological study using steady-state kinetics in the presence of pNPP or phospho-peptide substrates and crystallographic analyses of the wild-type and N94K PPM1K. The PPM1K-N94K significantly impaired its Mg 2+ -dependent catalytic activity and structural analysis demonstrated that the N94K mutation induced a conformational change in the key residue in coordinating the Mg 2+ in the active site. Specifically, three Mg 2+ were located in the active site of the PPM1K N94K instead of two Mg 2+ in the PPM1K wild type. Therefore, our results provide a structure basis for the metal ion-dependent PPM1K-N94K phosphatase activity.
研究领域:
生物化学
·
结构生物学
·
酶学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Laser & Photonics Reviews
·
2024
·
IF 9.8
Wei Liao,Xinquan Zhou,Jiance Jin,Yuzhen Wang,Zhiguo Xia
Thermochromic luminescent materials (TLMs) with spectral responsiveness to thermal stimuli have received broad attention due to the forefront applications in temperature visualization and sensing technologies. Herein, a phase diagram-driven strategy for designing TLMs in Cs–Cd–Br ternary system is presented. The room temperature stable Cs 7 Cd 3 Br 13 crystal is first obtained by utilizing a facile hydrothermal method, and the self-trapped exciton (STE) luminescence is improved by breaking local symmetry via Mg 2+ doping. Then thermochromic luminescence is further realized in Mn 2+ co-doped Cs 7 Cd 3 Br 13 on the basis of reversible structural transition to metastable Cs 2 CdBr 4 under thermal stimuli. The emission color change from bright yellow to green benefits from the variation of crystal fields around Mn 2+ , which can be switched by heating and cooling circulations. Finally, the resulting material is used as a demo to visualize the temperature of a homemade high-frequency induction heating sintering platform.
研究领域:
材料科学
·
光学工程
·
温度传感技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2021
·
IF 3.599
Kewei Ma,Yuhang Sheng,Guizhen Wang,Xiaowei Zhang,Yunsong Di,Cihui Liu,Liyan Yu,Lifeng Dong,Zhixing Gan
Mn 2+ doping has been developed to modulate the photoluminescence (PL) of CsPbCl 3 perovskite quantum dots (PQDs). However, the doped Mn 2+ may be expelled from the PQDs host with the long-term storing, resulting in inferior stability. Herein, CsPbCl 3 PQDs doped with different concentrations of Mn 2+ (Mn:CsPbCl 3 ) are prepared by a hot injection method. Our photostability tests reveal that when the doping concentration is <25%, or 40%–50%, the Mn 2+ doped PQDs are intrinsically stable. Anion exchange reactions are conducted between the stable CsPb 0.796 Mn 0.204 Cl 3 and CsPbBr 3 PQDs. Fluorescence color gamut of the resulting Mn:CsPb(Cl/Br) 3 PQDs almost covers the entire visible spectrum except for the deep red color. Benefiting from the intrinsic stability and wide color gamut, multicolor solid-state luminescence are acquired by incorporating the Mn:CsPb(Cl/Br) 3 PQDs in polymethyl methacrylate (PMMA) matrices, which are used as color converters for constructing light-emitting diodes (LEDs) with different colors.
研究领域:
材料科学
·
光电技术
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Xuesong Ye,Jun Nan,Zhencheng Ge,Qiliang Xiao,Bohan Liu,Meng Chen,Fangmin Wu
A novel mechanism for the effect of ferrous iron (Fe 2+ ) on low-pressure ultrafiltration membrane (LPM) flux is discovered in this study. In previous studies, the Fe 2+ concentrations of removing excessive Fe 2+ and manganese (Mn 2+ ) by LPM are low (0.4−1.5 mg L −1 ), and the presence of Fe 2+ would reduce the flux, thus not suitable for treating water with high Fe 2+ concentrations. In this study, we systematically investigated the performance of down-flow LPM (D-LPM) and opposite-flow LPM (O-LPM) under different Fe 2+ concentrations (0−5 mg L −1 ) with Mn 2+ and ammonia (NH 4 + ). The results demonstrate that the flux did decrease at low Fe 2+ concentration, while significantly increasing by 95.9% with Fe concentration at 3−5 mg L −1 . Furthermore, high Fe 2+ enhanced Fe, Mn 2+ , and NH 4 + removal by iron autocatalytic oxidation , enriching the relative abundance of manganese-oxidizing bacteria, and facilitating anammox activities, respectively. A series of characteristic analyses suggest that thin biofilm layer structures in D-LPM and O-LPM, along with the low soluble microbial products-polysaccharide concentrations, resulted in the high steady flux under high Fe 2+ concentrations. These findings widen the applicability of LPM in practical applications and provide novel strategies to increase the flux of LPM systems.
研究领域:
环境工程
·
水处理
·
膜技术
·
生物过程
✓ 已验证
产品关联
DOI
高影响因子
COMPUTERS IN BIOLOGY AND MEDICINE
·
2023
·
IF 7.7
Jinyi Qian,Yuzhou Wang,Xiner Liu,Zijian Hu,Nan Xu,Yuetong Wang,Tianqiong Shi,Chao Ye
Acetoin was widely used in food, medicine, and other industries, because of its unique fragrance. Bacillus amyloliquefaciens was recognized as a safe strain and a promising acetoin producer in fermentation. However, due to the complexity of its metabolic network, it had not been fully utilized. Therefore, a genome-scale metabolic network model ( i JYQ746) of B. amyloliquefaciens was constructed in this study, containing 746 genes, 1736 reactions, and 1611 metabolites. The results showed that Mg 2+ , Mn 2+ , and Fe 2+ have inhibitory effects on acetoin. When the stirring speed was 400 rpm, the maximum titer was 49.8 g L −1 . Minimization of metabolic adjustments (MOMA) was used to identify potential metabolic modification targets 2-oxoglutarate aminotransferase ( serC , EC 2.6.1.52) and glucose-6-phosphate isomerase ( pgi , EC 5.3.1.9). These targets could effectively accumulate acetoin by increasing pyruvate content, and the acetoin synthesis rate was increased by 610% and 10%, respectively. This provides a theoretical basis for metabolic engineering to reasonably transform B. amyloliquefaciens and produce acetoin.
研究领域:
生物技术
·
代谢工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-22
·
IF 17.694
Wang Xuan,Liu Yingqi,Xue Chencheng,Hu Yan,Zhao Yuanyuan,Cai Kaiyong,Li Menghuan,Luo Zhong
cGAS-STING pathway is a key DNA-sensing machinery and emerges as a promising target to overcome the immunoresistance of solid tumors. Here we describe a bovine serum albumin (BSA)/ferritin-based nanoagonist incorporating manganese (II) ions and β-lapachone, which cooperatively activates cGAS-STING signaling in dendritic cells (DCs) to elicit robust adaptive antitumor immunity. Mn 2+ -anchored mannose-modified BSAs and β-lapachone-loaded ferritins are crosslinked to afford bioresponsive protein nanoassemblies, which dissociate into monodispersive protein units in acidic perivascular tumor microenvironment (TME), thus enabling enhanced tumor penetration and spatiotemporally controlled Mn 2+ and β-lapachone delivery to DCs and tumor cells, respectively. β-lapachone causes immunogenic tumor cell apoptosis and releases abundant dsDNA into TME, while Mn 2+ enhances the sensitivity of cGAS to dsDNA and augments STING signaling to trigger downstream immunostimulatory signals. The cGAS-STING nanoagonist enhances the tumor-specific T cell-mediated immune response against poorly immunogenic solid tumors in vivo, offering a robust approach for immunotherapy in the clinics.
研究领域:
免疫学
·
肿瘤学
·
纳米技术
·
药学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）