Frontiers in Nutrition
·
2024
·
IF 4
Wang Zilin,Tian Yang,Yang Min,Yang Junyan,Wang Yifan,Tao Liang,Sheng Jun,Shi Chongying
Background: In this study, deep eutectic solvents (DESs) combined with ultrasound-assisted extraction (UAE) were used to extract bioactive compounds from the leaves of Moringa oleifera Lam.Methods: The FT-IR method was used to analyze the structural characteristics of the DESs, and the extraction efficiencies of the DESs for total phenolic content (TPC) and total flavonoid content (TFC) were evaluated. The stability of the extracts under high temperature and UV radiation was assessed, and their antioxidant activity was investigated after undergoing in vitro simulated digestion.Results: The results show that the seven DESs extracted more TPC and TFC than did the 70% ethanol (36.27 ± 1.58 mg GAE/g, 23.09 ± 1.47 mg RT/g), and the extraction process of UAE-DES was optimized by selecting choline chloride: citric acid as the DES solvent, which has the highest extraction of TPC (86.92 ± 1.34 mg GAE/g) and TFC (49.73 ± 0.85 mg RT/g). The stability results indicated that the DES phenolic extracts were less stable when exposed to high temperature and UV radiation, indicating that DES extracts have better bioactivity. Moreover, after in vitro simulated digestion, the DES extract shows a higher DPPH free radical scavenging capacity (12.79 ± 3.88 mmol Trolox/g of DES extracts, 6.99 ± 4.02 mmol Trolox/g of ethanol extracts) and ferric ion reducing antioxidant power (62.61 ± 1.71 mmol Trolox/g of DES extracts, 55.07 ± 1.66 mmol Trolox/g of ethanol extracts) than ethanol extracts.Conclusion: This study confirmed that DESs are a new and environmentally friendly solvent that can be used for the extraction of phenolic compounds.
研究领域:
植物化学
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食品科学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Huan Du,Yingyun Hu,Kaixiang Lu,Tao Li,Yang Tian,Yongjin Hu
Folate is an essential vitamin for the human body and must be obtained exogenously. It is recognized that some microorganisms are capable of producing folates during growth, revealing possibilities for innovations of natural folate biofortified foods. The aim of this study was to identify potential folate-producing strains to enhance folate content in fermented Moringa oleifera leaves powder (MOLP). Although most of the tested strains exhibited folate production, there were significant differences among them in terms of both quantity and distribution, whether intracellular or extracellular. 10 strains with potential for folate production were chosen for enhancing the folate content of MOLP. The two most promising strains, Lactiplantibacillus sp. LP1 and Saccharomyces cerevisiae Y-J-1, demonstrated high folate levels up to 1940 and 2115 μg/100 g dry matter, respectively. These levels were 1.6 and 1.8-fold the initial value in MOLP. Additionally, the fermentation of MOLP not only enhanced its nutritional quality but also preserved its significant bioactivity. This study provides the initial understanding of how selected microorganisms can naturally enrich folates in fermented MOLP, presenting an alternative solution to address the widespread issue of folate deficiency while promoting human health.
研究领域:
营养学
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微生物学
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食品科学
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生物技术
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Lang Wang,Tao Fei,Xiang Li,Qin Sun,Xiaoze Liu,Lu Wang
This study employed novel green solvents, deep eutectic solvents (DES), for protein extraction from Moringa oleifera seeds. Two DESs systems including choline chloride-malic acid (ChCl-MA 2 ) and choline chloride-urea (ChCl-U) exhibited the highest protein extraction efficiency, reaching 31.415 % and 31.945 %, respectively. Molecular dynamics (MD) simulation results revealed that the DESs systems showed smaller Root Mean Square Deviation (RMSD), Solvent Accessible Surface Area (SASA), and Radius of Gyration (Rg) values, along with a significant number of hydrogen bonds. The enhanced extraction efficiency of DESs was attributed to the formation of intermolecular hydrogen bonds. Both hydrogen bonding and hydrophobic interactions promoted protein aggregation, thereby stabilizing the protein structure and preserving its integrity. Spectroscopic analyses further indicated that proteins extracted with DESs displayed a more stable and ordered structure. However, DES-extracted proteins exhibited lower surface hydrophobicity (2000–4000) and reduced solubility (40 %–50 %), which significantly compromised their functional properties. Although DES-treated proteins displayed a superior foaming capacity (57.333 %), their emulsifying properties (3.464 m 2 /g and 1.584 m 2 /g, respectively) were comparatively unsatisfactory. In conclusion, DES represent a promising green and efficient extraction method for protein extraction. This work provides valuable insights into DES-mediated protein extraction and offers guidance for the efficient extraction of high-quality proteins from Moringa oleifera seeds.
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高影响因子
AAPS PHARMSCITECH
·
2024
·Vol. 25
·期 7
·1-15
·
IF 3.4
Shi Feng,Gong Mingjie,Adu-Frimpong Michael,Jiang Xia,Wang Xiaowen,Hua Qinyang,Li Tingyuan,Li Jiaying,Yu Jiangnan,Toreniyazov Elmurat,Cao Xia,Wang Qilong,Xu Ximing
In this study, N, N '-bis {4- [(α-L- rhamnosyloxy) benzyl]} thiourea (PG-1), a phenolic glycoside compound was purified from Moringa seed. The PG-1 has attracted extensive attention due to its anti-cancer, antioxidant, anti-inflammatory and hypoglycemic properties. However, some of its physicochemical properties such as oral bioavailability has not been studied. Herein, a highly purified PG-1 was extracted and incorporated in multiple layered liposomes (PG-1-L) to avoid its burst release and enhance oral bioavailability. After appropriate characterization, it was discovered that the obtained PG-1-L was stable, homogeneous and well dispersed with the average particle size being 89.26 ± 0.23 nm. Importantly, the in vitro release and in vivo oral bioavailability of PG-1-L were significantly improved compared with PG-1. In addition, MTT results showed that compared with the free PG-1, PG-1-L displayed obvious inhibitory effect on the HepG2 cells, while the inhibitory effect on healthy non-malignant 3T6 and LO-2 cells was not significant, indicating that PG-1-L had high safety. In conclusion, PG-1-L can be used as a promising delivery system and an ideal novel approach to improve the oral bioavailability and anticancer activity of PG-1. Graphical Abstract
研究领域:
药理学
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纳米技术
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药剂学
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药物化学
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DOI
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-18
·
IF 3.9
Siddique Farzana,Ahmad Salman,Hussain Ashiq,Firdous Nida,Nisar Rizwan,Bilal Muhammad,Najam Ayesha,Salik Abdullah,Zia Muhammad,Elkhedir Abdeen Elsiddig
Yoghurt is a one of the commonly consumed nutritious dairy products, which lacks certain bioactive compounds that are present in abundance in many plants. While, a significant range of phytonutrients are present in the leaves of Moringa oleifera . Thus, the purpose of this work is to develop a functional yoghurt, which could promote the health of the consumers to its bioactive components. In the present work, the buffalo milk yoghurt supplemented with Moringa oleifera leaves powder [at 0% (T 0 ), 0.2% (T 1 ), 0.4% (T 2 ), 0.6% (T 3 ) and 0.8% (T 4 )] was prepared to check the variations in the physicochemical, bioactive, and antioxidant properties, during 21 days of refrigerated storage. The results showed that the fat content was decreased from T 0 (4.42%), to T 4 (3.37%), and decreased from 4.40 to 4.38% during storage period of 21 days. The highest protein content was observed in T 4 (4.15%), while the lowest in T 0 (3.61%), and this protein content also deceased during storage. The moisture content was high in T 0 and lowest in T 4 , while increased in all samples during the storage. The pH and total plate count decreased significantly from T 0 to T 4 , whereas with the storage the pH decreased and total plate count was increased. A significant increase in the TPC, TFC and antioxidant activity of yoghurt samples was observed, with increasing the level of incorporation of Moringa oleifera leaves powder, as T 4 presented the highest values for these parameters. However, as storage days passed from 0 to 21, the TPC, TFC and antioxidant activity of all the samples was significantly decreased. Minerals analysis also revealed a significant increase in the Ca, K, Na, Fe and Zn content of the yoghurt, as a result of increasing the level of Moringa oleifera leaves powder, as T 4 was found to be having the highest amounts of these minerals. Current study concludes that Moringa oleifera leaves powder could be an excellent fortification ingredient for the development of functional yoghurt, which due to the presence of bioactive compounds could provide pharmacological benefits.
研究领域:
食品科学
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营养学
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药理学
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乳品技术
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植物生物化学
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DOI
PubMed
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 9
·1847
·
IF 4.1
Bo Ao,Honglin Jiang,Xuan Cai,Decheng Liu,Junming Tu,Xiaoshan Shi,Yanxiang Wang,Fei He,Jing Lv,Jingjing Li,Yuanliang Hu,Xian Xia,Jianjun Hou
Today, pathogenic microorganisms are increasingly developing resistance to conventional drugs, necessitating the exploration of alternative strategies. In addressing this challenge, nano-based antibacterial agents offer a promising avenue of research. In the present study, we used an extract ofMoringa oleifera, a widely recognized edible and medicinal plant, to synthesize biogenetic tellurium nanoparticles (Bio-TeNPs). Transmission electron microscopy, scanning electron microscopy, and dynamic light scattering analyses revealed that the obtained Bio-TeNPs had diameters between 20 and 50 nm, and zeta potential values of 23.7 ± 3.3 mV. Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy revealed that the Bio-TeNPs consisted primarily of Te(0), along with some organic constituents. Remarkably, these Bio-TeNPs exhibited potent antibacterial activity against a spectrum of pathogens, includingEscherichia coli,Klebsiella pneumoniae,Shigella dysenteriae,Salmonella typhimurium,Streptococcus pneumoniae, andStreptococcus agalactiae. In addition, findings from growth curve experiments, live/dead cell staining, and scanning electron microscopy observations of cell morphology demonstrated that Bio-TeNPs at a concentration of 0.07 mg/mL effectively disruptedE.coliandK.pneumoniaecells, leading to cell rupture or shrinkage. The biofilm inhibition rates of 0.7 mg/mL Bio-TeNPs againstE.coliandK.pneumoniaereached 92% and 90%, respectively. In addition, 7 mg/mL Bio-TeNPs effectively eradicatedE.colifrom the surfaces of glass slides, with a 100% clearance rate. These outcomes underscore the exceptional antibacterial efficacy of Bio-TeNPs and highlight their potential as promising nanomaterials for combating bacterial infections.
研究领域:
纳米生物学
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微生物学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Tao Liang,Gu Fan,Liu Yan,Yang Min,Wu Xing-Zhong,Sheng Jun,Tian Yang
Moringa oleifera leaves are a kind of new food raw materials, rich in functional factors, M. oleifera leaves aqueous extract have antioxidant activity and M. oleifera leave protein is an important active ingredient in the aqueous extract. Numerous studies have shown that peptides have strong antioxidant activity. To reveal the antioxidant effects of M. oleifera (MO) leaves peptides, MO leave antioxidant peptides were isolated and prepared to clarify their antioxidant activity. MLPH1 (<1 kDa), MLPH3 (1~3 kDa), MLPH5 (3~5 kDa), and MLPH10 (5~10 kDa) fractions were obtained by the membrane ultrafiltration classification of MO leaves proteolytic hydrolysate (MLPH). MLPH1 was further separated by centrifugal filters, and the fraction separated by <1 kDa (MLPH1-1) was identified and analyzed by LC–MS/MS. The purpose of this study was to investigate the effect of MO leaves antioxidant peptide pretreatment on H2O2-treated HepG2 cells and to refine the antioxidant activity. The results showed that MLPH1 had the strongest antioxidant activity, and three MO leaves antioxidant peptides (LALPVYN, LHIAALVFQ, and FHEEDDAKLF) were obtained. The peptide with the sequence LALPVYN and a molecular weight of 788.44 Da had the strongest antioxidant activity. After 24 h of LALPVYN pretreatment, the cell viability and the CAT, GSH-Px, and SOD enzyme activity were significantly increased, and the MDA, ROS, and apoptosis rates were significantly decreased. These results provide a theoretical basis for further research on the antioxidant mechanism of MO leaves peptides.
研究领域:
生物化学
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药理学
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细胞生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Weilong Peng,Yaya Wang,Lijun Wang,Weimei Wang,Junjie Huang,Ruigang Zhou,Ruonan Bo,Cuiyan Liu,Mingjiang Liu,Jingui Li
This study aimed to develop a straightforward and efficient method for screening natural deep eutectic solvent (NADES) with high extraction yield of polysaccharides from Moringa oleifera Lam. ( M. oleifera ) leaves and to elucidate the solubilization mechanism of this eco-friendly extraction solvent. Traditional extraction methods are often limited by low efficiency, long processing times, and environmental hazards, which hinder the advancement of polysaccharide applications. In this study, the conductor-like screening model for realistic solvents (COSMO-RS) was adopted to prognosticate the solubility of biomolecules, and the M. oleifera polysaccharide (MOLP) solubility of 32 NADESs was appraised. Based on the solubility and activity coefficient results, we meticulously selected eight highly efficient NADESs combinations and conducted practical verification. The Bet-LA (Betaine- Lactic acid) combination demonstrated the highest extraction efficiency for MOLP, achieving an ultrasonic-assisted extraction rate 2.67-fold higher than that of the conventional ethanol solvent. The dissolution mechanism was further elucidated using frontier molecular orbital (FMO) and reduced density gradient (RDG) analyses. Antioxidant assays demonstrated that polysaccharides extracted by Bet-LA exhibited superior biological activity. These findings establish an efficient NADES screening protocol and provide mechanistic insights for optimizing M. oleifera leaves polysaccharide extraction.
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 20
·12405
·
IF 6.208
Fan Gu,Liang Tao,Runling Chen,Jiao Zhang,Xingzhong Wu,Min Yang,Jun Sheng,Yang Tian
Moringa oleiferaleaves (MOL) are a new food resource, rich in functional factors. MOL polysaccharides are important active macromolecules within MOL. However, there are problems, such as low extraction rates and lack of evidence for functional activity. Therefore, in this experiment, single-factor experiments were carried out using MOL powder as the raw material, and the Plackett–Burman test was used to screen the significantly influential test factors. The extraction process of MOL polysaccharide was optimized by response surface methodology. The insulin resistance alleviating activity of MOLP polysaccharides was initially explored. The results showed that the extraction ofMoringa oleiferaleaves crude polysaccharides (MOLP) by ultrasonic assisted cellulase enzymatic digestion was (17.03 ± 1.03)%, and the obtained MOLP was a crude polysaccharide with an average molecular weight (Mw) of 279.48 kDa, consisting of fucose, rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid, and glucuronic acid. MOLP had an IC50 value of 8.02 mg/mL for α-glucosidase and scavenging activity against free radicals such as ABTS, DPPH, hydroxyl radicals, and superoxide anion with an IC50 value of 0.21 mg/mL 0.31 mg/mL 0.97 mg/mL 0.49 mg/mL. At the same time, MOLP significantly enhanced the glucose consumption, glycogen synthesis, CAT, SOD, GSH-Px activity, and reduced the MDA and ROS content in high glucose-induced insulin-resistant HepG2 (IR-HepG2) cells. This experiment improved the extraction rate of MOLP and demonstrated that MOLP has antioxidant activity and α-glucosidase inhibitory activity, which can alleviate the insulin resistance of high glucose-induced HepG2 cells. It provides partial data support for the possible hypoglycemic effect of MOLP by alleviating oxidative stress, and also provides new ideas for the in-depth study of basic research and industrial application of MOLP.Keywords:Moringa oleiferaleaves crude polysaccharides;ultrasonic-cellulase extraction;response surface methodology;antioxidant;insulin resistance
研究领域:
食品科学
·
药理学
·
生物技术
·
生物化学
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DOI
PubMed
高影响因子
Desalination and Water Treatment
·
2024
·
IF 1
Jingxi Tie,Zhihong Zheng,Xiaomei Zheng,Yaru Hao
The water-soluble protein was extracted from Moringa oleifera seed press cake and tested its performance as a natural coagulant for removing humic acid (HA) from water solution when comparing to polyaluminum chloride (PAC), a commercial coagulant. Influencing factors including pH, coagulant dosage, temperature and suspended solid were investigated in this study. The results indicated that the coagulating efficiencies of HA using the Moringa oleifera seed protein (MOSP) were better than those by PAC under all the experimental conditions at the same dosage. MOSP could increase the zeta potential more efficiently than PAC. The Fourier transform infrared spectra revealed that no new compounds were generated during the coagulation process. Hence, adsorption and charge neutralization could be the main mechanism contributing to the HA removal using MOSP as coagulant. MOSP is an ideal natural coagulant for HA removal.
研究领域:
环境科学
·
水处理技术
·
天然产物应用
·
环境工程
·
胶体化学
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DOI
高影响因子
Frontiers in Pharmacology
·
2020
·
IF 4.225
Xie Jing,Luo Feng-xian,Shi Chong-ying,Jiang Wei-wei,Qian Ying-yan,Yang Ming-rong,Song Shuang,Dai Tian-yi,Peng Lei,Gao Xiao-yu,Tao Liang,Tian Yang,Sheng Jun
Moringa oleifera Lam. (M. oleifera) is valuable plant distributed in many tropical and subtropical countries. It has a number of medicinal uses and is highly nutritious. M. oleifera has been shown to inhibit tumor cell growth, but this effect has not been demonstrated on prostate cancer cells. In this study, we evaluated the inhibitory effect of M. oleifera alkaloids (MOA) on proliferation and migration of PC3 human prostate cancer cells in vitro and in vivo. Furthermore, we elucidated the mechanism of these effects. The results showed that MOA inhibited proliferation of PC3 cells and induced apoptosis and cell cycle arrest. Furthermore, MOA suppressed PC3 cell migration and inhibited the expression of matrix metalloproteinases (MMP)-9. In addition, MOA significantly downregulated the expression of cyclooxygenase 2 (COX-2), β-catenin, phosphorylated glycogen synthase 3β, and vascular endothelial growth factor, and suppressed production of prostaglandin E2 (PGE2). Furthermore, FH535 (β-catenin inhibitor) and MOA reversed PGE2-induced PC3 cell proliferation and migration, and the effects of MOA and FH535 were not additive. In vivo experiments showed that MOA (150 mg/kg) significantly inhibited growth of xenograft tumors in mice, and significantly reduced the protein expression levels of COX-2 and β-catenin in tumor tissues. These results indicate that MOA inhibits the proliferation and migration, and induces apoptosis and cell cycle arrest of PC3 cells. Additionally, MOA inhibits the proliferation and migration of PC3 cells through suppression of the COX-2 mediated Wnt/β-catenin signaling pathway.
研究领域:
肿瘤学
·
药理学
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细胞生物学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2017
·
IF 6.395
Yijin Cai,Ying Luo,Hanwu Dong,Xiao Zhao,Yong Xiao,Yeru Liang,Hang Hu,Yingliang Liu,Mingtao Zheng
A facile one-step pyrolysis route for the synthesis of hierarchically porous carbon nanosheets (PCNSs) derived from Moringa oleifera stems (MOSs) is reported, in which no post-activation-process in needed. The as-prepared PCNSs possesses unique porous nanosheet morphology with high specific surface area of ca. 2250 m 2 g −1 , large pore volume of ca. 2.3 cm 3 g −1 , appropriate porosity as well as heteroatom doping (N and O), endowing outstanding electrochemical properties as electrode material for high-performance supercapacitors . The PCNS-based electrodes are investigated in various aqueous electrolytes including 1.0 M Na 2 SO 4 , 1.0 M H 2 SO 4 , and 6.0 M KOH. The PCNSs exhibit a maximum specific capacitance of ca. 283 F g −1 (0.5 A g −1 ), excellent rate capability (ca. 72% of capacitance retention even at an ultrahigh current density of 50 A g −1 ), and a tremendous long-term cycling stability in the three-electrode system. Moreover, the as-assembled PCNS-based symmetric supercapacitor shows a high energy density of ca. 25.8 Wh kg −1 (in 1.0 M Na 2 SO 4 electrolyte) and remarkable long-term cycling stability (almost no capacitance fade in aqueous electrolytes), indicating the promising of the as-prepared PCNSs for electrochemical energy storage and conversion .
研究领域:
材料科学
·
能源存储
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）