MOLECULES
·
2018
·Vol. 23
·期 7
·1788
·
IF 3.098
Hong-Xin Cui,Ji-Hong Chen,Jing-Wan Li,Fang-Rong Cheng,Ke Yuan
Myrica rubra(MR) is rich in anthocyanins, and it has good anti-cancer, anti-aging, antioxidant, and antiviral effects. The proportion of disability and death caused by ischemic stroke gradually increased, becoming a major disease that is harmful to human health. However, research on effects of anthocyanin from MR on cerebral ischemia-reperfusion (I/R) injury is rare. In this study, we prepared eight purified anthocyanin extracts (PAEs) from different types of MR, and examined the amounts of total anthocyanin (TA) and cyanidin-3-O-glucoside (C-3-G). After one week of PAE treatment, the cerebral infarction volume, disease damage, and contents of nitric oxide and malondialdehyde were reduced, while the level of superoxide dismutase was increased in I/R mice. Altogether, our results show that Boqi1MR contained the most TA (22.07%) and C-3-G (21.28%), and that PAE isolated from Dongkui MR can protect the brain from I/R injury in mice, with the mechanism possibly related to the Toll-like receptor 4 (TLR4)/ nuclear factor-κB (NF-κB) and NOD-like receptor pyrin domain-containing 3 protein (NLRP3) pathways.Keywords:Myrica rubra;anthocyanins;cyanidin-3-O-glucoside;cerebral ischemia-reperfusion;TLR4/NF-κB;NLRP3
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2017
·
IF 2.329
Yu Zhang,Xingqian Ye,Zhimin Xu,Jie Duan,Chaoyang Wei,Guihua Xu,Shiguo Chen
Three different Chinese bayberry leaves proanthocyanidins (BLPs) extracts were prepared by a normal extraction method, Sephadex LH-20 column purification (SPBLPs) and ultrasound degradation (UDBLPs). Their peroxyl radical scavenging capacity (PSC) and anti-lipid oxidation activity were evaluated. SPBLPs had a high PSC value at 23000.4 ± 398.5 mg of vitamin C equivalent/100 g. An emulsion model containing cholesterol and linoleic acid was used to evaluate the anti-lipid oxidation ability of BLPs. The inhibition rates of 7-ketocholesterol for the UDBLPs and SPBLPs at 40 μg/mL were 83.8 ± 0.6% and 88.5 ± 0.4% respectively, which were equivalent to that of artificial antioxidant BHT or TBHQ at 20 μg/mL. SPBLPs exhibited great antioxidant activity in prevention of lipid oxidation. The prevention of linoleic acid oxidation was highly correlated to the total phenolic contents (TPC), while the correlation of the inhibition of cholesterol oxidation and the proanthocyanidins contents was higher than that of the TPC. Overall, UDBLPs or SPBLPs could be a natural antioxidant applied for health promotion.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 9
·6462-6472
·
IF 2.6
Tian Siyi,Chang Guoli,Xiang Yannan,Cai Chenggang,Luo Xinyu,Zhu Ruiyu,Yang Hailong,Gao Haiyan
In order to investigate the hypoglycaemic mechanism and potential applications of four hypoglycaemic flavonoid glycosides, namely myricitrin, Cyanidin-3-O-glucoside (C3G), hyperoside and quercitrin in Myrica rubra pomace, the stability of these four flavonoid glycosides and their binding mechanisms were studied using molecular docking. The results demonstrated that pH value affects on the stability of these four components in M. rubra pomace. C3G exhibited the most significant inhibitory effect on α-glucosidase at pH 5, with myricitrin, hyperoside and quercitrin showing the highest inhibitory effect at pH 7. Moreover, an increase in temperature and storage time reduced the inhibitory effect of these four glycosidic components on α-glucosidase. Molecular docking analysis revealed that myricitrin formed hydrogen bonds with the active site residues of α-glucosidase, namely Phe550, Ile552, Asp555, Ser574 and Arg576, and also engaged in hydrophobic interactions with Lys551. Hyperoside formed hydrogen bonds with α-glucosidase, formed hydrophobic interactions with Lys50 and exhibited π-cation interaction with Lys53. Quercitrin formed hydrogen bonds with α-glucosidase, formed hydrophobic interactions with Lys500 and established salt bridges with Lys50. C3G formed hydrogen bonds and hydrophobic interactions with α-glucosidase and showed π-π interactions with Phe301. These findings will provide valuable insights for the application of these four chemicals.
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Journal of Functional Foods
·
2016
·
IF 3.973
Yu Zhang,Shiguo Chen,Chaoyang Wei,Jianchu Chen,Xingqian Ye
The increasing rate of hyperlipidemia has drawn great attention and natural products may play a potential role in prevention of these disorders. Chinese bayberry leaves proanthocyanidin (BLPs) with epigallocatechin-3- O -gallate (EGCG) as its terminal and major extension units is unusual in the plant kingdom. Crude, purified and ultrasound depolymerized BLPs (CBLPs, SPBLPs and UDBLPs) were prepared with the aim to investigate their hypolipidemic mechanisms. BLPs inhibited the lipase activity in vitro and reduced the accumulation of total triacylglycerols (TAG) and cholesterol induced by oleic acid in HepG2 cells. SPBLPs and UDBLPS significantly increased the phosphorylation of AMP-activated protein kinase (AMPK) and thus reduced TAG and sterols biosynthesis by inhibiting its downstream proteins, such as acetyl-CoA carboxylase, 3-hydroxy-3-methylglutaryl-CoA reductase and sterol regulatory element-binding protein. BLPs also promoted glucose consumption in oleic acid induced HepG2 cells. Overall, this study suggests the hypolipidemic property and promotion of glucose consumption of BLPs via AMPK pathway.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yini Fu,Yunyun Zheng,Baozhu Shi,Bingge Liu,Ming Zhang,Yanyun Zhu
In this study, we used Chinese bayberry residue as the raw material to extract anthocyanins using deep eutectic solvents (DESs). We screened for an efficient ADES—betaine & citric acid (Bet-CA), and coupled it with ultrasound for the extraction of anthocyanins. We delved deeply into the relationship between the physicochemical properties of deep eutectic solvents and their extraction efficiency of anthocyanins, and studied its degradation kinetics under different temperatures and light conditions. This provides a green, efficient, and multi-stability protective strategy for the recovery of anthocyanins from plant-based by-products. The results revealed that the total anthocyanin content (TAC) extracted using HB47 was 7 times higher (7.05 mg CGE/g DW) than that extracted using the conventional acidified ethanol solvent (1.05 ± 0.05 mg CGE/g DW). Correlation analysis demonstrated a significant positive correlation ( p < 0.01) between TAC and a ⁎ . The extracts were subjected to heat treatment at different temperatures and stored different light conditions. HB47 exhibited the highest degradation rate (k = 0.1169) at 80 °C. However, it showed the greatest stability at lower temperatures, particularly at 4 °C (k = 0.0016, t ½ = 433.22 h). After 7 days of storage in darkness, the preservation rate of anthocyanins extracted by different solvent systems remained above 90 %. While the loss rate under UV light exposure reached as high as 95 %, significantly higher than that under sunlight.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Ru Fan,Jianxing Xiang,Peipei Zhou,He Mei,Yanyan Li,Huili Wang,Xiaodong Liu,Xuedong Wang
As one kind of high nutrition fruits, abandoned Myrica rubra causes great waste due to short storage period. For resource utilization, we herein fabricated the Myrica rubra- based N-doped carbon dots (MN-CDs) by a facile/green hydrothermal method. MN-CDs, fabricated from four regions of China, displayed significant differences in their corresponding fluorescence intensities (FIs). Interestingly, different batches of waxberry samples from the same region (Wenzhou, China) exhibited slight differences in their FIs, and also an excellent anti-photobleaching and anti-salt capacity. Based on Fe 3+ -triggered quenching effect and fluorescent recovery by redox reaction of AA and Fe 3+ , MN-CDs were employed to construct an “on-off-on” switch probe for sequential detection of Fe 3+ and ascorbic acid (AA). Through Zeta potential, UV spectrum, Stern-Volmer equation, and valence-conduction band theory, the Fe 3+ -triggered quenching belonged to a static quenching process, which resulted from the synergistic contribution of inner filtering effect and photo-induced electron transfer mechanisms. The linear ranges for Fe 3+ and AA detections were 1–1000 and 0.1–1000 mM. The limits of detection were 0.3 μM for Fe 3+ in environmental waters, and 0.03 μM for AA in pharmaceutical tablets and fruit juice samples. Under 365-nm UV lamp, the color changes of test papers were easily observed from dark blue and bright blue in the presence of Fe 3+ and AA, and thus the MN-CDs-based switch probe could be satisfactorily used for visually qualitative detection of Fe 3+ and AA outdoor with our naked eyes. To sum up, MN-CDs not only realize resource reutilization of abandoned Myrica rubra , but also offer an convenient outdoor approach for qualitative detection of Fe 3+ and AA in complex matrices.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-12
·
IF 5.3
Zhou Peipei,Hong Yangluchen,Fan Ru,Wang Huili,Wang Xuedong,Mei He
To realize the reutilization of waste Myrica rubra in the analytical field, we synthesized Myrica rubra –based N-doped carbon dots (MN-CDs) and further anchored them onto the surface of Fe 3 S 4 to fabricate Fe 3 S 4 @MN-CD nanocomposites. The as-fabricated nanocomposites possessed higher peroxidase-mimetic activity than its two precursors, resulting from the synergistic effect between them, and could catalyze colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into deep blue oxTMB with a strong 652-nm absorption. Under optimized conditions (initial solution pH, 3.5; incubation temperature, 35 ℃; Fe 3 S 4 @MN-CD concentration, 50 µg mL −1 , and 652-nm absorption), Fe 3 S 4 @MN-CDs were employed for colorimetric assay of p -aminophenol ( p -AP) with wide linear range (LR, 2.9–100 µM), low detection limit (LOD, 0.87 µM), and satisfactory recoveries (86.3–105%) in environmental waters. Encouragingly, this colorimetric assay provided the relative accuracy of 97.0–99.4% as compared with conventional HPLC-UV detection. A portable smartphone-based colorimetric application was developed by combining the Fe 3 S 4 @MN-CD-based visually chromogenic reaction with a “Thing Identify” APP software. Besides, we engineered an image-capturing device feasible for field use, in which the internal-compact sealing prevented external light source from entering photography chamber, thereby reducing light interference, and also the bottom light source enhanced the intensity of blue imaging. This colorimetric platform exhibited satisfactory LR (1–500 µM), low LOD (0.3 µM), and fortification recoveries (86.6–99.6%). In the chromogenic reaction catalyzed by Fe 3 S 4 @MN-CDs, ·O 2 − played a key role in concomitant with the participation of •OH and h + . Both the colorimetric assay and smartphone-based intelligent sensing show great promising in on-site monitoring of p -AP under field conditions. Graphical Abstract
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