INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Given Chisha,Chunlu Li,Lei Xiao,Bo Wang,Yueyuan Chen,Zhifang Cui
This study aimed at efficient rosmarinic acid (RA) extraction from rosemary ( Rosmarinus officinalis L.) using natural deep eutectic solvents (NADESs). Quantum chemical calculations and molecular dynamics simulation were used to dissect the microscopic mechanisms. Three choline chloride (ChCl) based NADESs and ethanol (traditional extractant as reference) were investigated. The σ -profiles of RA and each solvent were analyzed. The infinite dilution activity coefficient ( γ ∞ ) of RA in different solvents, the total non-bonded interaction energy and the average H-bond number were calculated, to predict the RA solubility, understand the bond type and strength in each system. Among the solvents studied, ChCl/LA (lactic acid) exhibited wider complementary σ -profile with that of RA, as well as lower ln γ ∞ , higher H-bond number and predominant electrostatic-interaction. Moreover, self-diffusion coefficients revealed that RA had better compatibility with ChCl/LA. Additionally, with different solvents as extractant, ChCl/LA outperformed the others by exhibiting the highest RA extraction yield. Based on results from single factor experiments, Box-Behnken design with response surface methodology was performed for further process optimization. The regressed model obtained was reliable from the ANOVA analysis of variances. RA extraction yield was 6.10% under optimal conditions of 69 °C, 5.6 h, liquid to solid ratio 34 and water content 13.5% (w/w). The combination of simulation and experiment in this study can shed light on the exploration of natural product extraction with high efficiency using green and novel NADESs.
研究领域:
化学工程
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药学
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分析化学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
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2025
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IF 8
Duan Zhou,Mouming Zhao,Xiaorong Zhong,Ning Liu,Xuefeng Chen,Daoming Li
1,3-diacylglycerol (DAG) is a natural lipid and known as functional oil. Here, we evaluated the potential of different natural deep eutectic solvents (NADESs) in synthesis of 1,3-DAG through Candida antarctica lipase B (CALB)-catalyzed esterification. Interestingly, we found that the 1,3-DAG content could be increased by 19.21 %, 13.10 %, and 8.74 % in three choline chloride (ChCl)-based NADESs, respectively, compared with that in solvent-free system. Confirmatory experiments demonstrated that NADESs could inhibit the acyl transfer of 1,3-DAG through modulating the water activity of the reaction system. Meanwhile, circular dichroism spectra and fluorescence spectroscopy results indicated that NADESs could not change the secondary structure of CALB, but could affect the tertiary conformation of CALB. Furthermore, molecular dynamics studies showed that the NADESs could induce a more compact tertiary conformation of CALB and enhance the sn -1,3 positional selectivity of CALB. Overall, the NADESs could induce a higher 1,3-DAG yield through inhibiting the acyl transfer of 1,3-DAG and enhancing the sn -1,3 positional selectivity of CALB. This study provides theoretical support for enzymatic production of structural lipids in NADESs.
研究领域:
生物化学
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酶工程
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2025
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IF 3.3
Wu Yi,Hu Hui,Yang Guangzhen,Chen Yifan,Zhong Xiaoyu,Li Shanshan,Pan Ming,Li Yuanbin,Zhang Jiaheng
Natural deep eutectic solvents (NaDESs) with ultrasound-assisted (UAE) method was used to improve the extraction efficiency of polysaccharide from black truffles, which can overcome the limitations of conventional methods. It demonstrated that the extraction efficiency of polysaccharide by six NaDESs with UAE were better than those of conventional solvents. Among the NaDESs, betaine-citric acid (BetCi) NaDES showed the best performance, achieving a remarkable extraction yield of 9.38%, outperforming water, ethanol, and 1,3-butanediol by 0.4, 11.5 and 11.0 times, respectively. Moreover, the polysaccharide yield was further optimized by response surface method, based on three influence factors (NaDES concentration, liquid-to-solid, and ultrasonic time). Scanning electron microscopy images showed that compared to the traditional extraction methods, the plate-like structure fractured into smaller pieces of black truffles treated by BetCi NaDES with UAE, indicating deeper interactions between NaDES and black truffles. Furthermore, the skin penetration as well as antioxidant and anti-wrinkle properties of the black truffle polysaccharide extract were evaluated, revealing promising bioactivity. Overall, this study represents a comprehensive exploration of novel extraction methods, highlighting efficient polysaccharide extraction and its potential applications.
研究领域:
食品科学
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化学工程
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药学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Xizhe Fu,Di Wang,Tarun Belwal,Jing Xie,Yanqun Xu,Li Li,Ligen Zou,Lixia Zhang,Zisheng Luo
A deep understanding upon the relation between anthocyanin extraction performance and physicochemical properties of natural deep eutectic solvents (NADESs) was obtained, and a high-efficiency NADES (Choline chloride-Oxalic acid (ChOa)) was selected and coupled with pulse-ultrasonication for anthocyanin extraction from blueberry pomace . Response surface methodology was adopted to analyzed the significant factors’ influence, interactive effects on extraction yield and optimize the extraction conditions. The theoretical optimal conditions with highest yield of total anthocyanins content (TAC) were 3.2 min of ultrasonic time, 325 W of ultrasonic power, 349.15 K of temperature, 60 mL/g of solvent to solid ratio, under which the experimental TAC value (24.27 ± 0.05 mg C3GE/g DW) was highly correlated with theoretical result (24.28 mg C3GE/g DW). Multi-stability protective effect of ChOa on anthocyanin over acidified ethanol was revealed by stability assays in high temperature, varying pH and light exposure conditions. FT-IR and DSC analysis further characterized the influence of ChOa over acidic ethanol on typical anthocyanins functional groups and thermal behavior of anthocyanin extracts. UPLC-Triple-TOF/MS analysis identified 10 anthocyanins that mainly consisted of delphinidin , petunidin , and malvidin compounds. Overall, this study provided a green, high-efficiency and multi-stability protective strategy for the recovery of anthocyanins from plant source by-products.
研究领域:
食品科学
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化学工程
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分析化学
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植物科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）