INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Min Zhang,Junhui Yang,Qili Yang,Liulian Huang,Hui Wu,Lihui Chen,Cuicui Ding
The poly(γ-glutamic acid)-NHS (γ-PGA-NHS) esters were used to endow collagen with both of excellent water-solubility and thermal stability via cross-linking reaction between γ-PGA-NHS and collagen. In the present work, the effect of γ-PGA-NHS on the aggregation of collagen molecules was studied by fluorescence techniques. The fluorescence emission spectra of pyrene in collagen solutions and the intrinsic fluorescence emission spectra of collagen suggested different effects of γ-PGA-NHS on collagen molecules: inhibiting aggregation below critical aggregation concentration (CAC) and promoting aggregation above CAC. The two-dimensional (2D) fluorescence correlation spectra indicated that the intermolecular hydrogen bonding and cross-linking between γ-PGA-NHS and collagen would influence the aggregation of collagen molecules. By the ultra-sensitive differential scanning calorimeter (VP-DSC), it was found that the main denaturational transition temperature ( T m2 ) of modified collagen increased, while its calorimetric enthalpy changes (Δ H 2 ) decreased compared to those of native collagen, further indicating that the modification of γ-PGA-NHS influenced the aggregation of collagen molecules. The study provide useful information for the utilizing and or the processing of water-soluble collagen in aqueous solution in the fields such as cosmetics, health care products , tissue engineering and biomedical materials , etc.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2026
·
IF 8.3
Yannan Xue,Chen Li,Yuan Ma,Yongxin Hu,Meiyue Ma,Yixuan Wu,Lei Zhao,Wenxuan Jiang,Jiayu Yang,Xiao Liu,Tiantian Sun,Qi Gao,Shuai Meng,Qiang Liu,Tiehai Li,Chengli Zong
Conventional microarray chemistries are generally optimized for amine-bearing probes, whereas native polysaccharides and glycolipids often require prior derivatization for immobilization, which risks altering key carbohydrate epitopes. Here, we developed a dual-anchor microarray platform ( DAMP ) by modifying commercially available poly-L-lysine-coated slides with NHS esters and diazirine photo-crosslinkers. The key innovation of this platform is the complementary dual-chemistry design on a high-loading 3D surface: NHS chemistry enables efficient immobilization of amine-bearing probes, whereas diazirine-mediated photo-crosslinking enables derivatization-free capture of underivatized native glycans. The resulting platform enabled rapid immobilization of native polysaccharides and glycolipids under mild UV irradiation and showed markedly enhanced sensitivity for amine-bearing glycans:10 pg/mL for a synthetic glycan and 0.32 ng/mL for purified pneumococcal cell wall polysaccharide. DAMP also supported the immobilization of 29 structurally distinct carbohydrates with retained lectin recognition, as well as direct analysis of 13 native capsular polysaccharides for pneumococcal serotyping and vaccine-response profiling. This work establishes a practical and sensitive surface platform for high-fidelity carbohydrate immobilization and expands the utility of microarrays in polysaccharide research and glycan-based vaccine studies.
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高影响因子
Materials Today Chemistry
·
2026
·
IF 7.4
Congyi Mao,Hock Eng Khoo,Di Huang,Xiuwen Li,Caiying Li,Xiaobo Chen,Fang Liu,Xia Li
Valorization of sugarcane bagasse xylan, an agricultural byproduct, for sustainable material design. • CXY–CS hydrogel fabricated via EDC/NHS-mediated covalent crosslinking between carboxymethyl xylan and chitosan. • The polysaccharide-based hydrogel effectively adsorbs three heavy metal ions: Cr(VI), Cu(II), and Zn(II). • A unique reduction-coordination mechanism is revealed for Cr(VI) removal, distinguishing it from typical cation adsorption. The development of high-performance adsorbents from agricultural waste is hindered by the limited solubility and the absence of functional groups in native polymers. To address these challenges, this study fabricated a novel hydrogel by crosslinking carboxymethylated xylan (CXY) with chitosan (CS) via an environmentally benign amidation reaction. A series of systematic batch adsorption experiments was performed at low concentrations (0–20 mg L −1 ) to investigate the adsorption kinetics and thermodynamics. A series of high-concentration tests (500 mg L −1 ) was conducted to ascertain the maximum adsorption capacities and cyclic regeneration performance for Cu 2+ , Zn 2+ , and Cr(VI). The underlying mechanism was elucidated through X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations. The adsorption process exhibited conformance with the pseudo-second-order kinetic model and Langmuir isotherm, thereby indicating monolayer chemisorption. The thermodynamic parameters confirmed the spontaneity and exothermic nature of the process. In conditions of high concentration (500 mg L −1 ), the hydrogel exhibited noteworthy maximum adsorption capacities (Q max ) of 129.23, 123.24, and 126.19 mg g −1 for Cu 2+ , Zn 2+ , and Cr(VI), respectively, demonstrating over 85% adsorption efficiency following five regeneration cycles. Mechanistic analysis revealed a synergistic “reduction-coordination” pathway, while the highly toxic Cr(VI) undergoes unique surface reduction to less hazardous Cr(III). The integration of experimental and theoretical approaches in this study provides a systematic clarification of the adsorption performance and the dual-function “reduction-coordination” mechanism of the hydrogel toward multiple heavy metals. The study provides a robust scientific foundation for the development of sustainable, waste-derived materials for the remediation of complex heavy metal pollution. Download: Download high-res image (317KB) Download: Download full-size image
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JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Shichen Zeng,Xu Liang,Mingang Zhao,Yanxiong Ren,Hanze Ma,Ziting Zhu,Yuhan Wang,Shaoyu Wang,Junyi Zhao,Guangzhaoyao Yang,Xuerui Wang,Fusheng Pan,Guangwei He,Zhongyi Jiang
Covalent organic frameworks (COF) are deemed as disruptive membrane materials owing to their versatile functionalities, high stability and ordered nanochannels. However, the size mismatch between the COF intrinsic pore and gas molecules becomes a grand challenge when using COF membrane for carbon capture. Herein, we propose a new type of polymer-functionalized COF laminar membranes through grafting CO 2 -phlic polyethyleneimine (PEI) polymer onto carboxylic acid COF (cCOF) laminar membranes. The cCOF nanosheets with high density of carboxyl groups are prepared via modified single phase solution method and then assembled to fabricate membranes. Further, the cCOF membranes are functionalized with PEI using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-Hydroxysuccinimide (EDC/NHS) coupling chemistry. The introduction of abundant amino groups from PEI afford the affinity of membranes toward CO 2 . Meanwhile, the graft of the PEI polymers reduces the defects or pinholes on the membrane surface. As a result, the PEI functionalized cCOF membranes exhibit a high CO 2 permeance of 1004 GPU and a CO 2 /N 2 selectivity of 33.7, which reach the carbon capture target performance and show great prospect for treating real flue gas. Our approach of functionalizing cCOF membranes with tailored functionalities may inspire the design of molecule-selective COF membranes.
研究领域:
材料科学
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环境科学
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高影响因子
Journal of Materials Chemistry B
·
2025
·Vol. 13
·期 7
·2469-2479
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IF 5.7
Chenyang Li,Yang Qian,Xueping Zhang,Rongwu Wang
Bioadhesive hydrogels show great promise in wound closure due to their minimally invasive nature and ease of use. However, they typically exhibit poor wet adhesion and mechanical properties on wet tissues. Herein, a ready-to-use bioadhesive hydrogel (denoted as PAA-NHS/C-CS) with rapidly robust adhesion and high mechanical strength is developed via a simple one-pot UV crosslinking polymerization of acrylic acid (AA), catechol-functionalized chitosan (C-CS), and acrylic acid N-hydroxysuccinimide ester (AA-NHS ester). Benefitting from the hydrogen bonds and electrostatic attractions formed between PAA-NHS and C-CS, the as-prepared hydrogel exhibits high tensile strength (∼630 kPa), fracture strain (∼1950%), and toughness (∼4250 kJ m−3) in the fully swollen state. Besides, the noncovalent interactions and covalent crosslinking formed between the dual adhesive moieties (the NHS ester and catechol groups) and the tissue surface endow the hydrogel with high shear strength (∼160 kPa), interfacial toughness (∼630 J m−2), and burst pressure (∼447 mmHg) on wet porcine skin. By integrating the high mechanical properties, rapid robust adhesion, and operational convenience, the as-prepared PAA-NHS/C-CS hydrogel shows great promise in wound closure.
研究领域:
生物材料学
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医学工程
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PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2017
·Vol. 41
·期 5
·e12395
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IF 1
Shuai Cui,Qin-Wei Zhou,Xiao-Ling Wang,Si-Qi Yang,Kai Chen,Zhi-Yuan Dai,Yao-Wen Huang,Tao Zhou
Bovine pancreatic lipase was immobilized onto N -succinyl-chitosan beads by using N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide (EDC)/ N -hydroxysuccinimide (NHS) as the activating agent. The immobilized lipase was characterized by Fourier transform infrared spectra and scanning electron microscopy. The activity of the immobilized lipase obtained under the optimized immobilization conditions was found to reach 1,480 U/g, with enzyme activity recovery of 74.5%. Compared to the free lipase, thermal stability of the immobilized lipase was greatly improved. The reusability study indicated that the immobilized lipase retained 76.6% of its initial activity after being recycled five times. A preliminary study indicated that the prepared immobilized lipase can effectively enrich the polyunsaturated fatty acid in fish oil (increasing from 45.1 to 67.7%), of which the combined docosahexaenoic acid and eicosapentaenoic acid contents increased from 16.3 to 27.1%. Practical applications To overcome the drawbacks of using free lipase, an effective method was developed by immobilization of lipase onto N -succinyl-chitosan beads using EDC/NHS as the activating agent. The immobilized lipase prepared by this method may find application in the enrichment of polyunsaturated fatty acids of fish oil.
研究领域:
生物化学
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食品科学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Shengyu Li,Lihong Gong,Xijin Wu,Xianli Liu,Ningning Bai,Yingxue Guo,Xia Liu,Hong Zhang,Huiying Fu,Qiyang Shou
Currently, protein-based hydrogels are widely applied in soft materials, tissue engineering and implantable scaffolds owing to their excellent biocompatibility, and degradability. However, most protein-based hydrogels are soft brittle. In this study, a ductile and mechanically enhanced bovine serum albumin (BSA) hydrogel is fabricated by soaking the a 1-(3-dimethylaminopropyl)-3ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) induced BSA hydrogel in (NH 4 ) 2 SO 4 solution. An EDC/NHS coupling reaction induce protein coupling reactions that cause the BSA skeleton to resemble architectural load-bearing walls, protecting the integrity of the hydrogel and preventing collapse. The effects of the BSA and (NH 4 ) 2 SO 4 concentrations on the hydrogel mechanics are evaluated, and the possible strengthening mechanism is discussed. Besides, the highly kosmotropic ions greatly enhance the hydrophobic interaction within BSA gels and dehydration effect and their mechanical properties were significantly enhanced. The various mechanical properties of hydrogels can be regulated over a large window by soaking hydrogels into various ions. And most of them can be washed away, maintaining high biocompatibility of the protein. Importantly, the protein hydrogels prepared by this strategy could also be modified as strain sensors. In a word, this work demonstrates a new, universal method to provide multi-functional, biocompatible, strength enhanced and regulable mechanical pure protein hydrogel, combining the Hofmeister effect with -NH 2 /-COOH association groups.
研究领域:
生物材料学
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组织工程学
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PubMed
高影响因子
Foods
·
2026
·Vol. 15
·期 12
·2117
·
IF 6
Jiarun Gao,Le Yu,Xiang Wan,Leilei Sun,Wenkui Song
Ready-to-eat sea cucumbers (RSC) cannot be preserved at room temperature due to autolysis, which is closely related to the instability of collagen resulting from the disruption of hydrogen bonds. To investigate the protective effect ofN-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) cross-linking against disruption of hydrogen bonds and its role in stabilizing RSC quality at room temperature, this study designed comparative experiments involving EDC/NHS cross-linking treatments with varying sequences of hydrogen bonds disruption. The results indicated that EDC/NHS positively affects the stabilization of the collagen structure in RSC. The various quality parameters of both groups of RSC that underwent cross-linking treatment before and after hydrogen bonds disruption were significantly better than those of the control group, which only experienced the breaking of hydrogen bonds. Notably, the Eb group, which underwent EDC/NHS cross-linking treatment prior to the disruption of the hydrogen bonds network, yielded even more favorable results. Preliminary analyses of textural properties and moisture content suggested that EDC/NHS helps delay the deterioration of RSC quality. The levels of soluble components and carbonyl groups indicated that prior cross-linking treatment is more effective in mitigating collagen degradation and oxidation. Differential scanning calorimetry revealed that the reduction in ΔH for the Eb group was only 2.4%. Furthermore, fluorescence spectroscopy, Fourier transform infrared spectroscopy, and circular dichroism spectroscopy, examined from the perspectives of secondary and tertiary structures respectively, indicated that the cross-linking mechanism of EDC/NHS involves the formation of a more robust network of amide bonds, thereby preventing the disruption of hydrogen bonds and enhancing collagen stability, enabling it to better resist the cleavage of hydrogen bonds due to urea. The scanning electron microscope and Van Gieson’s staining techniques offer a clearer illustration of this point from a microscopic perspective. Moreover, molecular docking simulations have indicated the cross-linking mechanism of EDC/NHS at the atomic level, thereby establishing a scientific foundation for the potential application and development of EDC/NHS in room-temperature storage technologies for RSC.
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高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
·
2025
·Vol. 80
·期 4
·718-730
·
IF 1.1
Jin Biao,Jin Zhiyin,Jin Dongri
(S)-2,5-Dioxypyrrolidine-1-yl-(5-(4-methylpiperazin-1-yl)-2,4-dinitrophenyl) pyrrolidine-2-carboxylate ( PPZ-Pro-NHS ) was used as the reagent for chiral derivatization to distinguish between amino acid enantiomers. PPZ-Pro-NHS, synthesized from (S)-1-(5-(4-methylpiperazin-1-yl)-2,4-dinitrophenyl) pyrrolidine-2-carboxylic acid, reacted with chiral amines under mild conditions to yield the corresponding organic derivatives. The optimal conditions for derivatization using PPZ-Pro-NHS were 25°C and 40 min in a medium containing triethylamine. A simple liquid chromatography–mass spectrometry ( LC − MS ) method was developed to separate chiral amino acids using reversed-phase chromatography. The amino acids were separated by isocratic or gradient elution on a Mightysil RP-18 GP column with a mobile phase containing H 2 O, methanol, and 0.1% (v/v) formic acid. The enantiomers of the amino acids were fully separated with high resolution ( Rs = 1.20–5.30) and high sensitivity (limit of detection: 85–4546 fmol) using PPZ-Pro-NHS derivatization and LC–MS analysis with both isocratic and gradient elution. The enantiomers of 16 amino acid mixtures were simultaneously separated in a single chromatographic run using a gradient mobile phase system. Thus, PPz-Pro-NHS can be used for chiral amine screening and authentication.
研究领域:
分析化学
·
生物化学
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Jiarun Gao,Le Yu,Xiang Wan,Yuxue Shan,Jingfang Zhang,Mingbo Li,Leilei Sun
Ready-to-eat sea cucumbers (RSC) cannot be preserved at room temperature due to severe autolysis. This study systematically examined the quality-preserving effects of tea polyphenol (TP) and N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS), two cross-linking treatments, on RSC during storage at room temperature. The results demonstrated that both cross-linking treatments significantly delayed the deterioration of quality and exhibited antimicrobial effects, accompanied by enhanced the structural and thermal stability of collagen in RSC. Microstructural analysis further confirmed that TP and EDC/NHS strengthened the collagen fibers. Molecular docking simulations suggested that TP interacts with collagen primarily through non-covalent binding, whereas EDC/NHS achieves positional localization via electrostatic attraction, subsequently reinforcing the collagen structure through covalent amide bonds. Both strategies effectively slowed the deterioration of RSC during storage at room temperature, thereby providing a theoretical foundation for the development of advanced preservation technologies for RSC.
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POLYMER
·
2024
·
IF 4.1
Xiang Dong,Zhiyu Sun,De-long Li,Qiancheng Duan,Yan Ma,Song Liu
As epoxy resin (EP) is widely used in the microelectronic field, it is essential to improve its electromagnetic wave (EMW) absorption and flame retardancy simultaneously. In this work, we synthesized a novel nanohybrid, RMN (Fe 3 O 4 /NHS@M(OH)(OCH 3 )@rGO (M = Co, Ni)), with petal-shaped anisotropic interfaces, by using graphene oxide as the core and the 2D nanosheets M(OH)(OCH 3 ) (M = Co, Ni) as the petal, with nickel hydroxystannate and ferrite as further modifications. The RMN combines carbon-based materials, ferrites and conductors, and represents the petal-like structure with rich nondirectional interfaces. The results show that EP composite containing 25 wt% RMN represented excellent EMW absorption, with a minimum reflection loss of −55.79 dB (with 7.4 mm thickness at 3.1 GHz). Moreover, the EP composite with 15 wt% RMN (EP/RMN700-3) had the lowest peak heat release rate of 470.9 kW m −2 , which was 69.7 % lower than pure EP, showing a significantly improving in flame retardant. The thermal conductivity efficiency of EP/RMN700-3 increased to 128.4 % compared with the pure EP, which effectively reduces fire risk caused by heat accumulation. This study provides a valuable solution to overcome the challenges of EMW absorption and flame retardant for EP in the microelectronic materials field.
研究领域:
材料科学
·
电子工程
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高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Yi Zhang,Jinjun Dai,Ruiyue Hang,Xiaohong Yao,Long Bai,Di Huang,Ruiqiang Hang
Despite the clinical prevalence of various bone defect repair materials, a full understanding of their influence on bone repair and regeneration remains elusive. This study focuses on poly(acrylamide) (PAAm) hydrogels, popular 2D model substrates, which have regulable mechanical properties within physiological. However, their bio-inert nature requires surface biofunctionalization to enhance cell-material interactions and facilitate the study of bone repair mechanisms. We utilized PAAm hydrogels of varying stiffness (18, 76 and 295 kPa), employed sulfosuccinimidyl-6-(4′-azido-2′-nitropheny-lamino) hexanoate (sulfo-SANPAH) and N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride/N-hydroxysuccinimidyl acrylate (EDC/NHS) as crosslinkers, and cultured macrophages, endothelial cells, and bone mesenchymal stem cells on these hydrogels. Our findings indicated that sulfo-SANPAH's crosslinking efficiency surpassed that of EDC/NHS, irrespective of pore size and stiffness. Importantly, we observed that the stiffness and surface biofunctionalization method of hydrogels significantly impacted cell adhesion and proliferation. The collagen-modified hydrogels by EDC/NHS strategy failed to support the normal biological behavior of bone mesenchymal stem cells and hindered endothelial cell spreading. In contrast, these modified hydrogels by the sulfo-SANPAH method showed good cytocompatibility with the three types of cells. This study underscores the critical role of appropriate conjugation strategies for PAAm hydrogels, providing valuable insights for hydrogel surface modification in bone repair and regeneration research.
研究领域:
生物材料
·
组织工程
·
细胞生物学
·
生物医学工程
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PubMed
高影响因子
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