SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Qing Zhang,Shangshang Ma,Li Zhang,Hongyan Wang,Xin Zhuo,Guizhi Wang,Yanyun Shen,Keying Zhang,Shao Su
Microfluidic chip for sensing heavy metal ions has been strongly attended due to its merits, but still remains a large challenge from long analysis time derived from the preconcentration process of metal ions. Herein, a heated flow channel was manufactured by 3D printing combined with a filter paper to construct a microfluidic chip, the thermocapillary convection strategy was introduced to accelerate ions migration, allowing for rapidly recognizing metal ions. The MnO 2 nanosheets-Au nanostars (MnO 2 NSs-AuNSs) film was employed as modifier to fabricate a flexible screen printed electrode (SPE) for high-efficiency detecting metal ions based on thermocapillary convection accelerating accumulation method. To verify the proposed strategy, the detection of Cu 2+ was chosen as a model. The results revealed that the proposed strategy possessed a rapid detection to Cu 2+ , which greatly improved the detection efficiency (saved 400 s of preconcentration time) and achieved a detection limit down to 0.0270 ppb. Interestingly, the constructed method was successfully used to evaluate Cu 2+ concentration in sweat samples with satisfactory results. This proposed method maybe provide new insight to develop a novel approach to rapidly and sensitively detect metal ions, which possess huge utilization potentiality for safeguarding human health.
研究领域:
分析化学
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环境科学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
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IF 17.1
Xiaodi Zhang,Qi Li,Longfei Li,Jiang Ouyang,Tong Wang,Junjie Chen,Xiaoqing Hu,Yingfang Ao,Duotian Qin,Liqun Zhang,Jiajia Xue,Jin Cheng,Wei Tao
Bone fractures are often companied with poor bone healing and high rates of infection. Early recruitment of mesenchymal stem cells (MSCs) is critical for initiating efficient bone repair, and mild thermal stimulation can accelerate the recovery of chronic diseases. Here, a bioinspired, staged photothermal effect-reinforced multifunctional scaffold was fabricated for bone repair. Uniaxially aligned electrospun polycaprolactone nanofibers were doped with black phosphorus nanosheets (BP NSs) to endow the scaffold with excellent near-infrared (NIR) responsive capability. Apt19S was then decorated on the surface of the scaffold to selectively recruit MSCs toward the injured site. Afterward, microparticles of phase change materials loaded with antibacterial drugs were also deposited on the surface of the scaffold, which could undergo a solid-to-liquid phase transition above 39 °C, triggering the release of payload to eliminate bacteria and prevent infection. Under NIR irradiation, photothermal-mediated up-regulation of heat shock proteins and accelerated biodegradation of BP NSs could promote the osteogenic differentiation of MSCs and biomineralization. Overall, this strategy shows the ability of bacteria elimination, MSCs recruitment, and bone regeneration promotion with the assistance of photothermal effect in vitro and in vivo, which emphasizes the design of a bioinspired scaffold and its potential for a mild photothermal effect in bone tissue engineering.
研究领域:
组织工程
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生物材料
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生物医学工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2021
·Vol. 188
·期 12
·1-11
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IF 5.833
Wang Xiao,Liao Xiaochen,Zhang Bingjian,Zhang Mengmeng,Mei Lisha,Wang Fangwai,Chen Siyu,Qiao Xiuwen,Hong Chenglin
Abstract Carcinoembryonic antigen levels in the human body reflect the conditions associated with a variety of tumors and can be used for the identification, development, monitoring, and prognosis of lung cancer, colorectal cancer, and breast cancer. In this study, an amperometric immunosensor with CuCo/carbon nanocubes (CuCo/CNC) as the signal label is constructed. The bimetal-doped carbon skeleton structure has a high specific surface area and exhibits good electrocatalytic activity. In addition, Au/g-C 3 N 4 nanosheets (Au/g-C 3 N 4 NSs) are used to modify the substrate platform, facilitating the loading of more capture antibodies. The reaction mechanism was explored through electrochemical methods, X-ray powder diffraction, X-ray photoelectron spectroscopy, and other methods. Kinetic studies have shown that CuCo/CNC have good peroxidase-like activity. In addition, the electrocatalytic reduction ability of CuCo/CNC on hydrogen peroxide can be monitored using amperometric i – t curve (− 0.2 V, vs. SCE), and the response current value is positively correlated with the CEA antigen concentration. The prepared electrochemical immunosensor has good selectivity, precision, and stability. The dynamic range of the sensor was 0.0001–80 ng/mL, and the detection limit was 0.031 pg/mL. In addition, the recovery and relative standard deviation in real serum samples were 97.7–103 % and 3.25–4.13 %, respectively. The results show that the sensor has good analytical capabilities and can provide a new method for the clinical monitoring of CEA. Graphic abstract
研究领域:
生物医学工程
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肿瘤学
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分析化学
✓ 已验证
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2019
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IF 4.474
Shuan Zhang,Chunyan Zhang,Yilei Jia,Xiaobo Zhang,Yunhui Dong,Xinjin Li,Qing Liu,Yueyun Li,Zengdian Zhao
A sandwich-type electrochemical immunosensor was fabricated for the quantitation of alpha fetoprotein (AFP). To this end, the Au@Pt dendritic nanorods loaded with amino functionalized molybdenum selenide nanosheets (Au@Pt 41 DNRs/NH2-MoSe2 NSs) with enhanced peroxidase-like properties were selected as the secondary antibody label (Ab2) to achieve signal amplification. The as-obtained Au@Pt DNRs/NH2-MoSe2 NSs exhibited better catalytic activity toward hydrogen peroxide reduction and offered rich anchors for bioconjugation . Meanwhile, gold nanoparticles anchored on an amino functionalized graphene (Au NPs/NH2-GS) composite with admirable conductivity and biocompatibility was used as the matrix material to improve sensitivity. Under optimal conditions, amperometric current responses had a good linear relationship with the logarithm values of AFP concentration in the range 10 fg mL –1 to 200 ng mL –1 with a detection limit of 3.3 fg mL –1 (S/N = 3). Additionally, the immunosensor had excellent reproducibility, selectivity, and stability, which indicated superior performance for AFP detection compared with previous reports. The satisfactory results of human serum samples analysis showed that the designed immunosensor has potential applicability for the sensitive detection of other tumor markers.
研究领域:
分析化学
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生物化学
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纳米技术
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医学诊断
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Danyan Wang,Xiaojun He,Qiang Wang,Pingping Li,Enoch Obeng,Dongyang Zheng,Jianliang Shen,Zi Jin
Methicillin-resistant Staphylococcus aureus (MRSA) has been associated with significant mortality and longer hospital stays. Artificial nanozymes not requiring antibiotics can generate enough reactive oxygen species (ROS) to kill bacteria. In this study, we synthesized hexagonal copper sulfide nanosheets (CuS NSs) using a modified one-pot synthesis. The CuS NSs demonstrated photothermal stability and superior photothermal conversion efficiency due to large surface area and existence in the second near-infrared (NIR-II) window (1270 nm), promoting the production of ROS. Upon NIR-II irradiation, CuS NSs displayed an excellent biocatalytic antibacterial role in vitro and accelerated abscess resolution, and enhanced the recovery of MRSA-infected wounds in vivo . Moreover, CuS NSs demonstrated negligible toxicity following an amplifying peroxidase-like activity through hyperthermia. Overall, CuS NSs provide a feasible strategy for using the synergistic therapeutic platform for effective therapy of deep-seated MRSA-infected wounds.
研究领域:
纳米技术
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生物医学工程
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传染病学
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材料科学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2021
·Vol. 31
·期 20
·2101372
·
IF 18.808
Chen Yang,Zhiwei Zheng,Muhammad Rizwan Younis,Chenle Dong,Yahong Chen,Shan Lei,Dong-Yang Zhang,Jiayingzi Wu,Xueqing Wu,Jing Lin,Xiansong Wang,Peng Huang
Patients with diabetes mellitus (DM) suffer from a high risk of fractures and poor bone healing ability. Surprisingly, no effective therapy is available to treat diabetic bone defect in clinic. Here, a 3D printed enzyme-functionalized scaffold with multiple bioactivities including osteogenesis, angiogenesis, and anti-inflammation in diabetic conditions is proposed. The as-prepared multifunctional scaffold is constituted with alginate, glucose oxidase (GOx), and catalase-assisted biomineralized calcium phosphate nanosheets (CaP@CAT NSs). The GOx inside scaffolds can alleviate the hyperglycemia environment by catalyzing glucose and oxygen into gluconic acid and hydrogen peroxide (H 2 O 2 ). Both the generated H 2 O 2 as well as the overproduced H 2 O 2 in DM can be scavenged by CaP@CAT NSs, while the initiated hypoxic microenvironment stimulates neovascularization. Moreover, the incorporation of CaP@CAT NSs not only enhance the mechanical property of the scaffolds, but also facilitate bone regeneration by the degraded Ca 2+ and PO 4 3− ions. The remarkable in vitro and in vivo outcomes demonstrate that enzymes functionalized scaffolds can be an effective strategy for enhancing bone tissue regeneration in diabetic conditions, underpinning the potential of multifunctional scaffolds for diabetic bone regeneration.
研究领域:
生物材料
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组织工程
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糖尿病研究
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Yanzhen Sun,Xiaodong Jing,Yang Liu,Bing Yu,Hao Hu,Hailin Cong,Youqing Shen
Nucleic acid-based agents have advantages in therapeutic efficacy and biological safety. However, due to its facile degradability, it lacks an effective route of administration in wound treatment. Designing smart hydrogels for the spatiotemporally controllable delivery of nucleic acids is of great significance for clinical applications. Here, a near-infrared (NIR)-responsive nanocomposite hydrogel was prepared using methyl methacrylate (GMA)-modified chitosan as the macromolecular cross-linker, N-isopropylacrylamide (NIPAAm) as the backbone, and molybdenum disulfide nanosheets (MoS 2 NSs) as the nanocomponents. The polydeoxyribonucleotide (PDRN), a nucleic acid-based agent that promotes tissue regeneration , was loaded and delivered. The photothermal conversion capability of MoS 2 NSs enables customized care of PDRNs and antibacterial enhancement. In a full-thickness skin defect model, high-quality wound healing effects were demonstrated under the action of nanocomposite hydrogels. The proposed nanocomposite hydrogel provides a new reference for local delivery of nucleic acid-based agents.
研究领域:
材料科学
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生物医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Huiyu Zou,Wuming Wu,Jingjing Zhou,Chunyan Deng
In this study, a novel integrated photoelectrochemical (PEC) sensor platform was proposed, utilizing an optical fiber (OF) as the working electrode for guided in situ light. A CdS quantum dots (QDs)/ZnO nanosheets (NSs) n-n heterojunction was quickly and easily constructed on the OF surface by successive ionic layer adsorption and reaction (SILAR). Au nanoparticles (NPs)@dsDNA as a capturing probe were modified on the CdS QDs/ZnO NSs@OF (CZ@OF). Due to the energy transfer between Au NPs@dsDNA and CdS QDs, the resultant opto-electrode has a lower background near zero, enabling the “signal-on” detection of biomarkers (interleukin-6 (IL-6) as a model). The OF-PEC biosensor demonstrated a wide linear range from 1 to 100 pg mL–1 with a regression coefficient (R2) of 0.9958 and an impressive detection limit (LOD) of 0.19 pg mL–1. More significantly, the proposed OF-PEC can be successfully used for the detection of IL-6 in serum samples from patients with pulmonary arterial hypertension, and it showed consistency and is more sensitive to trace concentrations compared to BD FACSCanto II flow cytometry used at the hospital. This holds significance for an early disease diagnosis. Therefore, the proposed OF-PEC not only achieves integration of the light source and sensing interface but also enables sensitive and accurate “signal-on” detection of IL-6. Furthermore, due to the flexibility and remote detection capabilities of OF, the application of OF-PEC is expected to be expanded more widely. This approach opens up possibilities for advances in PEC sensing.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）