RUSSIAN JOURNAL OF APPLIED CHEMISTRY
·
2016
·Vol. 89
·期 7
·1145-1157
·
IF 0.307
Yu Ting,Yang Xu,Yuan Jianlan,Guo Li,Wu Xiaoling,Wang Hao
In drilling and production process, the barite powder serving as weighting agent is added to ensure operation safety when overflow occurs, that results in reservoir pollution and pore blockage. The main component of barite is BaSO 4 , so ammonium carboxy chelating agents can be injected into formation to form stable chelates with Ba 2+ , BaSO 4 will be eliminated from wellbore with flowback fluid easily. In this experiment, the weight method and the atomic absorption spectrometry method were utilized to study descaling ability and an action mechanism of descaling agents. Firstly, DTPA was selected from three descaling agents: NTA, EDTA, DTPA; then OT1 was screened out to synergize with DTPA and studied its descaling mechanism; eventually, the descaling system STD was determined, and its composition was 6% DTPA + 5% OT1 + 1% antiswelling agent + 100 ppm corrosion inhibitor. And the STD’s performance evaluation experiment results suggested that it had an excellent resistance to temperature and demonstrated a good descaling ability at 170°C. Furthermore, the descaling ability of STD was almost not affected by calcium and magnesium ions and its corrsion rate is lower than standard value. Moreover, STD could descale efficiently at lower dosage. So STD can be widely applied to descale in oilfield.
研究领域:
石油工程
·
化学工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Ningyi Chen,Bingcai Pan
Toxic metal-organic complexes are ubiquitous in contaminated waters but more challenging than free ion counterparts for water decontamination. Currently, almost all the available analytical methods, including Au nanoparticles-mediated colorimetric assay, cannot discriminate metal-organic complexes from the total metal content, which greatly impedes the development of specific decontamination strategies for metal-organic complexes. In this study, we found that nitrilotriacetic acid (NTA) functionalized AuNPs could be used to determine various Cr(III) complexes (Cr(III)-lactate, Cr(III)-malonate, Cr(III)-oxalate, Cr(III)-tartrate, Cr(III)-citrate and Cr(III)-EDTA) in water. Mechanism study indicated that at acidic pHs (pH = 1.6), the protonation of organic ligands resulted in partial dissociation of Cr(III) from the corresponding complexes to expose its accessible orbitals for re-coordination with NTA. Such re-coordination induces the aggregation of the dispersed NTA-AuNPs and thereafter causes considerable changes both in color and UV–Vis spectrum of the solution. The nanosensor is highly selective to Cr(III)-organic complexes with detection limits of 0.029 μM for Cr(III)-lactate, 0.043 μM for Cr(III)-malonate, 0.35 μM for Cr(III)-oxalate, 0.079 μM for Cr(III)-tartrate, 2.58 μM for Cr(III)-citrate and 0.90 μM for Cr(III)-EDTA, respectively. The feasibility of the proposed colorimetric assay has been verified by using the authentic natural and industrial water samples. We believe this study will be of fundamental significance to push forward the application of Au NPs colorimetric assay in authentic waters contaminated by toxic metals.
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2018
·Vol. 10
·期 3
·435-439
·
IF 2.917
Xu Hongpan,Sun Tingting,Huang Yuanfu,Song Lian,Li Zhiyang,Wang Wei
Placing of affinity tags at desired position of the proteins has been widely used to facilitate protein separation. One of the most commonly method for protein separation is nickel ion (Ni2+) affinity chromatography. Complex steps and time consuming in nickel ion affinity
chromatography have caused high demand for alternative purification methods that allow direct and rapid separation of His-tagged proteins. In the present study, magnetic core–shell beads enriched with Ni2+-nitrilotriacetate (Ni-NTA) on their surface were prepared in this study
by precipitation polymerization, with a uniform size of about 10 μm. The presented Ni2+ amount was 2.23%, which endowed the spheres with excellent magnetic responsivity and dispersity. We compared the magnetic beads enrichment method and nickel ion affinity chromatography
method for extraction of His-tagged protein sample with different concentrations. The results revealed that, when compared with nickel ion affinity chromatography, extraction efficiency, purity of the extracted protein, and regeneration effort were much higher with the magnetic beads enrichment
method. By using magnetic beads enrichment method, samples could be separated within 20 s, and purified within 1 h, with unlimited flow velocity and sample capacity. In addition, the magnetic beads enrichment method was particularly promising for the separation of His-tagged proteins in less
samples.
研究领域:
生物化学
·
蛋白质组学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Yu Chen,Yajing Li,Yongjian Wang,Igor Ying Zhang,Rongfu Huang
This work investigated the activation of peroxymonosulfate by electrochemical (EC) system assisted with Fe(III)-nitrilotriacetic acid (NTA) complex for degradation of persistent naphthenic acids (NAs) under neutral initial pH conditions. As NAs are a complicated mixture, 1-adamantanecarboxylic acid (ACA) was selected as the model NA compound for degradation experiment. The addition of NTA is to chelate with Fe(III), gaining stability under neutral pH condition to facilitate the circulation of Fe(II)/Fe(III) by the electrochemical process to activate PMS. The EC/Fe(III)-NTA/PMS system was explored with applicable pH range of 3–9 and an optimized molar ratio 1: 2 for Fe: NTA. Results of quenching and chemical probe experiment together with results of electron paramagnetic resonance (EPR) analysis revealed the main reactive species of the system, including • OH, SO 4 •− , 1 O 2 and possibly Fe(IV). With the addition of NTA, the yields of • OH, SO 4 •− , 1 O 2 were enhanced. Results of mass spectrometry analysis and DFT calculations indicated the formation of 9 degradation byproducts of ACA via three primary degradation pathways such as hydroxyl substitution, carbonyl substitution, and decarboxylation . Furthermore, the EC/Fe(III)-NTA/PMS system could achieve excellent removal efficiency of ACA with different anions such as Cl - , HCO 3 - , NO 3 - and H 2 PO 4 - in the background. The practical applicability of the system was also verified with the high removal of commercial NAs mixture standard. Overall results have indicated the EC/Fe(III)-NTA/PMS system could be utilized for efficient reclamation of authentic oil and gas industrial wastewater under natural pH conditions.
研究领域:
环境科学
·
化学工程
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Chenghan Ji,Yuanjie Zhang,Zhuoyao Fang,Yi Ren,Weiming Zhang
Current research on heavy metal adsorbents primarily focuses on eliminating particular heavy metal ions. Nevertheless, there remains a pressing demand for adsorbents capable of efficiently capturing a wide range of heavy metals found in actual wastewater. We report a metal–organic framework (MOF-808-NTA) functionalized with aminotriacetic acid (NTA), which has broad-spectrum capabilities for capturing heavy metal ions. Experiments with 15 distinct heavy metal ions, including hard (e.g., Cr 3+ ), soft (e.g., Hg 2+ ), and borderline Lewis metal ions (e.g., Pb 2+ ), found that the material achieved over 99 % removal efficiency in both individual ion and single-ion scenarios. Importantly, MOF-808-NTA exhibited ultrafast adsorption kinetics (removal >99 % within 30 s) for multiple metal ions, including Cr 3+ , Hg 2+ , and Pb 2+ , excellent selectivity (removal >95 %) when competing substances like Na + , Ca 2+ , and humic acid; and remarkable recyclability (removal >97.5 % after 5 cycles). Furthermore, spectrum analysis and theoretical calculation calculations indicate that the NTA groups in MOF-808-NTA serve as the primary sites for adsorption. The nitrogen and two oxygen atoms within the NTA groups create claw-like structures, which ensure exceptional capture of heavy metal ions. This work underscores the promise of MOF-808-NTA to function as a versatile material for capturing diverse heavy metals from wastewater.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Weiyang Dong,Yaoyao Jin,Kang Zhou,Sheng-Peng Sun,Yifan Li,Xiao Dong Chen
Ferric-nitrilotriacetate complex (Fe III -NTA) has been adopted to catalyze the photo-Fenton degradation of emerging pharmaceutical micropollutants in water and wastewater at neutral pH. The generation of hydroxyl radicals (HO ) in UVA/Fe III -NTA/H 2 O 2 was identified by using electron spin resonance (ESR) trapping technique. The effects of critical parameters (e.g., NTA:Fe III molar ratio, Fe III -NTA and H 2 O 2 dosages) on the steady-state HO concentrations were studied in terms of the degradation of carbamazepine (CBZ, as a model compound) in Milli-Q water. In addition, the degradation of pharmaceuticals mixtures (including CBZ, crotamiton (CRMT) and ibuprofen (IBP)) in wastewater effluents from a biological aerated filter (BAF) by UVA/Fe III -NTA/H 2 O 2 was studied in continuous-flow mode. The results showed that the efficacies of Fe III -NTA in catalyzing photo-Fenton degradation of pharmaceuticals in wastewater effluents were comparable to those obtained by Fe III -ethylenediamine-N,N′-disuccinic acid (Fe III -EDDS), and far exceeded other Fe III -L complex (e.g., citric acid , malonic acid, oxalic acid and tartaric acid). More than 92% degradation efficiencies of CBZ, CRMT and IBP were obtained in continuous-flow mode under the given conditions of 0.178 mM Fe III -NTA (1:1), 4.54 mM H 2 O 2 , UVA intensity 4.05 mW cm −2 , hydraulic retention time (HRT) 2 h, influent pH 7.6 (±0.2) and temperature 20 °C. The results presented herein suggest that Fe III -NTA-catalyzed neutral photo-Fenton reaction can be an alternative tertiary process for the treatment of pharmaceutical micropollutants in secondary wastewater effluents.
研究领域:
环境科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Zilong Zhang,Jiahuan Chen,Lixiang Chen,Kaihua Long,Lejing Qu,Silin Huang,Xinyi Yuan,Xu Ji,Qian Li,Xinfeng Zhao
Functional protein immobilization forms the basis for bio-detections. A series of one-point, site-specific immobilization methods have been developed, however, it still remains as a challenge how to avoid the proteins to move in all directions as well as conveniently regenerate the bio-devices. Herein, we have developed a bivalent affinity binding-inspired method for PPARγ immobilization using DNA aptamer and nickel-nitrilotriacetic acid (Ni 2+ -NTA) chelation. The specific DNA aptamer ( Apt 2 ) was selected by an on-column systematic evolution of ligands by exponential enrichment (SELEX) method with affinity of (1.57 ± 0.15) × 10 5 M −1 , determined by isothermal titration calorimetry (ITC). Apt 2 and nickel-nitrilotriacetic acid (Ni 2+ -NTA) were modified on macroporous silica gels via L-α-allylglycine as a linker. They respectively interacted with PPARγ and 6×His tag via bivalent affinity binding for the receptor immobilization. After comprehensive surface characterization, PPARγ was proved to be successful immobilized. Chromatographic studies revealed that the immobilized PPARγ has conformation selectivity, which discriminated agonist and antagonist of the receptor. Ligand-binding parameters (affinity and rate constant) of four agonists (rosiglitazone, pioglitazone, troglitazone, and magnolol) with PPARγ were determined. Troglitazone showed the lowest dissociation rate constant. The binding affinities (3.28 × 10 7 , 1.91 × 10 6 , 2.25 × 10 7 , and 2.43 × 10 7 M −1 ) were highly consistent with the data obtained using purified receptor in solution (2.16 × 10 7 , 4.52 × 10 6 , 1.20 × 10 7 , and 1.56 × 10 7 M −1 ), offering reliable bio-detection method for PPARγ and its ligands. Due to the biocompatibility of nuclear receptor with DNA, it is conceivable that the bivalent affinity-based method will be a general method for the immobilization of other nuclear receptors, which may provide selective conformation and improved ligand-binding activity for the receptors.
研究领域:
生物化学
·
分子生物学
·
生物技术
·
分析化学
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Cuilin Meng,Qiongfang Zhuo,Anqi Wang,Jingjing Liu,Zehong Yang,Junfeng Niu
In this study, TiO 2 nanotube arrays (TiO 2 -NTA) were used as an intermediate layer to provide effective electrocatalytic activity and stability for Ti/TiO 2 -NTA/Ti 4 O 7 anode. Compared with Ti/Ti 4 O 7 anode, Ti/TiO 2 -NTA/Ti 4 O 7 anode exhibited higher oxygen evolution potential (2.40 V), larger active specific surface areas (1.81 m 2 g −1 ), stronger radical generation capacity (64.42 μM), longer accelerated service life (56.0 h), and superior favipiravir removal ratio. The flow-through electrochemical reaction system was constructed by using the porous Ti/TiO 2 -NTA/Ti 4 O 7 anode. The removal ratio, TOC removal ratio and mineralization current efficiency of favipiravir in the flow-by electrochemical system were significantly improved and the energy consumption was reduced compared with the conventional flow-by electrochemical system, verifying the superiority of the porous flow-through Ti/TiO 2 -NTA/Ti 4 O 7 anode. The effects of operating parameters on the removal of favipiravir in the flow-through electrochemical reaction system were investigated. The degradation mechanisms of favipiravir are the synergetic effects of the free radical (·OH and SO 4 − ·) and the direct electron transfer. Ti/TiO 2 -NTA/Ti 4 O 7 anode displayed excellent stability in five consecutive cycles, exhibited significant removal ratio (87.7%) of favipiravir from actual wastewater, and remained efficient and versatile for a wide range of typical PPCPs pollutants. Therefore, Ti/TiO 2 -NTA/Ti 4 O 7 is a promising porous anode material in the engineering application.
研究领域:
环境科学
·
电化学工程
·
药物处理
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DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2026
·
IF 3.8
Hongjian Chen,Huanjiang Niu,Yunpeng Wang,Jiannan Jia,Songle Wang,Yuwen Yuan,Pragati Awasthi,Xvsheng Qiao,Shisheng Ling,Zhenzhen Ge,Dong Chen,Wenkun Dong,Guodong Qian,Xianping Fan,Yubing Dong
Lanthanide complexes based fluorescent microspheres are widely used in lateral flow immunoassay (LFIA) measurements of small melecules, such as methamphetamine (MET), due to their efficient fluorescence. However, the easily occurred aggregation of lanthanide complexes often leads to concentration quenching effect of fluorescence, which severely deteriorate the biodetection performance of fluorescent microspheres. Here, in order to optimize the distribution of lanthanide complexes, the crosslinked agent dimethacrylate (EGDMA) is introduced in the microspheres polymerization. By adjusting the concentration of EGDMA, the aggregation of lanthanide complexes is reduced, which inhibits fluorescence quenching effect and enhances fluorescence intensity of the fluorescent microspheres. Such fluorescent microspheres thus achieve highly sensitive detection of MET, with showing a detection limit of 0.048 ng mL −1 with excellent stability. This study provides a new idea for the optimal design of lanthanide complexes dispersed fluorescent latex beads, which is expected to promote the development of advanced In Vitro Diagnostics (IVD) technologies to rapid and precise screening of small molecules.
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高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Liya Zhou,Yaping Ouyang,Weixi Kong,Teng Ma,Hao Zhao,Yanjun Jiang,Jing Gao,Li Ma
In this study, a novel kind of Ni-NTA modified monodispersed SiO 2 nanoflowers (Ni-NTA@SiO 2 nanoflowers) were successfully synthesized. The obtained Ni-NTA@SiO 2 nanoflowers were used to specifically adsorb and purify His-tagged old yellow enzyme (OYE1) and glucose dehydrogenase (GDH), which allows access to optically pure (3 S)− 3-methyl-cyclohexanone through asymmetric hydrogenation reaction, and forms a cofactor regeneration system. The protein loading amount on Ni-NTA@SiO 2 nanoflowers was 40.17 mg/g support and the activity recoveries of OYE1 and GDH were 81.53% and 79.68%, respectively. The effects of pH and temperature on the activity of free and co-immobilized enzymes were investigated, and the stability as well as reusability were also measured. Compared to free enzymes, the co-immobilized enzymes showed higher thermal and storage stability. The co-immobilized enzymes were applied to asymmetric reduction of C C bonds for the synthesis of a chiral center with excellent enantioselectivity (ee > 99%), and the conversion was 46.02% after 7 cycles. This work introduced a one-pot multi-enzyme purification and co-immobilization strategy to construct efficient cofactor regeneration system with high activity and stability.
研究领域:
生物化学
·
酶工程
·
纳米材料科学
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Shao Fei,Yu Lei,Zhang Yang,An Chuanfeng,Zhang Haoyue,Zhang Yujie,Xiong Yi,Wang Huanan
Microfluidics-based alginate microgels have shown great potential to encapsulate cells in a high-throughput and controllable manner. However, cell viability and biological functions are substantially compromised due to the harsh conditions for gelation, which remains a major challenge for cell encapsulation. Herein, we presented an efficient and biocompatible method by on-chip triggered gelation to generate microfluidic alginate microgels for single-cell encapsulation. Two calcium complexes of calcium–ethylenediaminetetraacetic acid (Ca-EDTA) and calcium–nitrilotriacetic (Ca-NTA) as crosslinkers for triggered gelation of alginate were compared and investigated for feasible application. By triggered release of Ca2+ ions from the calcium complex via adding acetic acid in the oil phase, the alginate precursor in the aqueous droplets can be crosslinked to form alginate microgels. Although using Ca-EDTA and Ca-NTA both achieved on-chip gelation, Ca-NTA led to significantly higher cell viability since the dissociation of Ca2+ ions from Ca-NTA can be obtained using less concentration of acid compared to Ca-EDTA. We further demonstrated the functionality of encapsulated mesenchymal stem cells (MSCs) in alginate microgels prepared using Ca-NTA, as evidenced by the osteogenesis of encapsulated MSCs upon inductive culture. In summary, our study provided a biocompatible strategy to prepare alginate microgels for single-cell encapsulation which can be further used for applications in tissue engineering and cell therapies.
研究领域:
生物技术
·
组织工程
·
细胞生物学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 32
·26362-26370
·
IF 4.6
Han Wu,Jiayao Fang,Jiao Chen,Yaoqi Wang,Bo Zheng
Protein display technology enables high-throughput screening and plays an important role in protein discovery and engineering. Conventional in vivo display methods face challenges such as inefficient gene transformation and complex cell proliferation dynamics, while in vitro display methods are often limited to affinity-based selection and suffer from expression bias due to homogeneous reaction conditions. Here, we present a hydrogel particle-based protein display method enabled by particle-templated emulsification. This approach uses functionalized polyacrylamide hydrogel particles as isolated microreactors, incorporating DNA primers for genotype immobilization and Ni-NTA groups for capturing histidine-tagged protein phenotypes. Displayed proteins are synthesized via cell-free protein expression within isolated droplets, overcoming the limitations of in vivo cell culture and enabling compartmentalized screening, which is challenging in conventional homogeneous in vitro systems. Using particle-templated emulsification, single hydrogel particles can be rapidly encapsulated with individual DNA templates into isolated water-in-oil droplets within 30 seconds, without the need for specialized instrumentation. Up to 109 particles can be emulsified in a standard 50 ml conical tube. Compared to conventional droplet microfluidics, particle-templated emulsification achieves higher single-particle encapsulation and improved one-to-one particle–DNA pairing efficiency, reducing reagent consumption and minimizing DNA library loss caused by improper pairing. Digital PCR and cell-free protein expression are sequentially performed within droplets, with both the amplified DNA and the expressed protein immobilized on the same particle, thereby establishing a stable genotype–phenotype linkage. This method eliminates the need for cell handling, enables compartmentalized functional screening, and provides a fast, scalable, and user-friendly workflow for protein display, offering strong potential in directed evolution and protein engineering.
研究领域:
生物化学
·
生物技术
·
蛋白质工程
·
合成生物学
·
分子生物学
·
微流控技术
·
定向进化
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）