Molecular Medicine Reports
·
2019
·Vol. 19
·期 6
·4673-4684
·
IF 1.851
Yayun Liu,Lu Liao,Yong Chen,Fengmei Han
Non‑alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease, and has high rates of morbidity and mortality worldwide. Daphnetin (DAP) possesses notable antioxidative, anti‑inflammatory and anticoagulant activities; DAP is an active ingredient extracted from Daphne Koreana Nakai. To investigate the effects and the underlying mechanism of DAP on NAFLD, we treated HepG2 cells with oleic acid (OA) and DAP simultaneously and non‑simultaneously. In the simultaneous treatment condition, HepG2 cells were co‑treated with 0.5 mM OA and DAP (5, 20, and 50 µM) for 24 h. In the non‑simultaneous treatment conditions, HepG2 cells were pretreated with 0.5 mM OA for 24 h, and then treated with DAP (5, 20 and 50 µM) for 24 h. Following the aforementioned treatments, the biochemical indexes associated with NAFLD were measured as follows: i) The intracellular contents of triglyceride (TG), reactive oxygen species (ROS) and fluorescent glucose 2‑[N‑(7‑nitrobenz‑2‑oxa‑1,3‑diazol‑4‑yl) amino]‑2‑deoxyglucose were analyzed with corresponding detection kits; and ii) the cellular expression levels of glycolipid metabolism‑ and oxidative stress‑related genes, including 5'AMP‑activated protein kinase (AMPK), sterol regulatory element‑binding protein‑1C (SREBP‑1C), patatin‑like phospholipase domain‑containing protein 3 (PNPLA3), peroxisome proliferator‑activated receptor α (PPARα), phosphoinositide 3‑kinase (PI3K), protein kinase B (AKT), nuclear factor‑like 2 (Nrf2), cytochrome P450 (CYP) 2E1 and CYP4A were determined by reverse transcription‑quantitative polymerase chain reaction and western blotting. The results revealed the potential mechanism underlying the effects of DAP on NAFLD in vitro: i) By increasing the phosphorylation of AMPK, DAP inhibited the expression of SREBP‑1C and PNPLA3, and induced that of PPARα. Lipid accumulation within hepatocytes was reduced; ii) by upregulating PI3K expression and pAKT/AKT levels, DAP may alleviate insulin resistance and promote hepatocellular glucose uptake; and iii) by upregulating the expression of Nrf2, DAP downregulated the expression of CYP2E1 and CYP4A, and the levels of reactive oxygen species in hepatocytes.
研究领域:
药理学
·
细胞生物学
·
代谢性疾病
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DOI
PubMed
高影响因子
INFLAMMATION RESEARCH
·
2025
·Vol. 74
·期 1
·1-20
·
IF 5.4
Song Chao,Shen Baoxin,Chen Chaoqi,Yang Lei,Zhang Chi,Liu Fei,Chen Feng,Wu Xiaofei
Background Osteoarthritis (OA) is a common chronic degenerative joint disease in orthopedics, and ferroptosis is a newly identified mode of cell death present in OA. Inhibition of inflammatory cytokine expression and modulation of chondrocyte ferroptosis related pathways may be novel strategies for the treatment of OA. The purpose of this work was to uncover prospective biomarkers and molecular processes of ferroptosis in OA, as well as to better understand the molecular mechanisms of ferroptosis in OA treated with resveratrol. Material and methods We obtained OA gene expression profiles from the Gene Expression Omnibus (GEO) database. OA-expressed ferroptosis-related genes were identified using Genecards data, differential gene analysis, and weighted gene co-expression network analysis. Enrichment analysis was utilized to identify signaling pathways and molecular mechanisms linked with ferroptosis in OA, while immune infiltration analysis indicated immune cell infiltration in OA. The action targets of resveratrol were taken from the TCM database to determine the therapeutic targets of resveratrol for the treatment of OA. To validate the molecular process, molecular docking was performed using the therapeutic targets' enrichment analysis. Finally, in vitro investigations confirmed the molecular mechanism of ferroptosis in resveratrol-treated OA. Results Bioinformatic analysis identified 462 OA ferroptosis gene sets, with GPX4, TFRC, SLC7A11, EGFR, and IL1B serving as significant hub genes. Enrichment analysis revealed that ferroptosis was also linked to animal mitophagy, the FoxO signaling pathway, the Toll-like receptor signaling pathway, the PI3K-Akt signaling pathway, inflammation, immune response activation, and cellular autophagy. The immune infiltration data revealed that T_cells_CD4_memory_resting, T_cells_CD4_memory_activated, NK_cells_activated, and Mast_cells_activated were considerably infiltrated in OA. Resveratrol ameliorated OA via modulating autophagy and ferroptosis via GPX4, TFRC, SLC7A11, EGFR, and IL1B, according to a mechanistic study. Conclusion We discovered the mechanism of GPX4, TFRC, SLC7A11, and EGFR, IL1B ferroptosis-related genes in OA, and preliminary evidence suggests that resveratrol improves OA by regulating ferroptosis and immunological processes, which may give a new route for OA treatment.
研究领域:
医学
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生物信息学
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DOI
PubMed
高影响因子
JOURNAL OF VINYL & ADDITIVE TECHNOLOGY
·
2023
·Vol. 29
·期 6
·1058-1069
·
IF 2.7
Liangdong Ye,Hongbo Liu,Dacheng Li,Qiaoyan Wei,Ziwei Li,Dongming Chen,Shaorong Lu
The fabrication of composites of two or more materials with different polarities requires the use of compatibilisers to improve interface properties. However, most compatibilisers used for industrial production are derived from the cracking products of petroleum, which is a limited resource susceptible to price fluctuations and pollutes the environment. In this study, a new compatibiliser derived from renewable plant-oil-based products, ESO–G–OA, was designed and synthesized using epoxy soybean oil, oleic acid, and glycerol. Scanning electron microscopy results showed that ESO–G–OA can effectively improve the dispersion of CaCO 3 in a recycled high-density polyethylene (reHDPE) matrix and reduce the interfacial gap between the two phases. The analysis of the mechanical properties showed that the ESO–G–OA-modified composite has higher tensile and impact strength than unmodified samples. The ESO–G–OA modification improved the thermal stability and melt flow of the composite and reduced the energy consumption during processing. Moreover, the excellent compatibility of ESO–G–OA can improve the comprehensive properties of CaCO 3 /reHDPE composites, compensating for the performance reduction caused by the multiple processing steps necessary to obtain reHDPE. This confirmed that ESO–G–OA has promising application prospects in the production of composites requiring compatibilisers.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
PROTEOMICS
·
2024
·
IF 3.4
Chao He,Xiuyuan Chen,Ying Chen,Jianying Sun,Manting Qi,Sonia Rocha,Mu Wang
Prostate cancer (PCa) is one of the leading causes of cancer morbidity and mortality in men. Metastasis is the main cause of PCa-associated death. Recent evidence indicated a significant reduction in PCa mortality associated with higher ω-3 polyunsaturated fatty acids (PUFAs) consumption. However, the underlying mechanisms remained elusive. In this study, we applied global acetylome profiling to study the effect of fatty acids treatment. Results indicated that oleic acid (OA, monounsaturated fatty acid, MUFA, 100 µM) elevates while EPA (eicosapentaenoic acid, 100 µM) reduces the acetyl-CoA level, which alters the global acetylome. After treatment, two crucial cell motility regulators, PFN1 and FLNA, were found with altered acetylation levels. OA increased the acetylation of PFN1 and FLNA, whereas EPA decreased PFN1 acetylation level. Furthermore, OA promotes while EPA inhibits PCa migration and invasion. Immunofluorescence assay indicated that EPA impedes the formation of lamellipodia or filopodia through reduced localization of PFN1 and FLNA to the leading edge of cells. Therefore, perturbed acetylome may be one critical step in fatty acid-affected cancer cell motility. This study provides some new insights into the response of ω-3 PUFAs treatment and a better understanding of cancer cell migration and invasion modulation.
研究领域:
肿瘤学
·
生物化学
·
细胞生物学
·
营养学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Xueyi Guo,Weijia Wang,Ying Yang,Qinghua Tian,Yang Xiang,Yan Sun,Zhiming Bai
Magnetic nano capture agent (MNCA)-based magnetic separation can remove heavy metals from blood efficiently and safely. It has been considered as the next-generation therapy for heavy metal intoxication. In this work, we report the first use of MNCA to remove arsenic (As) with high efficiency from blood. MNCA with a flexible, non-covalent and biocompatible surface layer has been synthesized by packaging cetyltrimethylammonium bromide (CTAB) and bovine serum albumin (BSA) together with oleic acid coated magnetic iron oxide nanoparticles (MNP@OA) via Van der Waals force. This surface layer enhances arsenic anion diffusion from the surface to the binding sites, leading to increased arsenic removal performance of MNCA. The adsorption capacities of BSA and CTAB enfolded MNP@OA (MNP@CTAB-BSA) for As (III) and As (V) are 131.00 mg g −1 and 119.58 mg g −1 , respectively. 89.02% of As (III) and 81.78% of As (V) are removed from blood using MNP@CTAB-BSA, and the residual concentrations of As (III) and As (V) in blood decrease from 400.00 μg L −1 to 43.93 μg L −1 and 72.88 μg L −1 , respectively. The as-synthesized MNP@CTAB-BSA shows excellent biocompatibility. Under operation condition, only 0.80% of hemolysis and 1.64% of plasma protein adsorption are induced by MNP@CTAB-BSA, and the cell viability of peripheral blood mononuclear cells (PBMCs) is more than 95.53%. This study paves a fast, safe and effective way for arsenic intoxication therapy.
研究领域:
纳米技术
·
环境科学
·
毒理学
·
生物医学工程
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产品关联
DOI
高影响因子
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
·
2023
·
IF 3.9
Yinzhong Chen,Wei Guo,Weizhao Lu,Xiucheng Guo,Weilu Gao,Zongsheng Yin
Osteoarthritis (OA), the most common joint disease, is characterized by inflammation and cartilage degradation. Previous studies illustrated that Smad nuclear-interacting protein 1 (SNIP1) is an inhibitor of the TGF-β signal transduction pathway and SNIP1 has been reported as an anti-inflammatory factor. This study aimed to explore the role of SNIP1 in OA progression. In this study, the SNIP1 expression was evaluated in OA human and OA mice tissue and interleukin-1 beta (IL-1β)-induced chondrocytes . The Safranin-O (SO) staining and osteoarthritis research society international (OARSI) scoring system was used to evaluate cartilage injury. The gain- and loss-of-function studies for SNIP1 were performed in chondrocytes. The SNIP1 overexpression adenovirus was injected into mice by intra-articular injection. The SNIP1 expression was decreased in OA patients, OA mice, and IL-1β-stimulated chondrocytes. The cartilage injury of medial meniscus-induced OA (DMM-OA) mice at 8 weeks showed more severe than that at 4 weeks. The expression of SNIP1 was lower at 8 weeks than that at 4 weeks. In IL-1β-stimulated chondrocytes, SNIP1 overexpression reduced the expression of TNF-α and IL-6, alleviated ECM degradation, reduced the phosphorylation levels of p65 and IκBα , and decreased the p65 level in nuclear. Moreover, overexpression of SNIP1 alleviated cartilage injury in DMM-OA mice. In brief, our study suggested that SNIP1 alleviated OA and repressed inflammation by inhibiting the activation of NF-κB. This study might provide a new insight into OA treatment.
研究领域:
风湿病学
·
分子生物学
·
炎症
·
软骨生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·
IF 7.2
Wei Wang,Deping Xia,Xin Wang,Hualin Lin,Jingzhou Liu,Shuai Huang,Mingan Zhou,Bin Dai,Sheng Han
Traditional mineral oil-based cutting fluids are increasingly incompatible with sustainable manufacturing due to their environmental and health impacts. To solve these challenges, this work presented a green and scalable strategy for a high-performance nano-cutting fluid by constructing a plant-based Pickering emulsion. This system innovatively utilized cottonseed oil as the base oil, stabilized by oleylamine (OA) modified MoS 2 nanosheets (MoS 2 -OA) at the oil-water interface. The formulation was rationally designed and optimized by response surface methodology (RSM) to synergistically enhance emulsion stability, lubrication, and heat dissipation. The resulting Pickering emulsion is demonstrated to possess a unique combination of significantly reduced friction and wear, along with improved thermal conductivity, outperforming a commercial cutting fluid. Mechanistic analysis revealed that MoS 2 -OA nanosheets formed a multifunctional interfacial film, which synergistically served as a “lubrication film” for low friction and a “thermal bridge” for efficient heat dissipation. Thus, a novel paradigm for developing sustainable metalworking fluids is established by leveraging the synergistic potential of non-edible plant oils and functional nanomaterials.
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DOI
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 6
·1006
·
IF 5.1
Yiting He,Xiayu Zhu,Ajun Li,Shuyuan Lin,Bo Li,Songbin Liu,Xinyu Ye
CsPbBr3perovskite quantum dots (QDs) have attracted significant attention for optoelectronic applications owing to their outstanding optical properties, yet achieving controlled synthesis with high stability under mild conditions remains a challenge. The room-temperature synthesis of CsPbBr3perovskite quantum dots using a coprecipitation method is systematically investigated in this work, with an emphasis on how the structural and optical properties of the QDs are influenced by the Br/Pb ratio and OA/OAm ratio. The findings show that controlling the Br/Pb and OA/OAm ratios can effectively influence the size, crystalline phase, and surface passivation properties of CsPbBr3quantum dots. The photoluminescence peak shifts blue and the bandgap widens when the Br/Pb ratio rises due to a decrease in quantum dot size. This is mainly explained by more effective surface covering by Br−ions and increased quantum confinement effects. The resultant quantum dots demonstrate ideal optical performance at a Br/Pb ratio of 75 and an OA/OAm ratio of 1.5, with dense ligand coverage, superior defect passivation, and markedly improved stability under UV irradiation and in aqueous environments. Variations in the Br/Pb and OA/OAm ratios affect the binding configuration and coverage of ligands on the quantum dot surface, thereby influencing the relationship between non-radiative recombination and the quantum confinement effect. The LED fabricated with the as-synthesized high-performance quantum dots demonstrates a wide color gamut, covering 129.45% of the NTSC standard, indicating strong potential for display applications.
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2018
·Vol. 13
·期 2
·e0193023
·
IF 2.766
Yanjun Hua,Wei Qiu,Qiuyi Xiao,Qiang Wu
Purpose
To assess the precision (repeatability and reproducibility) of ocular parameters measured by the Tomey OA-2000 biometer, and to compare them with those measured by the IOLMaster.
Methods
In this prospective study, the right eyes of 108 healthy subjects were included. Three consecutive scans were obtained by 2 observers using the Tomey OA-2000, and in the same session one observer used the IOLMaster (version 5.4.4.0006) for the measurements. About 1 week later, 3 scans were obtained by one observer using the Tomey OA-2000. The axial length (AL), central corneal thickness (CCT), anterior chamber depth (ACD), lens thickness (LT), keratometer readings, pupil diameter (PD) and corneal diameter (CD) values measured by the Tomey OA-2000 and IOLMaster were analyzed. The coefficient of variation (CoV), intraclass correlation coefficient (ICC), within subject standard deviation (Sw) and 2.77Sw were calculated to assess the repeatability and reproducibility. The paired t test and Bland-Altman plots were used to analyze the differences and agreements of parameters measured by the two devices, respectively.
Results
Intraobserver repeatability, and interobserver and intersession reproducibility of the AL, CCT, ACD, LT, Kf, Ks, Km, PD and CD values measured by the Tomey OA-2000 biometer showed a CoV of less than 1% except that for PD, and an ICC of more than 0.97 except that for PD and CD. The AL, Kf, Ks, Km and CD values measured by the Tomey OA-2000 were 0.058 ± 0.094 mm, 0.088± 0.150 diopters (D), 0.163 ± 0.170 D, 0.127 ± 0.117 D and 0.171 ± 0.217 mm lower than those measured by the IOLMaster, respectively (all Ps < 0.05). However, the ACD values from the two devices were comparable (P = 0.169). The 95% linite of agreement (LoA) of the AL, ACD, CD and all keratometer readings were no more than 0.24 mm, 0.14 mm 0.60 mm and 0.5 D, respectively.
Conclusion
Except for the PD and CD, the ocular parameters measured by the Tomey OA-2000 were highly repeatable and reproducible. Except for the CD value, there was good agreement of ocular parameters measured by the Tomey OA-2000 and the IOLMaster in healthy eyes.
研究领域:
眼科学
·
生物医学测量
·
屈光手术
·
白内障手术
·
光学生物测量
✓ 已验证
DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2021
·Vol. 32
·期 11
·4516-4530
·
IF 3.665
Pengfei Jia,Wenhua Cheng,Jingyi Lu,Zhenting Yin,Zhoumei Xu,Liang Cheng,Yong Qiu,Lijun Qian,Yuan Hu,Weizhao Hu,Bibo Wang
Flexible polyurethane foam (FPUF) is extremely flammable and will release a large number of toxic fumes during the combustion process, which brings great safety hazards. Therefore, a binary hybrid nanocoating composed of graphene oxide (GO) and octaamino polyhedral oligomeric silsesquioxane (OA-POSS) is deposited on the surface of FPUF through layer-by-layer self-assembly technology, with the aim of enhance the flame retardancy and smoke suppression properties of the hybrid FPUF. Compared with pure FPUF, the peak heat release rate, the total heat release, the peak smoke production rate and the total smoke release of FPUF@GO/OA-POSS-6 is decreased by 67.3%, 28.3%, 65.03% and 42.9%, indicating the hybrid FPUF excellent flame retardancy and smoke suppression properties. Compared with pure FPUF, the tensile strength of FPUF@GO/OA-POSS-6 is increased by 42%, and after three times of compression, the compressive strength increases by 83.17% on average. Facts have proved that the hybrid effect between GO and OA-POSS enhances the flame retardancy, smoke suppressive effects, tensile strength and compressive strength of FPUF.
研究领域:
材料科学
·
纳米技术
·
燃烧科学
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产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 5
·2402-2411
·
IF 3.049
Maoxin Wang,Bo Zhang,Gongrang Li,Tao Wu,Dejun Sun
Crude oil contaminated soil has been widely recognized to constitute a major environmental issue due its adverse effects on human health and ecological safety. The main objective of this study is to explore the possibility of using an ex situ solvent/surfactant washing technique for the remediation of crude oil-contaminated soil. Three organic solvents (methanol, acetone, and toluene) and one surfactant (AES-D-OA) were employed to form three kinds of solvent/surfactant systems, and utilized to evaluate the desorption performance of crude oil from soil. Natural soil, crude oil-contaminated soil, and after-remediation soil were characterized by SEM, EDX, FT-IR, and contact angle. The ability of solvent/surfactant systems to remove crude oil from soil was determined as a function of solvent polarity, mass ratio of solvent to surfactant, temperature, and ionic strength. The removal of crude oil by the toluene/AES-D-OA system was found to be more effective than the other systems. At a high toluene ratio, more than 97% of crude oil could be removed from contaminated soil. Crude oil removal efficiency was also found to increase with rising temperature or increasing ionic strength appropriately. Experimental results suggested that, compared to conventional surfactant-aided remediation, the combined utilization of surfactant and solvent achieved superior results for crude oil removal because of their similar compositions and structures in terms of aromaticity and polarity.
研究领域:
环境科学
·
土壤修复技术
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产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2026
·Vol. 37
·期 2
·e70546
·
IF 3.8
Jing Wang,Xin Qian,Keyu Shi,Yao Wu,Yonggang Zhang,YuSen Liu,Tao Yu
CFRP composites face limited application due to weak interfacial bonding, especially for ultrahigh modulus carbon fibers (CFs) with a tensile modulus exceeding 500 GPa. In this study, a novel approach involving the grafting of octaaminopropyl polyhedral oligomeric silsesquioxane (OA-POSS) nanoparticles onto ultrahigh modulus CFs was developed to optimize the interfacial bonding with ultrahigh modulus CF and epoxy resin (EP). Comprehensive characterization demonstrated that OA-POSS modification significantly altered the physicochemical structure of the fiber surface; for example, surface roughness increased from 10.3 to 18.1 nm, and the O/C ratio increased significantly from 0.060 to 0.232, confirming the successful introduction of abundant polar functional groups by OA-POSS. The CF-POSS/EP composites exhibited a peak compressive strength of 282.60 MPa (at 3 wt% POSS) and an interlaminar shear strength of 63.05 MPa (at 2 wt% POSS), corresponding to improvements of 31.50% and 20.70%, respectively, over the untreated samples. The performance improvement stems from OA-POSS nanoparticles forming covalent bonds via reactive amino groups, enhancing mechanical interlocking through increased surface roughness, and acting as a stress-relief interphase to promote crack deflection and energy dissipation. This altered the failure mechanism from interfacial separation to matrix internal cracking, enabling more efficient stress transfer across the interface.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）