ANALYST
·
2024
·
IF 3.6
Chengchen Tan,Xiaolong Yan,Xingchang Lu,Jianxiu Wang,Xinyao Yi
Breast cancer, as a malignant tumor with the highest incidence in the world, poses a great threat to human life and health. Early diagnosis of breast cancer may help enhancing the survival rate. In this work, a colorimetric and fluorescent dual-mode biosensor based on CRISPR-Cas12a system was constructed for the detection of breast cancer biomarker BRCA1. The intact G4 DNA could form DNAzyme in the assistance of K+ and hemin, catalyzed the oxidation of o-phenylenediamine (OPD) under the assistance of hydrogen peroxide (H2O2), and generate the oxidation product 2,3-diaminophenazine (DAP) with obvious absorption and fluorescence peaks. The presence of the target BRCA1 can activate the trans-cleavage activity of CRISPR-Cas12a, cleaving the G4 DNA and inhibiting the catalytic oxidation of OPD. The target BRCA1 was quantitatively determined by detecting both the absorbance and fluorescence intensity of DAP. Compared with single signal analysis, dual-signal output mode provided more reliable detection results. The detection limits were calculated to be 0.615 nM for colorimetric method and 0.289 nM for fluorescence method. The dual-mode biosensor showed good selectivity for BRCA1 and can resist the interference of complex substrates, which has great potential in biomedical detection.
研究领域:
生物医学工程
·
分子生物学
·
癌症研究
·
生物技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Xiaoyan Sun,Yuxin Guo,Ruizhi Wen,Hongchang Li
Cu 2+ is a major environmental pollutant. An efficient measurement for Cu 2+ is urgently needed. In this report, we have developed a new sensitive and selective ratiometric sensing platform using 7-amino-4-methylcoumarin (AMC) for detecting Cu 2+ in real samples. In the presence of Cu 2+ , o-phenylenediamine (OPD) could be catalytically oxidized to 2,3-diaminophenazine (DAP), which could react with AMC, leading to quench the fluorescence intensity of AMC at 438 nm. Meanwhile, DAP provided a new emission peak at 557 nm. Based on the efficient overlapped spectrum of AMC and DAP, a ratiometric sensing platform through fluorescence resonance energy transfer (FRET) was carried out. Furthermore, the as-proposed strategy displayed the linear relationship in the wide range from 6 to 250 μM with a low detection limit of 0.059 μM, and the recoveries of the spiked samples in real samples ranged from 86.5% to 110.1%. Moreover, comparing the visual fluorescence colors of the real samples with the standard colorimetric card, we used the as-proposed strategy as a solid-based platform for realizing an efficient semi-quantitative detection of Cu 2+ via naked-eye visual fluorescence mode without any complicated instrument and operation. The above results implied that the as-proposed strategy could be used in the practice determination of Cu 2+ .
研究领域:
环境科学
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分析化学
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光谱学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Chengna Xu,Ji Zhou,Yong Ye,Bin Tang
Peroxidase-like reaction process involving o-phenylenediamine (OPD) and silver nanoprisms in the presence of hydrogen peroxide (H 2 O 2 ) was monitored using time-resolved ultraviolet–visible (UV–Vis) absorption spectroscopy. The oxidation of OPD and etching of silver nanoprisms were investigated by analyzing the dynamic spectral data. Two-dimensional correlation spectroscopy (2D-COS) and principal component analysis (PCA) were employed to gain insights into the correlation between catalytic oxidation of OPD and etching of silver nanoprisms. It was found that OPD offered significant protection effect for silver nanoprisms so that morphologies of silver nanoprisms maintained at the beginning period after addition of H 2 O 2 . Moreover, silver nanoprisms accelerated the oxidation of OPD by H 2 O 2 , demonstrating enzymatic mimicking activity of silver nanoprisms. The combination of time-resolved UV–Vis absorption spectroscopy and spectral calculation methods could be used for exploration of complex reaction systems with spectral variations.
研究领域:
纳米技术
·
催化
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光谱学
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材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2024
·Vol. 24
·期 4
·1093
·
IF 3.4
Zhen Li,Yang Hu,Liu Wang,Houfang Liu,Tianling Ren,Cong Wang,Daoliang Li
A surface-enhanced Raman scattering (SERS) method for measuring nitrate nitrogen in aquaculture water was developed using a substrate of β-cyclodextrin-modified gold nanoparticles (SH-β-CD@AuNPs). Addressing the issues of low sensitivity, narrow linear range, and relatively poor selectivity of single metal nanoparticles in the SERS detection of nitrate nitrogen, we combined metal nanoparticles with cyclodextrin supramolecular compounds to prepare a AuNPs substrate enveloped by cyclodextrin, which exhibits ultra-high selectivity and Raman activity. Subsequently, vanadium(III) chloride was used to convert nitrate ions into nitrite ions. The adsorption mechanism between the reaction product benzotriazole (BTAH) of o-phenylenediamine (OPD) and nitrite ions on the SH-β-CD@AuNPs substrate was studied through SERS, achieving the simultaneous detection of nitrate nitrogen and nitrite nitrogen. The experimental results show that BTAH exhibits distinct SERS characteristic peaks at 1168, 1240, 1375, and 1600 cm−1, with the lowest detection limits of 3.33 × 10−2, 5.84 × 10−2, 2.40 × 10−2, and 1.05 × 10−2μmol/L, respectively, and a linear range of 0.1–30.0 μmol/L. The proposed method provides an effective tool for the selective and accurate online detection of nitrite and nitrate nitrogen in aquaculture water.
研究领域:
分析化学
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环境科学
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纳米技术
·
水产养殖
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-10
·
IF 6.232
Chen Xiaolong,Wang Xianfeng,Cao Gaihua,Wu Yawen,Luo Huibo,Ji Zhong,Shen Caihong,Huo Danqun,Hou Changjun
Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of H 2 O 2 . DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF’s peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively. Graphical abstract
研究领域:
材料科学
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分析化学
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生物化学
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DOI
PubMed
高影响因子
Nanoscale Advances
·
2023
·
IF 4.7
Guo-Ying Chen,Mao-Ling Luo,Li Chen,Tong-Qing Chai,Jia-Li Wang,Ling-Xiao Chen,Feng-Qing Yang
The combined application of nanozymes and natural enzymes has received widespread attention in recent years. In this work, a simple and efficient method was used to synthesize a composite material of CuO nanoparticles modified zeolitic imidazolate framework-8 (CuO NPs@ZIF-8) with multiple enzyme activities (glucose oxidase-like and hydrolase-like activities) to detect the activity of natural enzymes through fluorescence and colorimetric (UV-Vis) dual-mode detections. The hydrolase- and oxidase-like activities of CuO NPs@ZIF-8 show an acceptable affinity with L-ascorbic acid 2-phosphate trisodium (AAP) and o-phenylenediamine (OPD). Using the developed sensor, highly sensitive detection of natural enzymes glucose oxidase (GOX) and alkaline phosphatase (ALP) was achieved through both fluorescent and colorimetric analysis with a wide linear range (fluorescence for GOX: 0.86-1.23×105 mU/mL, UV-Vis for GOX: 0.081-1.62×105 mU/mL; fluorescence for ALP: 0.042-1.20×104 mU/mL, UV-Vis for ALP: 0.0046-1.23×104 mU/mL) and low LOQs (fluorescence for GOX: 0.86 mU/mL, UV-Vis for GOX: 0.081 mU/mL; fluorescence for ALP: 0.042 mU/mL, UV-Vis for ALP: 0.0046 mU/mL). Compared to the other fluorescent and colorimetric sensors, this sensor has better catalytic activity due to the addition of GOX and ALP, which can amplify the detection signal and improve the sensitivity. This is the first time that composite material CuO NPs@ZIF-8 with "tandem enzyme" activity was synthesized and applied in the detection of enzyme activity. Additionally, the proposed fluorescent and UV-Vis platforms exhibit the capability to detect GOX and ALP in serum samples with satisfactory recovery, indicating potential application prospects in biochemical analysis.
研究领域:
分析化学
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生物化学
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纳米技术
·
材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shi Gang Liu,Lei Han,Na Li,Yu Zhu Fan,Yu Zhu Yang,Nian Bing Li,Hong Qun Luo
The development of ratiometric fluorescent strategy is very significant and challenging in bioanalysis. Herein, graphitic carbon nitride (g-C 3 N 4 ) nanosheets and cobalt oxyhydroxide (CoOOH) nanoflakes are exploited for ratiometric fluorescence assay of alkaline phosphatase (ALP) activity. In the g-C 3 N 4 /CoOOH nanohybrid, g-C 3 N 4 nanosheets serve as a signal unit and the CoOOH nanoflakes function as a recognition element, and initially, the fluorescence of g-C 3 N 4 is quenched by the CoOOH nanoflakes. In the absence of target, the CoOOH nanoflakes of the nanohybrid system are able to oxidize o-phenylenediamine (OPD), and the resultant oxidation product (OxOPD) quenches the blue emission of g-C 3 N 4 and meanwhile emits orange fluorescence which acts as another signal element. However, an efficient redox reaction between ascorbic acid (AA) and CoOOH can cause decomposition of the CoOOH nanoflakes, and additionally, ALP can catalytically hydrolyze L-ascorbic acid-2-phosphate (AAP) to generate AA. Thus, in the presence of target, the CoOOH nanoflakes are destroyed by AA preferentially and the OPD is rarely oxidized to OxOPD, accompanied with strong blue emission of g-C 3 N 4 and weak orange fluorescence from OxOPD. Target-dependent dual-signal change made the ratiometric assay possible, and also AA-induced signal variation was investigated and attributed to the stronger reducing capacity of AA than OPD. The ratiometric sensing platform for ALP activity assay provides a new perspective for the applications of two-dimensional nanomaterials to develop novel and sensitive biosensors.
研究领域:
生物化学
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分析化学
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纳米技术
·
生物医学工程
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产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2023
·Vol. 39
·期 6
·935-943
·
IF 1.6
Wang Jingyu,Cui Yunyi,Lin Ying,He Yi
Abstract A long-standing challenge in colorimetric detection of triacetone triperoxide (TATP) explosive is low sensitivity. We herein developed an iodine-mediated photoinduced auto-inductive tandem chromogenic system to achieve exponential signal amplification. The strategy employs the KI-TATP reaction and photo-induced autocatalytical oxidation of o-phenylenediamine (OPD) that work in tandem. The resulting I 3 − from the KI-TATP reaction oxidizes OPD to yellow 2,3-diaminophenazine (DAP) that is further excited by blue light illumination to produce reactive oxygen species (ROS). The obtained ROS, in turn, promotes the oxidation of OPD to gain more DAP, causing the auto-inductive chromogenic reaction processes. This tandem chromogenic system is applied for visual colorimetric detection of TATP, allowing the selective and sensitive detection of TATP down to 42.8 μM. Moreover, analyses of TATP in real samples are performed, and the satisfactory recovery results are achieved. Furthermore, a field detection kit is also developed. Graphical abstract
研究领域:
分析化学
·
爆炸物检测
·
光化学
·
比色传感器
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 29
·13963-13970
·
IF 3.925
Pengli Li,Min Wang,Mingzhe Jiang,Wenjing Lai,Jiajia Li,Kailin Liu,Hongling Li,Chenglin Hong
The early detection of tumour markers is essential for the timely treatment of cancer, and thus realising the sensitive detection of carcinoembryonic antigen (CEA) is significant. Herein, we employed an electrochemical immunosensor as a simple analytical method. The loading of precious metals significantly amplifies the signal of electrochemical immunosensors. In this study, the successful loading of Au NPs was achieved via the galvanic displacement method. Moreover, CuS nanoflowers possessed intrinsic peroxidase-like activity, which could catalyse the oxidation of o-phenylenediamine (OPD) to generate a light-orange product in the presence of hydrogen peroxide (H2O2). The flower-shaped CuS not only provided active sites for the catalytic reaction, but also possessed a large specific surface area, which could provide abundant sites for the placement of Au nanoparticles. Thus, it was suitable for the labelling of Ab2 for the sensitive detection of CEA. The instant current response (i–t) method detected CEA quantitatively, and the immunosensor prepared under the optimized conditions exhibited an excellent analytical performance. The detection range of the sensor prepared in this study was 0.1–80 ng mL−1 and its detection limit was 5.5 pg mL−1 (S/N = 3).
研究领域:
生物传感器
·
纳米技术
·
癌症诊断
·
电化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Ning Xia,Gang Liu,Shuo Zhang,Zichen Shang,Yinpei Yang,Yuanyuan Li,Lin Liu
This work reported that the peptide-Cu 2+ complexes reveal oxidase-mimicking activities for oxygen reduction and ascorbate oxidation . The peptide features a histidine (His) residue in the first position with respect to the free N-terminal amine. The complexes can be easily integrated with nanomaterials such as gold nanoparticles (AuNPs) to form nanocatalysts or nanozymes, which allows for the fabrication of sensitive sensing platforms. To explore the analytical performances of the peptide-Cu 2+ complexes in biosensing, sandwich electrochemical and fluorescent immunoassays of prostate specific antigen (PSA) were performed with AuNP/peptide-Cu 2+ conjugates as the nanolabels. In the electrochemical immunoassays, the nanolabels coined as nanocatalysts promoted the electrocatalytic reduction of oxygen. For the fluorescent assays of PSA, the nanolabels coined as nanozymes catalyzed the oxidation of ascorbic acid (AA), and the resulting oxidization product dehydroascorbic acid (DHA) was reacted with o-phenylenediamine (OPD) to form fluorescent 3-(dihydroxyethyl)furo [3,4-b]quinoxaline-1-one (DFQ). The electrochemical and fluorescent signals are proportional to the concentration of PSA in the linear range of 0.001–0.50 and 0.001–2.00 ng/mL, respectively. The low detection limits of the methods (0.40 and 1.00 pg/mL) enabled clinical detection of PSA in serum samples. The findings should be valuable for understanding the biological functions of peptide-Cu 2+ complexes, and provide new insight to develop optical and electrochemical sensing platforms for clinical diagnosis.
研究领域:
生物化学
·
分析化学
·
生物传感器
·
临床诊断
·
电化学
·
荧光分析
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）