Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2024
·
IF 2.3
Xin Wang,Xiaolei Zhao,Xinyi Song,Jinxing He
Ochratoxin A (OTA) is a toxic fungal metabolite that is commonly found in cereals and animal feed. It is economically damaging and potentially hazardous to human health. Herein, we propose an electrochemical immunosensor for the rapid detection of OTA using anti-OTA antibodies and diazonium-functionalized, screen-printed electrodes. We attached 4-aminobenzoic acid to an electrode surface, activated the carboxyl groups on the surface with carbodiimide, and attached an antibody to the diazo layer. Subsequently, we used bovine serum protein as a blocker to prevent non-specific antigens from binding to the antibody. We evaluated the performance of the sensor by cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry. The sensor is highly specific and sensitive, has good linear responses in the range 20–200 ng/mL, a limit of detection of 0.5 ng/mL, and good recoveries of 90.5%–100.9% in spiked samples. It can be stored at 4 °C for approximately 2 weeks, and is highly stable, with a current response variation of no more than 4.6%.
研究领域:
分析化学
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食品科学
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毒理学
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传感器技术
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yumei Hu,Longhai Dai,Yuhang Xu,Du Niu,Xuechun Yang,Zhenzhen Xie,Panpan Shen,Xian Li,Hao Li,Lilan Zhang,Jian Min,Rey-Ting Guo,Chun-Chi Chen
Ochratoxin A (OTA) contamination in various agro-products poses a serious threat to the global food safety and human health, leading to enormous economic losses. Enzyme-mediated OTA degradation is an appealing strategy, and the search for more efficient enzymes is a prerequisite for achieving this goal. Here, a novel amidohydrolase, termed Pw ADH, was demonstrated to exhibit 7.3-fold higher activity than that of the most efficient OTA-degrading ADH3 previously reported. Cryo-electron microscopy structure analysis indicated that additional hydrogen-bond interactions among OTA and the adjacent residue H163, the more compact substrate-binding pocket, and the wider entry to the substrate-access cavity might account for the more efficient OTA-degrading activity of Pw ADH compared with that of ADH3. We conducted a structure-guided rational design of Pw ADH and obtained an upgraded variant, G88D, whose OTA-degrading activity was elevated by 1.2-fold. In addition, Pw ADH and the upgraded G88D were successfully expressed in the industrial yeast Pichia pastoris , and their catalytic activities were compared to those of their counterparts produced in E. coli , revealing the feasibility of producing Pw ADH and its variants in industrial yeast strains. These results illustrate the structural basis of a novel, efficient OTA-degrading amidohydrolase and will be beneficial for the development of high-efficiency OTA-degrading approaches.
研究领域:
食品科学
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生物化学
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结构生物学
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Meng Xu,Yimeng Li,Xiaopeng Hu,Shuai Zhang,Yiwei Liu
A smartphone-assisted colorimetric aptasensor was constructed for the detection of ochratoxin A (OTA) utilizing Au/CeO₂/rGO nanohybrid. The sensor integrates aptamer-functionalized Au/Fe 3 O 4 (Apt/Au/Fe 3 O 4 ) as recognition elements. The complementary-chain-modified Au/CeO₂/rGO (cDNA/Au/CeO₂/rGO) act as nanozyme catalyst for signal amplification through the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-colored ox-TMB. The detection mechanism relies on competitive binding between OTA and cDNA/Au/CeO 2 /rGO to Apt/Au/Fe 3 O 4 . Upon OTA introduction, OTA preferentially binds to Apt/Au/Fe 3 O 4 . These Apt/Au/Fe 3 O 4 are magnetically separated and introduced into the cDNA/Au/CeO 2 /rGO suspension. Unbound Apt/Au/Fe 3 O 4 interacts with cDNA/Au/CeO 2 /rGO. After magnetic separation, the residual cDNA/Au/CeO 2 /rGO concentration in supernatant exhibits a correlation with the OTA concentration. This design effectively eliminates potential interference from the intrinsic color of coffee, significantly improving the accuracy of colorimetric detection. The aptasensor demonstrated superior selectivity towards OTA with a detection limit of 27.3 pg mL −1 . This specificity was validated against interference, with recovery rates of 102.1–117.4 % in coffee, as confirmed by ELISA.
研究领域:
食品安全
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分析化学
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纳米技术
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生物传感器
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毒素检测
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适配体技术
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 3
·382-389
·
IF 1.071
Wang Chengke,Tan Rong,Li Jiangyu,Zhang Zexiang
A double magnetic separation-assisted fluorescence method was developed to rapidly detect ochratoxin A(OTA). The OTA aptamer functionalized magnetic nanomaterial(Fe 3 O 4 -Aptamer) and complementary DNA conjugated nitrogen-doped graphene quantum dots(NGQDs-cDNA) were used in this assay. Aptamer could hybridize with cDNA, which induced the NGQDs-cDNA to bind onto Fe 3 O 4 -Aptamer, and resulted in the fluorescence quenching of NGQDs. After the addition of OTA, the NGQDs-cDNA could release into the solution, and resulted in the recovery of fluorescence signal of NGQDs consequently. By utilizing the magnetic separation, the unbonded NGQDs-cDNA and residual Fe3O4-Aptamer were removed, which significantly increased the fluorescence signal intensity. OTA could be detected in the linear range of 10 nmol/L to 2000 nmol/L, with a limit of detection as 0.66 nmol/L. The advantages of this method include simple operation, good selectivity and high sensitivity, and this method can be used for the rapid detection of ochratoxin A in wheat and corn.
研究领域:
食品科学
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分析化学
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Xin Yang,Yuanyuan Wang,Xiaowei Chen,Yujun Feng,Yingli Guo,Hualin Yang,Yu Zhou
Ochratoxin A (OTA) is widely present in the environment and has great harm to human safety. Therefore, a simple and sensitive method for OTA detection is urgently needed. Herein, a fluorescence and colorimetric dual-mode immunoassay based on glutathione silver nanocluster (GSH-AgNCs) and phosphate colorimetry was established. For fluorescence mode, the pyrophosphate ion (PPi) can reduce the fluorescence of GSH-AgNCs@Al 3+ by chelating Al 3+ . Meanwhile, the fluorescence can be recovered when PPi is hydrolyzed to phosphate ion (Pi) by alkaline phosphatase (ALP.) For colorimetric mode, Pi can be quantitatively analyzed by Mo Sb colorimetric method. The linear range of fluorescence mode was 1.25–35 ng/mL with limit of detection (LOD) of 0.54 ng/mL. For colorimetric mode, the linear range and LOD was 6.25–250 ng/mL and 3.13 ng/mL. The detection limits are much lower than the European Union's regulations. And this dual-mode immunoassay showed a satisfactory recovery rate and specificity in detecting real samples.
研究领域:
分析化学
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食品安全
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生物医学
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JOURNAL OF POLYMER RESEARCH
·
2022
·Vol. 29
·期 5
·1-11
·
IF 3.061
Wang Yulin,Li Yangyang,Luo Yiling,Zhou Kailian,Qiu Xiuzhen,Guo Huishi
A novel molecularly imprinted polymer composite material (OTA-MIPs) was prepared by the complexation between a metal–organic framework single-crystal ordered macropore ZIF-8 and a novel dummy template for the selective recognition of ochratoxin A (OTA). First, the structural analog of OTA was synthesized as a dummy template molecule. Then, OTA-MIPs were synthesized on the ZIF-8 surface by thermal polymerization. The as-synthesized OTA-MIPs were characterized by infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, and N 2 adsorption. The material exhibited a good network structure as well as a high specific surface area and pore size to facilitate the adsorption of target molecules. OTA-MIPs were examined for OTA re-binding properties. The Langmuir model fitted the adsorption data for OTA-MIPs better than the Freundlich model. At the same time, compared to the corresponding non-imprinted (OTA-NIPs) material, OTA-MIPs exhibited a 6.8-fold higher affinity for OTA. The molecular recognition ability of OTA-MIPs was highly specific for OTA compared to structurally similar compounds such as patulin and zearalenone. OTA-MIPs only exhibited a slight downward trend in terms of performance after five times of reuse. These results highlighted their potential for the concentration of OTA from complex food matrices.
研究领域:
食品科学
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分析化学
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ANALYTICAL BIOCHEMISTRY
·
2021
·
IF 3.365
Jingming Zhou,Zhanxiang Liu,Qingbao Yang,Wenjing Qian,Yumei Chen,Yanhua Qi,Aiping Wang
A sensitive and accurate multiple fluorescence immunoassay for the simultaneous quantitative detection of Zearalenone (ZEN) and Ochratoxin A (OTA) in single spot based on multicolor quantum dots (QDs) labeling was developed for the first time. Two kinds of ZnCdSe/ZnS (core/shell) QDs with maximum emission wavelengths at 520 nm (green) and 610 nm (orange-red) were selected as marking materials, respectively. The anti-ZEN-mAb-QDs and anti-OTA-mAb-QDs were designed as the immune fluorescent probes . Fluorescence was measured at the same excitation wavelength and two different emission wavelengths to determine each target. The procedure for QDs-based multiple fluorescence labeled immunosorbent assay (M-FLISA) was developed. The 50% inhibition concentrations (IC 50 ) of ZEN and OTA were 0.034 and 1.175 ng/mL. Moreover, the limits of detection (LOD) for the simultaneous determination were 0.0239 and 2.339 ng/g for ZEN and OTA in maize, respectively. In addition, the recoveries ranged from 93.15 to 101.90% for ZEN and from 95.29 to 102.43% for OTA, with the coefficient variation (CV) of 2.70–8.86% and 3.51–6.22% respectively. There was good consistency between the M-FLISA and high performance liquid chromatography (HPLC) results, which confirmed that the M-FLISA was suitable for the simultaneous quantitative detection of various mycotoxins .
研究领域:
分析化学
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毒理学
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食品安全
·
生物技术
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DOI
PubMed
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Guizhu Wu,Guangyun Chen,Junjian Li,Zhaoying Liu,Zhanming Li,Lijuan Hou,Zhen Zhang,Ming Li
A tri-mode aptasensor for ultrasensitive detection of ochratoxin A (OTA) was developed, based on two-dimensional hybridization chain reaction (2D-HCR)-guided etching to modulate gold nanobipyramid (AuNB) morphology. The OTA aptamer-functionalized magnetic probe (MB@2D-HCR@HRP) integrated the self-assembly 2D-HCR networks, which had loaded with abundant horseradish peroxidase (HRP) for cascade signal amplification. Following OTA recognition, the released 2D-HCR@HRP could induce the generation of hydroxyl radical (·OH), then perform the AuNB etching. The results of AuNB morphological transformation identified through three readouts: (1) visual multicolor (LOD of 100 pg mL −1 ), (2) surface plasmon resonance peak (LOD of 75.3 pg mL −1 ), (3) photothermal response (LOD of 14.7 pg mL −1 ). Thus, the levels of OTA could directly reflect via triple signals, achieving rapid and ultrasensitive detection within 45 min. The aptasensor exhibited high specificity (cross-reactivity less than 7.22 %) and reliability for spiked and authentic agricultural byproduct samples. Furthermore, the highlights of simplified operation visualization and portability had displayed. This aptasensor had handled advanced strategies to improve the detection performance, which might further promote the practicability and accessibility of biosensing.
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TALANTA
·
2025
·
IF 6.1
Kaisong Xu,Ziqiang Li,Jingshuang Zhang,Yuting Zhu,Qianjin Li,Jianlin Li
Raman Fingerprinting spectra detection of ochratoxin A (OTA) in food still meets serious challenge because of uncontrolled signal heterogeneity and poor reproducibility at low analyte concentrations. Here, OTA molecularly imprinted three-dimensional (3D) porous SERS substrate was prepared on the surfaces of magnetic inverse photonic crystal microspheres (MIPCMs) @AuNPs, and its Raman analytical enhancement factor can reach to 2.67 × 10 7 . The OTA imprinted polymer was synthesized using 3-aminopropyltriethoxysilane and phenyltriethoxysilane as the dual-functional monomers and Fmoc- d -Phenylalanine as the template molecule on the surfaces of MIPCMs. The functional microspheres can specifically enrich OTA and remarkably enhance the fingerprint Raman spectrum of OTA. The label-free 3D SERS substrate can rapidly quantitatively detect OTA by its fingerprint Raman spectrum, which shows a linear detection range of 10 −1 to 10 3 ng/mL, a limit of detection of 0.078 ng/mL, good recovery rates of 76–97 % and regeneration property. The developed SERS method can integrate enrichment and detection of OTA in one system, which has a great application prospect in simple, rapid and high sensitivity detection of OTA.
研究领域:
食品安全检测
·
分析化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Fengyang Wang,Yan Qi,Lei Ji,Fanglin Qiao,Yin Chen,Xiaohui Xiong,Yuanjian Liu
A GPE-PET (graphene-polyethylene terephthalate) bipolar electrode-electrochemiluminescence (BPE-ECL) platform was developed for ochratoxin A (OTA) detection. PET served as the electrode sheet substrate, and GPE was drop-coated onto the surface of PET to form a conductive line. On the functional sensing interface, the thiol (-SH) modified OTA aptamer (OTA-Aptamer) are fixed on the surface of the gold-plated cathode through Au S bonds. The efficient electron transfer ability of methylene blue (MB) made the anode ECL signal strong. Due to competition between OTA and MB with OTA-Aptamer, leading to a decrease in ECL intensity of the [Ru(bpy) 3 ] 2+ /TPA system on the BPE anode. Under optimized conditions, the GPE-PET BPE-ECL biosensor displayed superior sensitivity for OTA with a detection limit of 2 ng mL −1 and a wide linear concentration range of 5–100 ng mL −1 . This method could be further applied to detect various toxins and had broad application prospects.
研究领域:
分析化学
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食品安全
·
生物传感
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ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Cuiying Lin,Huixia Zheng,Mi Sun,Yajuan Guo,Fang Luo,Longhua Guo,Bin Qiu,Zhenyu Lin,Guonan Chen
A simple, low-cost, and sensitive liposome-based colorimetric aptasensor has been developed to detect ochratoxin A (OTA). Specifically, a dumbbell-shaped probe was designed, including magnetic beads (MBs), double-stranded DNA (dsDNA), and enzyme-encapsulated liposome. The dsDNA formed by the hybridization between OTA aptamer and its complementary probe. And the dsDNA was used to contact the MBs and the enzyme-encapsulated liposome. In the presence of OTA, the aptamer preferred to combine with OTA to form G-quadruplex, resulting in the release of the detection probe and the enzyme-encapsulated liposome. Each liposome contained a large amount of HRP. Thus, when the liposome was lysed by adding the mixed solution of 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 , a large number of HRP were released. HRP could catalyze the H 2 O 2 -mediated oxidation of TMB and hence resulted in the color change from colorless to blue with the OTA concentration varying, and this variation can be observed by naked eyes easily. The result showed that the absorption intensity at 652 nm enhanced with the increase of OTA concentration ranging from 0.05 to 2.0 ng mL −1 , and the limit of detection was calculated to be 0.023 ng mL −1 (S/N = 3). The developed colorimetric aptasensor has been applied to detect OTA concentration in corn samples with satisfied results.
研究领域:
食品科学
·
分析化学
·
生物技术
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DOI
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BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Juan Tang,Yapei Huang,Cengceng Zhang,Huiqiong Liu,Dianping Tang
A new impedimetric immunosensor for the fast determination of ochratoxin A (OTA) in food samples was developed based on the instant catalyst as enhancer. Initially, the signal tags were prepared via co-immobilization of anti -OTA antibody and amine-terminated dendrimer (PAMAM) on the graphene oxide nanosheets through the covalent interaction, which were utilized as a good platform for combining manganese ion ( anti -OTA-GO-PAMAM-Mn 2+ ). Upon target OTA introduction, a competitive-type immunoreaction was implemented between the analyte and the immobilized OTA-BSA on the electrode for the anti -OTA antibody on the graphene oxide nanosheets labels. After a competitive immunoassay format, the anti -OTA-GO-PAMAM-Mn 2+ were captured onto the electrode surface , which could induce the in situ formation of MnO 2 via classical redox reaction between Mn 2+ and KMnO 4 on the immunesensing platform. Moreover, the generated MnO 2 nanoparticles act as efficient catalyst could catalyze the 4-chloro-1-naphthol (4-CN) oxidation without H 2 O 2 to generate an insoluble precipitation on the platform. Under the optimal conditions, the instant catalyst based impedimetric immunosensor displayed a wide dynamic working range between 0.1 pg mL −1 and 30 ng mL −1 . The detection limit (LOD) of the assay was 0.055 pg mL −1 . The developed method exhibited high selectivity and can be used for the determination of OTA in real red wine samples.
研究领域:
食品科学
·
分析化学
·
生物传感器
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高影响因子
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