MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·15
·
IF 5.3
Zhang Yan,Yan Yuting,Huang Yue
An innovative electrochemical biosensor is proposed for the sensitive detection of ofloxacin (OFL) by integrating exonuclease I (Exo I)–assisted recognition-transduction strategy into the MXene/AuNP-enhanced electrochemical platform. Through ingenious design, the presence of OFL triggers the competitive displacement reaction and enables Exo I to hydrolyze the released single-stranded DNA probe containing the sequence of Pb 2+ -DNAzyme, protecting the signal probes on the electrode surface from DNAzyme cleavage, which eventually brings about a remarkable electrochemical signal positively related to the concentration of OFL. MXene/AuNPs with excellent conductivity, large specific surface area, and good chemical stability are employed as the electrode substrate to efficiently accelerate charge transfer and increase the loading capacity for signal probes, leading to the enhancement of signal output and endowing the biosensor with high sensitivity, rapid response, and fine robustness. Taking advantage of OFL recognition–initiated Exo I hydrolysis to DNAzyme, the electrochemical platform with “signal-off/on” mode can detect OFL with good specificity and reduced background interference. Benefiting from the synergistic effect of the involved sensing elements, the developed electrochemical biosensor achieves a low detection limit of 0.47 pg mL −1 with a broad linear range and exhibits satisfactory detection performance in real food samples, indicating potential practical applicability in food safety monitoring. Graphical
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Environmental Technology & Innovation
·
2023
·
IF 7.1
Xiangyu Meng,Zhenran Wang,Kerui Li,Yiqing Liu,Dandan Zhao,Yongsheng Fu
In recent years, the extensive utilization of ofloxacin (OFX) has led to elevated concentration of OFX compound in the aquatic environment. At the same time, the inherent recalcitrance of OFX has presented a formidable challenge for OFX degradation. In this study, lanthanum cerium perovskite (LaCeO 3 ) was prepared by citric acid sol–gel method to activate bisulfite (BS) for the effective degradation of OFX. The results revealed that the LaCeO 3 /BS system removed 87.5% of OFX within 180 min with a pseudo-first-order kinetic constant of 3 × 10 −3 min −1 . Radical quenching experiments and electron paramagnetic resonance (EPR) spectroscopy confirmed that sulfate radicals (SO 4 • _ ) and hydroxyl radicals (HO • ) were the primary reactive species responsible for OFX removal in LaCeO 3 /BS system. The reaction mechanism indicated that Ce in LaCeO 3 /BS system served as the primary active site for BS activation, and a regenerating cycle involving ≡ Ce(III)/ ≡ Ce(IV) was present. Additionally, possible intermediate products were identified by a liquid chromatograph-mass spectrometer (LC-MS), revealing potential degradation pathways of OFX. Cycle tests and energy dispersive spectrometer (EDS) data demonstrated the favorable reusability and stability of LaCeO 3 .
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2026
·
IF 9.2
Wei Gao,Junzhuang Wu,Ting Guan,Imran Ali,Guoxin Tang,Yujiao Yue,Wei Li
The widespread use of antibiotics, particularly ofloxacin (OFL), has led to their persistent presence in aquatic environments. The acclimation of Tetradesmus obliquus ( T. obliquus ) using OFL was found to enhance both its tolerance and degradation capability. After 14 d of exposure to 1 mg/L OFL, the degradation rate of the acclimated T. obliquus was 25.81% higher than the non-acclimated, mainly due to enhanced bioaccumulation and biodegradation. Physiological and biochemical analyses showed that acclimated T. obliquus experienced significantly less growth inhibition and oxidative stress under OFL exposure than non-acclimated. Transcriptome results show that OFL exposure impacts DNA replication and repair, photosynthesis, membranes, oxidoreductases, and energy metabolism in both non-acclimated and acclimated T. obliquus . Acclimated T. obliquus by adjusting carbon metabolism to boost energy, enhancing antioxidant enzymes, upregulating ABC transporters to expel antibiotics, increasing carotenoid synthesis for photosynthetic protection, and using amino acid synthesis to offset reduced protein production. These adaptations greatly improve the survival and degradation abilities of algae that were acclimated through repeated OFL exposure. Molecular docking and dynamics simulations suggested the possible formation of new complexes between SOD and GST during acclimation. Overall, this study provides valuable insights into the adaptation of algae to antibiotic pollution and highlights the potential of acclimated microalgae for advanced bioremediation strategies.
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WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 5
·1-15
·
IF 2.9
Zheng Chao,Wu Junyou,Zhang Yujie,Yang Qin,Yang Yingchun
In this study, the walnut shell (WS) supported CoFe 2 O 4 nanopowder material (CoFe 2 O 4 /WS) were synthesized and characterized by X-ray diffraction, scanning electron microscopy, fourier transform infrared spectrometer, transmission electron microscope and X-ray photoelectron spectroscopy. The CoFe 2 O 4 /WS exhibited excellent catalytic activity in the process of activating peroxymonosulfate (PMS) to degrade ofloxacin (OFL). The results showed that 92.8% of OFL removal efficiency and 76.1% of mineralization rate were achieved at 1 g L –1 CoFe 2 O 4 /WS, 1 g L –1 PMS within 30 min. Moreover, the results of coexisting anions and organic matter indicated that the CoFe 2 O 4 /WS/PMS system has a certain anti-interference ability in complex environment. Furthermore, radical identification experiment demonstrated that SO 4 ·− was the dominant active substance during the OFL degradation process, and a possible activation mechanism of PMS by CoFe 2 O 4 /WS was proposed. Finally, possible degradation pathways of OFL were inferred based on the reaction intermediates detected by high-performance liquid chromatography-mass spectrometry. These findings will enrich the understanding of sulfate radical-advanced oxidation process (SR-AOPs) for antibiotic wastewater treatment and will guide the development of metal-organic frameworks as catalysts for PMS.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2025
·
IF 8.4
Yang Yu,Na Jiang,Yuanbo Zhou,Fei Huang,Yide He,Yongjun Zhang
Ofloxacin (OFX), commonly employed in the treatment of infectious diseases, is frequently detected in aquatic environments and poses potential ecological risks. UV/H 2 O 2 oxidation has been recognized as an efficient approach for removing antibiotics. In this study, Cu-doped waste-tire carbon was prepared and used as a UV/H 2 O 2 catalyst for the degradation of OFX. The results showed that the OFX removal was 89.3% within 90 min under the optimal reaction conditions. It was found that ZnO, used in the tire manufacturing to promote rubber vulcanization for enhanced stability and durability, played an important role in UV/H₂O₂ oxidation for OFX degradation. Toxicity experiments conducted with a microbial degradation respirometer demonstrated that the treated water exhibited low toxicity. This study introduces a sustainable catalyst derived from waste tires, and facilitating the development of metal-carbon catalysts for the effective removal of antibiotics from wastewater.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-9
·
IF 5.705
Zhou Xiaotong,Wang Lumei,Shen Guoqing,Zhang Dongwei,Xie Jialun,Mamut Abdureyim,Huang Weiwen,Zhou Shanshan
A colorimetric method is presented for the determination of the antibiotic ofloxacin (OFL) in aqueous solution. It is based on the use of an aptamer and gold nanoparticles (AuNPs). In the absence of OFL, the AuNPs are wrapped by the aptamer and maintain dispersed even at the high NaCl concentrations. The solution with colloidally dispersed AuNPs remains red and has an absorption peak at 520 nm. In the presence of OFL, it will bind to the aptamer which is then released from the AuNPs. Hence, AuNPs will aggregate in the salt solution, and color gradually turns to blue, with a new absorption peak at 650 nm. This convenient and specific colorimetric assay for OFL has a linear response in the 20 to 400 nM OFL concentration range and a 3.4 nM detection limit. The method has a large application potential for OFL detection in environmental and biological samples.
Schematic of a sensitive and simple colorimetric aptasensor for ofloxacin (OFL) detection in tap water and synthesic urine. The assay is based on the salt-induced aggregation of gold nanoparticles which results in a color change from red to purple.
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Frontiers in Environmental Science
·
2024
·
IF 3.3
Fangfang Li,Qiuling Yan,Zhongwen Li,Zhicheng Tan,Yuxuan Li,Siyao Wang,Jiawen Guo,Hongbo Peng,Lin Wang
Introduction: Soil organic matter plays an important role in the long-term “locking” of organic contaminants in soil environment. Recently, microbial-derived organic matter have been recognized as essential components of stabilized soil carbon pools. However, the contribution of microbial-derived organic matter to sorption of organic contaminants remains unclear.Methods: Here, we obtained microbial-derived organic matter-mineral composites by inoculating model soil (a mixture of hematite and quartz sand (FQ) or montmorillonite and quartz sand (MQ)) with natural soil microorganisms and different substrate-carbon (glycine (G), glucose (P), or 2, 6-Dimethoxyphenol (B)), which were named GF, PF, BF, GM, BM, and PM, respectively. Batch sorption/desorption experiments were conducted for phenanthrene (PHE) and ofloxacin (OFL) on the composites.Results and Discussion: The composites cultured with 2,6-dimethoxyphenol had the highest carbon content (0.98% on FQ and 2.11% on MQ) of the three carbon substrates. The carbon content of the composites incubated with MQ (0.64%–2.11%) was higher than that with FQ (0.24%–0.98%), indicating that montmorillonite facilitated the accumulation of microbial-derived organic matter owing to its large specific surface area. The sorption of PHE by microbial-derived organic matter was mainly dominated by hydrophobic partitioning and π-π conjugation, whereas the sorption of OFL was mainly dominated by hydrophobic hydrogen bonding and π-π conjugation. The sorption of OFL onto the composites was more stable than that of PHE. Microbial-derived organic matter -mineral composites can reduce the risk of organic contaminant migration in soil, particularly ionic organic contaminants.
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JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
·
2026
·Vol. 40
·期 4
·e70788
·
IF 3.6
Ran Yu,Rong Shen,Liang Chen,Peng Li
The hydrolysis of proteins by pepsin is of great significance for the biological utilization of proteins and the discovery of functional peptide molecules. Bovine serum albumin (BSA) and bovine collagen I (BCI) are both commonly used natural source proteins for studying the hydrolysis characteristics of pepsin. UHPLC − MS/MS, peptidomics, and molecular docking technologies were employed to investigate the underlying mechanism responsible for the inhibitory effect of ofloxacin on pepsin. The molecular weight distribution of peptides produced by pepsin in this study was mostly in the range of 600 Da to 1800 Da, and peptide segments were mostly composed of 9−11 amino acids. The predominant terminal amino acids were proline, glycine, leucine, valine, serine, and threonine. Ofloxacin led to conformational changes of the hydrolysis active sites of pepsin by forming hydrogen bonds with aspartic acids. When the key aspartic acid residues in the active center of pepsin were inhibited, the numbers of peptides TPAQD, VSVDAA, TVLFD, and TVIFD were upregulated. The hydrolysis characteristics of pepsin were changed, shown as an increase in the proportion of low molecular weight peptides and a decrease in the hydrophobicity of peptide segments in the hydrolysates. The study contributed to the evaluation of the activity of peptides from homologous protein hydrolysis by pepsin and the elucidation of the inhibitory mechanism of ofloxacin on pepsin.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Weilu Yang,Nihal Oturan,Jialin Liang,Mehmet A. Oturan
This study aimed at exploring the performance of the electro-Fenton (EF) process with a boron-doped diamond (BDD) anode and an electrochemically exfoliated graphene-based cathode (EEGr), for removal of antibiotic ofloxacin (OFLO) from contaminated water. Compared with anodic oxidation (AO) process with BDD anode, the EF process with BDD anode (EF-BDD) could improve the decay kinetics of OFLO by 1.4–2.6 times and reduce the disposal cost by 28 %–41 %. Removal efficiency of TOC in EF-BDD process under 4.2 mA cm −2 was better than in AO process under 8.3 mA cm −2 , pointing out that the cost effectiveness of the EF process for mineralization of OFLO under low current density conditions. This advantage is due to the simultaneous formation of homogeneous ( • OH) and heterogeneous (BDD( • OH)) hydroxyl radicals in the process. The synergistic factor of • OH and BDD( • OH) for mineralization of OFLO decreased with the current density rising from 4.2 (0.28) to 16.6 mA cm −2 (-0.08). Decay kinetics of OFLO in the EF-BDD and AO were more converging under higher current densities owing to the production of large amount of BDD( • OH). In the EF-BDD, a trade-off should be considered between accumulated BDD( • OH) on the surface of BDD anode and H 2 O 2 generation on the cathode. Absolute rate constant for oxidation of OFLO by • OH ( k OFLO ) was calculated to be 3.86 × 10 9 M −1 s −1 . Evolution of carboxylic acids and inorganic ions as well as toxicity assessment were followed during the treatment of OFLO solutions. Results obtained proved that the EF-BDD process is outstanding for oxidative degradation of OFLO thanks to the synergistic effect of •OH and BDD( • OH). These very strong oxidants being non-selective, this result can be extended to the treatment of antibiotic organic pollutants.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Minjun Ma,Yu Liu,Honghong Chang,Yingxiong Wang,Yan Qiao
Ofloxacin is one of the most potent antibiotics. But its low solubility in water has limited its use in site targeted delivery and topical applications. This study investigates the PAMAM-NH 2 (amine-terminated polyamidoamine dendrimers) delivery of ofloxacin in water based on host-guest chemistry and reveals the interaction mechanism of PAMAM-NH 2 with ofloxacin by utilizing NMR techniques including 1D chemical shift titration, 2D 1 H– 13 C HSQC titration, NOESY technique, STDD method, and 2D DOSY analysis. Ofloxacin interacts with surface amine groups of PAMAM-NH 2 via ionic interactions, and ofloxacin is also encapsulated into the PAMAM interior cave through hydrophobic interactions. An overall mechanism was proposed for the PAMAM-NH 2 adsorption of ofloxacin, and then the binding affinity was determined. The experimental data demonstrate the potential of the PAMAM-NH 2 for the drug molecules delivery.
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Molecular Catalysis
·
2019
·
IF 2.938
Juan Liu,Xia Wu,Jingjing Liu,Conglu Zhang,Qi Hu,Xiaohong Hou
In this work, ofloxacin degradation was investigated by using Fe 3 O 4 -CeO 2 /activated carbon (AC) as heterogeneous Fenton catalyst. Response surface methodology based on central composite design (CCD) was used to investigate individual and interactive effects of three parameters on the ofloxacin degradation, i.e., solution pH, H 2 O 2 dosage and initial ofloxacin concentration. Optimal conditions were determined to be pH 3.3, H 2 O 2 20.0 mM, ofloxacin 12.0 mg L −1 and catalyst 0.5 g·L −1 . The ofloxacin degradation efficiency and the total organic carbon (TOC) removal was 95% and 54% within 60 min, respectively. The obtained results agreed well with the modeling prediction ( R 2 = 0.9826, and Adj- R 2 = 0.9783). The kinetic analysis implied that the reaction followed first-order kinetic with R 2 = 0.9965. According to mass spectrometry qualitative analysis, eight intermediates of ofloxacin were detected and two possible degradation pathways were proposed. The catalyst still showed degradation efficiency of 82.4% toward ofloxacin after five runs.
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TALANTA
·
2025
·
IF 6.1
Wenchao Ran,Chi Xu,Xiaomei Pan,Xiaojun Huang,Chong Wang,Xianggui Chen,Jie Tang,Imran Mahmood Khan,Yukun Huang
Ofloxacin, a synthetic antimicrobial drug, exhibits significant therapeutic potential in combating various bacterial infections. The exploration of ofloxacin's antimicrobial properties and the unique catalytic activity of G-triplex(G3) DNAzymes demonstrate the continuous efforts to develop cutting-edge biosensors for bacterial detection. G3 outperforms G4 due to a short, easily generated and dissociated sequence, which allows G3 multimeric sequences to fold more accurately than G4 ones of equivalent length, resulting in higher activity for the formed G3/hemin. This study constructed a novel colorimetric DNAzyme biosensor based on G3/hemin for ofloxacin in food. Aptamer-functionalized Fe 3 O 4 magnetic nanoparticles acted as magnetic separation and detection probes, while G3/hemin served as DNAzyme. Under the optimum condition, the presence of ofloxacin initiated the catalytic oxidation to yield a colored product to quantify the ofloxacin concentration with the linear range of 50–300 nmol/L. Under the optimized conditions, a limit of detection of 15.25 nmol/L was obtained by directly detecting the fluorescent signals, which shows obvious superiority over the previously reported methods. This method offers the advantages of simplicity, rapid detection, and high recovery rates in standardization experiments involving actual samples such as beef jerky and ham sausage, paving the way for its widespread implementation in the fields of biomedical diagnostics, environmental monitoring, and food safety.
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