BIOORGANIC CHEMISTRY
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2025
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IF 4.7
Wenlan Li,Guoping Qiu,Manjun Kang,Can Zhang,Wei Quan,Hui Zhou,Xiankang Fan,Jie Luo,Baiyi Lu,Maiquan Li
Obesity is consistently associated with dysregulated glycolipid metabolism. The regulation of α-amylase and pancreatic lipase plays a pivotal role in maintaining glucose and lipid homeostasis. In this study, we demonstrated that Osmanthus fragrans flower phenylethanoid glycoside-rich extract (OFE) effectively suppressed α-amylase activity through mixed-type inhibition and pancreatic lipase activity via competitive inhibition. Molecular docking analysis revealed that acteoside and salidroside bond to the amino acid residues of these enzymes primarily through hydrogen bonding and hydrophobic effect. Further investigation using UV–vis and fluorescence spectroscopy indicated that acteoside interacted with α-amylase via static quenching and with pancreatic lipase through dynamic quenching. Interestingly, while acteoside quenched the fluorescence of both enzymes, salidroside enhanced their fluorescence. Circular dichroism spectroscopy analysis showed distinct structural effects: acteoside increased α-helix and β-sheet content while reducing β-turn and random coil structures, whereas salidroside elevated α-helix and β-sheet content but decreased β-turn and random coil formation. Our findings demonstrate that acteoside and salidroside are effective natural inhibitors of α-amylase and pancreatic lipase, supporting the potential of Osmanthus fragrans flower as a dietary intervention for glycolipid metabolism disorders.
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FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Xiangrui Ren,Temirlan Khamiddolov,Li Niu,Qian Lin,Wenqing Zhao,Yìxiang Liu,Wenhao Li
This study systematically evaluated the effects of ultrasound-assisted vacuum freeze-drying (Ul-FD), variable-temperature and variable-speed hot air drying (VT/VS-HD), and modulated microwave drying (Mo-MD) on the quality of Osmanthus fragrans var. thunbergii ( O. fragrans ). The results indicated that Ul-FD best preserved chromatic attributes and cellular structure, retaining the highest levels of total phenolics (1.08 μg GAE/mg DW), total flavonoids (0.80 μg RE/mg DW), and antioxidant capacity (DPPH scavenging activity of 94.57%), significantly outperforming the other treatments. Mo-MD significantly reduced the drying time to 20 min and exhibited the fastest dynamic process of moisture migration and bound water transformation. HRLC-MS identified 82 phenolic compounds, with Ul-FD best preserving polyphenol diversity, Mo-MD enriching heat-resistant aglycones, and VT/VS-HD promoting the formation of small-molecular acids. PCA and PLS-DA identified 17 differential metabolites, demonstrating that drying methods significantly reshape the polyphenome. KEGG enrichment analysis indicated that the phenylpropanoid and flavonoid biosynthesis pathways were the main responsive routes. This study links the drying process to phenolic metabolism and bioactive quality. It identifies Ul-FD for high-value, Mo-MD for industrial, and VT/VS-HD for general applications, providing a basis for optimizing O. fragrans drying and product development.
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Food Chemistry-X
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2026
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IF 9.2
Weiyi Xu,Yutong Meng,Fan Yu,Huanfeng Ye,Xuejiao Xu,Jie Chen,Xianrui Liang,Sheng Fang
This study evaluated Osmanthus fragrans fruit (OFF) peel as a potential source of anthocyanin-based natural colorants. Anthocyanins were extracted using acidified ethanol and purified by macroporous resin adsorption, yielding a pigment-rich extract with a recovery of 10.4 ± 0.2% from dried peel. HPLC–MS/MS analysis indicated that cyanidin-3-O-rutinoside (C3R) was the predominant anthocyanin in the extract. The molar absorptivity of C3R was experimentally determined to enable more accurate quantification of C3R-rich systems. The purified extract exhibited typical pH-dependent color behavior, maintaining a stable red coloration under acidic conditions (pH 2.0–4.0). Thermal stability experiments showed that C3R degradation was strongly influenced by temperature and pH, and the degradation behavior followed first-order kinetics. In addition, the extract displayed measurable antioxidant capacity in DPPH and FRAP assays. These results demonstrate that O. fragrans fruit peel is a promising source of C3R-rich anthocyanins with potential applications as natural colorants in acidic food systems.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2015
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IF 2.912
Yirong Jiang,Shuqin Mao,Weisu Huang,Baiyi Lu,Zengxuan Cai,Fei Zhou,Maiquan Li,Tiantian Lou,Yajing Zhao
Variations of phenylethanoid glycoside profiles and antioxidant activities in Osmanthus fragrans flowers through the digestive tract were evaluated by a simulated digestion model and UPLC/PDA/MS. Major phenylethanoid glycosides and phenolic acids, namely, salidroside, acteoside, isoacteoside, chlorogenic acid, and caffeic acid, were identified in four cultivars of O. fragrans flowers, and the concentration of acteoside was the highest, being up to 71.79 mg/g dry weight. After simulated digestion, total phenylethanoid glycoside contents and antioxidant activities were significantly decreased. Acteoside was identified as decomposing into caffeic acid, whereas salidroside was found to be stable during simulated digestion. According to Pearson’s correlation analysis, acteoside contents showed good correlations with antioxidant activities during simulated digestion (R2 = 0.994, P < 0.01). In conclusion, acteoside was the major contributor to the antioxidant activity of O. fragrans flowers, and salidroside was considered as the major antioxidant compound of O. fragrans flowers in vivo.
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INDUSTRIAL CROPS AND PRODUCTS
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2024
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IF 5.6
Jun-Hui Du,Dong-Mei Yang,Tong-Tong Guan,Jie Wang,Yi-Long Ma,Zhao-Jun Wei
Osmanthus fragrans seeds (OFSs) abundant in protein are valuable resources of bioactive peptides. To elucidate the potential hypoglycemic peptides released during the digestion, OFSs protein was extracted and digested in vitro . The effects of the digestion step and molecular weight on the α-glucosidase inhibitory ability of the digested protein were evaluated, and the mechanism and structure of the main effective peptides in the digesta were studied. The results indicated that the digesta after the small intestine digestion displayed higher inhibition (58.5 %), from which the fraction below 3 kDa was more effective (67.34 %). Among the seven sub-fractions (F1∼F7) below 3 kDa, F1 obtained the highest α-glucosidase inhibitory ability (60.69 %). Three peptides (ERFQ, VHHK, and EKFMKE) were identified from F1, and their efficacy was verified by corresponding synthetic peptides, showing the α-glucosidase inhibitory rates of 79.1 % ± 2.24 %, 65.67 % ± 1.97 % and 61.94 % ± 1.97 %, respectively. According to the molecular docking, these peptides inhibited the α-glucosidase by forming secondary forces (e.g. hydrophobic interaction) with the key amino acids (e.g. D327, D443, Y299, and F575) of the enzyme, with docking scores ranging from −151.038 to −171.907. This study demonstrated OFSs could release α-glucosidase inhibitory peptides during digestion and may serve as functional ingredients to control diabetes.
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Food Frontiers
·
2025
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IF 6.9
Qinle Huang,Zhangtie Wang,Binhai Shi,Guoliang Jie,Songbai Liu,Fuli Nie,Fan Wang,Zhenjiang Zhou,Siyu Chen,Jianfu Shen,Baiyi Lu
Osmanthus fragrans extract (OFE) has significant potential for application in the beverage and cosmetic industries. However, the conventional extraction processes of OFE are affected by multiple factors, making it challenging to identify parameters that can be synergistically optimized for diverse qualities. This study aimed to establish a multi-objective optimization (MOO) method for the ultrasonic-assisted extraction of O. fragrans var. thunbergii. The effects of time, temperature, ultrasonic power, and solid-liquid ratio on yield, content of total phenylethanol glycosides, verbascoside and salidroside, and colorimetric parameters were compared using response surface methodology and machine learning algorithms. Multi-layer perceptron, XGBoost, and support vector regression were evaluated as base models for constructing an ensemble model, using the SHAP algorithm for interpretation. A MOO method based on BP-ANN and NSGA-II was developed to maximize the content of bioactive compounds in the extract while considering other quality indicators. The optimal conditions for the target quality attributes were determined to be a temperature of 54°C, an extraction time of 52 min, a solid-liquid ratio of 16 mL/g, and an ultrasonic power of 345 W. This study provides a novel approach that combines artificial intelligence and MOO for process optimization in the plant extraction industry.
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Journal of Applied Research on Medicinal and Aromatic Plants
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2021
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IF 3.4
Weizhuo Tang,Bin Wang,Miaomiao Wang,Mengmeng Wang
In this work, optimization of ultrasound-assisted extraction (UAE) of Osmanthus fragrans (OF) fruit oils from four representative species of OF groups was firstly performed. A Box-Behnken design was applied to investigate and optimize the process variables. Among them, the specie of OF var. latifolius Makino (OFL) had the highest oil yield (18.53 %) by UAE under the optimal conditions with an ultrasound extraction time of 33 min, ultrasound temperature of 52 ℃ and liquid-to-solid ratio of 12 mL/g. Furthermore, the fatty acid composition, physicochemical characteristics, and antioxidant activity of OFL fruit oil extracts obtained through UAE and Soxhelet extraction (SE) were determined and compared. The results demonstrated that the oil yield and fatty acid composition between UAE and SE were similar, while the oil obtained by UAE displayed superior physicochemical properties and antioxidant activity . These findings suggest that UAE represents an effective method for OF fruit oil extraction.
研究领域:
食品科学
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天然产物化学
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油脂化学
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抗氧化研究
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提取工艺优化
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FOOD CHEMISTRY
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2020
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IF 6.306
Fangli Hu,Xiaoyan Liao,Yanli Guo,Satoshi Yamaki,Xiaodong Li,Naoki Hamada,Yuki Hashi,Zilin Chen
Triterpenic acids possess rich biological activity. Due to slight differences in structure and polarity, the simultaneous determination of isomeric triterpenic acids is challenging. In the present work, a simple and effective approach to chromatographic separation of such compounds based on conventional C18 stationary phase with gradient elution was developed, which allowed the simultaneous separation of eleven analytes including euscaphic, arjunic, tormentic, arjunolic, asiatic, pomolic, maslinic, corosolic, oleanolic, ursolic and 2- Epi tormentic acid (internal standard). This approach with mass spectrometric detection and ultrasonic extraction was fast, sensitive and accurate for analyzing isomeric triterpenic acids in O. fragrans fruits with a toal duration of the analytical cycle (including pretreatment) within one hour. The LODs lie in ranges of 0.8–12 ng/mL (30 ng/mL for asiatic and corosolic acid). The developed method was validated and successfully applied in ten batches of O. fragrans fruits, which could reflect the detail content difference of triterpenic acid components.
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Arabian Journal of Chemistry
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2021
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IF 5.165
Weizhuo Tang,Shuang Li,Miaomiao Wang,Bin Wang
In this study, simultaneous optimization of ultrasound-assistant extraction (UAE) of four representative groups of Osmanthus fragrans (OF) fruit was firstly performed using the response surface methodology (RSM). Subsequently, qualitative and quantitative analysis of irioid glycosides from the optimized OF fruit extract was performed using UPLC-ESI-Orbitrap-MS/MS. A total of 13 irioid glycosides were tentatively identified, of which six compounds were identified for the first time. Finally, the anti-inflammatory activity of the optimized OF var. aurantiacus Makino (OFA) fruit extract was evaluated based on a CuSO 4 -induced zebra fish model. The biological results demonstrated that OFA fruit extract at a concentration of 5 μg/mL could significantly reduce the number of macrophages, but no significant activities were observed at high concentrations (10 and 50 μg/mL). These results suggest that OF fruit has the promise as a functional additive in food for health promotion.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2022
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IF 1.193
Ting-Ting ZHANG,Xiao-Yan LIAO,Jing REN,Chen CHEN,Yu-Ying WAN,Fang WANG,Zi-Lin CHEN
Neonuezhenide, nuezhenide, acteoside, isoacteoside and salidroside are common phenylethanoid glycosides in Osmanthus fragrans ( O. fragrans ) fruits and have various physiological and pharmacological activities. The electrochemical determination of the five components in O. fragrans fruits was researched at electrochemically reduced graphene oxide film modified glassy carbon electrode (ERGO/GCE). ERGO film was prepared by one-step deposition and showed a honeycombed network structure and provided good conductivity, controllability and stability. Compared with bare GCE or chemically reduced graphene oxide film modified electrode (RGO/GCE), the voltammetric behaviours of all five active components were improved at ERGO/GCE in terms of peak current and potential due to excellent electrical properties of ERGO. The numbers of protons and electrons transferred in electrochemical redox reaction were calculated and the possible electrode processes of five components have been estimated herein. The number of electrons involved was equal to that of protons transferred in the electrode processes for each compound and it was 2 for neonuezhenide, 1 for nuezhenide, 4 for acteoside, 4 for isoacteoside, and 1 for salidroside, respectively. Finally, a simple electrochemical method was developed for determination of the five components. This method has wide linear range with low detection limit of 3 μM for neonuezhenide, 6 μM for nuezhenide, 4 μM for acteoside, 3 μM for isoacteoside, and 3 μM for salidroside, respectively. Moreover, this method could be coupled with high performance liquid chromatography (HPLC) and applied to determination of the five active components in extractive from O. fragrans fruits. Compared with high performance liquid chromatography-mass spectrometry (HPLC-MS), this method would be competitive and alternative for the analysis of active components in real samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Baolei Fan,Tingting Li,Xiaofang Song,Chao Wu,Chunqi Qian
A specific HPLC-MS/MS (High-Performance Liquid Chromatography with tandem Mass Spectrometry) method was developed and validated for simultaneous determination of several monosaccharides in three kinds Osmanthus fragrans Lour . After extraction, separation, protein removal, pigment removal and hydrolysis , monosaccharides was finally obtained from Osmanthus fragrans Lour . Positive ion mode detection and Multiple Reaction Monitoring (MRM) mode were used for quantitative analysis by PMP pre-column derivatization and Electrospray Ionization (ESI). Analysis and content determination of 6 monosaccharide components in 3 kinds of Osmanthus fragrans Lour . The HPLC separation was achieved on a Shim-pack VP-ODS6022748 (150 L × 2.0) with gradient elution at a flow rate of 0.2 ml/min in a run time of 40 min, and the mobile phase was acetonitrile-5 mmol/L ammonium acetate. PMP derivatization in HPLC-MS/MS can accurately measure Osmanthus fragrans Lour . mannose (Man), ribose (Rib), rhamnose (Rha), galacturonic acid (Gal UA), glucose (Glu), galactose (Gal), xylose (Xyl), fucose (Fuc). The results showed that HPLC-MS/MS pre-column derivatization method was simple and rapid, with small measurement error, but high sensitivity and good repeatability. The analysis of monosaccharide components in polysaccharide components has important practical significance.
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SCIENTIA HORTICULTURAE
·
2025
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IF 4.2
Yingyu Huang,Hao Wei,Ya Chen,Xiulian Yang,Lianggui Wang,Yuanzheng Yue
Osmanthus fragrans is valued as an ornamental tree appreciated for its fragrance, in which β -ionone is one of the most critical volatile organic compounds (VOCs). The distribution of O. fragrans is mainly limited by low temperature (LT), which in other species can also induce the synthesis of endogenous ABA and the formation of floral aroma. However, the regulatory relationship between LT and ABA on the floral aroma quality in O. fragrans remains unclear. In this study, flowers of O. fragrans ‘Liuye Yingui’ were treated with LT, ABA, or LT+ABA. Metabolic analysis of VOCs and PCA indicated that all three treatments produced clusters well separated from the control group. However, while both of ABA and LT+ABA treatments decreased the content of β -ionone, the LT treatment alone caused an increase. Notably, a significant enrichment in the carotenoid biosynthesis pathway, which is closely associated with β -ionone synthesis was observed in the control vs. ABA and LT vs. LT+ABA groups, indicating that the ABA affects β -ionone contents mainly through its effects on the carotenoid biosynthesis pathway. By conducting an analysis of the weighted gene co-expression network, a module of genes most positively correlated with β -ionone was identified, in which one hub structural gene OfPDS3–2 , as well as three transcriptional factors OfMYB187, OfATHB15 , and OfTCP3 were indicated to have potentially important regulatory roles in β -ionone synthesis under LT and ABA stimuli. Together, these findings indicate that the promotion of β -ionone synthesis by LT can be negatively regulated by exogenous ABA and suggest useful genetic resources for the floral aroma improvement breeding in O. fragrans .
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