ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Li Lu,Qiwei Chai,Shanying He,Chunping Yang,Dong Zhang
Plant-endophyte synergism has been demonstrated to play a key role in the phytoremediation of contaminated water and soil. Phytoalexins, a type of chemical component in the plant apoplast, can be produced by plants in response to stimulation by endophytes. Phytoalexins may have distinct effects on the nutritional and metabolic functions of endophytes; however, direct evidence is not available to prove the effect of phytoalexins on the hydrophobic organic contaminants (HOC)-degradation activity of endophytes. In this paper, three different types of phytoalexins, coumarin, resveratrol and rutin, were selected to study their effect on the removal of polycyclic aromatic hydrocarbons (PAHs) by an endophytic bacterium Methylobacterium extorquens C1. The effects of the three phytoalexins on bacterial sorption and intracellular enzymatic activities were tested to further analyze the mechanism by which the phytoalexins affect the PAH degradation performance of M. extorquens C1. The results showed that the removal rate of PAHs by M. extorquens C1 increased in the presence of low levels of the three phytoalexins. The most effective concentrations of coumarin, resveratrol and rutin were 0.20, 0.15, and 0.25 mg/L, respectively, and the removal rate of PAHs was increased by approximately 18.3–35.0%. At the optimal concentrations, the three phytoalexins significantly promoted the sorption of PAHs by M. extorquens C1, and also enhanced the activities of catechol dioxygenases and dehydrogenase of M. extorquens C1. The positive effect of phytoalexins on both bacterial sorption and intracellular enzymatic activities promotes the overall removal of PAHs from endophytes. These results may deepen our understanding of plant-microbe cooperative mechanisms in the degradation of organic pollutants and provide a new approach for chemically enhanced bioremediation in the future.
研究领域:
环境科学
·
微生物学
·
植物科学
·
生物修复
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2018
·
IF 6.653
Songqiang Deng,Tan Ke,Yanfang Wu,Chao Zhang,Zhiquan Hu,Hongmei Yin,Limin Guo,Lanzhou Chen,Dayi Zhang
Polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) are predominant pollutants normally coexisting at electronic waste dumping sites or in agricultural soils irrigated with wastewater. The accumulation of PAHs and HMs in food crops has become a major concern for food security. This study explored the hydroponic uptake of 16 priority PAHs and 5 HMs (Cd, Cr, Cu, Pb, and Zn) by pak choi (Brassica chinensis L.). PAHs exhibited stronger inhibition on pak choi growth and physiological features than HMs. Five HMs were categorized into high-impact HMs (Cr, Cu, and Pb) and low-impact HMs (Cd and Zn) with distinct behavior under the coexposure with PAHs, and low-impact HMs showed synergistic toxicity effects with PAHs. Coexposure to PAHs and HMs slightly decreased the uptake and translocation of PAHs by pak choi, possibly attributing to the commutative hindering effects on root adsorption or cation−π interactions. The bioconcentration factors in PAHs + HMs treatments were independent of the octanol–water partition coefficient (Kow), owing to the cation-π interaction associated change of Kow and induced defective root system. This study provides new insights into the mechanisms and influential factors of PAHs uptake in Brassica chinensis L. and gives clues for reassessing the environmental risks of PAHs in food crops.
研究领域:
环境科学
·
农业科学
·
毒理学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2017
·
IF 6.065
Shao-Lin Xie,Muhammad Junaid,Wan-Ping Bian,Juan-Juan Luo,Jabir Hussain Syed,Chao Wang,Wen-Xu Xiong,Yan-Bo Ma,Aping Niu,Xiao-Jun Yang,Ji-Xing Zou,De-Sheng Pei
The polycyclic aromatic hydrocarbons (PAHs) and 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) are classified as human carcinogens, and can also cause serious health problems. To develop a convenient bio-monitoring tool for the detection of PAHs and TCDD in the environment, we generated a transgenic zebrafish line Tg(cyp1a:mCherry) with cyp1a promoter driving mCherry expression. Here, Tg(cyp1a:mCherry) embryos were treated with different concentrations of TCDD and five US EPA priority PAHs congeners. The results showed that the expressions of mCherry and endogenous cyp1a were consistent with the PAHs exposure concentrations and were largely induced by TCDD and ≥4-ring PAHs. Moreover, the sensitivity of Tg(cyp1a:mCherry) embryos was also evaluated through monitoring of the PAHs contamination in the water and soil samples. The elevated red fluorescent signals and cyp1a expression levels were observed in Tg(cyp1a:mCherry) zebrafish after exposure to water samples and soil organic extracts with higher concentrations of ≥4-ring PAHs. These results further strengthen our findings of concentration- and congener-dependent response of the newly established zebrafish. Taken together, the newly established zebrafish line will prove as a sensitive, efficient and convenient tool for monitoring PAHs and TCDD contamination in the environment.
研究领域:
环境科学
·
毒理学
·
基因工程
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水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2011
·Vol. 136
·期 22
·4774-4779
·
IF 3.913
Di Pan,Chunyan Chen,Fei Yang,Yiming Long,Qingyun Cai,Shouzhuo Yao
Titanium wire-based solid phase extraction (SPE) is developed for the analysis of six different polycyclic aromatic hydrocarbons in water samples with coupling with high performance liquid chromatography. The adsorbent is a Ti wire on which TiO2 nanotubes are first formed by anodization and then Au nanoparticles and n-octadecanethiol are modified. The surface composition and structure of the adsorbent were characterized in detail using field emission scanning electron microscope and Fourier transform infrared spectroscopy. The main parameters affecting the extraction efficiency, such as the volume of the organic modifier, extraction time, elution solvent, and desorption time were optimized. Good linearities ranging from 0.025 to 30 μg L−1 with correlation coefficients (R2) ranging from 0.9964 to 0.9999 and low limits of detection were obtained in spiked water samples. Furthermore, this method was successfully applied in the analysis of real river water samples with recoveries in the range of 75.33–115.83% and relative standard deviations ranging from 4.85 to 10.77%. The developed SPE can be a particularly convenient means of sample pretreatment for environmental water samples.
研究领域:
环境科学
·
分析化学
·
水质分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yang Yang,Yifan Li,Qinhui Xie,Bo Jiang,Junbo Li,Yunfei Xie,Wei Ji
Biomimetic cytochrome P450 for chemical activation of environmental carcinogens is an efficient in vitro model for evaluating their mutagenicity and ultimately acquiring the metabolites that cannot be easily accessed by conventional routes of organic synthesis. Different kinds of mutagen derived from polyaromatic hydrocarbons (PAHs) by metalloporphyrin/oxidant model systems have been reported, but the underlying molecular mechanisms are poorly understood. Herein, we have for the first time demonstrated an effective surface-enhanced Raman scattering (SERS) protocol to study the dynamics and biomimetic metabolic behaviors of pyrene (Pyr) in the presence of various oxygen donors. Quantitative information on the relative concentration of Pyr and its metabolites in the biomimetic system can be extracted from the SERS spectra. On the basis of our results, we conclude that the oxidative metabolism of Pyr is highly influenced by the types and concentrations of oxygen donors, leading to the formation of 1-hydroxypyrene and dioxygenated products. Besides, the addition of an appropriate amount of an organic solvent can promote the formation of secondary oxidation products. These results offer valuable insights into the dynamics of PAHs metabolism and the regulation of their metabolic pathways in biomimetic activation. In comparison to traditional liquid chromatography–mass spectrometry, the present SERS approach is more suitable for high-throughput evaluation of the metabolic process and kinetics of PAHs. We anticipate that this approach will enable a more general and comprehensive tracking of metabolic dynamics and molecular mechanisms involved in the biomimetic activation of other xenobiotics, such as procarcinogens, promutagens, and drugs.
研究领域:
环境科学
·
分析化学
·
生物化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Lianghe Lv,Lina Sun,Chunli Yuan,Yue Han,Zhaohui Huang
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous persistent organic pollutants in soil, which have carcinogenic, teratogenic and mutagenic hazards. The effects of rhamnolipid (RL), nano zero-valent iron (nZVI), and anthraquinone-2,6-disulfonic acid (AQDS) on the degradation of PAHs in soil were studied. It was found that the treatment of 5 mg·kg −1 RL + 1% nZVI +0.2 mmol·kg −1 AQDS had the highest degradation rate. The degradation rate of total PAHs and HMW-PAHs was 72.81% and 79.47% respectively after 90 days. High-throughput sequencing showed that in RL + nZVI + AQDS enhanced soil, Clostridium , Geobacter , Anaeromyxobacter and Sphingomonas were the dominant species for anaerobic degradation of PAHs. Rhodococcus , Nocardioides , and Microvirga are the dominant species for aerobic degradation of PAHs. The activities of methyltransferase, dehydrogenase and catechol 1,2-dioxygenase in the anaerobic-aerobic degradation process of PAHs were consistent with the degradation process of PAHs, indicating the role of these enzymes in the degradation of PAHs. RL, nZVI, and AQDS combined enhanced microbial anaerobic-aerobic degradation has great application potential in remediation of PAHs-contaminated soil.
研究领域:
环境科学
·
土壤学
·
生物修复
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Xue Ma,Mengmeng Fu,Kai Hu,Li Gao,Chenchen Song,Shusheng Zhang,Renyong Zhao,Wenjie Zhao
Developing efficient and reliable adsorbents with simplified synthesis and mixed-mode performance is crucial for the accurate quantitative analysis of diverse coexisting environmental pollutants. A magnetic carboxylated hypercrosslinked organic polymer (Fe 3 O 4 /HCP-COOH) with reverse phase/cation exchange mixed-mode performance was synthesized through a two-stage process: (1) Fabrication of HCP-COOH via FeCl 3 -catalyzed Friedel-Crafts alkylation using 1,4-phenylenediacetic acid and 1,4-bis(chloromethyl)benzene; (2) In situ growth of Fe 3 O 4 nanoparticles on HCP-COOH through coprecipitation, utilizing FeCl₃ dually as polymerization catalyst and iron precursor to enhance process efficiency and waste minimization. The nanocomposite functioned as a mixed-mode sorbent for magnetic solid-phase extraction (MSPE), integrating dual retention mechanisms to selectively extract hydrophobic polycyclic aromatic hydrocarbons (PAHs) and cationic quaternary ammonium pesticides (QUATs), with subsequent analysis by GC-MS for PAHs and HPLC-MS/MS for QUATs respectively. Following optimization of adsorption pH, eluent composition, and contact time, the method exhibited high sensitivity with limits of detection (LODs) of 0.80-7.93 ng L⁻¹ for 15 PAHs and 0.02-16.31 ng L⁻¹ for 4 QUATs. Validation showed satisfactory recoveries of 89.5-98.3% (RSDs ≤11.2%) in snowmelt and 90.4-101.8% (RSDs ≤11.6%) in lake water (n=3), confirming practicality for simultaneous monitoring of hydrophobic and cationic contaminants in complex environmental water, offering significant advantages in multi-residue analysis for environmental monitoring applications.
研究领域:
环境化学
·
分析化学
·
材料科学
·
污染监测
·
可持续合成
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Processes & Impacts
·
2019
·Vol. 21
·期 7
·1184-1192
·
IF 2.688
Yaqin Gao,Yan Lyu,Xiang Li
The present study was conducted to examine the potential mechanisms responsible for the distribution of polycyclic aromatic hydrocarbons (PAHs) and their oxygenated derivatives (o-PAHs) in size fractionated particles and their environmental implications. Size-fractionated atmospheric particles were collected at urban Shanghai, China and analyzed for 5 PAHs and 6 o-PAHs. The concentrations of particle-bound ∑6o-PAHs (sum of 6 o-PAH congeners, 19.1 ± 4.5 ng m−3) were significantly higher than those of ∑5PAHs (sum of 5 PAHs, 9.5 ± 1.9 ng m−3). The size-fractionated distributions of airborne ∑5PAHs and ∑6o-PAHs displayed bimodal peaks at 0.7–1.1 and 9.0–10.0 μm. Emission sources and volatility were important factors influencing the size distribution of particle-bound 5 PAHs and 6 o-PAHs. The dry deposition fluxes of particle-bound 6 o-PAHs estimated from the measured data (3087 ± 467 ng m−2 d−1) were approximately 2 times higher than those of PAHs (1531 ± 344 ng m−2 d−1), with a total deposition flux of 4618 ± 743 ng m−2 d−1. The relative contributions of particles to dry deposition were also size-dependent, e.g., coarse (aerodynamic diameters (Dp) > 2.1 μm) particles dominated the dry deposition fluxes of PAHs and o-PAHs. These results highlight the significance of providing a comprehensive understanding of particle size distribution of PAHs and o-PAHs and their implication for dry deposition in China.
研究领域:
环境科学
·
大气化学
·
污染研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Hainan Lu,Wei Wang,Feng Li,Lizhong Zhu
The present study was conducted to explore the mechanisms of surfactant-enhanced phytoremediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs), focusing on the bioavailability of PAHs and microbial diversity. We investigated the remediation efficiencies of phenanthrene and pyrene after the addition of mixed surfactants (sodium dodecyl benzene sulfonate (SDBS) and Tween 80) of different ratios (1:1, 1:2, and 2:1) at the concentration of 100 mg/kg to soils cultured with ryegrass ( Lolium multiflorum L.). The fractions of phenanthrene and pyrene were determined using a sequential extraction method, and the microbial diversity was evaluated using 16S rRNA gene high-throughput sequencing. The results showed that mixed surfactants could enhance the remediation efficiencies of PAHs, and mainly occurred in the initial 21 days. Mixed surfactants at the ratio of 1:1 (HM1) showed the best remediation efficiency in enhancing the dissipation of pyrene in 21 days. Mixed surfactants showed little effects on the removal of phenanthrene. In general, HM1 significantly decreased the bioavailable, bound and residual fractions of pyrene; additionally, higher abundances of PAH-degradation bacteria and degradation-related genes were observed. Pearson correlation analysis among PAH degraders, degradation-related genes and bioavailable fraction of PAHs was performed. Our results indicated that mixed surfactants could promote the transformation of pyrene from the bound and residual fractions to bioavailable fractions and enhance the abundances of PAH degradation bacteria and PAH degradation-related genes, thereby enhancing the degradation of pyrene.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EXPERIMENTAL THERMAL AND FLUID SCIENCE
·
2024
·
IF 2.8
Xuan Zhao,Dandan Qi,Kaixuan Yang,Chen Chen,Lei Xu,Yaoyao Ying,Tianjiao Li,Guangxue Zhang,Dong Liu
The effects of four butanol isomers additions on sooting transition process were studied in iso -octane counterflow diffusion flame combining flame luminosity characteristics and gas-phase combustion chemistry. Flame luminosity characteristics was obtained to determine the sooting transition points of all investigated flames with homemade image processing and transition point extraction methods. The results indicated that the delay effect has occurred on sooting transition point in four blended flames with a little difference. Gas-phase combustion chemistry of the different flames in sooting transition process was studied experimentally by an online GC system as well as numerically by kinetic analysis realized with two distinct kinetic mechanisms. In addition, soot formation characteristics have also been qualitatively analyzed with soot model . Experiments and simulations show that, depending on the butanol isomer, the intermediate species production is clearly different. These different species pools play an important role on soot formation pathways as demonstrated by the simulations. Although n -butanol and sec -butanol were weaker in benzene formation, stronger C 2 H 2 production capacity was dominant in PAHs growth. Tert -butanol had the highest C 6 H 6 concentration with a ratio of 7 %−15 % higher than the other isomers due to the strongest IC 4 H 8 production capacity. Therefore, its flame also has the highest PAHs concentration. Both effect mechanisms existed in iso -butanol blended flame.
研究领域:
能源科学
·
化学工程
·
燃烧学
✓ 已验证
产品关联
DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2021
·
IF 7.033
Shiliang Wu,Jiajing Bao,Ziwei Wang,Huiyan Zhang,Rui Xiao
Catalytic etherification is a new and developing method for the upgradation of pyrolysis bio-oil into high performance bio-based long-chain ethers. In this work, the application of bio-based long-chain ether oxygenated additives in diesel engines have been checked by focusing on their regulated emissions and PAH emissions. Four bio-based long-chain ethers with similar structures, including: Polyoxymethylene dimethyl ether , diglyme , dipropylene glycol dimethyl ether and tripropylene glycol methyl ether have been blended with diesel fuel and tested in a small-duty diesel engine. The results showed that long-chain ethers were beneficial to the reduction of regulated emissions by comparing to pure diesel. Polyoxymethylene dimethyl ether and tripropylene glycol methyl ether showed best performance among the four tested ethers. Polyoxymethylene dimethyl ether could reduce 56% CO, 23% NO and 93% soot emissions, while Tripropylene glycol methyl ether could reduce 52% CO, 28% NO and 88% soot emissions. Besides, the particle sizes of soot particles from the blended fuels were also reduced. What's more, the addition of bio-based long-chain ethers could reduce particulate PAHs emissions by 39% ~ 67% and reduce gaseous PAHs emissions by 25% ~ 44%, and the PAHs toxicity was also reduced by 32% ~ 55%. This work proved that the structure of oxygen atoms evenly distributed in the chain could efficiently suppress the production of soot precursors and eventually reduce the soot emission
研究领域:
能源与环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Xudong Dong,Qibing Wang,Juan Peng,Min Wu,Bo Pan,Baoshan Xing
The accumulation and transfer of carcinogens, including polycyclic aromatic hydrocarbons (PAHs), in the human body, especially from mother to fetus, has been the subject of many research studies, but the related data are limited and the mechanisms are unknown. This is the first study to investigate the distribution of PAHs in paired samples of maternal blood, placenta tissue, and umbilical cord blood in relation to pregnancy complications. Sixty-four pairs of samples were collected in Kunming, China; 18 were from healthy pregnant women and 46 were from patients with pregnancy complications. The predominant PAHs in these pregnant women were high-molecular-weight (HMW) compounds, mainly from the incomplete combustion or pyrolysis of biomass. In the control group, the total amount of HMW compounds (Σ HMW PAHs) was significantly higher in maternal blood than in umbilical cord blood, which suggested that placenta may decrease PAH transfer in healthy pregnant women. However, this phenomenon was not observed for low-molecular-weight PAHs or in the case group. In the control group, Σ 16 PAH and Σ HMW PAH in the placenta were higher than those in maternal blood and umbilical cord blood; for the case group, a contrasting result was observed. Σ HMW PAHs in the placenta was significantly higher in the control group than in the case group. The same results were observed after the exclusion of the impact of the genotypes of the PAH metabolic enzymes (both phase I and phase II enzymes). Thus, the decreased PAH transfer from mother to fetus may partially result from the accumulation of PAHs inside the placenta.
研究领域:
环境科学
·
毒理学
·
妇产科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）