PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Jiahui Tong,Huijuan Guo,Jinzhi Zeng,Jiazeng Li,Zihua Zhao,Yucheng Sun
Aphids employ numbers of salivary proteins to subvert plant defences during phloem feeding. These salivary proteins can be secreted into plants, and localised to the mesophyll cells and the phloem, but few studies identify the effectors suppressing the apoplastic defence during feeding. This study found that a cuticular protein MpCP2 released from the green peach aphid Myzus persicae could stabilise cell wall cellulose by preventing its degradation. Salivary MpCP2 was secreted into the apoplast and bound with cellulose, which inhibited the enzymatic cleavage of cellulose into cellobiose. The reduced levels of cellobiose, a damage-associated molecular pattern (DAMP), alleviated downstream reactions of plants, including Ca 2+ sparks, reactive oxygen species production, and transcriptional levels of defensive genes WRKY30 , MYC2 and PAL . Knockdown of MpCP2 in aphids reduced the phloem ingestion time, and compromised the survival rate and fecundity of aphids. In contrast, overexpression of MpCP2 in plants prolonged aphid phloem feeding time and enhanced survival rate. These results reveal a novel mechanism that some effectors of piercing-sucking insects are utilised to reduce the releases of cell wall-derived DAMPs by binding with cellulose, thereby suppressing DAMP-triggered plant defences.
研究领域:
植物-昆虫互作
·
分子植物病理学
·
昆虫学
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植物防御机制
·
细胞壁生物学
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DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2023
·Vol. 46
·期 5
·1653-1670
·
IF 7.3
Cheng Zhang,Chenchen Tong,Lei Cao,Pengpeng Zheng,Xiaofeng Tang,Lihuan Wang,Min Miao,Yongsheng Liu,Shuqing Cao
Cadmium (Cd) is one of the most dangerous environmental pollutants among heavy metals, and threatens food safety and human health by accumulating in plant sink tissues. Here, we report a novel regulatory cascade that profoundly influences Cd tolerance in Arabidopsis. Phenotypic analysis showed that an insertional knockdown mutation at the Arabidopsis Tóxicos en Levadura 31 ( ATL31 ) locus resulted in hypersensitivity to Cd stress, most likely due to a significant increase in Cd accumulation. Consistently, ATL31 -overexpressing lines exhibited enhanced Cd stress tolerance and reduced Cd accumulation. Further, IRON-REGULATED TRANSPORTER 1 (IRT1) was identified, and yeast two-hybrid, co-immunoprecipitation and bimolecular fluorescence complementation assays demonstrated its interaction with ATL31. Biochemical, molecular, and genetic analyses showed that IRT1 is targeted by ATL31 for ubiquitin-conjugated degradation in response to Cd stress. Intriguingly, transcription of ATL31 was strongly induced by Cd stress. In addition, transgenic and molecular analyses showed that WRKY33 directly activated the transcription of ATL31 in response to Cd stress and positively regulated Cd tolerance. Genetic analysis indicated that ATL31 acts upstream of IRT1 and downstream of WRKY33 to regulate Cd tolerance. Our study revealed that the WRKY33-ATL31-IRT1 module plays a crucial role in timely blocking Cd absorption to prevent metal toxicity in Arabidopsis.
研究领域:
植物生物学
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分子遗传学
·
环境毒理学
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PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2023
·
IF 7.3
Yating Zhang,Xuguang Sun,Pedro J. Aphalo,Yuqi Zhang,Ruifeng Cheng,Tao Li
Plants adjust their morphology in response to light environment by sensing an array of light cues. Though the wavelengths of ultraviolet-A1 radiation (UV-A1, 350–400 nm) are close to blue light (B, 400–500 nm) and share same flavoprotein photoreceptors, it remains poorly understood how plant responses to UV-A1 radiation could differ from those to B. We initially grown tomato plants under monochromatic red light (R, 660 nm) as control, subsequently transferred them to four dichromatic light treatments containing ~20 µmol m −2 s −1 of UV-A1 radiation, peaking at 370 nm (UV-A 370 ) or 400 nm (V 400 ), or B (450 nm, at ~20 or 1.5 µmol m −2 s −1 ), with same total photon irradiance (~200 μmol m −2 s −1 ). We show that UV-A 370 radiation was the most effective in inducing light-intercepting leaf-area display formation, resulting in larger leaf area and more shoot biomass, while it triggered weaker and later transcriptome-wide responses than B. Mechanistically, UV-A 370 -promoted leaf-area display response was apparent in less than 12 h and appeared as very weakly related to transcriptome level regulation, which likely depended on the auxin transportation and cell wall acidification. This study revealed wavelength-specific responses within UV-A/blue region challenging usual assumptions that the role of UV-A1 radiation function similarly as blue light in mediating plant processes.
研究领域:
植物生理学
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分子生物学
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植物形态学
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光合作用
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PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2026
·
IF 6.3
Tao Chen,Shengchun Li,Xiujie Mu,Tianzhao Yang,Lijuan Wang,Mimi Tian,Mei Yu,Long Luo,Yuanming Xie,Wei Xuan,Graham Noctor,Yi Han
Accumulating evidence shows that reversible protein S -nitrosylation is essential for H 2 O 2 homoeostasis and signalling. However, roles for denitrosylation in such oxidative signalling remain poorly understood. Here, we examined this question using the Arabidopsis catalase-defective mutant, cat2 , in which oxidative stress induces both glutathione accumulation and salicylic acid (SA) pathways. Induction of these pathways was accompanied by enhanced thioredoxin ( TRXH5 ) expression, and oxidative stress-induced activation of the SA pathway was compromised when TRXH5 expression was genetically disabled, whereas TRXH5 overexpression stimulates H 2 O 2 -triggered SA responses. Intriguingly, TRX h5 -reinforced SA responses were antagonised by glutathione (GSH) deficiency when introducing additional pad2 mutation, localised in the GLUTAMATE-CYSTEINE LIGASE gene encoding the first enzyme of glutathione biosynthesis. Further analysis revealed that the two active cysteine residues of recombinant TRX h 5 can be denitrosylated by GSH. Blocking glutathione accumulation increased more TRX h 5-SNO formation in TRXH5-YFP cat2 pad2 trxh5 than in TRXH5-YFP cat2 trxh5 . Furthermore, S -nitrosoglutathione reductase (GSNOR) was capable of physically interacting with TRX h 5, and was also required for GSH-dependent TRX h 5 denitrosylation and TRX h 5-enhanced SA responses during oxidative stress. Collectively, these data suggest that GSH/GSNOR constitutes an active denitrosylating module that works together with the canonical NADPH-dependent TRX-reducing pathway to sustain cytosolic TRX h 5 operation within the oxidative signalling framework.
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高影响因子
PLANT CELL AND ENVIRONMENT
·
2021
·Vol. 44
·期 11
·3681-3699
·
IF 7.228
Yushan Zhai,Quan Yuan,Shiyou Qiu,Saisai Li,Miaomiao Li,Hongying Zheng,Guanwei Wu,Yuwen Lu,Jiejun Peng,Shaofei Rao,Jianping Chen,Fei Yan
Chloroplasts play crucial roles in plant defence against viral infection. We now report that chloroplast NADH dehydrogenase-like (NDH) complex M subunit gene ( NdhM ) was first up-regulated and then down-regulated in turnip mosaic virus (TuMV)-infected N. benthamiana . NbNdhM -silenced plants were more susceptible to TuMV, whereas overexpression of NbNdhM inhibited TuMV accumulation. Overexpression of NbNdhM significantly induced the clustering of chloroplasts around the nuclei and disturbing this clustering facilitated TuMV infection, suggesting that the clustering mediated by NbNdhM is a defence against TuMV. It was then shown that NbNdhM interacted with TuMV VPg, and that the NdhMs of different plant species interacted with the proteins of different viruses, implying that NdhM may be a common target of viruses. In the presence of TuMV VPg, NbNdhM, which is normally localized in the nucleus, chloroplasts, cell periphery and chloroplast stromules, colocalized with VPg at the nucleus and nucleolus, with significantly increased nuclear accumulation, while NbNdhM-mediated chloroplast clustering was significantly impaired. This study therefore indicates that NbNdhM has a defensive role in TuMV infection probably by inducing the perinuclear clustering of chloroplasts, and that the localization of NbNdhM is altered by its interaction with TuMV VPg in a way that promotes virus infection.
研究领域:
植物病毒学
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分子植物病理学
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叶绿体生物学
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植物免疫学
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分子病毒学
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DOI
高影响因子
PLANT CELL AND ENVIRONMENT
·
2024
·
IF 6
Huige Guo,Xiaochen Wang,Changlin Li,Hala F. Mohamed,Dawei Li,Lianghui Wang,Hongzhe Chen,Kunning Lin,Shuyuan Huang,Jinling Pang,Yuanbiao Zhang,Bernd Krock,Zhaohe Luo
Prevalent interactions among marine phytoplankton triggered by long-range climatic stressors are well-known environmental disturbers of community structure. Dynamic response of phytoplankton physiology is likely to come from interspecies interactions rather than direct climatic effect on single species. However, studies on enigmatic interactions among interspecies, which are induced by bioactive extracellular compounds (BECs), especially between related harmful algae sharing similar shellfish toxins, are scarce. Here, we investigated how BECs provoke the interactions between two notorious algae, Alexandrium minutum and Gymnodinium catenatum , which have similar paralytic shellfish toxin (PST) profiles. Using techniques including electron microscopy and transcriptome analysis, marked disruptions in G. catenatum intracellular microenvironment were observed under BECs pressure, encompassing thylakoid membrane deformations, pyrenoid matrix shrinkage and starch sheaths disappearance. In addition, the upregulation of gene clusters responsible for photosystem-I Lhca1/4 and Rubisco were determined, leading to weaken photon captures and CO 2 assimilation. The redistribution of lipids and proteins occurred at the subcellular level based on in situ focal plane array FTIR imaging approved the damages. Our findings illuminated an intense but underestimated interspecies interaction triggered by BECs, which is responsible for dysregulating photosynthesis and organelle function in inferior algae and may potentially account for fitness alteration in phytoplankton community.
研究领域:
海洋生物学
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环境科学
·
分子生态学
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DOI
高影响因子
PLANT CELL AND ENVIRONMENT
·
2026
·
IF 6.9
Huan Chen,Qinyu Deng,Lijie Yang,Mengqi Hao,Linxi Pan,Qing Gao,Ran Li,Yonggen Lou,Jing Lu
Nitrogen level significantly impacts rice resistance to the brown planthopper (BPH, Nilaparvata lugens ). Yet, the mechanisms underlying nitrogen level-mediated rice BPH resistance remain largely unclear. Here, we found that, relative to medium nitrogen (MN) and high nitrogen (HN) conditions, low nitrogen (LN) suppressed rice growth but enhanced plant resistance to BPH, as evidenced by reduced nymph biomass, decreased phloem ingestion and lower fecundity. Biochemical and transcriptome analyses revealed that compared to MN and HN plants, LN plants exhibited significantly higher basal and/or BPH-induced levels of abscisic acid (ABA) and salicylic acid (SA), cell wall components (lignin, cellulose and hemicellulose), and certain flavonoids but lower levels of phenolamides. Moreover, the relative water content (RWC) and total amino acid concentrations were significantly lower in LN plants compared with those in MN and HN plants. Impairing the signalling pathway mediated by ABA but not SA in rice abolished the basal and/or BPH-induced accumulation of defence compounds (cell wall components and certain flavonoids) in plants and plant resistance to BPH. The results demonstrate that the ABA signalling pathway, together with reduced RWC and total amino acid concentrations in plants caused by LN conditions, plays an important role in regulating the LN-mediated rice resistance to BPH.
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PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Xin Wen,Chao He,Yuhao Fu,Leilei Xiang,Jean Damascene Harindintwali,Yu Wang,Siqi Wei,Maoyuan Liao,Jiandong Jiang,Xin Jiang,Ravi Naidu,Fang Wang
Shifting from maize monoculture to maize-soybean rotation or intercropping is a key strategy to boost soybean self-sufficiency. However, the residual atrazine in soils, resulting from the extensive use of atrazine in long-term maize monocultures, poses a significant threat to soybean productivity, requiring immediate solutions. This study introduces a novel seed-coating method using atrazine-degrading Paenarthrobacter sp. AT5 to mitigate the adverse effects of atrazine on soybean growth. The efficacy of seed coating in reducing atrazine accumulation in seedlings and promoting seedling growth was found to vary depending on the growth stage and atrazine concentration in the soil. At low atrazine concentrations (close to safe levels for soybeans), the seed coating reduced leaf atrazine concentrations by 30.7%–46.0% at the cotyledon growth stage (VC). At high atrazine concentrations, the shoot atrazine concentrations decreased by 60.9%–88.4% at the emergence growth stage (VE) and seedling biomass was significantly increased in the VC stage. This is associated with the influence of seed coating on rhizosphere metabolites, which activate the plant defence system and recruit atrazine-degrading and plant-beneficial bacteria. This study offers comprehensive insights into the potential of seed coating with strain AT5 as a scalable solution for mitigating atrazine toxicity in soybeans.
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PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Chenxi Gao,Zhihui Wang,Zhuotong Chen,Qian Zhang,Biyun He,Zhiqiang Zheng,Zhe Zhou,Yan Huang,Shixian Cao,Zhidan Chen,Weijiang Sun
Tea plants with distinct leaf colours, such as purple, have attracted attention for their potential economic and flavour-related benefits. To investigate how purple mutations influence aroma composition, we analyzed 64 tea germplasms ( Camellia sinensis ) with varying degrees of purple pigmentation, integrating microenvironmental data, volatile metabolomics and transcriptomics. Purple tea exhibited reduced accumulation of volatile phenylpropanoids/benzenoids (VPBs), higher surface temperatures and enrichment of stress-responsive genes, leading to increased levels of fatty acid-derived volatiles (FADVs) and volatile terpenoids (VTs). We identified CsWRKY70 as a central regulator of aroma biosynthesis, with its expression upregulated under heat stress, activating CsLOX3 and CsTPS13 to promote FADVs and VTs accumulation. These findings demonstrate that purple leaf mutations influence aroma composition by modulating the phenylpropanoid pathway and altering the leaf microenvironment, providing new insights into the interplay between leaf colour, environmental stress and flavour potential in tea plants.
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PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Zhiquan Qiang,Zhen Zeng,Danfeng Ma,Jinzhe Li,Yuang Zhao,Tao Qin
Drought serves as a major environmental stress that restricts both the yield and quality of perennial ryegrass. Therefore, it is important to identify the essential genes that determine drought tolerance in perennial ryegrasses. In this paper, we isolated a drought-induced NAC transcription factor LpNAC22 . Transcriptional activity assays in yeast and plant cells indicated that LpNAC22 has transcriptional activation function. Subcellular localization observations revealed that LpNAC22 localized in the nucleus, compatible with its function as a transcription factor. LpNAC22 overexpression plants had enhanced drought tolerance and reduced cell membrane damage, whereas the knockdown of LpNAC22 in perennial ryegrass reduced plant drought tolerance and led to aggravated cell membrane damage. Late embryogenesis abundant (LEA), well-known stress resistance proteins, can protect the cell membrane from damage during drought conditions. DNA affinity purification sequencing and transcriptional regulation analysis demonstrated that LpNAC22 upregulates two LEA family genes, LpLEA1 and LpLEA2-1 , by directly binding to their promoters. Furthermore, we demonstrated that overexpression of LpLEA1 and LpLEA2-1 in Arabidopsis enhanced drought tolerance and reduced cell membrane damage under drought conditions. Our findings provide evidence that LpNAC22 improves drought resistance by modulating the transcription of LEA family genes in perennial ryegrass.
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PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2024
·Vol. 47
·期 3
·913-927
·
IF 6
Guanhua Liu,Qian Wang,Hui Chen,Yuxi Wang,Xiaogui Zhou,Demeng Bao,Nuo Wang,Juan Sun,Fuyin Huang,Mei Yang,Han Zhang,Peng Yan,Xin Li,Jiang Shi,Jianyu Fu
Insect-induced plant volatile organic compounds (VOCs) may function as either direct defence molecules to deter insects or indirect defence signals to attract the natural enemies of the invading insects. Tea ( Camellia sinensis L.), an important leaf-based beverage crop, is mainly infested by Ectropis obliqua which causes the most serious damage. Here, we report a mechanistic investigation of tea plant-derived VOCs in an indirect defence mechanism against E. obliqua . Parasitoid wasp Parapanteles hyposidrae , a natural enemy of E. obliqua , showed strong electrophysiological response and selection behaviour towards S -linalool and β-ocimene, two monoterpenes with elevated emission from E. obliqua -damaged tea plants. Larvae frass of E. obliqua , which also released S -linalool and β-ocimene, was found to attract both mated female or male Pa. hyposidrae according to gas chromatography-electroantennogram detection and Y-tube olfactometer assays. In a field setting, both S -linalool and β-ocimene were effective in recruiting both female and male Pa. hyposidrae wasps. To understand the molecular mechanism of monoterpenes-mediated indirect defence in tea plants, two novel monoterpene synthase genes, CsLIS and CsOCS-SCZ , involved in the biosynthesis of S -linalool or β-ocimene, respectively, were identified and biochemically characterised. When the expression of these two genes in tea plants was inhibited by antisense oligodeoxynucleotide, both volatile emission and attraction of wasps were reduced. Furthermore, gene expression analysis suggested that the expression of CsLIS and CsOCS-SCZ is regulated by the jasmonic acid signalling pathway in the tea plant.
研究领域:
植物学
·
昆虫学
·
分子生物学
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DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2021
·Vol. 44
·期 9
·3173-3183
·
IF 7.228
Kunlong Su,Zhenying Wu,Yuchen Liu,Shanshan Jiang,Dongmei Ma,Yan Wang,Chunxiang Fu
Dinitrotoluene (DNT) has been extensively used in manufacturing munitions, polyurethane foams and other important chemical products. However, it is highly toxic and mutagenic to most organisms. Here, we synthesized a codon-optimized bacterial nitroreductase gene, NfsI, for plant expression. The kinetic analysis indicates that the recombinant NfsI can detoxify both 2,4-DNT and its sulfonate (DNTS), while it has a 97.6-fold higher catalytic efficiency for 2,4-DNT than DNTS. Furthermore, we overexpressed NfsI in switchgrass ( Panicum virgatum L.), which is a multiple-purpose crop used for fodder and biofuel production as well as phytoremediation. The 2,4-DNT treatment inhibited root elongation of wild-type switchgrass plants and promoted reactive oxygen species (ROS) accumulation in roots. In contrast, overexpression of NfsI in switchgrass significantly alleviated 2,4-DNT-induced root growth inhibition and ROS overproduction. Thus, the NfsI overexpressing transgenic switchgrass plant removed 94.1% 2,4-DNT after 6 days, whose efficiency was 1.7-fold higher than control plants. Moreover, the comparative transcriptome analysis suggests that 22.9% of differentially expressed genes induced by 2,4-DNT may participate in NfsI-mediated 2,4-DNT detoxification in switchgrass. Our work sheds light on the function of NfsI during DNT phytoremediation for the first time, revealing the application potential of switchgrass plants engineered with NfsI .
研究领域:
植物学
·
环境科学
·
遗传工程
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DOI
高影响因子
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