NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 14
·6700-6708
·
IF 3.269
Huimin Zhao,Yaxi Li,Bing Tan,Yaobin Zhang,Xiaochi Chen,Xie Quan
To improve the peroxidase-like activity of molybdenum dichalcogenides (MoS2), surface modification, such as combing with monometallic or bimetallic nanoparticles, is of great and widespread interest. However, only a few studies have been reported on the modification of MoS2 with non-metallic materials. Herein, we report a study on the peroxidase-like activity of novel PEGylated MoS2 (PEG-MoS2) nanosheets prepared using a simple liquid-phase exfoliation and pegylation process. Compared to the undecorated MoS2 nanosheets, PEG-MoS2 nanosheets have improved water dispersiblity and stability, which are beneficial for improving the peroxidase-like activity, as well as the catalysis velocity and affinity for TMB or H2O2. Based on PEGylated MoS2, we successfully established a new platform for the colorimetric detection of H2O2 with a limit of detection (LOD) of 1.18 μM, which is comparable to that of the majority of the reported enzyme mimics of inorganic graphene analogues. More importantly, the as-prepared PEG-MoS2 nanosheets are environmentally friendly, water dispersible, low cytotoxic, and easily prepared, which indicate that PEG-MoS2 is a type of promising enzyme mimics and extremely likely to be applied in cell-based assay systems without any adverse reactions.
研究领域:
生物化学
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纳米医学
·
分析化学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2014
·Vol. 39
·期 1
·589-594
·
IF 3.159
Zhaogui Ba,Yumin Zhang,Junpei Wei,Jiwu Han,Zhenqiang Wang,Guangrui Shao
Nanoparticulate contrast agents have drawn considerable attention and interest because of their potential application in medical diagnosis and prognosis. In the present study, we designed and constructed high-performance nanoparticulate contrast agents based on PEGylated lutetium hydroxycarbonate nanoparticles (PEG-LuNPs) for X-ray computed tomography imaging, which were synthesized via a green and large-scale route. Under the daily clinical voltage, our PEG-LuNPs provided much more enhanced contrast than the routinely used iodine-based molecules. More importantly, PEG-LuNPs could act as liver-targeted contrast agents for the further detection of hepatic metastases. Both in vitro and in vivo toxicity studies indicated that these nanoparticles possess extremely high biocompatibility, revealing their overall safety. Based on these results, PEG-LuNPs with intrinsic physicochemical properties and excellent imaging capability demonstrated a useful nanoplatform for biomedical applications.
研究领域:
医学影像学
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生物材料学
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纳米医学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2015
·Vol. 39
·期 11
·8999-9005
·
IF 3.086
Yingda Du,Ming Xing,Zhiman Li,Wei Guo
Along with the rapid development of modern medicine, the creation and evaluation of nanoparticulate contrast agents have attracted considerable attention of scientists worldwide. Herein, we designed and synthesized a metabolizable nano-sized contrast agent based on rod-like PEGylated gadolinium hydroxide (defined as PEG-Gd(OH)3) via a facile and economic route. Due to the high amount of gadolinium in these nanorods, these well-prepared PEG-Gd(OH)3 could provide more enhanced contrast than clinical iodine-based agents under the usual voltages in computed tomography (CT) imaging. Different from other small molecule-based contrast agents, our PEG-Gd(OH)3 exhibited an obvious liver-targeted property based on our in vivo experiments, indicating their expected usage in the detection of hepatic metastases. Long-term toxicity and possible metabolic pathway demonstrated that these nanorods exhibit overall safety and could not cause gadolinium-associated nephrogenic systemic fibrosis (NSF). With its high biocompatibility and superior contrast efficacy, PEG-Gd(OH)3 could bring more opportunities to a new generation of nanoparticulate CT contrast agents in biomedicine.
研究领域:
医学影像学
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纳米医学
·
生物材料学
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产品关联
DOI
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2012
·Vol. 12
·期 4
·533-546
·
IF 3.886
Jinshan Guo,Fanbo Meng,Xiaoyuan Li,Mingzhe Wang,Yanjuan Wu,Xiabin Jing,Yubin Huang
PEGylated click polypeptides ( PEG-CP s) containing α -amino side groups as well as PEG segments are designed for selective endotoxin removal from protein solutions. The PEG-CP s are synthesized via copper-free thermal click copolymerization from aspartic (or glutamic) acid-based dialkyne and diazide monomers (containing free amino side groups) and alkyne-terminated mPEGs or dialkyne-terminated PEGs. Microwave-assisting technology is introduced into thermal click chemistry to improve the reaction efficiency. The monomers and polymers are fully characterized using NMR, XPS, and MALDI-TOF MS. After immobilizing the PEGylated click polypeptides onto polystyrene microspheres, the adsorbents exhibit good endotoxin removal selectivity from BSA solutions.
研究领域:
生物化学
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材料科学
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2016
·
IF 3.15
Peng Gao,Meiying Liu,Jianwen Tian,Fengjie Deng,Ke Wang,Dazhuang Xu,Liangji Liu,Xiaoyong Zhang,Yen Wei
Graphene oxide (GO) based polymer nanocomposites have attracted extensive research interest recently for their outstanding physicochemical properties and potential applications. However, surface modification of GO with synthetic polymers has demonstrated to be trouble for most polymerization procedures are occurred under non-aqueous solution, which will in turn lead to the restacking of GO. In this work, a facile and efficient “one-pot” strategy has been developed for surface modification of GO with synthetic polymers through single-electron-transfer living radical polymerization (SET-LRP). The GO based polymer nanocomposites were obtained via SET-LRP in aqueous solution using poly(ethylene glycol) methyl ether methacrylate (PEGMA) as the monomer and 11-bromoundecanoic acid as the initiator, which could be effectively adsorbed on GO through hydrophobic interaction. The successful preparation of GO based polymer nanocomposites was confirmed by a series of characterization techniques such as 1 H nuclear magnetic resonance, Fourier transform infrared spectroscopy, thermogravimetric analysis, transmission electron microscopy and X-ray photoelectron spectroscopy. The resultant products exhibit high water disperisibility, excellent biocompatibility and high efficient drug loading capability, making these PEGylated GO nanocomposites promising candidates for biomedical applications.
研究领域:
材料科学
·
生物医学工程
·
化学
✓ 已验证
DOI
高影响因子
ACS Nano
·
2024
·
IF 15.8
Hui-Yue Zhao,Yuan-Qiang Chen,Xing-Yu Luo,Ming-Jie Cai,Jia-Yi Li,Xin-Yu Lin,Hao Zhang,Hong-Ming Ding,Guang-Liang Jiang,Yong Hu
Neutral nanomaterials functionalized with PEG or similar molecules have been popularly employed as nanomedicines. Compared to positive counterparts that are capable of harnessing the well-known proton sponge effect to facilitate their escape from lysosomes, it is yet unclear how neutral substances got their entry into the cytosol. In this study, by taking PEGylated, neutral Au nanospheres as an example, we systematically investigated their time-dependent translocation postuptake. Specifically, we harnessed dissipative particle dynamics simulations to uncover how nanospheres bypass lysosomal entrapment, wherein a mechanism termed as “squeezing-out” mode was discovered. We next conducted a comprehensive investigation on how nanomaterials implicate lysosomes in terms of integrity and functionality. By using single-molecule imaging, specific preservation of PEG-terminated with targeting moieties in lysosomes supports the “squeezing-out” mode as the mechanism underlying the lysosomal escape of nanomaterials. All evidence points out that such a process is benign to lysosomes, wherein the escape of nanomaterials proceeds at the expense of targeting moieties loss. Furthermore, we proved that by fine-tuning of the efficacy of nanomaterials escaping from lysosomes, modulation of distinct pathways and metabolic machinery can be achieved readily, thereby offering us a simple and robust tool to implicate cells.
研究领域:
纳米技术
·
生物物理学
·
药学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Soft Matter
·
2020
·Vol. 16
·期 24
·5750-5758
·
IF 3.14
Jinxuan Guo,Zujian Feng,Xiang Liu,Changrong Wang,Pingsheng Huang,Jianhua Zhang,Liandong Deng,Weiwei Wang,Anjie Dong
We synthesized amino-modified poly(ε-caprolactone) PCN-b-PEG-b-PCN (PECN) triblock copolymers and studied the contribution of the introduced amino groups to the drug delivery efficiency of PECN nanoparticles (NPs) and their injectable thermosensitive hydrogels. PECN15 with an optimal amino group content was obtained. Firstly, the hydrophobic drug paclitaxel (PTX) was loaded into PECN15 up to 5.91% and formed PTX/PECN NPs 90 nm in size and with a slightly positive charge (7.3 mV). Furthermore, the injectable PTX/PECN NPs aqueous solution (25 wt%) at ambient temperature could undergo fast gelation at 37 °C and sustainedly release PTX/PECN NPs in 10 days. More importantly, compared with our previously reported PECT NPs, the PECN NPs without an increase in toxicity could improve the cell uptake and enhance intracellular drug release by responding to the acidic environment of the endosome. Thus, the PTX/PECN NPs presented a lower IC50 of 3.14 μg mL−1 than that of the PTX/PECT NPs (7.67 μg mL−1) and free PTX (4.65 μg mL−1). Moreover, through peritumoral injection, the PTX/PECNGel showed 94.27% inhibition rate of tumor growth on day 19, higher than PTX/PECTGel (72.28%) and Taxol® (47.03%). Therefore, the PECN NPs hydrogel provided a more effective injectable platform to enhance local cancer chemotherapy, and also provided the possibility of further functionalization by the reactive amino groups.
研究领域:
药学
·
纳米技术
·
癌症研究
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2017
·
IF 7.786
Zhi-qiang Yuan,Wei-liang Chen,Ben-gang You,Yang Liu,Shu-di Yang,Ji-zhao Li,Wen-jing Zhu,Xiao-feng Zhou,Chun Liu,Xue-nong Zhang
Malignant proliferation and metastasis in non-small cell lung carcinoma (NSCLC) are great challenges for effective clinical treatment through conventional chemotherapy. The combinational therapy strategy of RNA interfering (RNAi) technology and chemotherapeutic agents have been reported to be promising for effective cancer therapy. In this study, based on multifunctional nanoparticles (NPs), the simultaneous delivery of etoposide (ETP) and anti-Enhancer of Zeste Homologue 2 (EZH2) siRNA for the effective treatment of orthotopic lung tumor was achieved. The NPs exhibited pH/redox dual sensitivity verified by particle size changes, morphological changes, and in vitro release of drugs. Confocal microscopy analysis confirmed that the NPs exhibited endosomal escape property and on-demand intracellular drug release behavior, which can protect siRNA from degradation and facilitate the chemotherapeutic effect respectively. In vitro tumor cell motility study demonstrated that EZH2 siRNA loaded in NPs can decrease the migration and invasion capabilities of tumor cells by downregulating the expression of EZH2 mRNA and protein. In particular, an antiproliferation study revealed that the co-delivery of siRNA and ETP in the multifunctional NPs can induce a synergistic therapeutic effect on NSCLC. In vivo targeting evaluation showed that cRGDyC-PEG modification on NPs exhibited a low distribution in normal organs and an obvious accumulation in orthotopic lung tumor. Furthermore, targeted NPs co-delivering siRNA and ETP showed superior inhibition on tumor growth and metastasis and produced minimal systemic toxicity. These findings indicated that multifunctional NPs can be utilized as a co-delivery system, and that the combination of EZH2 siRNA and ETP can effectively treat NSCLC.
研究领域:
药学
·
纳米技术
·
肿瘤学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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