PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2024
·
IF 2.6
Yu Li,Miao Guo,Li Li,Fan Yang,Lidan Xiong
Photoaging, caused by ultraviolet (UV) radiation, is characterized by the senescence of skin cells and reduction of collagens. Although rice fermentation is widely used in the cosmetics, its impact on skin photoaging is still not well understood. Herein, we investigated the possible effectiveness of Maifuyin, a fermented rice product, and its components, succinic acid (SA), and choline, for safeguarding UVA-exposed human dermal fibroblasts (HDFs) against photoaging. In this study, the effects of Maifuyin, SA, and choline on UVA-induced cell death and senescence in fibroblasts were evaluated in cell counting kit-8 (CCK-8), expression of β-galactosidase (β-GAL), and matrix metalloproteinases (MMP)-1. To identify oxidative stress, the investigation focused on reactive oxygen species, glutathione, superoxide dismutase, and malondialdehyde. Additionally, a mRNA sequencing technology (RNA-seq) was applied to study the underlying mechanisms of these components on UVA-induced photoaging. Meanwhile, the level of C–X–C motif chemokine ligand 2 (CXCL2) in the cell supernatant was confirmed to assess the autocrine chemokine level. To reassess the involvement of CXCL2, the expression of β-GAL was evaluated in fibroblasts treated with or without CXCL2. The results indicated that 1 mg/mL Maifuyin and SA inhibited UVA-induced senescence in fibroblasts, MMP-1 expression, and oxidative damage. The RNA-seq revealed 1 mg/mL Maifuyin and SA might be recruited chemokine CXCLs to inhibit MMPs production and fibroblast senescence via TNFα, MAPK, and NF-κB pathways. ELISA results showed a significant reduction of autocrine CXCL2 in UVA-irradiated HDFs by pretreating Maifuyin and SA. The β-GAL staining assay revealed that CXCL2 treatment increased β-GAL activity, while the administration of Maifuyin and SA counteracted this effect in HDFs. These results highlighted the potential use of Maifuyin and SA as promising candidates for anti-photoaging applications.
研究领域:
美容学
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细胞生物学
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药理学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2024
·
IF 2.6
Xiuping Lin,Chang-sheng Shao,Shereen M. Elsherbiny,Qing Huang
Astaxanthin (AST) is a xanthophyll carotenoid with strong oxidation resistance, which can effectively scavenge various free radicals and protect organisms from oxidative damage. AST is also known to have prominent anti-aging effects, but the underlying mechanism of AST in anti-radiation aging is largely unknown. In this work, we applied ultraviolet (UV) irradiation to accelerate the aging of Caenorhabditis elegans ( C. elegans ) and treated the nematodes with AST to explore whether and how AST could attenuate the radiation-induced aging effect. Our results showed that AST improved the survival rate of C. elegans , reduced the aging biomarkers, and alleviated the mitochondrial dysfunction caused by the irradiation. Based on the transcriptome sequencing analysis, we identified that the key genes regulated by AST were involved in JNK-MAPK and DAF-16 longevity signaling pathways. Furthermore, we employed jnk-1 and daf-16 mutants and verified the role of the JNK-1/DAF-16 signaling pathway in the anti-aging effect. As such, this study has not only demonstrated that AST can resist the aging process caused by UV-irradiation but also revealed the anti-aging mechanism of AST through JNK-1/DAF-16 activation in C. elegans.
研究领域:
分子生物学
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衰老研究
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遗传学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2024
·
IF 2.6
Ting-Ting Li,Bing-Bing Shou,Lu Yang,Hai-Tao Ren,Xian-Jin Hu,Jia-Horng Lin,Tao Cai,Ching-Wen Lou
Combining photodynamic antimicrobials with nonwovens is prospective. However, common photosensitizers still have drawbacks such as poor photoactivity and the inability to charge. In this study, a photodynamic and high-efficiency antimicrobial protective material was prepared by grafting bis benzophenone-structured 4,4-terephthaloyl diphthalic anhydride (TDPA) photosensitizer, and antimicrobial agent chlorogenic acid (CA) onto spunbond-meltblown-spunbond (SMS) membranes. The charging rates for · OH and H 2 O 2 were 6377.89 and 913.52 μg/g/h. The light absorption transients structural storage remained above 69% for 1 month. High electrical capacity remained after seven cycles indicating its rechargeability and recyclability. The SMS/TDPA/CA membrane has excellent bactericidal performance when under illumination or lightless conditions, and the bactericidal efficiency of Escherichia coli and Staphylococcus aureus reached over 99%. The construction of self-disinfection textiles based on the photodynamic strategies proposed in this paper is constructive for expanding and promoting the application of textile materials in the medical field.
研究领域:
材料科学
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纺织工程
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光动力学
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抗菌技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2016
·Vol. 92
·期 4
·515-522
·
IF 2.008
Yanmei Luo,Yiru Jia,Dexian Zhang,Xinjian Cheng
TiO 2 /PVA composite nanofiber mat was prepared via an electrospinning technology. SH-TiO 2 -SiO 2 hybrid particles and PVA solution were injected through a coaxial syringe, yielding a composite nanofiber mat. The as-prepared SH-TiO 2 -SiO 2 /PVA composite nanofiber mat was immersed in Cd 2+ cation solution and S 2− anion solution in turn. Thus, yellow TiO 2 @CdS/PVA composite nanofiber mats were prepared. By adjusting the number of times a mat was immersed in the Cd 2+ and S 2− solutions, different amounts of CdS particles attaching to the mats were obtained. Both SH-TiO 2 -SiO 2 /PVA and TiO 2 @CdS/PVA composite nanofiber mats were employed to catalyze the photodegradation of a model dye, methylene blue. The photodegradation performance could be greatly enhanced by the introduction of CdS particles anchoring onto TiO 2 particles. The photodegradation efficiency reached 99.2% within 180 min. Also, the nanofiber mat could be recycled and reused at least 10 times. The photodegradation efficiency of TiO 2 @CdS/PVA composite nanofiber mats remained 68.8% for 10 cycles.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2013
·Vol. 90
·期 3
·496-502
·
IF 2.287
Kun Li,Qin Zhang,Mei Wang,Rongrong Zhu,Xiaoyu Sun,Xianzheng Wu,Shi-Long Wang
Tanshinone IIA (Tan IIA) has the properties of cardiovascular protection, anti-inflammation, antioxidation and anticancer. Its light-induced instability has drawn our interests in its photochemistry. Therefore, laser flash photolysis herein was used to investigate the transient photochemistry of Tan IIA. Our results show that direct photoexcitation by 355 nm laser pulses or photosensitization by energy transfer can lead to the formation of the triplet state of Tan IIA ( 3 Tan IIA*). The triplet absorption spectrum and molar absorption coefficient, and ISC quantum yield were determined. Self-quenching of 3 Tan IIA* by its ground state was identified as an autooxidation reaction. 3 Tan IIA* was proved to react quickly with N , N -dimethylaniline, tert-butylhydroquinone and propyl gallate via electron transfer with the diffusion-controlled rate constants. One of the products with maximum absorption around 390 nm was assigned to the radical anion of Tan IIA. Our results indicate that 3 Tan IIA* is a reactive transient species and can be generated by photosensitization or direct photoexcitation. According to our results, the possible role of Tan IIA as a photosensitizer to induce potential phototoxicity via Type-II pathway in the presence of O 2 can be predicted.
研究领域:
药理学
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光化学
·
生物医学工程
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DOI
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2021
·Vol. 97
·期 4
·841-850
·
IF 3.421
Li Juan,Wei Diandian,Wang Jianfeng,Li Ning,Fan Yuchen,Li Na,Zhao Sijie,Li Kun,Sun Fengyuan
Tanshinone IIA (TanIIA) has multiple biological functions and already been clinically used to treat many cardiovascular diseases. TanIIA is a photoactive molecule and can be excited by light to generate 3 TanIIA*. Generation of 3 TanIIA* by TanIIA photosensitization indicates that TanIIA may serve as a photosensitizer to bring photodynamic damage to organisms. Therefore, human choroidal melanoma MUM-2B cell was chosen as a superficial tumor model and the photodynamic effect of TanIIA on tumor cells was evaluated in this study. The results showed that TanIIA photosensitization could generate singlet oxygen in noncellular system. MTT, clone formation and wound-healing assays showed that the survival and migration of MUM-2B cells could be efficiently inhibited by TanIIA photosensitization. And then, laser confocal microscope and flow cytometry were used to try to elucidate related mechanism. It was found that TanIIA could pass through cellular membrane and preferably accumulate in nucleus. TanIIA photosensitization could efficiently induce cell apoptosis and necrosis, increase intracellular ROS levels, decrease mitochondria membrane potential, and lead to cell cycle arrest in G2/M phase. Our findings indicate that TanIIA photosensitization can exert remarkable toxicity on choroidal melanoma cells.
研究领域:
肿瘤学
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药理学
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细胞生物学
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光化学
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2020
·Vol. 97
·期 2
·398-407
·
IF 2.721
Zhang Huilan,Wang Juan,Zhang Wen,Han Dong,Zhang Aiping
In the paper, a composite TiO 2 /SiO 2 -NHOC-FA was prepared using the coupling method which is a folic acid-targeted silica-coated titanium dioxide. Their structures were characterized by Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV-vis), X-ray diffraction (XRD), transmission electron microscope (TEM) and zeta potential method. The results showed that the average diameter of the prepared TiO 2 /SiO 2 -NHOC-FA composite is 18 nm, which is spherical. Compared with unmodified TiO 2 , TiO 2 /SiO 2 -NHOC-FA composite had superior water solubility and dispersion, and enhanced its photokilling activity by folic acid-targeted to FR (+) cells. In addition, photocatalytic TiO 2 /SiO 2 -NHOC-FA arrested cell cycle in G2/M phase of MCF-7 cells, resulting in a significant reduction of mitochondrial membrane potential (MMP), and also made the apoptosis rate, ROS components and intracellular calcium concentration increased. It killed the MCF-7 cells through apoptosis pathway. These results for the TiO 2 /SiO 2 -NHOC-FA composite can provide a theoretical basis for the photodynamic development of TiO 2 .
研究领域:
纳米技术
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癌症研究
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光催化
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2020
·Vol. 96
·期 5
·1154-1160
·
IF 2.721
Kaili He,Xin Zhan,Lian Liu,Xiaohong Ruan,Yiwei Wu
A simple and effective ratiometric fluorescence sensor of CdTe QDs/GCNNs for on-site and rapid analysis of Cu 2+ has been established by mixing physically CdTe QDs and graphite carbon nitride (GCNNs). Two emissions peaks of CdTe QDs at 572 nm and GCNNs at 436 nm are both excitated at 340 nm. Under a UV lamp, fluorescent of traffic yellow CdTe QDs is linearly quenched by Cu 2+ (as the detection signal), while blue GCNNs remains unchanged (as the reference), resulting in a distinguishable color change gradually from pink yellow to blue. The limit of detection (LOD) of this new sensor for Cu 2+ is as low as 0.47 ng mL −1 with 1.4 % RSD. The established method has been successfully applied to detection of Cu 2+ in various drinks with satisfactory results. Moreover, a paper-based sensor, which has been prepared by soaking cellulose acetate membrane in CdTe QDs/GCNNs sensor solution, has a wide semiquantitative detection range for Cu 2+ (0.01 ~ 5.0 μg mL −1 ). It has realized successfully on-site and rapid determination of Cu 2+ in red wine without any pretreatment procedure and is of great promotion and application value in determination of Cu 2+ in liquid samples.
研究领域:
分析化学
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环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2020
·Vol. 96
·期 5
·1088-1095
·
IF 2.721
Lirong Ma,Xuanlin Yang,Xiuzhong Yao,Weiyu Weng
Hexyl aminolevulinate (HAL) is a lipophilic derivative of 5-aminolevulinic acid (5-ALA) and can induce more protoporphyrin IX (PpIX) formation and stronger fluorescence intensity (FI) than 5-ALA, which will greatly facilitate photodynamic diagnosis and therapy. The main drawback of HAL is its low solubility in neutral aqueous media. In this study, surfactants were used to increase HAL solubility in the cell culture medium and serum, followed by in vitro fluorescence formation measurement in human pancreatic cancer cells (SW1990) and in vivo fluorescence detection in tumor-bearing mice. The results showed that Tween 80 (TW80) and Kolliphor® HS 15 (HS15) increased the solubility of HAL in the selected media. Although TW80 and HS15 exhibited in vitro cytotoxicity at high concentrations (5 mg mL −1 ), they facilitated fluorescent signal formation at the early stage of cell incubation. When surfactants were used, the FI should be determined without the routine washing process because surfactant-containing culture medium caused the loss of synthesized PpIX during the washing process. When HAL dissolved in TW80 solution was injected intraperitoneally into pancreatic cancer-bearing mice at a dose of 50 mg kg −1 , the tumors exhibited red fluorescence, which indicated that systemic administration of surfactant-solubilized HAL might be applicable for tumor fluorescence detection in vivo .
研究领域:
生物化学
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医学
·
药理学
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2018
·Vol. 95
·期 2
·501-511
·
IF 2.214
Shukun Le,Wenjing Li,Yue Li,Burenbayaer Borjigin,Guangshe Li,Xiaojing Wang
Ag 3 VO 4 /mpg-C 3 N 4 (mesoporous graphitic carbon nitride) heterojunction photocatalysts were prepared by anchoring tiny Ag 3 VO 4 particles on the nanosheet of mpg-C 3 N 4 . The prepared Ag 3 VO 4 /mpg-C 3 N 4 heterojunctions were used to remove tetracycline (TC), a kind of antibiotics widely released into the aquatic environment under solar irradiation. Compared with pure mpg-C 3 N 4 and Ag 3 VO 4 , Ag 3 VO 4 /mpg-C 3 N 4 displayed much higher photocatalytic activity (83.2% removal rate within 90 min under visible-light irradiation). Importantly, no apparent deactivation was observed for Ag 3 VO 4 /mpg-C 3 N 4 -40 after five cycles, inferring a good reusability. As confirmed by photocurrent measurement and photoluminescence spectra, the excellent photocatalytic property of Ag 3 VO 4 /mpg-C 3 N 4 was credit to the electron–hole separation enhancement at the formed heterojunction of two semiconductors. In addition, a possible mechanism and intermediate products for the Ag 3 VO 4 /mpg-C 3 N 4 photocatalysts toward the photodegradation of TC in aqueous solution under artificial sunlight radiation were proposed based on the scavengers trapping test, ESR spectra and a high-performance liquid chromatography (HPLC) coupled with mass spectrometer (MS) analysis. This investigation provides a low cost, green and easily practical approach to remove the antibiotics in the aquatic environment.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2022
·
IF 3.521
Yu Zhang,Dekun Cheng,Zhangtie Wang,Yiwen Guo,Ming Chang,Ruijie Liu
Excessive ultraviolet (UV) exposure is the primary environmental factor that contributes to skin aging. Orbitides have been considered as important functional components in linseed, and they are complex and multiple. In this study, linseed orbitides (LOs) were divided into oxidized linseed orbitides (OLOs) and reduced linseed orbitides (RLOs) and used to investigate protection against ultraviolet B-induced photoaging in zebrafish. First, the results of the zebrafish embryo acute toxicity test showed that the OLOs had obvious embryo toxicity compared with RLOs. And RLOs had better effect in ultraviolet B-treated zebrafish, which may significantly reduce the reactive oxygen species levels and inhibit the degradation of collagen. Besides, we also found that RLOs could effectively inhibit the oxidation of proteins and lipids and regulate the activity of antioxidant enzymes. Furthermore, neutrophil recruitment to the dorsal and caudal fin regions was observed in UVB-treated zebrafish, and RLOs effectively alleviated this migration. In conclusion, RLOs have strong photoprotective effects and potential to be used as antiphotoaging ingredients.
研究领域:
药理学
·
毒理学
·
皮肤科
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2015
·Vol. 91
·期 4
·814-818
·
IF 2.266
Kai Zheng,Kai-Li Lou,Cheng-Hui Zeng,Sha-Sha Li,Zhi-Wen Nie,Shengliang Zhong
A new hybrid membrane was prepared by a facile method based on a highly luminescent lanthanide coordination polymer and agarose. The soft membrane was characterized by FT-IR, PXRD, SEM and luminescence. It is found that the soft membrane is a highly selective and sensitive sensor, among 19 metal ion solutions of Fe 3+ , Mg 2+ , Li + , Ca 2+ , Zn 2+ , Cu 2+ , Ba 2+ , Mn 2+ , Ru 3+ , Cr 3+ , Ag + , Sr 2+ , Cd 2+ , Na + , Ni 2+ , Pb 2+ , Fe 2+ , Hg 2+ and Ca 2+ , only Fe 3+ quench the luminescence. The sensing results can be distinguished by the naked eye in daylight or by irradiation of a portable UV light at the scene. Mechanism studies reveal the sensing is due to the decomposition of the coordination polymer 1 which induced by slow permeation of Fe 3+ . Further studies found anions of BO 3 − , CO 3 2 − , H 2 PO 4 − , Br − , Cl − , ClO 4 − , H 2 PO 4 − , I − , IO 3 − and NO 3 − will not quench the luminescence of the hybrid membrane, which imply that other anions in water would not disturb the detection result.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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