Materials Science & Engineering C-Materials for Biological Applications
·
2013
·
IF 2.404
Wanyun Liu,Junchao Wei,Ping Huo,Yunhua Lu,Yiwang Chen,Yen Wei
In this work, PEG–PLLA electrospun fibers were developed as a new controlled release system for macrolide antibiotic drug brefeldin A (BFA). SEM and XRD analyses of the BFA-loaded PEG–PLLA fibers revealed that the average diameter of fibers was below 950 nm with smooth surfaces, and the drug was well incorporated into the fibers in amorphous form. The release profiles of BFA in PBS were measured by HPLC, demonstrating that the controlled release of BFA could be gained for long time. The in vitro antitumor activity against human liver carcinoma HepG2 cells of the fibers containing 3%, 6%, 9%, 12% and 15% BFA were examined by MTT method, and the results showed that cell growth inhibition rates at 72 h were 64%, 77%, 80%, 81% and 85%, respectively. These results strongly suggested that the BFA/PEG–PLLA fibers had an effect of controlled release of BFA and were suitable for postoperative chemotherapy of cancers.
研究领域:
药学
·
肿瘤学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Bowen Yu,Lu Meng,Sirui Fu,Zhiyu Zhao,Yuhang Liu,Ke Wang,Qiang Fu
The applications of Polylactide (PLA) microspheres in biomedical areas are greatly determined by the size, morphology and internal structure. Taking advantage of the formation of stereocomplex (SC) crystallites between poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA), we propose a facile strategy to prepare PLA microspheres with tunable morphology and crystalline structure by compounding PLLA and PDLA. With increasing PDLA content, the crystallinity of SC-PLA rose gradually until the ratio of PLLA and PDLA reached 1:1 and then fell. Correspondingly, the morphology of the microspheres were varied (smooth, porous, golf-ball like, guava like) and higher crystallinity of SC-PLA would lead to a more coarse and porous structure. Finally, three typical kinds of Rifampicin-loaded microspheres with different ratio of PLLA and PDLA (7:3, 3:7, 10:0, sorted by porosity from high to low) were prepared and the release behavior was compared. At 30 h, the cumulative release of 7:3, 3:7 and 10:0 microspheres were 32.6%, 17.8% and 6.0% respectively, indicating that the release profiles were generally determined by the porosity of the microspheres. Our findings not only provide a new strategy to prepare PLA microspheres with controllable morphology but offer additional possibilities for the applications of SC-PLA products in biomedical area.
研究领域:
生物医学工程
·
药学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Xi Cui,Yizhu Shan,Jiaxuan Li,Meng Xiao,Yuan Xi,Jianying Ji,Engui Wang,Baokun Zhang,Lingling Xu,Mingzhu Zhang,Zhou Li,Yingzi Zhang
Prevention of aseptic loosening, the main reason for secondary surgery of bone implants, is of great crucial clinical importance. As a promising bioactive material to promote bone regeneration, the piezoelectric materials for preventing aseptic loosening has not been reported. Here, a surface coating is presented for implants based on piezoelectric poly(L-lactic acid) (PLLA). The coating ingeniously utilizes zoledronic acid (ZA), a drug used to treat osteoporosis, as a nucleating agent to enhance the piezoelectricity of PLLA. The coating can not only provide a continuous porous piezoelectric environment to stimulate osteogenic differentiation without intervention, but also effectively inhibit the osteolytic activity of osteoclasts through the slow release of ZA, achieving long-term effectiveness with a single implantation. The rat femur cavity implantation experiment suggests that, the PLLA/ZA coating can rapidly initiate osseointegration of the implant and effectively mitigate the risk of aseptic loosening, even in the presence of metal submicroscopic particles that induce aseptic loosening. This strategy of piezo-enhanced PLLA drug-loaded coating may provide a new idea for the surface modification of bone implants.
研究领域:
生物医学工程
·
材料科学
·
骨科
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2012
·
IF 3.35
Cheng Zhang,Guodong Li,Yihan Wang,Fuying Cui,Jien Zhang,Qingshan Huang
In this work, 5-fluorouracil-loaded- poly( l -lactic)-polyethylene glycol/polyethylene glycol (5-FU-loaded-PLLA–PEG/PEG) nanoparticles were prepared using a novel reverse emulsion-solution enhanced dispersion by supercritical fluids (reverse emulsion-SEDS) technique in an effort to obtain an efficient drug delivery system . In the experiment, 5-FU and PEG were dissolved in water PLLA–PEG was dissolved in organic solution, the aqueous solution was added dropwise to the organic solution under magnetic stirring, a reverse emulsion was immediately formed. The reverse emulsion was dried by a SEDS process so that 5-FU-loaded-PLLA–PEG/PEG nanoparticles (5-FU-NPs) were obtained. The particle size, size distribution, surface morphology, and physical and chemical properties of the 5-FU-NPs were investigated by laser diffraction particle size analysis, scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and thermogravimetric analysis (TGA). The drug encapsulation efficiency (EE), drug loading (DL), in vitro release profile and pharmacokinetics of 5-FU-NPs in rat plasma were investigated by high-performance liquid chromatography (HPLC). The in vivo tumor inhibition effect, increase in lifespan and hepatotoxicity of placebo-NPs, 5-FU and 5-FU-NPs were determined using H22 tumor-bearing ICR mice. These results collectively suggest that 5-FU-NPs prepared using SEDS have potential anti-tumor applications as a controlled drug release dosage form without harmful drug toxicity .
研究领域:
药剂学
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生物医学工程
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Haiyan Yao,Jiaolong Wang,Yunyun Deng,Zhihua Li,Junchao Wei
Insufficient bone regeneration and bacterial infection are two major concerns of bone repair materials. Poly-L-lactic acid (PLLA) have been widely used in bone tissue engineering (BTE), however, lack of osteogenic and antibacterial properties have greatly limit its clinical application. Herein, PLLA membrane was firstly treated with polydopamine (PDA), and then modified with ε-polylysine (ε-PL) and alginate (ALG) via layer-by-layer method. The (ε-PL/ALG)n composite layer coated PLLA ( [email protected] (ε-PL/ALG)n) could facilitates the adhesion and osteoblast differentiation of MC3T3-E1 cells. Furthermore, [email protected] (ε-PL/ALG) n presents an effective antibacterial efficacy against S. aureus and E. coli , and the bacterial survival rates of S. aureus and E. coli on [email protected] (ε-PL/ALG) 10 were 21.5 ± 3.5 % and 13 ± 2.1 %, respectively. This work provides a promising method to design PLLA materials with osteogenic and antibacterial activity simultaneously. Furthermore, the method is also an optional choice to construct multifunctional coatings on the other substrate.
研究领域:
生物医学工程
·
材料科学
·
组织工程
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产品关联
DOI
PubMed
高影响因子
Journal of Engineered Fibers and Fabrics
·
2021
·
IF 1.573
Qijian Niu,Lili Ma,Junxia Guo,Xiaoping Yang
In this study, a temperature responsive PGS/PLLA@PNIPAM core-shell nanofiber membrane was prepared by combining electrospinning with surface ATRP grafting polymer technology, in which the core layer of PGS/PLLA nanofiber was prepared by electrospinning, and then the shell layer of temperature responsive PNIPAM polymer was grafted on the nanofiber surface by ATRP reaction. In the experiment, a macromolecule-initiator PGS-Br was prepared and characterized by FTIR and 1H NMR. The surface morphology, composition, element of the core-shell nanofiber membrane were characterized by SEM, FTIR, and XPS. In addition, it is worth noting that the core-shell nanofiber membrane can respond to temperature in drug release tests.
研究领域:
材料科学
·
高分子化学
·
纳米技术
·
药物传递系统
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产品关联
DOI
高影响因子
Journal of Advanced Research
·
2020
·
IF 6.992
Wenjing Yang,Yancheng Zhong,Chongxian He,Shuping Peng,Youwen Yang,Fangwei Qi,Pei Feng,Cijun Shuai
Cell responses and mechanical properties are vital for scaffold in bone regeneration. Fe 3 O 4 nanoparticles with excellent magnetism can provide magnetic stimulation for cell growth, while graphene oxide (GO) nanosheets are commonly used as reinforcement phases due to their high strength. However, Fe 3 O 4 or GO is tended to agglomerate in matrix. In present study, a novel co-dispersed Fe 3 O 4 -GO nanosystem was constructed through electrostatic self-assembly of positively charged Fe 3 O 4 ( p Fe 3 O 4 ) on negatively charged GO nanosheets. In the nanosystem, p Fe 3 O 4 nanoparticles and GO nanosheets support each other, which effectively alleviates the π-π stacking between GO nanosheets and magnetic attraction between p Fe 3 O 4 nanoparticles. Subsequently, the nanosystem was incorporated into poly L -lactic acid (PLLA) scaffolds fabricated using selective laser sintering. The results confirmed that the p Fe 3 O 4 -GO nanosystem exhibited a synergistic enhancement effect on stimulating cell responses by integrating the capturing effect of GO and the magnetic simulation effect of p Fe 3 O 4 . The activity, proliferation and differentiation of cells grown on scaffolds were significantly enhanced. Moreover, the nanosystem also exhibited a synergistic enhancement effect on mechanical properties of scaffolds, since the p Fe 3 O 4 loaded on GO improved the efficiency of stress transfer in matrix. The tensile stress and compressive strength of scaffolds were increased by 67.1% and 132%, respectively. In addition, the nanosystem improved the degradation capability and hydrophilicity of scaffolds.
研究领域:
生物医学工程
·
纳米材料科学
·
组织工程
·
材料力学
·
表面与界面科学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2014
·
IF 4.287
Zhongling Wei,Peng Tian,Xuanyong Liu,Bangxin Zhou
Biodegradable magnesium and its alloys have attracted much attention, as they have been used as cardiovascular stents recently because of their biodegradation after implantation. However, their corrosion resistance, hemocompatibility and surface biocompatibility are needed for practical applications. In this work, heparinization of the plasma electrolytic oxidation/poly( l -lactic acid) (PEO/PLLA) composite coating on biodegradable AZ31 alloy was achieved by the strong adhesion of mussel-inspired polydopamine (PDAM). The corrosion resistance of the coated substrates was evaluated in simulated body fluid. In particular, the hemolysis ratio and platelet adhesion tests were conducted to evaluate the hemocompatibility of the composite coatings. The in vitro cytotoxicity of the composite coatings was evaluated with human umbilical vein endothelial cells (HUVECs). The adhesion and proliferation of HUVECs and human umbilical artery smooth muscle cells (HUASMCs) directly incubated on the composite coatings were also investigated. The results showed that although PDAM modification and further heparinization reduced the corrosion resistance of the PEO/PLLA composite coating, the protection of the coating for the substrate was mainly maintained. Moreover, PDAM modification and further heparinization significantly suppressed the adhesion of platelets and had little influence on sustaining a low hemolysis ratio thus resulting in good surface hemocompatibility of the composite coating. The in vitro cell test demonstrated that none of the composite coatings presented obvious cytotoxicity. Significantly, after surface heparinization, the composite coating became more suitable for HUVEC growth and simultaneously inhibited HUASMC growth. The results show that further modification of the PEO/PLLA composite coating on biodegradable magnesium alloy is a promising method to obtain good surface hemocompatibility for anticoagulation and to regulate the cell fate for fast re-endothelialization while sustaining the corrosion resistance of biodegradable magnesium-based cardiovascular stents.
研究领域:
生物医学工程
·
材料科学
·
心血管医学
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产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Zhao Miaoran,Chang Shuhua,Wang Yunpeng,Cao Jun,Pu Yuji,He Bin,Pan Shengsheng
Injectable facial fillers such as Sculptra® stimulate collagen regeneration to fill wrinkles; however, the collagen regeneration is not satisfactory due to the slow emergence of filling effect. In this study, we designed a regenerative dermal filler to provide both immediate and long-lasting filling effects. A hydrogel matrix composed of crosslinked hyaluronic acid (HA) and collagen was engineered to encapsulate porous poly(L-lactide) (PLLA) microspheres and tranexamic acid (TXA). The hydrogel matrix was administered via intradermal injection to achieve wrinkle filling. TXA is released to exert skin-whitening effects, while the porous PLLA microspheres and their degradation product, lactic acid, continuously stimulate collagen regeneration over an extended period. Facial volume increased immediately following hydrogel injection. Large amounts of new Type I and Type III collagens are generated. The porous structure of PLLA microspheres facilitated the “penetrating growth” of collagen fibers, which effectively filled facial depressions and smoothed wrinkles. Overall, the HA/collagen composite hydrogel filler exhibited excellent aesthetic effects.
研究领域:
生物材料学
·
医学美容
·
组织工程学
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产品关联
DOI
PubMed
高影响因子
POLYMER TESTING
·
2021
·
IF 4.282
Jianqi Yao,Faliang Luo,Jie Mao,Yuting Li,Yongdong Liu,Xiaolei Sun
Different crystal planes of nanocrystalline often displayed diverse physical and chemical properties. In this paper, the effects of nano-ZnO with two kinds of crystal planes on crystallization, thermal stability and mechanical properties of PLLA were investigated. The results show that the (1010) planes with no-polar and low surface energy increased the chain mobility of PLLA chain, showed a plasticizing effect; the glass transition temperature, melt and cold crystallization temperature decreased by 12 °C, 10 °C and 12 °C, respectively. The size of spherulites increased and the number of spherulites decreased, the crystal form changed from mixed crystal form α, α′ to unique α crystal form. However, the (0002) planes with polar and high surface energy has highly nucleating effective for PLLA, the crystallization temperature increased to 106.41 °C, the cold crystallization peak disappear. The size of spherulites decreased and the number of spherulites increased. Moreover, the (0002) planes increases the elongation at break of PLLA to 20.34% but the (1010) planes reduces to 7.49%. Their thermo-gravimetric analysis results showed the similar trend. Our results indicate that the interface wettability and compatibility between crystal planes and PLLA, which was caused by the polarity and surface energy of (1010) or (0002) planes played key role in improving the performance of polymers.
研究领域:
材料科学
·
高分子科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Yongjing Xu,Bo Liu,Liming Zou,Chenkai Sun,Wengang Li
In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method. PLLA and dichloromethane/ethanol (CH 2 Cl 2 /EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning. The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL. The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA. The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°–19°, 109%–482%, 1945–2517 g m −2 day −1 and 4–14%, respectively. The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA. The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats. Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering.
研究领域:
生物材料学
·
组织工程学
·
医学材料
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 32
·18614-18623
·
IF 3.119
Biyun Li,Feng Xiong,Bo Yao,Qian Du,Jun Cao,Jiangang Qu,Wei Feng,Huihua Yuan
Electrospun poly(L)-lactide (PLLA) ultrafine fibers are a biodegradable and biocompatible scaffold, widely used in tissue engineering applications. Unfortunately, these scaffolds have some limitations related to the absence of bioactivity and antibacterial capacity. In this study, dopamine-functionalized reduced graphene oxide (rGO)/PLLA composite nanofibers were fabricated via electrospinning. The morphology and the physicochemical and biological properties of the composite nanofibers were investigated. The results indicate that incorporating rGO improves the hydrophilic, mechanical, and biocompatibility properties of PLLA nanofibers. Tetracycline hydrochloride (TC)-loaded rGO/PLLA composite nanofibers showed better controlled drug release profiles compared to GO/PLLA and PLLA nanofibrous scaffolds. Drug-loaded nanofibrous scaffolds showed significantly improved antibacterial activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). Additionally, rGO/PLLA composite nanofibers exhibited enhanced cytocompatibility. Thus, it can be concluded that rGO/PLLA composite nanofibers allow the development of multifunctional scaffolds for use in biomedical applications.
研究领域:
生物材料
·
组织工程
·
纳米技术
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）