ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Bofan Li,Xiaoming Zhou,Hongxing Liu,Huaping Deng,Ru Huang,Da Xing
Antibiotic resistance, the ability of some bacteria to resist antibiotic drugs, has been a major global health burden due to the extensive use of antibiotic agents. Antibiotic resistance is encoded via particular genes; hence the specific detection of these genes is necessary for diagnosis and treatment of antibiotic resistant cases. Conventional methods for monitoring antibiotic resistance genes require the sample to be transported to a central laboratory for tedious and sophisticated tests, which is grueling and time-consuming. We developed a paper-based chip, integrated with loop-mediated isothermal amplification (LAMP) and the “light switch” molecule [Ru(phen)2dppz]2+, to conduct turn-on fluorescent detection of antibiotic resistance genes. In this assay, the amplification reagents can be embedded into test spots of the chip in advance, thus simplifying the detection procedure. [Ru(phen)2dppz]2+ was applied to intercalate into amplicons for product analysis, enabling this assay to be operated in a wash-free format. The paper-based detection device exhibited a limit of detection (LOD) as few as 100 copies for antibiotic resistance genes. Meanwhile, it could detect antibiotic resistance genes from various bacteria. Noticeably, the approach can be applied to other genes besides antibiotic resistance genes by simply changing the LAMP primers. Therefore, this paper-based chip has the potential for point-of-care (POC) applications to detect various gene samples, especially in resource-limited conditions.
研究领域:
生物技术
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分子生物学
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医学诊断
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分析化学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2024
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IF 3.8
Sun Rengang,Zhou Yadong,Fang Yunzhu,Qin Yirui,Zheng Yekai,Jiang Li
The harm and impact of the COVID-19 pandemic have highlighted the importance of fast, sensitive, and cost-effective virus detection methods. In this study, we developed a DNA aptamer sensor using nanoparticle-enhanced surface plasmon resonance imaging (SPRi) technology to achieve efficient labeling-free detection of SARS-CoV-2 S protein. We used the same DNA aptamer to modify the surface of the SPRi sensor chip and gold nanoparticles (AuNPs), respectively, for capturing target analytes and amplifying signals, achieving ideal results while greatly reducing costs and simplifying the preparation process. The SPRi sensing method exhibits a good linear relationship ( R 2 = 0.9926) in the concentration range of 1–20 nM before adding AuNPs to amplify the signal, with a limit of detection (LOD) of 0.32 nM. After amplifying the signal, there is a good linear relationship ( R 2 = 0.9829) between the concentration range of 25–1000 pM, with a LOD of 5.99 pM. The simulation results also verified the effectiveness of AuNPs in improving SPRi signal response. The SPRi sensor has the advantage of short detection time and can complete the detection within 10 min. In addition, the specificity and repeatability of this method can achieve excellent results. This is the first study to simultaneously capture a viral marker protein and amplify the signal using polyadenylic acid (polyA)–modified DNA aptamers on the SPR platform. This scheme can be used as a fast and inexpensive detection method for diagnosis at the point of care (POC) to combat current and future epidemics caused by the virus.
研究领域:
生物传感器
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病毒学
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纳米技术
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
PHYSICS OF FLUIDS
·
2022
·Vol. 34
·期 2
·
IF 4.98
Gong Yanli,Zhang Cheng,Weng Xuan,Peng Bei,Jiang Hai
Microfluidic devices have been demonstrated to be able to concentrate low-abundance analytes and to be integrated in point of care (POC) devices for the purpose of reducing equipment size and cost; however, the complications involved in the fabrication of microfluidic chips or microelectrodes can be challenging. Here, we present a simple and effective microfluidic device monolithically integrated with liquid metal-based microelectrodes for the transportation and concentration of molecules in dilute solutions. Numerical and experimental studies were combined to validate and optimize the performance of the presented co-designing microfluidic chip featured by a double T-shaped manifold microchannel, in which the electrokinetically induced pressure-driven flow and concentration process were fully characterized. The microfluidic chip was used in a fluorimetric assay based on fluorescence resonance energy transfer between the fluorophore labeled aptamer and molybdenum disulfide nanosheets for the detection of cortisol, a stress biomarker of humans. Both experimental and numerical results show that a concentration factor up to 83-fold can be achieved within 5 min. By avoiding cumbersome fabrication processes and mechanical equipment, the proposed co-designing concentrating strategy provides insights for the fabrication of compact microfluidic chips that facilitate the miniaturization of POC devices with enhanced detection limit and sensitivity.
研究领域:
微流控技术
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生物医学工程
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分析化学
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即时诊断
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Zhiying Wang,Yang Wang,Long Lin,Tao Wu,Zhenzhen Zhao,Binwu Ying,Lingqian Chang
Tuberculosis (TB) remains the high-risk infectious pathogen that caused global pandemic and high mortality, particularly in the areas lack in health resources. Clinical TB screening and diagnosis are so far mainly conducted on limited types of commercial platforms, which are expensive and require skilled personnel. In this work, we introduced a low-cost and portable finger-driven microfluidic chip (named Fd-MC) based on recombinase polymerase amplification (RPA) for rapid on-site detection of TB. After injection of the pre-treated sample solution, the pre-packaged buffer was driven by the pressure generated by the finger-actuated operation to accomplish sequential processes of diagnosis in a fully isolated microchannel . An in-situ fluorescence strategy based on FAM-probe was implemented for on-chip results read-out though a hand-held UV lamp. Hence, the Fd-MC proved unique advantageous for avoiding the risk of infection or environmental contamination. In addition, the Fd-MC was designed for multiplexed detection, which is able to not only identify TB/non-TB infection, but also differentiate between human Mycobacterium tuberculosis and Mycobacterium bovis . The platform was verified in 37 clinical samples, statistically with 100% specificity and 95.2% sensitivity as compared to commercial real-time RPA. Overall, the proposed platform eliminates the need on external pumps and skilled personnel, holding promise to POC testing in the resource-limited area.
研究领域:
生物医学工程
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微流控技术
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分子诊断
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传染病
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即时检测
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全球健康
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Shuofeng Li,Fangfang Wang,Chenhuan Wang,Lin Hao,Ningzhao Shang,Pengbo Zhang,Yawen Zhang,Nuo Xiao,Yaai Kang,Jin Liu,Shutao Gao,Chun Wang,Zhi Wang,Qiuhua Wu
Single-atom nanozyme (SAZ) with peroxidase-like activity has attracted great attention in point-of-care (POC) diagnostic, but the use of unstable H 2 O 2 in peroxidase catalytic reactions often leads to low detection accuracy. Thus, developing SAZ with high oxidase (OD)-like activity to construct RNA sensing systems independent of H 2 O 2 is imperative for improving accuracy. Herein, a novel strategy to fabricate boron-nitrogen co-doped zinc SAZ (ZnBNC-SAZ) with excellent OD-like activity by carbonizing Zn based zeolitic boron imidazolate frameworks is reported. The electron deficient B in imidazolate ligand can form Zn─N─B bond in situ during high temperature pyrolysis, which can regulate the electronic structure of Zn and further upshift the d-band center of Zn to improve the OD-like activity. With ZnBNC-SAZ as signal generator, a new method for sensitive RNA detection is developed by combining CRISPR/ cas13 system with terminal deoxynucleotidyl transferase-induced DNA extension reaction. The limits of detection for RNAs are as low as 20 aM. The proposed biosensor is adaptable to a lateral-flow-based readout and is universally applicable for sensing various RNAs by programming the guide RNA. Importantly, the proposed biosensor can monitor the cellular differentiation and identify patients with cervical carcinoma, showing great potential for application in facile POC diagnosis.
研究领域:
纳米技术
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生物化学
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分子生物学
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医学诊断
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生物医学工程
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Shuqing Huang,Zhenping Liu,Yu Yan,Jiamei Chen,Ruizhi Yang,Qiuju Huang,Mingliang Jin,Lingling Shui
C-reactive protein (CRP) is one of the most sensitive acute-phase reactants, which is an early stage indicator of cardiovascular disease and infectious inflammation in clinic. However, it is still challenging to accurately quantification the trace content of CRP molecules in plasma samples. In this work, we propose an ultrasensitive electrochemical CRP aptasensor based on rhomboid dodecahedra carbonized-ZIF 67 loaded with gold nanoparticle modified by aptamer . Aptamer biomolecules are binded to AuNPs via Au-thiol bonds for selectively capturing CRPs. The ultrasensitivity is achieved based on triple signal enhancing strategy: enhancing the specific surface area via the rhomboid dodecahedra structure of ZIF 67 , increasing the conductivity via carbonization of ZIF 67 , and multiplying the number of probe molecules via an enzyme catalyzed reaction . Experimental parameters, including the volume of C-ZIF 67 dispersion, electrodeposition time of AuNPs, incubation time of aptamer-CRP and aptamer-CRP concentration, are systemically investigated and optimized. Under optimal conditions, the proposed biosensor shows excellent sensing performance with the limit of detection (LOD) of 0.44 pg mL −1 (S/N = 3), and a broad linear dynamic range of 10 pg mL −1 ‒ 10 μg mL −1 within the total readout time of 5 min. This work provides an effective electrochemical biosensor for CRP assay in plasma, being highly potential for applications in bioanalysis and point-of-care (POC) clinical diagnosis.
研究领域:
生物化学
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分析化学
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生物医学工程
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临床诊断
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 10
·818
·
IF 5.743
Jiali Huang,Xu Li,Mingzhen Xiu,Kang Huang,Kang Cui,Jing Zhang,Shenguang Ge,Shiji Hao,Jinghua Yu,Yizhong Huang
Nowadays, developing a cost-effective, easy-to-operate, and efficient signal amplification platform is of important to microfluidic paper-based analytical devices (μPAD) for end-use markets of point-of-care (POC) assay applications. Herein, an ultrasensitive, paper-based photoelectrochemical (PEC) bioassay platform is constructed by in situ grown ZnO/ZnIn2S4heterojunctions onto paper fibers, which acted as photoactive signal amplification probes for enhancing the sensitivity of antibodies-based diagnostic assays, for the sensitive detection of alpha-fetoprotein (AFP) targets. The crystalline flake-like ZnIn2S4composited with hexagonal nanorods (NRs) morphology of ZnO is an in situ grown, at the first time, onto cellulose fibers surface supported with Au nanoparticle (Au NP) modification to improve conductivity of the device working zone. The obtained composites on paper fibers are implemented as a flexible paper-based photoelectrode to realize remarkable performance of the fabricatedμPAD, resulting from the enhanced PEC activity of heterojunctions with effective electron-hole pair separation for accelerating photoelectric conversion efficiency of the sensing process under light irradiation. Once the target AFP was introduced into the biosensing interface assistant, with a specific recognition interaction of AFP antibody, a drastically photocurrent response was generated, in view of the apparent steric effects. With the concentration increase of AFP targets, more immune conjugates could be confined onto the biosensing interface, eventually leading to the quantitative decrease of photocurrent intensity. Combined with an ingenious origami design and permitting the hydrophobic/hydrophilic conversion procedure in the bioassay process, the ultrasensitive PEC detection of AFP targets was realized. Under the optimized conditions, the level of AFP could be sensitively tracked by the preparedμPAD with a liner range from 0.1 to 100 ng mL−1and limit of detection of 0.03 ng mL−1. This work provides a great potential application for highly selective and sensitive POC testing of AFP, and finally, developments for clinical disease diagnosis.Keywords:μPAD;photoelectrochemical;alpha-fetoprotein;point-of-care assay;immunosensing
研究领域:
生物医学工程
·
分析化学
·
即时诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）