Science China-Chemistry
·
2023
·
IF 9.6
Tang Xianqing,Chen Jinghuang,Zhang Mengqian,Sun Jian,Yang Xiurong
Alkaline phosphatase (ALP) activity and pyrophosphate ion (PPi) levels are remarkable for the human body functions such as signal transduction pathways and metabolism. Current quantitative methods mainly focus on developing complicated organic substrates or employing unstable metal ions as signal-regulated medium. Herein, we have developed a facile hydrothermal method for preparing FeWO 4 nanomaterials with intrinsic peroxidase-like activity and further confirmed that such a catalytic activity could be significantly enhanced by adjusting the size and oxygen vacancy content. More encouragingly, PPi can easily inhibit the catalytic activity of FeWO 4 , whereas orthophosphate ions (Pi) cannot. Therefore, we constructed an FeWO 4 -based colorimetric assay for sensing PPi by means of the classical 3,3′,5,5′-tetramethylbenzidine-peroxidase chromogenic reaction. A facile and reliable ALP activity assay was also designed and developed because of the logical regulation of the peroxidase-like activity of FeWO 4 through the ALP-catalyzed hydrolysis of PPi into Pi. Based on the clear mechanism and mimetic-enzyme FeWO 4 -catalyzed amplification, the sensing system exhibited excellent performance and was able to evaluate ALP activity in real serum samples and screen for potential ALP inhibitors. The proposed mimetic enzyme-involved colorimetric assay provides an alternative pathway, and FeWO 4 nanomaterials with excellent performance have great potential for further biosensing and biomedical applications.
研究领域:
纳米技术
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生物化学
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催化
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分析化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Fenghua Geng,Changpeng Zou,Jinhua Liu,Qianchen Zhang,Xueying Guo,Yunchang Fan,Haidong Yu,Sheng Yang,Zhipeng Liu,Lin Li
As an important biomarker, alkaline phosphatase (ALP) is one of the most commonly assayed enzymes in clinical practice. Here a novel turn-on fluorescent nanoswitch for ALP assay was suggested. The nanoswitch was easily constructed via two high-affinity ligands between GSH and Al 3+ and PPi and Al 3+ based on the difference in the affinity. The primary fluorescence of as-prepared GSH capped Cu nanoclusters (NCs) turned on first upon the Al 3+ addition due to the Al 3+ induced aggregation induced emission (AIE) enhancement based on the high affinity of GSH and Al 3+ . The presence of PPi then made Al 3+ desorb from the surface of GSH capped Cu NCs due to the higher affinity of PPi and Al 3+ . As a result, the fluorescence of the Cu NCs was quenched. ALP could hydrolyze PPi into phosphate , destroying the PPi-Al 3+ complex and releasing Al 3+ . Thus, the Al 3+ binds to GSH again and the fluorescence was restored. The nanoswitch was demonstrated to be sensitive and selective for ALP assay and was successfully used for the ALP assay in the human serum.
研究领域:
生物化学
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医学检测
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纳米技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
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IF 5.667
Jidong Zhang,Mengyao She,Jie Li,Cuiling Wang,Shen Wang,Ping Liu,Shengyong Zhang,Jianli Li
A series of naphthalene carbohydrazone based fluorescent chemosensors L 1 -L 4 were developed for the detection of Zn 2+ by significant fluorescence enhancement. The binding ratio of the L 1 -Zn 2+ complex was determined by a Job’s plot to be 1:1 and confirmed by ESI–MS studies. The corresponding of the L 1 -Zn 2+ ensemble was elucidated through X-ray crystallography along with spectroscopic studies. Furthermore, the L X -Zn 2+ (X = 1–2) complexes can specifically detect pyrophosphate (PP i ) in HEPES buffer solution through fluorescence quenching. The detection limit of L X -Zn 2+ (X = 1–2) for PP i sensing were calculated to be as low as 2.54 ppb and 0.94 ppb, respectively. In addition, due to their good cell membrane permeability and low cytotoxicity, L 1 -L 4 have been applied to sequentially detect Zn 2+ and PP i in living cells. More importantly, the electronic effect of substitute groups on fluorescence signaling have been studied by substituent properties ranging from electron-donating to electron-withdrawing, which sets up a promising strategy for the rational design of fluorescent sensors.
研究领域:
分析化学
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生物化学
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材料科学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Shengling Li,Duanlin Cao,Xianjiao Meng,Zhiyong Hu,Zhichun Li,Changchun Yuan,Tao Zhou,Xinghua Han,Wenbing Ma
In this study, a highly selective fluorescent sensor ( E )-2-((2-(benzo[ d ]thiazol-2-yl)quinolin-8-yl)oxy)- N ′ -((7-(diethylamino)-2-oxo-2 H -chromen-3-yl)methylene)acetohydrazide ( TQC ) was synthesized from 2-methylquinolin-8-ol and 4-(diethylamino)-2-hydroxybenzaldehyde and its structure was characterized by 1 H NMR, 13 C NMR, ESI-HR-MS and density functional theory (DFT) calculation. Sensor TQC showed an obvious “on-off-on” fluorescence response to Cu 2+ and PPi in a DMSO/HEPES (3:2 v/v, pH = 7.4) buffer system. The detection limits of sensor TQC were 0.06 μM to Cu 2+ and 0.01 μM to PPi. In addition, sensor TQC showed a 1:1 binding stoichiometry to Cu 2+ and TQC- Cu 2+ complex showed a 2:1 binding stoichiometry to PPi. The optimum pH range of sensor TQC and TQC- Cu 2+ was 3–8. Further studies demonstrated that sensor TQC could be made into test paper strips for the qualitative of Cu 2+ and PPi and showed sequentially “on-off-on” fluorescent bio-imaging of Cu 2+ and PPi in HeLa cells.
研究领域:
分析化学
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生物医学
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材料科学
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DOI
PubMed
高影响因子
CHEMBIOCHEM
·
2022
·Vol. 23
·期 20
·e202200413
·
IF 3.461
Lingxia Qin,Xinxin Ren,Kaiyue Hu,Di Wu,Zhiyong Guo,Sui Wang,Linwen Jiang,Yufang Hu
Graphical An electrochemical biosensor based on the supramolecular host-guest recognition between cucurbit[7]uril (CB[7]) and L -phenylalanine-Cu(II) complex is reported for pyrophosphate (PPi) and alkaline phosphatase (ALP) analysis. We report an electrochemical biosensor based on the supramolecular host-guest recognition between cucurbit[7]uril (CB[7]) and L -phenylalanine-Cu(II) complex for pyrophosphate (PPi) and alkaline phosphatase (ALP) analysis. First, L -Phe-Cu(II) complex is simply synthesized by the complexation of Cu(II) (metal node) with L -Phe (bioorganic ligand), which can be immobilized onto CB[7] modified electrode via host-guest interaction of CB[7] and L -Phe. In this process, the signal of the complex-triggered electro-catalytic reduction of H 2 O 2 can be captured. Next, due to the strong chelation between PPi and Cu(II), a biosensing system of the model “PPi and Cu(II) premixing, then adding L -Phe” was designed and the platform was applied to PPi analysis by hampering the formation of L -Phe-Cu(II) complex. Along with ALP introduction, PPi can be hydrolyzed to orthophosphate (Pi), where abundant Cu(II) ions are released to form L -Phe-Cu(II) complex, which gives rise to the catalytic reaction of complex to H 2 O 2 reduction. The quantitative analysis of H 2 O 2 , PPi and ALP activity was achieved successfully and the detection of limits are 0.067 μM, 0.42 μM and 0.09 mU/mL ( S / N =3), respectively. With its high sensitivity and selectivity, cost-effectiveness, and simplicity, our analytical system has great potential to for use in diagnosis and treatment of ALP-related diseases.
研究领域:
分析化学
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生物化学
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电化学
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分子识别
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生物传感器
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2020
·
IF 3.306
Shengling Li,Duanlin Cao,Xianjiao Meng,Zhiyong Hu,Zhichun Li,Changchun Yuan,Tao Zhou,Xinghua Han,Wenbing Ma
In this study, a novel schiff base fluorescent probe (E)- N' -(3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methylbenzylidene)-7-(diethylamino)-2-oxo-2 H -chromene-3-carbohydrazide ( CHS ) was synthesized for sequential detection of Al 3+ and pyrophosphate ions (PPi) in a DMSO/HEPES (4:1 v/v, pH = 7.4) buffer system and characterized by 1 H NMR, 13 C NMR, ESI-HR-MS and DFT calculation. Its detection limit was determined to be 0.16 μM for Al 3+ and 0.18 μM for PPi in a pH range of 4∼8, respectively. The Job’ s plot showed that the stoichiometric ratio to Al 3+ was 1:1, and CHS -Al 3+ showed highly selective recognition of PPi. The sensor CHS could also recognize Al 3+ and PPi and exhibited fluorescence enhancement and quenching properties in HeLa cells.
研究领域:
分析化学
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生物分析
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分子探针
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细胞成像
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金属离子检测
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DOI
高影响因子
BIOCONJUGATE CHEMISTRY
·
2024
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IF 4
Shu Li,Shibo Song,Xiaojing Liu,Xingjiao Zhang,Xueya Liang,Xin Chang,Daijun Zhou,Jianting Han,Yaoyan Nie,Chen Guo,Xiaojun Yao,Min Chang,Yali Peng
Aberrant canonical NF-κB signaling has been implicated in diseases, such as autoimmune disorders and cancer. Direct disruption of the interaction of NEMO and IKKα/β has been developed as a novel way to inhibit the overactivation of NF-κB. Peptides are a potential solution for disrupting protein–protein interactions (PPIs); however, they typically suffer from poor stability in vivo and limited tissue penetration permeability, hampering their widespread use as new chemical biology tools and potential therapeutics. In this work, decafluorobiphenyl-cysteine SNAr chemistry, molecular modeling, and biological validation allowed the development of peptide PPI inhibitors. The resulting cyclic peptide specifically inhibited canonical NF-κB signaling in vitro and in vivo, and presented positive metabolic stability, anti-inflammatory effects, and low cytotoxicity. Importantly, our results also revealed that cyclic peptides had huge potential in acute lung injury (ALI) treatment, and confirmed the role of the decafluorobiphenyl-based cyclization strategy in enhancing the biological activity of peptide NEMO–IKKα/β inhibitors. Moreover, it provided a promising method for the development of peptide-PPI inhibitors.
研究领域:
药理学
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分子生物学
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炎症研究
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药物开发
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肽化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yixuan Yao,Fudong Zeng,Jinli Ma,Liangqiang Wu,Shuo Xing,Haiqin Yang,Yapeng Li,Jianshi Du,Qingbiao Yang,Yaoxian Li
As the second most abundant transition metal element in the human body, zinc ions play an important role in the normal growth and development of the human body. We have successfully synthesized a near-infrared fluorescent probe with FRET effect for the detection of Zn 2+ . Probe DR6G has good selectivity and anti-interference ability for Zn 2+ . When Zn 2+ is added to the probe DR6G solution, it responds completely within seconds, releasing red fluorescence with a detection limit of 2.02 × 10 −8 M. As the main product of ATP hydrolysis, PPi is indispensable in various metabolic activities in cells and the human body. Due to the strong binding ability of Zn 2+ and PPi, it is easy to form ZnPPi precipitation, so we added PPi to the solution to complete the Zn 2+ detection, and realized the continuous detection of PPi, and the detection limit was 2.06 × 10 −8 M. Since Zn 2+ and PPi play an important role in vivo, it is of great practical significance to design and synthesize a fluorescent probe that can continuously detect Zn 2+ and PPi. Biological experiments have shown that the probe DR6G has low cytotoxicity and can complete the detection of exogenous Zn 2+ and PPi in cells and living mice in vitro. Bacterial experiments have shown that the DR6G probe also has certain research value in the field of environmental monitoring and microbiology. Due to the constant variation of the fluorescence signals of Zn 2+ and PPi during detection, we designed the logic gate program. In practical applications, the probe DR6G can quantitatively detect Zn 2+ in zinc-containing oral liquids and qualitatively detect PPi in toothpaste.
研究领域:
生物化学
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分析化学
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医学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 36
·5502-5509
·
IF 5.047
Yifang Gao,Yuan Jiao,Huilin Zhang,Wenjing Lu,Yang Liu,Hui Han,Xiaojuan Gong,Lei Li,Shaomin Shuang,Chuan Dong
Herein, an easy and effective ratiometric fluorescent nanoprobe for the selective detection of Pb2+ and pyrophosphate (PPi) was developed based on label-free carbon dots (CDs). The CDs showed dual emission bands at 477 and 651 nm under a single-wavelength excitation (391 nm). Pb2+ and PPi were sequentially detected by the sensor with high reliability and accuracy via the exploitation of the fluorescence intensity ratio (F477/F651). The dual emission sensor exhibited high selectivity and excellent sensitivity for Pb2+ and PPi than for other analytes with detection limits as low as 0.055 μM and 0.089 μM, respectively. Moreover, the fluorescent CDs changed from pink to cyan and from cyan to pink and could be used as a fluorescent paper sensor for the visual detection of Pb2+ and PPi. To the best of our knowledge, this is the first report on the ratiometric fluorescence detection of Pb2+ and PPi by CDs; therefore, the as-synthesized CDs show great potential for application in the determination of Pb2+ and PPi in real samples.
研究领域:
分析化学
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生物传感
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环境监测
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材料科学
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荧光探针
✓ 已验证
DOI
高影响因子
Small
·
2023
·Vol. 19
·期 20
·2208167
·
IF 13.3
Mengya He,Xican Xu,Hongyu Wang,Qingyuan Wu,Linghao Zhang,Dongsheng Zhou,Yigang Tong,Xin Su,Huiyu Liu
Fluorescence-based PCR and other amplification methods have been used for SARS-CoV-2 diagnostics, however, it requires costly fluorescence detectors and probes limiting deploying large-scale screening. Here, a cut-price colorimetric method for SARS-CoV-2 RNA detection by iron manganese silicate nanozyme (IMSN) is established. IMSN catalyzes the oxidation of chromogenic substrates by its peroxidase (POD)-like activity, which is effectively inhibited by pyrophosphate ions (PPi). Due to the large number of PPi generated by amplification processes, SARS-CoV-2 RNA can be detected by a colorimetric readout visible to the naked eye, with the detection limit of 240 copies mL −1 . This conceptually new method has been successfully applied to correctly distinguish positive and negative oropharyngeal swab samples of COVID-19. Colorimetric assay provides a low-cost and instrumental-free solution for nucleic acid detection, which holds great potential for facilitating virus surveillance.
研究领域:
生物技术
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分子生物学
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病毒学
·
诊断方法
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jinyuan Hu,Xu Luo,Mogellah John Panga,Clara Appiah,Vladimir Retyunskiy,Lin Zhu,Ye Zhao
Zinc pyrithione (ZPT) is widely recognized for its beneficial properties as an antifouling, antibacterial, and antifungal agent. Despite its positive industrial contributions, ZPT has been proven to exhibit toxicity towards various ecosystems, particularly affecting marine life. However, there is still a dearth of comprehensive research on ZPT toxicity and its toxicological mechanism in reproductive systems of aquatic organisms. In our study, we conducted a thorough analysis and unveiled a multitude of abnormalities in zebrafish sperm and testicular tissue caused by ZPT exposure, including a dose-dependent diminishing of testosterone levels, various sperm deformities, decreased sperm concentration and motility, and ROS-induced testicular tissue DNA damage. In addition, our study suggested that ZPT-induced testicular damage is associated with heightened oxidative stress, apoptosis, and possible hyperpolarization of the mitochondrial membrane. Through RNA-seq analysis, a total of 409 DEGs associated with ZPT-induced testicular injury were identified, and the hub gene was determined using a protein-protein interaction network (PPI). The genes and pathways uncovered in this study point to potential mechanisms of ZPT exposure on sperm and testicular injury in zebrafish.
研究领域:
环境毒理学
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生殖生物学
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水生毒理学
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分子生物学
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DOI
PubMed
高影响因子
Frontiers in Immunology
·
2024
·
IF 5.7
Jijin Jiang,Yan Chen,Yue Su,Li Zhang,Hao Qian,Xinmiao Song,Jin-Fu Xu
Background Sepsis is an uncontrolled reaction to infection that causes severe organ dysfunction and is a primary cause of ARDS. Patients suffering both sepsis and ARDS have a poor prognosis and high mortality. However, the mechanisms behind their simultaneous occurrence are unclear. Methods We acquired sepsis and ARDS datasets from GEO and Arrayexpress databases and screened hub genes by WGCNA and machine learning algorithm. For diagnosis and prognosis, ROC curve and survival analysis were used. We performed GO, KEGG, GSEA, immune cell infiltration, drug prediction, molecular docking, transcription factor prediction, and constructed PPI and ceRNA networks to explore these genes and the common mechanisms of sepsis and ARDS. Single-cell data analysis compared immune cell profiles and hub gene localization. Finally, RT-qPCR and H&E staining confirmed the reliability of hub genes using PBMCs samples and mouse models. Results We identified 242 common differentially expressed genes in sepsis and ARDS. WGCNA analysis showed that the turquoise module in GSE95233 is strongly linked to sepsis occurrence and poor prognosis, while the black module in GSE10474 is associated with ARDS. Using WGCNA and three machine learning methods (LASSO, random forest and Boruta), we identified three key genes CX3CR1, PID1 and PTGDS. Models built with them showed high AUC values in ROC curve evaluations and were validated by external datasets, accurately predicting the occurrence and mortality. We further explored the immunological landscape of these genes using immune infiltration and single-cell analysis. Then, the ceRNA, predicted drugs and molecular docking were analyzed. Ultimately, we demonstrated that these genes are expressed differently in human and mouse samples with sepsis and ARDS. Conclusion This study identified three molecular signatures (CX3CR1, PID1 and PTGDS) linked to the diagnosis and poor prognosis of sepsis and ARDS, validated by RT-qPCR and H&E staining in both patient and mouse samples. This research may be valuable for identifying shared biological mechanisms and potential treatment targets for both diseases.
研究领域:
医学遗传学
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免疫学
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生物信息学
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分子生物学
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转化医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）