BIOLOGICAL CONTROL
·
2014
·
IF 1.873
Waseem Raza,Jun Yuan,Ning Ling,Qiwei Huang,Qirong Shen
The volatile organic compounds (VOCs) produced by antagonistic microbes have great antifungal potential against soil-borne fungal pathogens. The VOCs produced by Paenibacillus polymyxa strain WR-2 in the presence of root exudates and organic fertilizer were identified and their effects on the growth and spore germination of Fusarium oxysporum f. sp. niveum were evaluated. The VOCs produced by WR-2 inhibited the growth of F. oxysporum by 38%, 36% and 40% in agar medium, sterilized soil and natural soil, respectively. This inhibitory effect was increased to 60%, 58% and 64% with the addition of organic fertilizer in agar medium, sterilized soil and natural soil, respectively. The addition of root exudates did not affect the production of antifungal VOCs by WR-2. The VOCs produced by WR-2 completely inhibited the germination of F. oxysporum spores. Out of 42 identified VOCs, seven VOCs; benzothiazole, benzaldehyde, undecanal, dodecanal, hexadecanal, 2-tridecanone and phenol were found to inhibit the growth of F. oxysporum . The results of these experiments suggest another significance of using organic fertilizer as a carrier material with the biocontrol agents to control soil-borne fungal pathogens.
研究领域:
微生物学
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植物病理学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hao Liu,Min Huang,Shuxin Wei,Xiaowen Wang,Yaqin Zhao,Zhengyang Han,Xianfeng Ye,Zhoukun Li,Yanling Ji,Zhongli Cui,Yan Huang
β-1,3-Galactanases selectively degrade β-1,3-galactan, thus it is an attractive enzyme technique to map high-galactan structure and prepare galactooligosaccharides. In this work, a gene encoding exo -β-1,3-galactanase (PxGal43) was screened form Paenibacillus xylanexedens , consisting of a GH43 domain, a CBM32 domain and α-L-arabinofuranosidase B (AbfB) domain. Using β-1,3-galactan (AG-II-P) as substrate, the recombined enzyme expressed in Escherichia coli BL21 (DE3) exhibited an optimal activity at pH 7.0 and 30 °C. The enzyme was thermostable, retaining >70 % activity after incubating at 50 °C for 2 h. In addition, it showed high tolerance to various metal ions, denaturants and detergents. Substrate specificity indicated that PxGal43 hydrolysis only β-1,3-linked galactosyl oligosaccharides and polysaccharides, releasing galactose as an exo -acting manner. The function of the CBM32 and AbfB domain was revealed by their sequential deletion and suggested that their connection to the catalytic domain was crucial for the oligomerization, catalytic activity, substrate binding and thermal stability of PxGal43. The substrate docking and site-directed mutagenesis proposed that Glu191, Gln244, Asp138 and Glu81 served as the catalytic acid, catalytic base, pKa modulator, and substrate identifier in PxGal43, respectively. These results provide a better understanding and optimization of multi-domain bacterial GH43 β-1,3-galactanase for the degradation of arabinogalactan.
研究领域:
生物化学
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酶学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2019
·
IF 2.197
Xiaohui Wang,Qian Li,Junkang Sui,Jiamiao Zhang,Zhaoyang Liu,Jianfeng Du,Ruiping Xu,Yanyan Zhou,Xunli Liu
Soilborne pathogens affect plant growth and food production worldwide. The application of chemical fertilizers and pesticides to control plant diseases has harmful effects; fortunately, plant growth-promoting rhizobacteria can be used as a potential alternative strategy. Here, Paenibacillus jamilae HS-26 was selected for its highly antagonistic activity against several soilborne pathogens. The bacterium synthesized hydrolytic enzymes and released extracellular antifungal metabolites and volatile organic compounds—primarily, N, N-diethyl-1, 4-phenylenediamine, which was detected by gas chromatography-mass spectrometry and shown to inhibit fungal mycelial growth. Furthermore, HS-26 was useful for nitrogen fixation, phosphate and potassium solubilization, and siderophore and indoleacetic acid production. In vitro tests and pot experiments revealed that HS-26 considerably increased plant biometric parameters. Illumina MiSeq sequencing data showed a significant reduction in soilborne pathogens and increase in beneficial bacteria in the wheat rhizosphere after treatment with strain HS-26.
研究领域:
植物病理学
·
微生物学
·
农业科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Huajun Kang,Tengfei Fan,Zihan Lin,Yanxia Shi,Xuewen Xie,Lei Li,Sheng Xiang,Xiaowei Yuan,Xingsheng Li,Baoju Li,Ali Chai
Clubroot, caused by the obligate parasite Plasmodiophora brassicae , is one of the most important diseases of brassicas. The antagonistic bacterium Paenibacillus polymyxa ZF129 can suppress clubroot while its effectiveness is often unstable. To control clubroot more effectively, the macrobeads for controlled release of ZF129 were prepared using microencapsulation technology. Macrobeads with various ratios of chitosan (2 % w/w): carrageenan (0.3 % w/v) were prepared by an ionotropic gelation method and the bacteria ZF129 was loaded into macrobeads. The 1:1 chitosan: carrageenan showed the maximum swelling ratio (634 %), and the maximum survival rate (61.52 ± 1.12 %) after freeze-drying. Fourier transform infrared revealed the electrostatic interactions between chitosan and carrageenan. The macrobeads can efficiently release ZF129 strains into phosphate buffer solution and reach equilibrium in 48 h. The maximum number of bacteria cells to be released in the soil was observed after 25–30 days. The control efficacy of ZF129 macrobeads (chitosan: carrageenan, 1:1) and ZF129 culture against clubroot disease was 76.33 ± 3.65 % and 59.76 ± 4.43 % in greenhouse experiments, respectively and the control efficacy was calculated as 60.74 ± 5.00 % for ZF129 macrobeads and 40.94 ± 4.05 % for ZF129 culture under field experiments, respectively. The ZF129 macrobeads had significant growth-promoting effects on pak choi and Chinese cabbage. The encapsulation method described in this study is a prudent approach toward efficient biopesticides utilization with reduced environmental implications.
研究领域:
农业科学
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生物技术
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植物病理学
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微生物学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 190
·期 3
·826-838
·
IF 2.14
Liu Hang,Zhou Min,Ju Xin,Shu Hang,Hu Cuiying,Li Liangzhi
β-Glucosidase (BG) hydrolyzes cellobiose into glucose, and is a vital step in converting ionic liquids (ILs)-pretreated biomass to sustainable biofuels. The inactivation mechanism of BG from Paenibacillus sp. LLZ1 induced by microcrystalline cellulose was explored in various concentrations of ILs, composed of [Emim] + cation and [DEP] − , [OAc] − , [Br] − , [Cl] − , and [BF 4 ] − anions. The FTIR analysis of inactivated BG indicated that the ILs altered its β-sheet content. Moreover, circular dichroism spectroscopy (CD) suggested that the α-helix content decreased, while the β-sheet content increased with the presence of ILs in general. Interestingly, the secondary structure of BG had almost no change after [Emim]DEP treatment, while ionic liquid [Emim]BF 4 treatment caused the irreversible denaturation of BG. Eventually, by adding 0.4 mM of Aerosol OT surfactant, the BG activity was increased by 20.1% in the presence of 25% [Emim]DEP, and the corresponding glucose yield from hydrolysis of cellobiose was increased by 23.9%.
研究领域:
生物化学
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生物技术
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酶学
·
可持续能源
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
·
2026
·Vol. 76
·期 4
·007124
·
IF 2.3
Guo-Hong Liu,Ling Yao,Hui-Yuan Jiang,Yuan-Jia Xu,Peng Gao,Xie-Feng Lu,Christopher Rensing,Shun-Gui Zhou
Biological nitrogen fixation is central to sustainable rice cultivation, yet diazotrophicPaenibacillusspecies from red paddy soils remain poorly characterized. Here, three novelPaenibacillusstrains, sgz500958T, sgz500992Tand sgz5001063T, were isolated from red paddy soils in China. Phylogenetic and phylogenomic analyses based on 16S rRNA and whole-genome data revealed that the three strains formed distinct lineages withinPaenibacillus, with average nucleotide identity values below 95% relative to their closest reference strains. All strains possessed a completenifgene cluster and exhibited nitrogenase activity, and strain sgz5001063Tmaintained high activity at low concentrations (0–3 mM), was inhibited at intermediate concentrations (4–30 mM) and recovered at very high ammonium concentrations (30–300 mM). Utilizing complementary plant growth-promoting traits and co-culture compatibility, two nitrogen-fixing synthetic communities (NFSCs) were assembled. In rice assays, NFSC2 markedly enhanced germination (100%), shoot height (+72.5%) and root growth relative to sgz5001063Talone and NFSC1. The isolates are proposed asPaenibacillus oryziterraesp. nov. (sgz500958T=MCCC 1K09377T=KCTC 43740T),Paenibacillus paludissp. nov. (sgz500992T=MCCC 1K09376T=KCTC 43729T) andPaenibacillus diazotrophicussp. nov. (sgz5001063T=MCCC 1K09391T=KCTC 43741T).
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DOI
PubMed
高影响因子
International Journal of Peptide Research and Therapeutics
·
2017
·Vol. 25
·期 1
·161-168
·
IF 0.904
Yu Zhiliang,Zhang Ling,Qin Wangrong,Yin Jianhua,Qiu Juanping
Polymyxin E (colistin) has been considered as an important peptide antibiotic for clinical treatment of Gram-negative pathogens by selectively targeting lipopolysaccharide on the outer membrane. Recent reports showed that colistin can also kill Gram-positive bacteria, but the mechanism of colistin against Gram-positive bacteria remains unclear. In this study, we found that colistin can kill its producer Paenibacillus polymyxa , a Gram-positive bacterium, by both disrupting its cell integrity and inducing oxidative stress. Enhancement of the cell permeability is the major action of colistin against P. polymyxa . In relative, colistin-induced oxidative stress plays an associated role in killing of P. polymyxa . Notably, we, for the first time, found that exogenous catalase is able to intensify the colistin-induced killing by inhibiting the intracellular catalase activity and thus increasing oxidative stress. Our findings would shed light on a not yet clearly described bactericidal mechanism of colistin against Gram-positive bacteria. More importantly, our findings pave the road for the future to lower clinical use of colistin for avoiding bacterial resistance.
研究领域:
微生物学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 3
·387
·
IF 3.06
Zhiliang Yu,Yuyi Zhu,Jianv Fu,Juanping Qiu,Jianhua Yin
The commonly believed mechanism of colistin against Gram-negative bacteria is to cause cell membrane lysis, whereas the mechanism of colistin against Gram-positive bacteria is extremely fragmented. In this study, we found that colistin treatment onBacillus subtilisWB800,Paenibacillus polymyxaC12 andPaenibacillus polymyxaATCC842 enhances not only the activities of α-ketoglutaric dehydrogenase and malate dehydrogenase in tricarboxylic acid (TCA) cycle, but also the relative expression levels of their encoding genes. Additionally, the oxaloacetate concentration also increases. Interestingly, the analysis of the relative expression of genes specific for respiratory chain showed that colistin treatment stimulates the respiratory chain in Gram-positive bacteria. Accordingly, the NAD+/NADH ratio increases and the oxidative level is then boosted up. As a result, the intensive oxidative damages are induced in Gram-positive bacteria and cells are killed. Notably, both rotenone and oligomycin, respectively, inhibiting NADH dehydrogenase and phosphorylation on respiratory chain can downgrade oxidative stress formation, thus alleviating the colistin-induced killing of Gram-positive cells. Besides, thiourea-based scavenging for reactive oxygen species also rescues the colistin-subjected cells. These data collectively demonstrate that colistin stimulates both TCA cycle and respiratory chain in Gram-positive bacteria, leading to the enhancement of NADH metabolism and resulting in the generation of oxidative damages in Gram-positive cells. Our studies provide a better understanding of antibacterial mechanism of colistin against Gram-positive bacteria, which is important for knowledge on bacterial resistance to colistin happening via the inhibition of respiratory chain and manipulation of its production.Keywords:colistin;Gram-positive bacteria;oxidative stress;TCA cycle;respiratory chain
研究领域:
微生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Xu Xiaodong,Ding Zhao,Pu Chunlin,Kong Changchang,Chen Shijunyin,Lu Weiling,Zhang Jianfa
Introduction: Overexposure to ultraviolet (UV) light is known to cause damage to the skin, leading to sunburn and photo-aging. Chemical sunscreen products may give rise to health risks including phototoxicity, photosensitivity, and photosensitivity. Natural polysaccharides have attracted considerable interests due to diverse biological activities.Methods: A novel polysaccharide isolated was purified and structurally characterized using chemical methods followed by HPLC, GLC-MS, as well as 1D and 2D NMR spectroscopy. The photoprotective effect of the EPS on UVB-induced damage was assessed in vitro using cultured keratinocytes and in vivo using C57BL/6 mouse models.Results: The average molecular weight of the EPS was 5.48 × 106 Da, composed of glucose, mannose and galactose residues at a ratio of 2:2:1. The repeating units of the EPS were →3)-β-D-Glcp (1→3) [β-D-Galp (1→2)-α-D-Glcp (1→2)]-α-D-Manp (1→3)-α-D-Manp (1→. In cultured keratinocytes, the EPS reduced cytotoxicity and excessive ROS production induced by UVB irradiation. The EPS also exhibits an inhibitory effect on oxidative stress, inflammation, and collagen degradation found in the photodamage in mice. 1H NMR-based metabolomics analysis for skin suggested that the EPS partly reversed the shifts of metabolic profiles of the skin in UVB-exposed mice.Conclusion: The EPS exhibits skin photoprotective effects through regulating oxidative stress both in vivo and in vitro. Our findings highlight that the EPS is a potential candidate in sunscreen formulations for an efficient solution to UVB radiation.
研究领域:
生物化学
·
分子生物学
·
皮肤科学
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产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2025
·
IF 3.8
Fan Yang,Wei Dai,Hua Xue,Wen Chen,Chen Liu,Yaru Tian,Wanli Cheng,Jibin Zhang
Root-knot nematodes (RKNs) are notoriously challenging to control because of their broad host spectrum and swift proliferation. Consequently, there is an urgent need to develop safe and effective nematicides. Microorganisms and their metabolites have the potential to control RKNs safely and effectively. Paenibacillus polymyxa KM2501-1 can produce a range of volatile organic compounds (VOCs) with nematicidal activities, among which 2-undecanol is a potential biocontrol agent against Meloidogyne incognita. Nonetheless, the mechanism by which 2-undecanol prevents RKN infection in plant roots remains unclear. 2-Undecanol has many activities against M. incognita in vitro, including contact (LC50 = 34.5 mg/L), fumigation (LC50 = 191.6 mg/L), attraction, locomotion inhibition (frequency of head thrash and body bend reduced by 84.0% and 97.0% at concentration of 40 mg/L) and egg hatching inhibition (by 98.5% at concentration of 80 mg/L). Upon treatment with 2-undecanol, a noticeable reduction in the number of galls was observed, and the control efficacy reached 60.8% at the dose of 5 mg/pot. Metabolomics analysis revealed that the application of 2-undecanol significantly influenced the composition of root exudates of tomato. Among the 17 differential metabolites, the up-regulated 10-undecenal could attract and kill M. incognita J2 larvae at a concentration of 100 mg/L, and cyclohexylamine had nematicidal activity at the concentration of 1,000 mg/L. This study revealed the mechanism of 2-undecanol inducing plant roots to secrete nematicidal substances with attractant activity, effectively controlling RKNs, which could pave the way for the development of environmentally friendly and cost-effective nematicides.
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产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2023
·Vol. 11
·期 3
·
IF 3.7
Kun Wang,Ziyan Lin,Jin Dou,Mingguo Jiang,Naikun Shen,Jing Feng
The role of microbial volatile organic compounds (MVOCs) in promoting plant growth has received much attention. We isolated Paenibacillus peoriae from mangrove rhizosphere soil, which can produce VOCs to promote the growth of Arabidopsis thaliana seedlings, increase the aboveground biomass of A. thaliana, and increase the number of lateral roots of A. thaliana. The effects of different inoculation amounts and different media on the composition of MVOCs were studied by solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) and headspace sampler/GC-MS. We found that the growth medium influences the function and composition of MVOCs. To survey the growth-promoting functions, the transcriptome of the receptor A. thaliana was then determined. We also verified the inhibitory effect of the soluble compounds produced by P. peoriae on the growth of 10 pathogenic fungi. The ability of P. peoriae to produce volatile and soluble compounds to promote plant growth and disease resistance has shown great potential for application in the sustainability of agricultural production.IMPORTANCE Microbial volatile organic compounds (MVOCs) have great potential as “gas fertilizers” for agricultural applications, and it is a promising research direction for the utilization of microbial resources. This study is part of the field of interactions between microorganisms and plants. To study the function and application of microorganisms from the perspective of VOCs is helpful to break the bottleneck of traditional microbial application. At present, the study of MVOCs is lacking; there is a lack of functional strains, especially with plant-protective functions and nonpathogenic application value. The significance of this study is that it provides Paenibacillus peoriae, which produces VOCs with plant growth-promoting effects and broad-spectrum antifungal activity against plant-pathogenic fungi. Our study provides a more comprehensive, new VOC component analysis method and explains how MVOCs promote plant growth through transcriptome analysis. This will greatly increase our understanding of MVOC applications as a model for other MVOC research.
研究领域:
微生物学
·
植物科学
·
农业可持续性
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产品关联
DOI
PubMed
高影响因子
SOIL BIOLOGY & BIOCHEMISTRY
·
2023
·
IF 9.7
Jipeng Wang,Qian Zhao,Yiqiu Zhong,Shuhao Ji,Guanrui Chen,Qingqing He,Yanhong Wu,Haijian Bing
Free-living nitrogen fixation (FLNF) is a vital source of nitrogen for the initiation and development of ecosystems on bare lands. This process is catalyzed by the enzyme nitrogenase and is energy intensive. Of the three nitrogenase isoforms, molybdenum nitrogenase (Mo-Nase) is more efficient than its vanadium (V) and iron (Fe) counterparts at room temperature. However, the acquisition of Mo, one of the scarcest biometals in soils, represents a major resource investment for soil diazotrophs. In a vegetation restoration chronosequence developed on barren (low carbon and nitrogen) soils of a high-V region, we tested the following two hypotheses. First, the FLNF rate would be limited by the supply of energy. Second, high V availability would cut the cost of V acquisition, and it would be beneficial for diazotrophs to use V-Nase as a complement of Mo-Nase. For the soils collected along the chronosequence, the addition of a carbon cocktail remarkably stimulated the FLNF rate by relieving the energy constraint and by enriching the diazotrophic genus Paenibacillus . Despite the high V content in the soils, we failed to detect the vnf genes of V-Nase by metagenomic sequencing. Meanwhile, we observed a limited contribution of V-Nase activity to FLNF by checking the R ratio (ratio of FLNF rates measured by acetylene reduction and 15 N 2 incorporation) and the production of ethane during acetylene reduction. The reason might be that the Mo availability and temperature in our study area did not reach the thresholds for the onset of V-Nase activity. Overall, our results highlight the importance of carbon supply for FLNF in barren soils, indicating that plants may effectively regulate rhizosphere diazotrophs by secreting root exudates. This may be an important mechanism by which nonnitrogen-fixing plants survive on nitrogen-poor soils at the beginning of primary succession.
研究领域:
生态学
·
微生物学
·
土壤学
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DOI
高影响因子
危险品化学品经营许可证（带存储）