ACS Applied Nano Materials
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2019
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IF 0
Shuo Li,Jiawei Liu,Guilin Zhu,Heyou Han
Conformal deposition of Pt-dots arrays on polyhedral Pd nanocrystals can enhance Pt catalytic properties and reduce its loading. Herein, a simple method for the fabrication of Pd@Pt core–shell nanodot arrays (Pd@PtNAs) heterostructures via reducing H2PtCl6 and Na2PdCl4 by l-ascorbic acid in an aqueous solution with cetylpyridinium chloride and KBr is reported. The Pt nanodots arrays with a small size (about 3 nm in diameter) were dispersed on the surface of Pd decahedra. Such ultrasmall size Pt dots arrays can effectively increase the proportion of low-coordination Pt atoms. Compared with commercially available Pt/C catalysts, the Pd@PtNAs show obvious improvement in catalytic performance applied to oxygen reduction reaction. The mass activity (0.20 A/mgPt) of the obtained Pd@PtNAs with 36.9 wt % Pt shows 2.2 times relative to Pt/C catalyst (0.09 A/mgPt) for ORR. What’s more, the Pd@PtNAs with 36.9 wt % Pt exhibited highly stable property and mass activity with only 11% loss after 10 000 testing cycle.
研究领域:
材料科学
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电化学
·
催化
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产品关联
DOI
Theranostics
·
2020
·
IF 8.579
Yu Zhang,Quanyi Liu,Chong-Bo Ma,Qingqing Wang,Meiting Yang,Yan Du
Alcohol abuse causes health problems and security accidents. A reliable and sensitive detection system for alcohol has been an instinctive demand in law enforcement and forensic. More efforts are demanded in developing new sensing strategy preferably with portable and non-invasive traits for the pushforward of point-of-care (POC) device popularization. Methods : We developed a POC diagnosis system for alcohol assay with the aid of alcohol oxidase (AOX) pre-joining in the system as well as Pd@Pt core-shell nanoparticles (abbreviated to Pd@Pt) that were decorated on ploy(vinyl alcohol) aerogel with amphiphilicity. Biological samples like saliva and whole blood can be absorbed by the aerogel in a quick process, in which the analyte would go through a transformation from alcohol, H 2 O 2 , to a final production of O 2 , causing an analyte dose-dependent signal change in the commercial portable pressure meter. The cascade reactions are readily catalyzed by AOX and Pd@Pt, of which the latter one possesses excellent peroxidase-like activity. Results : Our design has smartness embodied in the aerogel circumvents the interference from methanol which is more ready to be catalyzed by AOX. Under the optimal conditions, the limit of detection for alcohol was 0.50 mM in saliva, and is able to distinguish the driving under the influence (DUI) (1.74 mM in saliva) and driving while impaired (DWI) (6.95 mM in saliva) in the national standard of China. Conclusion : Our proof-of-concept study provides the possibility for the establishment of POC device for alcohol and other target detection, not only owing to the sensing qualification but also thanks to the architecture of such sensor that has great flexibility by replacing the AOX with glucose oxidase (GOX), thenceforth realizing the accurate detection of glucose in 0.5% whole blood sample. With the advantages of easy accessibility and anti-interference ability, our sensor exhibits great potential for quantitative diagnostics in biological system.
研究领域:
生物传感
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法医学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ChemNanoMat
·
2023
·Vol. 9
·期 5
·e202200569
·
IF 3.8
Yanyu Li,Xiaoyue Zhang,Jinglin Shen,Wei Qi
Graphical A new type of nanocomposite enzymes has been developed, composed of Pd−Pt nanoparticles, hemin-rGO and CNTs-COOH. The nanocomposites show a high peroxidase-like activity and a linear response toward H 2 O 2 in the range of 0.05–500 μM with a determination limit of 0.01 μM. Based on this, a paper sensor was constructed for H 2 O 2 detection in real samples. Nanocomposite enzymes are attracting intensive attention worldwide. In the present study, a novel colorimetric H 2 O 2 paper sensor was developed based on a new type of nanocomposites composed of Pd−Pt nanoparticles, hemin functionalized reduced graphene oxide (hemin-rGO) and carboxylated carbon nanotubes (CNTs-COOH). The proposed nanocomposites show a high peroxidase-like activity. It provides a sensitive platform showing a linear response toward H 2 O 2 in the range of 0.05–500 μM with a determination limit of 0.01 μM. Based on this, the composites were applied to construct a paper sensor for H 2 O 2 detection in real samples. The cost-effectiveness, simplicity and efficiency of this paper sensor could be developed for “point-of-care” analysis and other related areas.
研究领域:
生物化学
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2022
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IF 6.556
Xin Wang,Min Zhang,Xiaoxu Pang,Kunlun Huang,Zhiyi Yao,Xiaohong Mei,Nan Cheng
Staphylococcus aureus is a frequent pathogenic bacterium that has a significant detrimental influence on the health of the human body. Therefore, developing a practical and portable detection platform is critical to ensuring food safety. Nanozymes are a kind of engineered nanomaterials with superior enzyme-like activities, providing infinite possibilities for the development of highly sensitive analytical assays. In this study, mesoporous core−shell [email protected] ( [email protected] ) nanozymes were synthesized and then applied as a signal amplifier in Staphylococcus aureus colorimetric immunoassay . At the same time, a careful comparative study of the catalytic performance with natural horseradish peroxidase (HRP), [email protected] nanozymes and their complexes [email protected] (HRP coupling with [email protected] nanozymes) were firstly performed, as well as clever using a colorimeter to achieve portable signal output. [email protected] bioprobes enable remarkable peroxidase-like catalytic activity, resulting in the highest sensitivity with a limit of detection (LOD) improved from 1 × 10 5 to 1 × 10 3 CFU/mL. In all, this work conducted a valuable comparative study and presented an improved strategy for the portable detection of pathogenic bacteria, which was expected to be used in a wide range of applications in food safety regulation and biomedicals arenas.
研究领域:
分析化学
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生物技术
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食品安全
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生物传感器
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Mingdang Li,Ping Wang,Faying Li,Qingyan Chu,Yueyun Li,Yunhui Dong
Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative monitoring of prostate specific antigen (PSA). The sulfo group functionalized multi-walled carbon nanotubes (MWCNTs-SO 3 H) were used as substrate material to increase the specific surface area and enhance the conductivity of the glassy carbon electrode. Gold nanoparticles (Au NPs) were introduced to enhance the load capacity of the substrate material for primary antibodies (Ab 1 ) and accelerate the electron transfer on the electrode interface. The mesoporous core-shell Pd@Pt nanoparticle loaded by amino group functionalized graphene (M-Pd@Pt/NH 2 -GS) with high specific surface area, high indexed facets, and good biocompatibility was not only as the carriers of secondary antibodies (Ab 2 ) but also catalyzed the reduction of hydrogen peroxide (H 2 O 2 ), which effectually amplified the current signal in detection of PSA. The as-proposed immunosensor exhibited high sensitivity and stability on the detection of PSA. A linear relationship between current signals and the concentrations of PSA was obtained in the range from 10 fg/mL to 50 ng/mL and the detection limit of PSA was 3.3 fg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of PSA.
研究领域:
生物医学工程
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 11
·期 4
·443-453
·
IF 2.073
Pramod K. Kalambate,Yankai Li,Yue Shen,Yunhui Huang
In this work, a novel highly sensitive electrochemical sensing platform has been developed for simultaneous determination of anticancer drugs doxorubicin (DOX) and dasatinib (DAS) by means of mesoporous Pd@Pt core–shell nanoparticles supported on MWCNT (Pd@Pt/MWCNT). The electrochemical behaviour of the drugs was assessed through cyclic voltammetry, while simultaneous determination was carried out using adsorptive stripping square wave voltammetry (AdSSWV). Under optimized conditions, the method exhibits linear responses towards simultaneous determination of DOX and DAS in the concentration ranges of 4.4–8580 nM and 38–9880 nM, with detection limits of 0.86 and 6.72 nM, respectively. Furthermore, the proposed platform was successfully used for reliable determination of DOX and DAS in urine and blood serum samples with good accuracy and precision. The satisfactory recoveries obtained in blood serum and urine samples showed the potential for application of the proposed platform in quality control in the pharmaceutical industry, clinical analysis and optimization of chemotherapeutic treatment. We found that the established sensor has several advantages in terms of high sensitivity and selectivity, ease of preparation, short analysis time, long-term stability and fast response.
研究领域:
分析化学
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药物分析
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癌症治疗
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 48
·5091-5099
·
IF 3.532
Yue Sun,Zihao Xie,Fubin Pei,Wei Hu,Shasha Feng,Qingli Hao,Bing Liu,Xihui Mu,Wu Lei,Zhaoyang Tong
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seriously threatened global public health. Establishing a rapid and sensitive diagnostic test for early detection of the SARS-CoV-2 nucleocapsid protein is urgently required to defend against the pandemic. Herein, an enhanced lateral flow immunoassay (LFIA) was fabricated by trimetallic Au@Pd@Pt core–shell nanozymes for detection of the SARS-CoV-2 nucleocapsid protein. The Au@Pd@Pt nanozymes (Au@Pd@Pt NZs) synthesized via a one-pot method, with a dendrite morphology and uniform particle size, showed excellent peroxidase-like activity. Due to the perfect enzyme-like catalytic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), the catalytic signal could be generated even by a tiny amount of Au@Pd@Pt NZs accumulated on the test strip. Therefore, rapid detection with higher sensitivity was achieved. The Au@Pd@Pt NZs-based LFIA provided a quantitative range of 0.05–100 ng mL−1 with a limit of detection of 0.037 ng mL−1, which was 17-fold lower than the LFIA without enhancement. The average recoveries from spiked samples were in the range of 92.5–107.9% with relative standard deviations all less than 4%, indicating the reliability and repeatability of the proposed LFIA. Additionally, the proposed LFIA could report results within 30 min using a microplate reader. In conclusion, the Au@Pd@Pt NZs-LFIA is a rapid, simple, and sensitive method for detecting the SARS-CoV-2 nucleocapsid protein.
研究领域:
分析化学
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纳米技术
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病毒学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2017
·
IF 3.012
Mingdang Li,Ping Wang,Fubin Pei,Haoxuan Yu,Peng Chen,Yunhui Dong,Yueyun Li,Qing Liu,Degang Li
In this work, a highly sensitive sandwich-type electrochemical immunosensor was fabricated by using the gold nanorods (Au NRs) as the substrate material and the mesoporous Au@Pd@Pt core-shell nanospheres (m-Au@Pd@Pt) as the label of the detection antibodies (Ab 2 , Ab 2 label) for detecting Hepatitis B surface antigen (HBs Ag). The Au NRs with excellent conductivity and biocompatibility can accelerate the electron transfer on the electrode interface and enhance the load capacity of capture antibodies (Ab 1 ). The m-Au@Pd@Pt with mesoporous structure can not only provide high specific surface area and abundant catalytically active sites, but also offer great pore accessibility of guest species from outside. The detection signal of the immunosensor was effectively improved by the novel Ab 2 label. With the good cooperation between Au NRs and Ab 2 label, a linear relationship between current signal and the logarithm of the HBs Ag concentration was obtained in the wide range of 20 fg/mL to 200 ng/mL and the detection limit of HBs Ag was 6.7 fg/mL (signal-to-noise ratio of 3). Besides, the designed immunosensor shows good reproducibility, long-term stability and high specificity. Crucially, the sensitive and selective response to HBs Ag in real human serum samples confirms that the designed immunosensor will be promising in the clinical diagnosis of biomarkers.
研究领域:
纳米技术
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生物传感器
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分析化学
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医学诊断
✓ 已验证
DOI
高影响因子
BIOINORGANIC CHEMISTRY AND APPLICATIONS
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2023
·
IF 3.8
Jingfang Song,Jian He,Lin Yang,Weiguo Wang,Qinqin Bai,Wei Feng,Ranhui Li
To inhibit the growth of bacteria, the DA-PPI nanozyme with enhanced peroxidase-like activity was synthesized. The DA-PPI nanozyme was obtained by depositing high-affinity element iridium (Ir) on the surface of Pd-Pt dendritic structures. The morphology and composition of DA-PPI nanozyme were characterized using SEM, TEM, and XPS. The kinetic results showed that the DA-PPI nanozyme possessed a higher peroxidase-like activity than that of Pd-Pt dendritic structures. The PL, ESR, and DFT were employed to explain the high peroxidase activity. As a proof of concept, the DA-PPI nanozyme could effectively inhibit E. coli (G−) and S. aureus (G+) due to its high peroxidase-like activity. The study provides a new idea for the design of high active nanozymes and their application in the field of antibacterial.
研究领域:
生物化学
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材料科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Science Bulletin
·
2025
·
IF 21.1
Qing Zeng,Mengyuan Ma,Hui Liu,Lin Xu,Shaonan Tian,Dong Chen,Jing Wang,Jun Yang
Atomic surface engineering for nanostructures significantly contributes to the enhancement of electrocatalysis for a given chemical reaction. However, exploring a facile method to elaborately regulate surfaces at atomic scale remains a grand challenge. Herein, we report the construction of atomically rough surfaces (ARSs) on Au-based binary alloys through a novel and controllable gold (Au)-catalyzed strategy, which involves the first synthesis of Au-based bimetallic nanoalloys, i.e., AuPd and AuAg, and subsequent reduction of another metal ions (Pt, Pd, or Ag) initiated by Au sites on the alloy particle surfaces. By combining ARSs with low-coordinated atoms with ligand effect induced by vicinal Au atoms, the as-prepared ARSs exhibit good activity and durability toward ethanol oxidation reaction (EOR) in an alkaline medium. In particular, the Pd-Pt ARSs on the AuPd alloy particle surface (denoted as AuPd-Pt) exhibit the highest electrocatalytic EOR performance in terms of both specific activity (14.9 mA cm –2 ) and mass activity (28.5 A mg –1 ), surpassing those of their AuPd alloy counterparts, commercial Pd/C catalyst, and most Pd-based electrocatalysts reported recently. In situ Fourier transform infrared (FTIR) spectroscopy reveals that the EOR process on the Pd-Pt ARSs strongly prefers incomplete oxidation, which is further authenticated by the density functional theory (DFT) calculations.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Qianqian Cao,Yiqin Liu,Lin Yang,Ting Tan,Jian He,Weiwei Chen,Ranhui Li,Weiguo Wang
Background The lateral flow immunoassay (LFIA) is widely employed as a point-of-care testing (POCT) technique. However, its limited sensitivity hinders its application in detecting biomarkers with low abundance. Recently, the utilization of nanozymes has been implemented to enhance the sensitivity of LFIA by catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The catalytic performance of nanozymes plays a crucial role in influencing the sensitivity of LFIA. Results The Cornus officinalis Sieb. et Zucc-Pd@Pt (CO–Pd@Pt) nanozyme with good peroxidase-like activity was synthesized herein through a facile one-pot method employing Cornus officinalis Sieb. et Zucc extract as a reducing agent. The morphology and composition of the CO–Pd@Pt nanozyme were characterized using TEM , SEM , XRD , and XPS . As a proof of concept, the as-synthesized CO–Pd@Pt nanozyme was utilized in LFIA (CO–Pd@Pt-LFIA) for the detection of human chorionic gonadotropin (hCG). Compared to conventional gold nanoparticles-based LFIA (AuNPs-LFIA), CO–Pd@Pt-LFIA demonstrated a significant enhancement in the limit of detection (LOD, 0.08 mIU/mL), which is approximately 160 times lower than that of AuNPs-LFIA. Furthermore, experiments evaluating accuracy, precision, selectivity , interference, and stability have confirmed the practical applicability of CO–Pd@Pt-LFIA for hCG content determination. Significance The present study presents a novel approach for the synthesis of bimetallic nanozymes through environmentally friendly methods, utilizing plant extracts as both protective and reducing agents. Additionally, an easily implementable technique is proposed to enhance signal detection in lateral flow immunoassays.
研究领域:
生物化学
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纳米医学
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诊断学
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Shi Fenglei,Tieu Peter,Hu Hao,Peng Jiaheng,Zhang Wencong,Li Fan,Tao Peng,Song Chengyi,Shang Wen,Deng Tao,Gao Wenpei,Pan Xiaoqing,Wu Jianbo
Corrosion of electrocatalysts during electrochemical operations, such as low potential - high potential cyclic swapping, can cause significant performance degradation. However, the electrochemical corrosion dynamics, including structural changes, especially site and composition specific ones, and their correlation with electrochemical processes are hidden due to the insufficient spatial-temporal resolution characterization methods. Using electrochemical liquid cell transmission electron microscopy, we visualize the electrochemical corrosion of Pd@Pt core-shell octahedral nanoparticles towards a Pt nanoframe. The potential-dependent surface reconstruction during multiple continuous in-situ cyclic voltammetry with clear redox peaks is captured, revealing an etching and deposition process of Pd that results in internal Pd atoms being relocated to external surface, followed by subsequent preferential corrosion of Pt (111) terraces rather than the edges or corners, simultaneously capturing the structure evolution also allows to attribute the site-specific Pt and Pd atomic dynamics to individual oxidation and reduction events. This work provides profound insights into the surface reconstruction of nanoparticles during complex electrochemical processes.
研究领域:
材料科学
·
电化学
·
纳米技术
·
催化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）