JOURNAL OF FOOD BIOCHEMISTRY
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2020
·Vol. 44
·期 12
·e13530
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IF 1.662
Zhen-Xing Wang,Qing-Qing Lin,Zong-Cai Tu,Lu Zhang
In this study, the influence of in vitro gastrointestinal digestion on the Perilla frutescens leaf extract (PFLE) were measured. Results revealed that total phenolic content (TPC) and total flavonoid content (TFC) were significantly decreased after simulated digestion (ca. 53% of phenolics and 40% of flavonoids). The IC 50 value of DPPH· scavenging activity and ABTS + scavenging ability increased by 23% and 56%, respectively, while ferric reducing antioxidant power reduced by 53%. For the inhibition ability on α-glucosidase, acetylcholinesterase, and MCF-7 cell proliferation, their IC 50 values increased by 360%, 197%, and 25%, respectively. Three phenolic acids and one flavonoid in PFLE were quantified by high-performance liquid chromatography. Overall, although significant losses of the active components and biological activities occurred during in vitro gastrointestinal digestion, it still showed the potential as an oral agent for treatment and prevention of oxidative stress, cancer, diabetes, and Alzheimer's disease. Practical applications As an important annual herbaceous plant with rich biochemical compounds and many biological functions, Perilla frutescens leave is widely used in the food and traditional Chinese medicine. However, the dynamic changes of its active compounds and activities during the digestion process are unclear. In this study, the digestion results in significant loss of the active ingredients and biological activities of P. frutescens leaf extract (PFLE), particularly in the gastric digestion. In addition, PFLE remains to show certain antioxidant activity, α-glucosidase inhibitory ability, acetylcholinesterase inhibitory ability, and MCF-7 cell proliferation inhibitory ability after digestion. Therefore, this research might facilitate further research and development of P. frutescens .
研究领域:
药理学
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营养学
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食品科学
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药物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 6
·3006-3016
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IF 4.125
Xiaoxiang Han,Jianghui Huang,Jinwang Cai,Chen Yu,Shengchou Jiang,Qing Ye,Shuifen Yang,Weichun Pan,Qiaozhi Zhang,Yanbo Wang
Background As potent antioxidants, anthocyanins can protect the body from free radicals. However, the traditional solvent extraction method has the disadvantages of requiring a high extraction temperature and long extraction time, so it is necessary to develop an efficient extraction method for anthocyanins. Results In this study, the technique of natural deep eutectic solvents (DESs) was applied to extract anthocyanins from purple perilla leaves with the aid of microwave-ultrasonic assisted extraction (MUAE). The response surface methodology (RSM), based on the Box–Behnken design (BBD), predicted the maximum extraction yield of anthocyanins to be 619.62 mg (100 g) −1 under the following conditions: x 1 (ultrasonic extraction power) = 357.25 W, x 2 (time) = 25.62 min, and x 3 (temperature) = 57.80 °C. The biological activity of the extract obtained was evaluated by examining its radical-scavenging effect on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radical, and superoxide anion radicals. Its bacteriostatic impact was investigated on four typical bacteria: Shewanella putrefaciens ( S. putrefaciens ), Pseudomonas fluorescens ( P. fluorescens ), Escherichia coli ( E. coli ), and Staphylococcus aureus ( S. aureus ). Conclusion The integrated extraction method of DESs with MUAE was efficient, energy-saving, green, and sustainable. © 2022 Society of Chemical Industry.
研究领域:
食品科学
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化学工程
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植物化学
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生物技术
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 22
·7554
·
IF 4.6
Pengfei Jiao,Xingmei He,Shihua Ma,Shengping Wang,Qiuhong Niu
The development of natural antioxidants to replace synthetic compounds is attractive.Perilla frutescensleaves were proven to be rich in antioxidants. The extraction of antioxidants from Perilla leaves via ultrasonic-assisted extraction (UAE) based on choline chloride-based deep eutectic solvents (DESs) was studied. Firstly, several DESs were prepared, and their extraction effects were compared. Secondly, the extraction process was optimized by single-factor experiments and response surface methodology (RSM). Finally, the optimization results were verified and compared with the results of traditional solvent-based UAE. The effects of solvents on the surface cell morphology ofPerilla frutescensleaves were characterized by scanning electron microscopy (SEM). Choline chloride-acetic acid-based DES (ChCl-AcA) extract showed a relatively high ferric-reducing antioxidant activity (FRAP) and 2,2-diphenyl-1-picrylhyldrazyl radical scavenging rate (DPPH). Under the optimal operating conditions (temperature 41 °C, liquid–solid ratio 33:1, ultrasonic time 30 min, water content 25%, ultrasonic power 219 W), the experimental results are as follows: DPPH64.40% and FRAP0.40 mM Fe(II)SE/g DW. The experimental and predicted results were highly consistent with a low error (<3.38%). The values of the DPPH and FRAP were significantly higher than that for the water, ethanol, and butanol-based UAE. SEM analysis confirmed that ChCl-AcA enhanced the destruction of the cell wall, so that more antioxidants were released. This study provides an eco-friendly technology for the efficient extraction of antioxidants fromPerilla frutescensleaves. The cytotoxicity and biodegradability of the extract will be further verified in a future work.Keywords:extraction;deep eutectic solvent;natural antioxidant;response surface optimization;Perilla leaf
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DOI
PubMed
高影响因子
RESEARCH IN VETERINARY SCIENCE
·
2026
·
IF 2.1
Jia-Yu Ye,Wei Zhang,Mei Qin,Hong-Jie Cui,Jian Li
Background The gut-liver axis is integral to the pathophysiology of chronic liver inflammation and related metabolic inflammatory disease. Although Perilla frutescens seed oil (PFSO) possesses anti-inflammatory properties, its clinical application is limited by its poor stability and bioavailability. This study aimed to develop a PFSO-loaded nanoemulsion (PFSO-NE) to enhance its therapeutic efficacy. Methods The PFSO-NE was optimized using pseudoternary phase diagrams, and its physicochemical properties were thoroughly characterized. The therapeutic effects of PFSO-NE were assessed in lipopolysaccharide (LPS) mice using a combination of biochemical assays, histological examinations, quantitative PCR, 16S rDNA sequencing, and short-chain fatty acid (SCFA) analyses. Results PFSO-NE demonstrated a small particle size (32.77 nm), high encapsulation efficiency (93.02%), and enhanced stability. This led to a significant reduction in the levels of inflammatory cytokines, specifically TNF-α and IL-6, increased IL-10 levels, mitigated tissue damage, and modulated the gut microbiota by enriching beneficial bacteria, such as Lachnospiraceae, and elevating acetate concentrations. Conclusions PFSO-NE effectively attenuates inflammation in the gut-liver axis by enhancing bioavailability, modulating immune responses, and remodeling the gut microbiota, thereby providing preclinical evidence for PFSO-NE as a promising natural nanoformulation for the potential treatment of inflammatory diseases.
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DOI
PubMed
高影响因子
JOURNAL OF PLANT PHYSIOLOGY
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2023
·
IF 4.3
Qinyong Dong,Dong Li,Yangliu Wu,Chunran Zhou,Yongxi Lin,Peijuan Miao,Jiaqi Li,Canping Pan
Few studies have been conducted to investigate the impact of pesticides on the secondary metabolism of traditional Chinese medicine and strategies to mitigate the toxicity of pesticide-induced oxidative stress. The current study focuses on evaluating the potential impacts of nano selenium (NSe) and imidacloprid (IMI) on the quality, physiological biochemistry, and secondary metabolites in Perilla frutescens (L.) Britt . ( P. frutescens ). The study utilized metabolome analysis to explore the toxicity mechanism of IMI. The study noted that IMI-induced stress could emerge with detrimental effects by targeting the destruction of the phenylpropanoid biosynthesis pathway. IMI-induced phenylpropanoid metabolism disorder resulted in an 8%, 17%, 25%, 10%, 65%, and 29% reduction in phenylalanine, coniferyl aldehyde, ferulic acid, cafestol, p-coumaraldehyde, and p-coumaric acid levels, respectively. Under the treatment of exogenous NSe, the levels of these metabolites were increased by 16%, 32%, 22%, 22%, 92%, and 29%, respectively. The application of exogenous NSe increased the levels of these metabolites and improved the biochemical disorder and quality of P. frutescens leaves by optimizing the phenylpropanoid metabolic pathway and enhancing the antioxidant system. Overall, the results suggest that foliar application of NSe could alleviate the oxidative stress toxicity induced by IMI and improve the quality of P. frutescens .
研究领域:
植物生理学
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药理学
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农业科学
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DOI
PubMed
高影响因子
Frontiers in Nutrition
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2022
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IF 6.59
Wang Zhenxing,Jin Ximeng,Zhang Xuechun,Xie Xing,Tu Zongcai,He Xiahong
This study aimed to investigate the impact of probiotic fermentation on the active components and functions of Perilla frutescens leaves (PFL). PFL was fermented for 7 days using six probiotics (Lactobacillus Plantarum SWFU D16, Lactobacillus Plantarum ATCC 8014, Lactobacillus Rhamnosus ATCC 53013, Streptococcus Thermophilus CICC 6038, Lactobacillus Casei ATCC 334, and Lactobacillus Bulgaricus CICC 6045). The total phenol and flavonoid contents, antioxidant abilities, as well as α-glucosidase and acetylcholinesterase inhibition abilities of PFL during the fermentation process were evaluated, and its bioactive compounds were further quantified by high-performance liquid chromatography (HPLC). Finally, non-targeted ultra-HPLC–tandem mass spectroscopy was used to identify the metabolites affected by fermentation and explore the possible mechanisms of the action of fermentation. The results showed that most of the active component contents and functional activities of PFL exhibited that it first increased and then decreased, and different probiotics had clearly distinguishable effects from each other, of which fermentation with ATCC 53013 for 1 day showed the highest enhancement effect. The same trend was also confirmed by the result of the changes in the contents of 12 phenolic acids and flavonoids by HPLC analysis. Further metabolomic analysis revealed significant metabolite changes under the best fermentation condition, which involved primarily the generation of fatty acids and their conjugates, flavonoids. A total of 574 and 387 metabolites were identified in positive ion and negative ion modes, respectively. Results of Spearman’s analysis indicated that some primary metabolites and secondary metabolites such as flavonoids, phenols, and fatty acids might play an important role in the functional activity of PFL. Differential metabolites were subjected to the KEGG database and 97 metabolites pathways were obtained, of which biosyntheses of unsaturated fatty acids, flavonoid, and isoflavonoid were the most enriched pathways. The above results revealed the potential reason for the differences in metabolic and functional levels of PFL after fermentation. This study could provide a scientific basis for the further study of PFL, as well as novel insights into the action mechanism of probiotic fermentation on the chemical composition and biological activity of food/drug.
研究领域:
食品科学
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药理学
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代谢组学
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生物化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Chinese Medicine
·
2023
·Vol. 18
·期 1
·1-20
·
IF 4.9
Xu Yang-ding,Guo Yu-jie,Mao He-rong,Xiong Zhi-xiang,Luo Meng-yu,Luo Rui-qi,Lu Shan,Huang Lu,Hong Yi
Background Dermatophyte caused by Trichophyton mentagrophytes is a global disease with a growing prevalence that is difficult to cure. Perilla frutescens (L.) Britt. is an edible and medicinal plant. Ancient books of Traditional Chinese Medicine and modern pharmacological studies have shown that it has potential anti-fungi activity. This is the first study to explore the inhibitory effects of compounds from P. frutescens on Trichophyton mentagrophytes and its mechanism of action coupled with the antifungal activity in vitro from network pharmacology, transcriptomics and proteomics.Methods Five most potential inhibitory compounds against fungi in P. frutescens was screened with network pharmacology. The antifungal activity of the candidates was detected by a broth microdilution method. Through in vitro antifungal assays screening the compound with efficacy, transcriptomics and proteomics were performed to investigate the pharmacological mechanisms of the effective compound against Trichophyton mentagrophytes. Furthermore, the real-time polymerase chain reaction (PCR) was applied to verify the expression of genes.Results The top five potential antifungal compounds in P. frutescens screened by network pharmacology are: progesterone, luteolin, apigenin, ursolic acid and rosmarinic acid. In vitro antifungal assays showed that rosmarinic acid had a favorable inhibitory effect on fungi. The transcriptomic findings exhibited that the differentially expressed genes of fungus after rosmarinic acid intervention were mainly enriched in the carbon metabolism pathway, while the proteomic findings suggested that rosmarinic acid could inhibit the average growth of Trichophyton mentagrophytes by interfering with the expression of enolase in the glycolysis pathway. Comparison of real-time PCR and transcriptomics results showed that the trends of gene expression in glycolytic, carbon metabolism and glutathione metabolic pathways were identical. The binding modes and interactions between rosmarinic acid and enolase were preliminary explored by molecular docking analysis.Conclusion The key findings of the present study manifested that rosmarinic acid, a medicinal compound extracted from P. frutescens, had pharmacological activity in inhibiting the growth of Trichophyton mentagrophytes by affecting its enolase expression to reduce metabolism. Rosmarinic acid is expected to be an efficacious product for prevention and treatment of dermatophytes.
研究领域:
药理学
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真菌学
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网络药理学
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生物信息学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ziwen Wang,Dongdong Wang,Jiaxuan Fang,Zixin Song,Jiman Geng,Jianfei Zhao,Yifan Fang,Changtao Wang,Meng Li
In this study, an ultrasonic-assisted natural deep eutectic solvent (NaDES) was used to extract flavonoids from Perilla frutescens (L.) Britt. leaves. Of 10 tested NaDESs, that comprising D-(+)-glucose and glycerol exhibited the best total flavonoid extraction rate. Response surface methodology (RSM) was used for extraction modeling and optimization, and the total flavonoid content reached 87.48 ± 1.61 mg RE/g DW, which was a significant increase of 5.36% compared with that of 80% ethanol extraction. Morphological changes in P. frutescens leaves before and after extraction were analyzed by scanning electron microscopy (SEM), and the mechanism of NaDES formation was studied by Fourier transform infrared (FT-IR) spectroscopy. Furthermore, 10 flavonoids were identified by UPLC-Q-TOF-MS. In addition, the NaDES extract had better biological activity according to several antioxidant, cyclooxygenase-2 (COX-2) and hyaluronidase (Hyal) inhibition experiments. Moreover, the stability test revealed that the total flavonoid loss rate of the NaDES extract after four weeks was 37.75% lower than that of the ethanol extract. These results indicate that the NaDES can effectively extract flavonoids from P. frutescens leaves and provide a reference for further applications in the food, medicine, health product and cosmetic industries.
研究领域:
药理学
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分析化学
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食品科学
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医学
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化妆品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
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2026
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IF 1.2
Hao Wang,Chengwei Yu,Yanan Sun,Mengke Tian,Bizhen Zhong,Bin Peng,Mingming Hu,Jinlin Li,Zongcai Tu
This study investigated the effects of Perilla frutescens extracts (PFE) prepared with different solvents on the physicochemical and flavor characteristics of grass carp cubes during cold storage. Results demonstrated that the water-based PFE (WE) exhibited the most effective preservation performance, as evidenced by the lowest total volatile basic nitrogen, total viable counts, thiobarbituric acid-reactive substances, highest moisture retention, and optimal maintenance of pH and texture. In contrast, the 70% ethanol-based PFE (EE) displaye d superior color stability and the lowest fishy odor intensity. These findings suggested that PFE, particularly WE, held promising potential as a natural preservative for freshwater fish products.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Xinyu Yang,Tingting Liu,Mengxia Wei,Ru Zhao,Huiyan Gu,Jialei Li,Fengli Chen,Lei Yang
An improved approach was developed to obtain essential oil, rosmarinic acid , and polysaccharides with sucrose stearate as a green additive from Perilla frutescens (L.) Britt. Leaves using microwave-induced hydrodistillation and simultaneous extraction (MHDE). In this process, we realize the rotation of the material through a rotary motor so that the material is uniformly irradiated by microwaves. The influencing factors on MHDE were systematically optimized by single factor and central composite design. Under the obtained optimization conditions, the yields of essential oil, rosmarinic acid, and polysaccharides were 0.64% ± 0.03%, 0.18% ± 0.01%, and 4.35% ± 0.19%, respectively. GC-MS analysis results showed that the main component of essential oil prepared by MHDE was perillaldehyde , which accounted for 51.91% of the total essential oil, followed by caryophyllene , accounting for 15.79%. However, the ratio of perillaldehyde in the essential oil obtained by traditional hydrodistillation and extraction (HDE) was only 40.69%, and the proportion of caryophyllene was only 12.50%. MHDE uses sucrose stearate with a long hydrocarbon chain structure as an additive, which reduces the nonterpene aliphatics in the obtained essential oil.
研究领域:
化学工程
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植物化学
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食品科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）