PHYTOCHEMICAL ANALYSIS
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2019
·Vol. 31
·期 2
·242-251
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IF 1.963
Luwei Fang,Jinfeng Xie,Liangwen Lin,Minglei Tian,Kyung Ho Row
Introduction Ephedrine is a typical compound found in lots of plant species that is used in several medicines for the treatment of asthma and bronchitis. However, excess amounts are harmful to humans, so it needs to be removed. Objective This study developed a multi-phase extraction (MPE) method with a molecular imprinted polymer (MIP) coated ionic liquid (IL)-based silica (SiO 2 @IL@MIP) to simultaneously extract and separate ephedrine from Pinellia ternata , 10 medicines, and urine samples. Methods IL was immobilized on silica. Subsequently, the IL was combined with the functional monomer, followed by the addition of the crosslinker and template. The resulting sorbent was applied to the MPE, and the extraction, washing and elution solvents were evaluated. Results Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) confirmed the synthesis of SiO 2 @IL@MIP. A maximum adsorption amount of 5.76 mg/g was obtained at 30°C at a neutral pH. In MPE, 10.00 mL of methanol could extract all the ephedrine from Pinellia ternata . The interference was removed by washing with 4.00 mL of water, ethanol, and acetonitrile. Finally, 8.00 mL of methanol/acetic acid (99:1, v / v ) was applied as the elution solvent. The following were extracted: 5.50 μg/g of ephedrine from Pinellia ternata , 0.00–46.50 μg/g from the 10 herbal medicines, and 68.70–102.80 μg/mL in the urine samples. Conclusion The proposed method was applied successfully to the simultaneously extraction and separation of ephedrine from plants and medicines. These results are expected to provide important data for the development of new methods for the separation and purification of bioactive compounds.
研究领域:
分析化学
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药学
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植物化学
·
分离科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Zhigui He,Yanfeng Wang,Yan Yan,Shaowei Qin,Huan He,Renjun Mao,Zongsuo Liang
Pinellia ternata (Thunb.) is a famous traditional Chinese medicine with high medicinal value, but its culture is strongly hindered by the continuous cropping obstacles (CCO) which are tightly associated with allelopathic effects. Deciphering the response mechanisms of P. ternata to allelochemicals is critical for overcoming the CCO. Here, we elucidate the response of P. ternata to phenolic acids treatment via physiological indices, cellular approaches, and transcriptome analysis. Phenolic acids showed a significant effect on the growth of P. ternata seedlings, similar to the phenotype of continuous cropping. Cellular analysis demonstrated that phenolic acids remarkably induced root cell death. Physiological analysis revealed that phenolic acids induced the overaccumulated of H2O2 and
O2−
in root cells. However, two exogenous antioxidants (L-ascorbic acid and β-gentiobiose) aid in the scavenging of over-accumulated H2O2 and
O2−
by promoting the antioxidant enzyme activity such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT). Transcriptome analysis demonstrated that differentially expressed genes (DEGs) related to the cell wall degeneration and reactive oxygen species (ROS) metabolism were upregulated by phenolic acid treatment. In addition, downregulated DEGs involved in sucrose and starch metabolism and phenylpropanoid biosynthesis pathways decreased the key metabolites contents. Taken together, phenolic acids caused root cell death by inducing the overaccumulation of H2O2 and
O2−
, and L-ascorbic acid and β-gentiobiose effectively alleviated ROS stress. The present study elucidates the underlying mechanism of the allelopathic effect of phenolic acids, offers valuable information for further understanding the mechanism of CCO, and could contribute to improving guidance for further P. ternata production.Keywords: Pinellia ternata, allelopathic effect, phenolic acids, antioxidant enzyme activity, reactive oxygen species, transcriptome, continuous cropping obstacles
研究领域:
植物科学
·
分子生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Cheng Zhang,Qinghai Zhang,Ming Luo,Qiuping Wang,Xiaomao Wu
Butylated hydroxytoluene (BHT), as an emerging contaminant in ecosystems, has potential influences on animals, aquatic organisms, and public health, and has been proven to be a major allelochemical of Pinellia ternata . In this study, Bacillus cereus WL08 was used to rapidly degrade BHT in liquid culture. Strain WL08 immobilized on tobacco stem charcoal (TSC) particles notably accelerated BHT removal in contract to its free cells, and exhibited excellent reutilization and storage capacities. The optimal removal parameters of TSC WL08 were ascertained to be pH 7.0, 30 °C, 50 mg L −1 BHT and 0.14 mg L −1 TSC WL08. Moreover, TSC WL08 significantly accelerated the degradation of 50 mg L −1 BHT in sterile and non-sterile soils compared to that of free WL08 or natural dissipation, and notably shortened their half-lives by 2.47- or 362.14- fold, and 2.20- or 14.99- fold, respectively. Simultaneously, TSC WL08 was introduced into the continuous cropping soils of P. ternata , which accelerated the elimination of allelochemical BHT, and notably enhanced the photosynthesis, growth, yield, and quality of P. ternata . This study provides new insights and strategies for the rapid in situ remediation of BHT-polluted soils and effective alleviation of P. ternata cropping obstacles.
研究领域:
环境科学
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植物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yan Zhou,Xiaofang Yang,Leshou Zhang,Shaodui Ye,Ya Liu,Ning Lu,Yong Liu,Xiaodong Wen
Developing a fast, simple, and efficient sample pretreatment method is of great practical significance for the analysis of complex matrix samples. In this study, a novel multifunctional “four-in-one” effervescent tablet based on switchable hydrophobic deep eutectic solvent (SHDES) was prepared, which can simultaneously facilitate the in situ synthesis of SHDES and CO 2 generation, phase transition, and phase separation. The effervescent tablet based on the SHDES-assisted microextraction (ETA-SHDES-ME) technique was combined with inductively coupled plasma optical emission spectrometry (ICP-OES) for the determination of Pb 2+ in Pinellia ternata (Thunb.) Breit. The important parameters affecting the extraction efficiency of Pb 2+ were optimized using the Box-Behnken design (BBD). The enrichment factor (EF) and limit of detection (LOD) of the established method for Pb 2+ are 31 and 0.2 μg/L, respectively, offering a fast, simple, and eco-friendly approach, and has the application potential of field analysis.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）