Journal of Integrative Agriculture
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2018
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IF 1.042
Zhen-gang LIU,Huan-huan WANG,Chao-bin XUE
Laccase (EC 1.10.3.2) is known to oxidize various aromatic and nonaromatic compounds via a radical-catalyzed reaction, which generally includes two types of laccase, Lac1 and Lac2. Lac1 oxidizes toxic compounds in the diet, and Lac2 is known to play an important role in melanizing the insect exoskeleton. In this study, we cloned and sequenced the cDNA of the diamondback moth, Plutella xylostella Lac2 ( PxLac2 ), from the third instar larvae using polymerase chain reaction (PCR) and rapid amplification of cDNA ends techniques. The results showed that the full-length PxLac2 cDNA was 1 944 bp long and had an open reading frame of 1 794 bp. PxLac2 encoded a protein with 597 amino acids and had a molecular weight of 66.09 kDa. Moreover, we determined the expression levels of PxLac2 in different stages by quantitative PCR (qPCR). The results indicated that PxLac2 was expressed differently in different stages. We observed the highest expression level in pupae and the lowest expression level in fourth instar larvae. We also investigated the enzymatic properties of laccase, which had optimal activity at pH 3.0 and at 35°C. Under these optimal conditions, laccase had a Michaelis constant ( K m ) of 0.97 mmol L −1 , maximal reaction speed ( V m ) of 56.82 U mL −1 , and activation energy ( E a ) of 17.36 kJ mol −1 to oxidize 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt). Type II copper enhanced laccase activity below 0.8 mmol L −1 and reduced enzyme activity above 0.8 mmol L −1 with an IC 50 concentration of 1.26 mmol L −1 . This study provides insights into the biological function of laccase.
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高影响因子
PEST MANAGEMENT SCIENCE
·
2020
·Vol. 76
·期 12
·4036-4045
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IF 3.75
Yifan Li,Hong Sun,Zhen Tian,Xinxin Su,Yue Li,Xuan Ye,Yifei Zhou,Shengli Zheng,Jiyuan Liu,Yalin Zhang
BACKGROUND Insect glutathione S-transferases (GSTs) play a crucial role in insecticide detoxification. However, there remains a distinct lack of information regarding the role of GSTs in the detoxification of Tolfenpyrad (TFP) in insects. RESULTS Real-time quantitative PCR showed significant upregulation of PxGSTs after exposure to TFP for 6 h. An in vitro inhibition assay showed that TFP could inhibit PxGSTδ, PxGSTε and PxGSTσ, and the most pronounced inhibitory effect was on PxGSTσ. Metabolism assays displayed that PxGSTσ was superior to other test PxGSTs in metabolizing TFP. The molecular docking of TFP and PxGSTσ revealed that the H-bond provided by the sidechains of Tyr107 and Tyr162 were key to the detoxification of TFP by PxGSTσ. Further tests using mutant PxGSTσ proteins at the sites of Tyr107 (PxGSTσY107A) and Tyr162 (PxGSTσY162A) corroborated that the individual replacement of Tyr107 and Tyr162 could greatly weaken the binding and metabolic abilities to TFP. CONCLUSION Metabolic interactions between the Plutella xylostella (L.) GSTs (PxGSTs) and TFP were deciphered. This study illustrates the molecular metabolism mechanism of PxGSTσ towards TFP and provides theoretical underpinnings for the design and optimization of novel TFP-like insecticides. © 2020 Society of Chemical Industry
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Yifan Li,Ruichi Li,Hainan Shao,Zhuoda Liu,Xueyi Gao,Zhen Tian,Yalin Zhang,Jiyuan Liu
The widespread use of benzoylurea insecticides (BUs) has led to significant resistance issues in various agricultural pests. Previous studies have demonstrated that the overexpression of sigma glutathione S-transferase 1 (PxGSTs1) can confer resistance to novaluron in Plutella xylostella; however, the underlying molecular mechanism remains unclear. This study investigates the role of glutathione S-transferase PxGSTs1 in mediating resistance to BUs in P. xylostella. Using a combination of RNA interference and transgenic Drosophila models, we demonstrated that the overexpression of PxGSTs1 significantly contributes to the resistance against BUs. Functional assays revealed that PxGSTs1 binds to these insecticides with varying affinities. Structural analysis through homology modeling and molecular docking identified the importance of hydrogen bonding and pi–pi stacking in resistance mechanisms. Site-directed mutagenesis confirmed the critical role of Ser65 and Tyr97 in these interactions. Our findings provide a molecular basis for the development of novel BUs and inform strategies for managing BU resistance in P. xylostella.
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高影响因子
PEST MANAGEMENT SCIENCE
·
2018
·Vol. 75
·期 4
·1045-1055
·
IF 3.249
An-jia Wang,Kai-Xin Zhang,Yu-lin Gao,Ai-zhen Weng,Liu-yang Wang,Yun-hui Zhang,Zhi Zhang,Dong-mei She,Jun Ning,Xiang-dong Mei
BACKGROUND Diamondback moth, Plutella xylostella L., is a very important pest of cruciferous vegetables causing excessive economic losses worldwide. Bioactivities of halo-, diazo-, and cyclopropane acetates of P. xylostella sex pheromone have been evaluated using electrophysiology and enzyme inhibition assays. RESULTS A total of 23 sex pheromone analogs of P. xylostella were designed and synthesized and the result shows that (11 Z )-hexadec-11-en-1-yl 2,2,2-trifluoroacetate, (11 Z )-hexadec-11-en-1-yl 2,2,3,3,3-pentafluoropropanoate, and (11 Z )-hexadec-11-en-1-yl trifluoromethanesulfonate elicited potential inhibitory effects at all doses tested in the electrophysiology and enzyme inhibition assays. Interference of locating the sex pheromone source was found strongest when these three analogs were mixed with the sex pheromone at a 10:1 ratio. In addition, field test showed that the rate of mating disruption was over 90% when (11 Z )-hexadec-11-en-1-yl 2,2,2-trifluoroacetate or (11 Z )-hexadec-11-en-1-yl 2,2,3,3,3-pentafluoropropanoate was mixed with the sex pheromone at a 10:1 ratio. CONCLUSION Two sex pheromone antagonists were screen out by electrophysiology, enzyme inhibition assays, wind tunnel and field tests. We believe that these antagonists could be used to establish a novel eco-friendly measure to control P. xylostella and provide evidence for clarifying the specific functions and molecular mechanisms of sex pheromone antagonists. © 2018 Society of Chemical Industry
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Advanced Agrochem
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2026
·
IF 9.2
Li Sun,Yue Peng,Yanyan Gao,Jiale Zhang,Chen Qin,Yue Gao,Demei Xing,Cong Zhou,Jiagao Cheng
A triple-responsive nanopesticide was developed for precise pesticide delivery. • A high pesticide loading content of 33.0% was achieved. • PH, redox and amylase triggered controlled release. • Superior and long-term activity against Plutella xylostella. • Low toxicity to non-target organisms and safe to crops. Traditional pesticide nanocarriers often have limited pore sizes and pore volumes, which can restrict pesticide loading and hinder precise control over release, which in turn affects pest control efficacy. In this study, dendritic mesoporous silica nanoparticles (DMSNs) with large mesopores and high pore volume were used as pesticide carriers. Bis[3-(triethoxysilyl)propyl] tetrasulfide (TESPT) was co-condensed into the silica framework to introduce redox-cleavable tetrasulfide bonds, yielding tetrasulfide-functionalized DMSN (SS-DMSN). Chlorfenapyr was loaded into the dendritic mesopores, and the particle surface was capped with carboxymethyl-β-cyclodextrin (CM-β-CD), thereby constructing a triple-stimuli-responsive pesticide nanodelivery system (CF@SS-DMSN@CD) responsive to pH, redox conditions and amylase. Morphological and physicochemical characterization indicate that CF@SS-DMSN@CD was successfully prepared and exhibits a high pesticide loading content (33.0%). Release behavior studies demonstrate that this delivery system enables on-demand controlled release of the pesticide under alkaline pH, reducing conditions, and in the presence of amylase. Meanwhile, the CM-β-CD layer significantly improves the wetting and spreading behavior of droplets on leaf surfaces. In vivo fluorescence distribution studies revealed pronounced intestinal accumulation and broader distribution of FITC-labeled CF@SS-DMSN@CD in diamondback moth larvae, while insecticidal bioassays demonstrated improved and prolonged insecticidal activity. Safety evaluation results showed that this delivery system exhibits low toxicity to non-target organisms (zebrafish and earthworms) and has no significant adverse effects on soil microbial community structure or crop growth. Overall, this study combines the mesoporous structural advantages of DMSN with the triple-stimuli-responsive properties synergistically regulated by the tetrasulfide bond and CM-β-CD, providing a feasible design approach for the development of highly efficient, precise, and environmentally friendly pesticide nanodelivery systems. Download: Download high-res image (447KB) Download: Download full-size image
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Micro & Nano Letters
·
2022
·Vol. 17
·期 2
·42-48
·
IF 0.98
Liya Zeng,Zhe Wang
The chemical compositions of Ginkgo biloba exocarp extracts (GBEE) and leaf extracts are similar, however, studies describing the development of GBEE biopesticides are few. In this study, the authors investigated the preparation of GBEE nanoemulsionand and characterized by transmittance, physical stability, droplet size and size distribution for the first time. Insecticidal activity and lipophilicity screening of four different solvents indicated ethyl acetate was satisfactory. Based on dispersibility, centrifugation, heating-cooling cycle, cloud point and long-term stability experiments, two formulations (ethyl acetate/Tween 40-Span 60 (9:1)/water and ethyl acetate/Tween 60-Span 60 (9:1)/water) were optimum to formulate nanoemulsions. However, the smallest droplet size and size distribution (10.93 ± 0.16 nm, PDI = 0.15 ± 0.01) was obtained using the Tween 60/Span 60 combination. The insecticidal activity of GBEE nanoemulsion was evaluated against the third instar larvae of Plutella xylostella . Lethal concentration (LC 50 ) values of the optimized nanoemulsion were 127.38 mg/L at 12 h and 72.80 mg/L at 24 h, respectively. This indicated that GBEE nanoemulsion had higher insecticidal activity than the GBEE. The results proposed that the GBEE nanoemulsion is a potential and effective natural alternative for the management of P. xylostella .
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JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Yifan Li,Hong Sun,Zhen Tian,Yue Li,Xuan Ye,Ruichi Li,Xinyu Li,Shengli Zheng,Jiyuan Liu,Yalin Zhang
The long-term and excessive use of insecticides has led to severe environmental problems and the evolution of insecticide resistance in insects. Carboxylesterases (CarEs) are important detoxification enzymes conferring insecticide resistance on insects. Herein, the detoxification process of Plutella xylostella (L.) carboxylesterase 6 (PxEst-6), one representative P. xylostella carboxylesterase, is investigated with cypermethrin, bifenthrin , cyfluthrin and λ-cyhalothrin. RT-qPCR shows that PxEst-6 is highly expressed in the midgut and cuticles of the third instar larvae. Exposure to pyrethroid insecticides resulted in PxEst-6 up-regulation in a short time. Metabolic assays indicate that PxEst-6 has the capacity to metabolize these pyrethroid insecticides. The combination of molecular docking, binding mode analyses and alanine mutations demonstrated that His451, Lys458 and Gln431 were key residues of PxEst-6 for metabolizing pyrethroids and the acetate groups derived from pyrethroids were key sites for being metabolized by PxEst-6. H451- and K458-derived hydrogen bond (H-bond) interactions with the pyrethroid acetate groups and the polar interactions with the pyrethroid acetate group provided by the Q431 sidechain were crucial to the pyrethroids’ metabolism by PxEst-6. Our study contributes to revealing the reasons for pyrethroid resistance in P. xylostella , and provides a fundamental basis for the development of novel pyrethroid insecticides.
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高影响因子
JOURNAL OF APPLIED ENTOMOLOGY
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2023
·Vol. 147
·期 7
·511-519
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IF 1.9
Xi-Zhong Yan,Zhi-Yu Wang,Li Ma,Cheng-Fei Song,Jin-Yu Zhao,Chen-Zhu Wang,Chi Hao
Plutella xylostella Linnaeus (Lepidoptera: Plutellidae) is one of the most important pests of cruciferous vegetables. Mechanically damaged Mentha spicata Linnaeus (Lamiales: Lamiaceae) strongly repels P. xylostella adults; however, the repellent mechanism remains unclear. In this study, the repellent mechanism was explored based on the electroantennogram (EAG) and behavioural responses of P. xylostella to volatiles from the damaged M. spicata . First, the active volatile compounds in the headspace volatiles of damaged M. spicata were identified by gas chromatography-electroantennographic detection (GC-EAD) and gas chromatography–mass spectrometry (GC–MS). Furthermore, the EAG and behavioural responses of P. xylostella to these compounds were investigated. The GC-EAD results indicated that four compounds, 2,4-dimethylheptane, 4-methyloctane, 2-methylnonane, and myrcene induced EAG responses in female antennae. 2-methylnonane and myrcene elicited strong dose-dependent EAG responses starting at a dose of 1 μg/μL. Choice tests in a four-arm olfactometer showed significant repellent effects of the four compounds on P. xylostella adults at 100 μg/μL, and the repellent indices of 2,4-dimethylheptane, 4-methyloctane, 2-methylnonane, and myrcene were 70.0%, 76.7%, 66.7%, and 73.3% to mated females, respectively. Finally, oviposition repellence assays showed that female adults were significantly repelled by these compounds, and the oviposition repellent index for myrcene was 23.9%. 2,4-dimethylheptane, 4-methyloctane, 2-methylnonane, and myrcene mediate the repellence of wounded M. spicata to P. xylostella adults, suggesting that the four compounds contribute towards developing behavioural regulators of this important pest.
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PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 7
·3224-3232
·
IF 4.845
Xi-le Deng,Lei Guo,Hai-hao Ma,Xue-ping Hu,Xiao-mao Zhou
BACKGROUND The diamondback moth ( Plutella xylostella ) is one of the most destructive lepidopteran pests on cruciferous vegetables. However, resistance has emerged to current chemical and biological insecticides used for P. xylostella control, indicating the necessity of screening new targets on P. xylostella , and finding new insecticides against P. xylostella . In particular, octopamine receptors are representative G protein-coupled receptors found only in invertebrates and are potential targets for identifying novel insecticides. RESULTS A β-adrenergic-like octopamine receptor gene ( PxOA2B1 ) was cloned, and its pharmacological characteristics in P. xylostella were studied. The results demonstrated that octopamine could activate the PxOA2B1 receptor, with a half-maximal effective concentration (EC 50 ) of 49.5 n m . Amitraz, an insecticide and acaricide, and its metabolite ( N -2,4-dimethylphenyl- N ′-methylformamidine; DPMF) were also found to act as PxOAB1R agonists. We synthesized phenyl imidazolidin-2-one derivatives 3a–h using DPMF as the lead compound, and compounds 3a–h showed similar antagonist activities as phentolamine, mianserin and chlorpromazine. In particular, 3d , with an EC 50 of 25.2 n m , showed very similar antagonist activity to mianserin. CONCLUSION This research found that PxOAB1R might be a potential target for P. xylostella control. Phenyl imidazolidin-2-ones could be novel potential antagonists targeted at octopamine receptors and would be useful tools for the design and development of novel insecticides. © 2021 Society of Chemical Industry
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Biology-Basel
·
2025
·Vol. 14
·期 12
·1746
·
IF 3.5
Shuhui Fu,Fangyuan Li,Xizhong Yan,Chi Hao
Phytophagous insects rely on highly evolved chemosensory systems to recognize plant cues, which guide essential behaviors such as feeding and oviposition. While the antennal chemosensory functions of Plutella xylostella are well-studied, little is known about the role of its legs in detecting host plant chemicals. This study systematically identified and characterized chemosensory-related genes expressed in the legs of P. xylostella using transcriptome data. A total of 114 leg-expressed chemosensory genes were identified, with PxylCSP9 showing high expression in legs and strong, broad-spectrum binding to key cruciferous plant volatiles. Leg chemosensory proteins, particularly PxylCSP9, play an important role in the chemical perception. Plutella xylostella , a major pest of cruciferous vegetables, depends predominantly on chemoreception to locate host plants. Legs are crucial in insect chemical perception, particularly during close-range and contact chemoreception. However, the molecular basis underlying the chemosensory repertoire in P. xylostella legs remains elusive. To address this, we sequenced chemosensory-related genes in diamondback moth legs. Sequencing identified 32 odorant binding protein (OBP), 18 chemosensory protein (CSP), 26 odorant receptor (OR), 20 gustatory receptor (GR), 15 ionotropic receptor (IR), and 3 sensory neuron membrane protein (SNMP) genes. Comparative analysis with antennal transcriptome data revealed three CSPs, seven ORs, and two GRs newly identified in the legs. Transcriptome analysis showed higher fragments per kilobase of transcript per million mapped reads values for CSPs than for other chemosensory-related gene families. Furthermore, qRT-PCR confirmed the highest expression of PxylCSP9 in the legs, suggesting its role in perceiving external compounds. Fluorescent binding assays revealed high binding affinity of PxylCSP9 for several host plant semiochemicals. Molecular docking predicted a hydrophobic binding pocket in PxylCSP9 with Met11, Leu13, and Leu43 frequently participating in ligand interactions. Our findings indicate that leg-enriched PxylCSP9 is pivotal for host plant recognition during close-range chemoreception, suggesting its potential as a molecular target for precision management through behavior-based strategies.
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高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
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2022
·
IF 1.58
Chengfei Song,Jinyu Zhao,Ruirui Zheng,Chi Hao,Xizhong Yan
The diamondback moth (DBM), Plutella xylostella (L.), is a globally destructive pest of cruciferous vegetables. Excessive use of synthetic pesticides to control this species results in negative effects on the environment, human health, and nontargeted organisms. The essential oils (EOs) derived from plants may be developed as effective alternatives to conventional pesticides. In this study, thirteen EOs were extracted by hydrodistillation, respectively. Their chemical compositions were identified by gas chromatography-mass spectrometry (GC–MS). Furthermore, the biological activities of EOs such as toxicity, antifeedant activity, and growth inhibition effect, toward DBM larvae were investigated. Against DBM second-instar larvae, the most toxic EO was Pelargonium graveolens (LC 50 = 0.36 μg/μl) after 72 hr of exposure, followed by Polygonum hydropiper (LC 50 = 0.53 μg/μl). The Ocimum basilicum EO exhibited the highest antifeedant effect to third-instar larvae at at all set concentrations. At 15 μg/μl, the EOs of Acorus calamus , O. basilicum , and P . graveolens completely inhibited the feeding activities of larvae (100%). The Ruta graveolens EO showed the lowest relative growth rate and the highest growth inhibition rate towards third-instar larvae at diverse concentrations. And the EOs of P. hydropiper , A. calamus , and O. basilicum showed promising growth inhibition activities. Overall, the five EOs ( P . graveolens , O. basilicum , R. graveolens , P. hydropiper , and A. calamus ) showed moderate to high bioactivity, whereas eight EOs were found to be less active against DBM larvae. These results indicate that the five tested EOs are promising to be developed as novel botanical insecticides to control DBM population.
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JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2023
·
IF 1.5
Chengfei Song,Xinyao Ma,Jiao Liu,Li Ma,Xingtao Qie,Xizhong Yan,Chi Hao
The diamondback moth (DBM), Plutella xylostella (L.), is a cosmopolitan obligate pest of cruciferous crops. Currently, DBMs management is largely dependent on broad-spectrum synthetic pesticides, which has led to the adverse effects on the environment, human health, and nontargeted organisms. Thus, alternative control methods are needed. The essential oils (EOs) derived from bioactive species may be developed as effective alternatives for the management of DBMs that is safe and eco-sustainable than conventional pesticides. In this study, we investigated electrophysiological (EAG) and behavioral responses of DBM adults to the thirteen non-host EOs of Acorus calamus , Ageratum conyzoides , Artemisia argyi , Chenopodium ambrosioides , Cymbopogon citratus , Foeniculum vulgare , Ocimum basilicum , Pelargonium hortorum , Perilla frutescens , Pogostemon cablin , Polygonum hydropiper , Rosmarinus officinalis , and Ruta graveolens in order to contribute to the development of low environmental impact management alternatives. EAG recordings revealed all the tested EOs significantly elicited EAG responses of DBM females, and the males were sensitive to the eleven EOs except A. conyzoides and O. basilicum . Meanwhile, EAG recordings of DBMs exhibited dose-dependent responses to these tested EOs in most cases. In Y-tube olfactometer bioassays, the EOs of A. calamus , C. citratus , P. hortorum , P. hydropiper , and R. graveolen demonstrated prominent repellent effects on DBM females at concentrations of 10 μg/μL or higher. The results of oviposition bioassays found the number of eggs on the two plants with the five tested EOs were less than that on the two plants with paraffin oil. And the oviposition indexes of the plants that fixed with the EOs of P. hortorum , R. graveolen , A. calamus , P. hydropiper , C. citratus , and paraffin oil were –71.81%, –59.43%, –57.46%, –51.11%, –35.41%, and 0.09%, respectively. These findings indicate that the five EOs, especially P. hydropiper EO, provide active and safe tools for natural repellents to prevent and control DBMs infestations of cruciferous vegetables.
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危险品化学品经营许可证（带存储）