Food Bioscience
·
2025
·
IF 5.9
Xianjiang Ye,Ran Yang,Zhizhi Yang,Baote Huang,Talha Riaz,Chao Zhao,Jicheng Chen
Food-derived peptides with angiotensin-I-converting enzyme inhibitory (ACEI) activity have garnered significant research attention. This study focused on examining ACEI peptides from Porphyra haitanensis and investigating their potential inhibitory mechanisms through a combination of in silico and in vitro methods. Five novel potential ACEI peptides (ASF: Ala-Ser-Phe, EIIL: Glu-Ile-Ile-Leu, LCVPR: Leu-Cys-Val-Pro-Arg, LDVVN: Leu-Asp-Val-Val-Asn, and LSPSW: Leu-Ser-Pro-Ser-Trp) were identified through a combination of peptidomics and in silico bioinformatics analysis, of which LSPSW showed the highest activity (IC 50 : 5.27 ± 0.53 μM). After simulated gastrointestinal digestion (SGD), the activities of LCVPR and LSPSW remained almost unchanged. The results from inhibition kinetics and molecular docking demonstrated that ACEI peptides with differing inhibition mechanisms could successfully bind to ACE through hydrogen bonds. Based on network pharmacology analysis and cellular experiments, LSPSW exerted antihypertensive effects by regulating the expression levels of peroxisome proliferator activated receptor gamma (PPARG), prostaglandin-endoperoxide synthase 2 (PTGS2), and renin (REN). This research provided a novel theoretical foundation for the high-value application of P. haitanensis .
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Peilin Chen,YuShan Huang,Hongliang Zeng,Mingjing Zheng,Juanjuan Guo
This study systematically investigated the modulatory effects of Porphyra haitanensis polysaccharides (PHP) and its two P. haitanensis polysaccharide components (PHP40 and PHP80) on gut microbiota in ovalbumin (OVA)-sensitized mice via in vitro fermentation. PHP40 and PHP80 exhibited sulfate contents of 5.94 ± 0.05 % and 11.72 ± 0.03 %, respectively, with galactose and glucose as dominant monosaccharides. The different polysaccharide components had different effects on the aforementioned changes in the species composition and structure of the gut microbiota in allergic mice, with distinct dominant microbial profiles across groups. PHP could promote Bacillus and Enterococcus proliferation while inhibiting Staphylococcus . PHP40 and PHP80 could promote Enterococcus and Enterobacter growth but suppressed Staphylococcus and Bacillus . Functional prediction indicated PHP significantly improved galactose metabolism and primary/secondary bile acid biosynthesis, potentially alleviating allergic responses.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yujia Ou,Binhui Zhou,Jin Dong,Tao Hong,Zedong Jiang,Hui Ni,Jun Lu,Mingjing Zheng
Temperature-controlled SE was applied to modulate SDF from P. haitanensis . • Moderate SE increased SDF yield and elevated acidic sugar contents. • SE at 140 °C–160 °C generated porous networks and exposed functional groups. • Functional properties improved under moderate SE and gradually declined at 180 °C. Steam explosion (SE) at different temperatures was applied to modify the structural and functional properties of soluble dietary fiber (SDF) from Porphyra haitanensis . SE treatment at 140 °C promoted partial depolymerization, reduced molecular weight, and increased the SDF yield (by 123.75%), together with significant increases in galactose (by 56.95%), galacturonic (by 66.86%), and glucuronic acids (by 73.84%) ( p < 0.05). Meanwhile, SE-treated P. haitanensis SDF showed a looser and more porous-like microstructure, accompanied by changes in hydration-related behavior. These structural changes were associated with increases in water-holding, swelling, and oil-holding capacities, as well as glucose and cholesterol adsorption capacities and inhibitory activities against α-amylase, α-glucosidase, and pancreatic lipase. In contrast, SE treatment at 180 °C was associated with partial structural reorganization and less pronounced functional improvements. Overall, controlled SE temperature can provide a green and efficient strategy for enhancing the physicochemical and biological functionalities of seaweed-derived SDF.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Mingjing Zheng,Xin Tian,Zhipeng Li,Tao Hong,Yanbing Zhu,Yuanfan Yang,Qingbiao Li,Hui Ni,Zedong Jiang
Porphyra haitanensis polysaccharides (PHP) has potential antioxidant and hypolipidemic activities, but still needs improvement. Therefore, ultra-high pressure (UHP) assisted extraction was applied to modify the structure, antioxidant, and hypolipidemic activities of PHP. UHP assisted extraction increased the total sugar, uronic acid, and 3,6-anhydro-ʟ-galactose contents of PHP, which increased by 15.85%–16.12%, 18.95%–24.32%, 20.54%–23.66% with 500–600 MPa UHP, respectively. Meanwhile, UHP modified PHP became more rough and porous than native PHP. Besides, UHP assisted extracted PHP showed better in vitro antioxidant and hypolipidemic abilities. Especially, 500–600 MPa UHP increased 72.43%–86.42% 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacities, 12.32%–12.82% pancreatic lipase inhibitory ability, and 14.93%–15.23% glycocholate binding abilities of PHP ( p < 0.05). Moreover, UHP assisted extracted PHP greatly decreased the lipid droplet and triglyceride contents of 3T3-L1 adipocytes ( p < 0.05). Our findings can provide the theoretical basis for the high value utilization of Porphyra haitanensis and its polysaccharides with UHP modification.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Yujia Ou,Yuehong Guo,Lijingting Xu,Xiaodan Lu,Zebin Guo,Baodong Zheng
In this study, two glycoproteins were extracted from porphyra haitanensis by ammonium sulfate and ethanol extraction methods, and their differences in structure and hypoglycemic activity were compared. The results showed that the protein, amino acid , and galactose contents of the glycoprotein (PG) extracted using an ammonium sulfate method were higher than those of glycoprotein (PP) extracted from ethanol. PG inhibited both α-amylase and α-glucosidase, substantially reduced in vivo hyperglycemia , regulated the intestinal production of beneficial bacteria ( Lachnospiraceae , Odoribacter, and Lactobacillus ), and increased the abundance of commensal microbial metabolites (e.g., taurine and hypotaurine) via the activation of taurine and hypotaurine , butanoate metabolism, and insulin secretion pathways. PP was superior in regulating lipid metabolism through the AMPK and fatty acid degradation pathways, which was positively correlated with its increased polysaccharide fragment, Lactobacillus and Rikenellaceae_RC9_ gut_group . Our findings shed light on the potential of Porphyra haitanensis glycoproteins as novel natural antidiabetic compounds.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Kit-Leong Cheong,Xu-Ting Xie,Tao Zhou,Sathuvan Malairaj,Suresh Veeraperumal,Saiyi Zhong,Karsoon Tan
Ulcerative colitis is a chronic, spontaneous inflammatory bowel disease that primarily affects the colon. This study aimed to explore how Porphyra haitanensis porphyran (PHP) modulates the immune response and the associated mechanisms that alleviate dextran sulphate sodium-induced colitis in mice. Histological assessments via H&E staining and AB-PAS staining revealed that PHP intervention partially restored the number of goblet cells and improved intestinal mucosal function. Immunohistochemical and Western blot analyses of claudin-1, occludin, and MUC-2 demonstrated that PHP could repair the intestinal barrier and reduce colon damage by upregulating the expression of these proteins. PHP intervention was associated with a decrease in pro-inflammatory cytokine expression and an increase in anti-inflammatory cytokine expression. Moreover, the expression of proteins involved in intestinal immune homing, such as CCR-9, CCL-25, MAdCAM-1, and α4β7, was significantly suppressed in response to PHP treatment. Conversely, PHP upregulates the expression of CD40 and TGF-β1, both of these can promote healing and reduce inflammation in the gut lining. This study demonstrates that PHP can ameliorate ulcerative colitis by enhancing the intestinal barrier and modulating immune responses. These findings offer valuable insights into the potential utility of P. haitanensis as a promising natural product for managing ulcerative colitis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Manling Yu,Hongmei Yin,Jingjing Huang,Xixi Cai,Jiulin Wu,Xiaodan Shi,Shaoyun Wang
Porphyra haitanensis with many bioactive components has great prospects for application in the functional food, pharmaceutical, and cosmeceutical industries. In this study, two sequentially extracted fractions (PhE1 & PhE2) were isolated and structurally characterized. The results showed that PhE1 was mainly composed of protein (36.82%) and neutral sugar (32.43%), while PhE2 mainly contained neutral sugar (58.70%). Both had galactose as main monosaccharides, PhE2 also contained a small amount of glucose. To widen the application of PhEs in starchy foods, they were added to corn starch (CS) system and co-gelatinized. Rheological tests indicated that PhEs could effectively affect gel strength of CS. The addition of PhEs effectively inhibited the content of leached amylose in CS gelatinization system except those at an addition of 5% PhE1 and 5% PhE2. FT-IR data suggested that PhE1 could improve the short-range order, but PhE2 showed no significant effects. SEM images showed that the pore wall of the composites thickened, and the network structure became relatively ordered. In vitro tests, PhEs significantly reduced the contents of rapidly digestible starch, and PhE1 presented better effects. Therefore, PhEs may be used as potential materials to delay the digestion of CS and related products.
✓ 已验证
产品关联
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2026
·
IF 6.9
Yujia Ou,Binhui Zhou,Yesong Wang,Tao Hong,Lijun Li,Zedong Jiang,Hui Ni,Jun Lu,Mingjing Zheng
Developing efficient modification strategies for seaweed-derived insoluble dietary fiber (IDF) is essential for improving its functional performance. In this study, steam explosion (SE) was applied to investigate the temperature-dependent structural and functional responses of Porphyra haitanensis IDF. Moderate SE treatment within the 140 °C–160 °C interval transformed the compact IDF matrix into a looser and more porous structure, accompanied by increasing crystallinity, thermal stability, and hydrophilicity. Compared with the control, SE140 and SE160 increased water-holding capacity by 12.73% and 21.79%, swelling capacity by 36.94% and 50.45%, and oil-holding capacity by 68.13% and 28.83%, respectively ( p < 0.05). In vitro cholesterol adsorption capacity and pancreatic lipase inhibitory activity were also enhanced. Combined structural observations and principal component analysis supported the association among moderate SE-induced structural loosening, enhanced water immobilization, improved hydration properties, and increased in vitro lipid-related functional indicators. In contrast, SE at 180 °C caused partial structural collapse and weakened the improvement in functional performance. These results suggest that moderate SE, particularly within the 140 °C–160 °C interval, may serve as a green and scalable strategy for improving seaweed-derived IDF as a functional food ingredient.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）