RSC Advances
·
2023
·Vol. 13
·期 31
·21633-21642
·
IF 3.9
Yize Wang,Hua Kang,Jao Hu,Heming Chen,Huimin Zhou,Ying Wang,Huizhen Ke
In this study, we prepared a polyacrylonitrile (PAN) composite nanofiber membrane comprising Portulaca oleracea L. extract (POE) and a zinc-based metal–organic framework (MOF) by an in situ growth method as a potentially new type of wound dressing with a slow drug-release effect, to solve the problem of the burst release of drugs in wound dressings. The effects of the MOF and POE doping on the nanofiber membranes were examined using scanning electron microscopy (SEM) and FTIR spectroscopy. SEM analysis revealed the dense and uniform attachment of MOF particles to the surface of the nanofiber membrane, while FTIR spectroscopy confirmed the successful fusion of MOF and POE. Furthermore, investigations into the water contact angle and swelling property demonstrated that the incorporation of the MOF and POE enhanced the hydrophilicity of the material. The results of the in vitro release test showed that the cumulative release rate for PAN/MOF/POE60 decreased from 66.5 ± 2.34% to 32.18 ± 1.31% in the initial 4 h and from 90.54 ± 0.79% to 65.92 ± 1.95% in 72 h compared to PAN/POE, indicating a slowing down of the drug release. In addition, the antimicrobial properties of the fiber membranes were evaluated by the disc diffusion method, and it was evident that the PAN/MOF/POE nanofibers exhibited strong inhibition against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The antioxidant properties of the nanofiber membranes loaded with POE were further validated through the DPPH radical scavenging test. These findings highlight the potential application of the developed nanofiber membranes in wound dressings, offering controlled and sustained drug-release capabilities.
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FIBERS AND POLYMERS
·
2024
·
IF 2.2
Zhang Qi,Yang Ce,Gu Mingqi,Qin Shaoxuan,Yin Jianhua,Wang Xiangrong,Zhang Wei
The integration of natural dyes and biomass-based dyeing auxiliaries holds huge potential to address the environmental sustainability challenges, which the textile industry encounters. This research employed Portulaca oleracea L. extract as a natural dye, utilizing laccase as a catalyst for polymerizing the dye, thereby creating a precursor that enhances its eco-friendly dyeing attributes on cotton fabrics. The initially exhibited suboptimal direct dyeing performance on cotton fabrics, resulting in a faint yellow hue and a K/S value of 0.66. However, following laccase catalysis, the depth of color on the dyed fabric significantly increased, with a K/S value reaching 4.41, and the dye uptake rate increased from 14.54% to 33.69%. The resulting dyed fabric exhibited excellent fastness to soap washing, light, and rubbing, all scoring above levels 4 to 5. Analysis of dyeing kinetics and adsorption models indicated that the dyeing process was consistent with both the pseudo-first-order and pseudo-second-order kinetic models, suggesting physical and chemical bonding between the precursor and the fiber. Further investigations employing ultraviolet spectrum analysis, matrix-assisted laser desorption/ionization time of flight mass spectrometry, and gas chromatography-mass spectrometer revealed the fundamental composition of the natural dye, the molecular weight, and structural characteristics of the precursor, substantiating that the catalytic mechanism involves laccase-catalyzed oxidation. Additionally, the dyed cotton yielded an ultraviolet protection factor value of 230 and a long-lasting herbal fragrance, accurately identified by an electronic nose. These results confirm the viability of intensifying fabric color using the laccase-catalyzed system. This technique significantly expands the environmental applications of its extract.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Xiaomin Zhao,Xingyu Ren,Haoming Li,Bin Zhang,Xiaojing Fan,Xianchao Feng
Development of effective, safe, and degradable food packaging is essential to meet the demands of consumers and to ensure the continued growth of the food industry. In this study, superabsorbent bioactive aerogels based on cellulose and polyvinyl alcohol combined with the antibacterial bioactive extracts extracted from Portulaca oleracea were fabricated for the preservation of chilled meats. The main physicochemical and mechanical properties of the bioactive aerogels were characterized and evaluated. The aerogels had remarkable water and oil sorption abilities and excellent antibacterial capacities, which could significantly delay spoilage of the chilled meat. Compared with commercialized absorbent pads, the bioactive aerogels effectively delayed color changes and retarded the lipid oxidation of meat, and decreased the growth of bacterium during the storage time. The microbial community analysis showed that the bioactive aerogels treatments have significant impacts on the microbial flora of chilled meat, which can significantly inhibit the growth of Pseudomonas and Escherichia, two primary spoilage microorganisms. These findings suggested that the bioactive aerogels can effectively extend the shelf-life of chilled meat and have great application potential in novel active packaging
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Nutrients
·
2024
·Vol. 16
·期 20
·3549
·
IF 4.8
Yiming Zhang,Shengying Zhu,Yueming Gu,Yanjing Feng,Bo Gao
Background/Objectives: Hyperuricemia (HUA) is a common metabolic disease caused by purine metabolic disorders in the body.Portulaca oleraceaL. (PO) is an edible wild vegetable. Methods: In this study, the regulatory effect of PO on HUA and its potential mechanism were initially elucidated through network pharmacology and experimental validation. Results: The results showed that PO from Sichuan province was superior to the plant collected from other habitats in inhibiting xanthine oxidase (XOD) activity. Berberine and stachydrine were isolated and identified from PO for the first time by UPLC-Q-Exactive Orbitrap MS. The potential molecular targets and related signaling pathways were predicted by network pharmacology and molecular docking techniques. Molecular docking showed that berberine had strong docking activity with XOD, and the results of in vitro experiments verified this prediction. Through experimental analysis of HUA mice, we found that PO can reduce the production of uric acid (UA) in the organism by inhibiting XOD activity. On the other hand, PO can reduce the body ‘s reabsorption of urate and aid in its excretion out of the body by inhibiting the urate transporter proteins (GLUT9, URAT1) and promoting the high expression of urate excretory protein (ABCG2). The results of H/E staining showed that, compared with the positive drug (allopurinol and benzbromarone) group, there was no obvious renal injury in the middle- and high-dose groups of PO extract. Conclusions: In summary, our findings reveal the potential of wild plant PO as a functional food for the treatment of hyperuricemia.
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GENOMICS
·
2024
·
IF 3.4
Jiahui Li,Yuyang Chen,Shuang Zhang,Yuehan Zhao,Demeng Gao,Jiaying Xing,YuYan Cao,Guangyu Xu
This study investigated the preventive and protective effects of Portulaca oleracea polysaccharides (PP) on Acute liver injury (ALI) in mice and its regulatory effects on intestinal microorganisms, and explored the underlying protective mechanisms. Initially, PP was administered, and then CCl4 was used to induce the mouse ALI model. Serum and liver markers were measured by ELISA. The fecal microbiota was analyzed by 16S rRNA sequencing. The results showed that PP significantly decreased the expression levels of ALT and AST in the serum of mice. The expression levels of MDA, TNF-α, and IL-6 in liver tissue were found to be reduced, while the levels of GSH and SOD increased. At the same time, PP increased the number of Bacteroides , reduced the number of Proteobacteria , activated the GAG degradation pathway, protected the integrity of the intestinal barrier, inhibited oxidative stress and reduced inflammation, thereby assisting the prevention and protection of ALI.
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INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Hui Zhang,Jinpeng Yang,Guilin Chen,Chunlei Yang,Mingquan Guo
Tobacco-Specific Nitrosamines (TSNAs) are a class of common carcinogens that are widely present in cigarette products and smoke, which seriously affect the health of smokers and vastly restrict the safety of tobacco products. In our present research, the effects of Portulaca oleracea on nitrite scavenging and nitrosamine formation were evaluated through in vitro and tobacco field experiments. Further, four compounds Ferulic Acid O -Glucoside, Ellagic Acid , 13:4 + 4O Fatty Acyl Hexoside, and Feruloylmalic Acid were tentatively screened as potential Nitrate Reductase (NR) ligands by affinity ultrafiltration HPLC-MS. Molecular docking was then carried out to further verify the inhibitory activities of these potential ligands and elucidate their interaction mechanisms with NR. The results showed that P. oleracea exhibited substantial abilities in scavenging nitrite and blocking the formation of nitrosamines, which was mainly attributed to its abundant reductive components, especially its phenolics. In conclusion, the current study provided new insight into new strategies to reduce tobacco toxicity based on natural extracts, which is of great value to the tobacco industry.
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Antioxidants
·
2023
·Vol. 12
·期 7
·1400
·
IF 7
Ke Ning,Yameng Duan,Weiwei Tong,Yue Chen,Qinghui Zhang,Qiuhong Xie,Hongyu Xiang
Purslane, a common wild vegetable, contains active substances with various biological functions. However, its effects have been under-investigated in ulcerative colitis (UC). Therefore, this study investigated the therapeutic effects of purslane macromolecular (POEM) and small molecular extracts (POES) on dextran sulfate sodium (DSS)-induced UC in mice. Membrane separation was used to obtain extracts of different molecular weights, and their compositional differences were compared using liquid chromatography–mass spectrometry (LC/MS). POEM contained more proteins and polysaccharides, whereas POES contained more organic acids and alkaloids. These differences in composition were directly responsible for the different degrees of remission of the alleviated UC in model mice. POEM may alleviate UC by regulating the antioxidant capacity and the gut microbiota, whereas the major alleviatory effect of POES was primarily related to the regulation of antioxidant capacity. The POEM and POES effects identified in this study provide a theoretical basis for the development of purslane as a functional food.Keywords:ulcerative colitis;macromolecular extract;purslane;oxidative stress;small molecular extract;gut microbiota;Portulaca oleraceaL.
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International Journal of Medical Sciences
·
2025
·Vol. 22
·期 4
·856-872
·
IF 3.2
Li Bowei,Yang Jingyuan,Wang Zhixing,He Mingzhu,Chen Xujing,Chen Yixuan,Shen Baoying,Chen Junru,Yang Chunqi,Li Tian,Lai Chengcai,Gao Yue,Cai Hong
Introduction: Solar dermatitis, a condition triggered by excessive exposure to ultraviolet B (UVB) radiation, results in inflammatory skin damage marked by erythema, edema, and epidermal injury. Portulaca oleracea (PO) and Patrinia scabiosaefolia (PS) have been traditionally used in dermatological treatments, though their mechanistic pathways in UVB-induced skin injury are not fully understood.Methods: In this study, a mouse model of UVB-induced solar dermatitis was employed to evaluate the therapeutic potential of combined PO and PS (POPS) extracts. After UVB irradiation, POPS extracts were administered, and their bioactive compounds were identified through ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Network pharmacology, molecular docking, and pathway analysis were performed to identify key targets, focusing on the IL-17/CCL2 pathway and oxidative stress reduction.Results: Treatment with POPS extracts significantly diminished UVB-induced inflammation, erythema, and epidermal thickening in a dose-dependent manner. Network pharmacology and docking studies identified curvularin and olmesartan medoxomil as bioactive components with high affinity for IL-17 and IL-17RA targets, modulating the IL-17/CCL2 axis. In vivo experiments demonstrated that POPS extracts suppressed the expression of IL-17 and CCL2, reduced macrophage infiltration, and alleviated oxidative stress, effectively mitigating the symptoms of solar dermatitis.Conclusion: This study provides insight into the anti-inflammatory and protective properties of POPS extracts in solar dermatitis, highlighting their potential as a treatment through IL-17/CCL2 pathway modulation and oxidative stress reduction.
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PHYTOTHERAPY RESEARCH
·
2025
·
IF 6.3
Risheng Zhao,Min Zhang,Gege Yang,Wei Liu,Hui Yu,Shuang Yan,Ting Ren,Linxin Zhang,Mengyang Wang,Haiming Sun
Hypertensive heart failure pathogenesis involves angiotensin II (Ang II)-mediated mechanisms through both hemodynamic overload and direct cellular signaling pathways. This study investigates the therapeutic potential of Portulaca oleracea L. (POL) extract and its active constituent Oleracein E (OE) in attenuating Ang II-induced pathological cardiac remodeling and subsequent heart failure progression. An experimental model of hypertensive heart failure was established in C57BL/6 mice through continuous subcutaneous infusion of Ang II for 4 weeks, with Oleracein E (OE) intervention administered during the final 2 weeks of the protocol. To elucidate the molecular mechanisms underlying OE's cardioprotective effects, we employed an integrated approach combining RNA sequencing analysis, molecular docking simulations, and target validation through drug affinity response target stability (DARTS) and cellular thermal shift assay (CETSA) techniques. The experimental results demonstrated that both POL extract and its active component OE exerted significant cardioprotective effects against Ang II-induced cardiac dysfunction in murine models, primarily through attenuation of pathological cardiac hypertrophy and suppression of inflammatory responses. Transcriptomic profiling via RNA sequencing identified the MAPK signaling pathway as a critical mediator of these protective effects. Subsequent transcription factor analysis revealed STAT2 as a key regulatory component in this pathway. Importantly, OE treatment effectively mitigated inflammatory responses in both in vivo cardiac tissues and in vitro cultured cardiomyocytes by specifically inhibiting the Ang II-activated MAPKs/STAT2 signaling cascade. Genetic ablation experiments further confirmed the essential role of this pathway, as the anti-inflammatory efficacy of OE was completely abolished in cardiomyocytes with MAPKs or STAT2 deficiency. Molecular interaction studies employing in silico docking simulations combined with experimental validation through DARTS and CETSA techniques provided direct evidence of physical binding between OE and the STAT2 protein. Our findings demonstrate that Oleracein E (OE) confers cardioprotection against Ang II-induced myocardial injury through a novel mechanism involving STAT2 targeting and subsequent suppression of MAPK signaling-mediated inflammatory cascades. These results not only elucidate a previously unrecognized pharmacological pathway but also suggest that OE and its derivatives hold significant therapeutic promise for the clinical management of hypertensive heart failure, potentially offering a targeted approach to modulate pathological cardiac remodeling.
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