FOOD RESEARCH INTERNATIONAL
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2025
·
IF 8
Min Li,Jia-Yao Si,Peng-Fei Xie,Zhen-Jie Feng,Sheng-Jie Li,Feng Zhou,Jian-Mei Li
The global aging crisis has increased the risk of atherosclerosis (AS), positioning vascular senescence as a critical therapeutic target. Procyanidin C1 (PCC1), a bioactive polyphenol from hawthorn, demonstrates dual senolytic and longevity-enhancing effects. This study explored the regulatory role and mechanisms of PCC1 in AS using an ApoE −/− mouse model fed a high-fat diet (HFD) and a D-galactose (D-Gal)-induced cellular senescence model. Hawthorn oligomeric proanthocyanidins (HOPCs), as well as PCC1, showed potent anti-atherosclerotic and anti-aging effects by improving serum lipid metabolism and reducing aortic root plaque, vascular senescence markers and inflammatory adhesion molecules. Metabolomic analysis demonstrated that PCC1 significantly suppresses key enzymes in arachidonic acid (AA) metabolism, thereby decreasing downstream pro-inflammatory mediators. Target identification studies, including drug affinity responsive target stability (DARTS) assay, liquid chromatography-tandem mass spectrometry (LC-MS/MS), cellular thermal shift assay (CETSA), and molecular docking, identified heat shock protein 90 (HSP90) as a direct target of PCC1, leading to inhibition of its downstream signaling. In vitro, PCC1 selectively cleared senescent cells, suppressed the senescence-associated secretory phenotype (SASP), enhanced endothelial nitric oxide synthase (eNOS) phosphorylation, and inhibited the toll-like receptor 2 (TLR2)/nuclear factor kappa-B (NF-κB) pathway, demonstrating multimodal anti-aging and anti-inflammatory effects. Together, these findings indicate that PCC1 mitigates atherosclerosis by targeting both metabolic and inflammatory pathways through senescent cell clearance and HSP90-mediated blockade of the TLR2/NF-κB signaling axis. This dual mechanism highlights PCC1 as a promising vasoprotective agent and supports the potential application of hawthorn extracts in functional foods aimed at preventing aging-related cardiovascular diseases.
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PubMed
高影响因子
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
·
2022
·Vol. 78
·期 1
·213-227
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IF 5.08
Zhang Haihua,Lang Wuying,Liu Xin,Bai Jiangsong,Jia Qinghui,Shi Qiumei
Ulcerative colitis (UC) is a recurrent chronic inflammatory disease. The symptom of UC is mainly diarrhea including bloody stools. Increasing evidence has suggested that procyanidin A1 (PCA1) exerts an anti-inflammatory effect in several diseases. However, the role of PCA1 in UC is still a mystery. In our study, we explored the effect of PCA1 in dextran sulfate sodium (DSS)–induced UC mice and lipopolysaccharide (LPS)-stimulated HT-29 and IEC-6 cells. Then, cell proliferation, apoptosis, the production of proinflammatory cytokines, and autophagy-related markers were determined. Furthermore, the AMPK/mTOR/p70S6K signaling pathway was assayed by Western blot assay. In in vivo study, we found that PCA1 administration alleviated DSS-induced UC, as evidenced by reducing weight loss, clinical scores, colon weight/length ratio, histological damage, proinflammatory cytokines, and apoptosis. Moreover, we showed that the expression of Beclin-1 and LC3II/I ratio was increased, whereas the level of p62 was decreased after PCA1 treatment in vivo . Meanwhile, the reduced AMP/ATP ratio, enhanced expression of p-AMPK, and decreased p-p70S6K and p-mTOR levels indicate the activation of AMPK/mTOR/p70S6K signaling pathway. In in vitro study, PCA1 promoted cell proliferation and inhibited cell apoptosis in LPS-stimulated HT-29 and IEC-6 cells. Pro-inflammatory cytokines and autophagy-related factors exhibited the same trend as in in vivo results. Mechanically, PCA1 activated the AMPK/mTOR/p70S6K signaling pathway. The treatment with an AMPK inhibitor compound C significantly reversed the anti-inflammatory effect of PCA1 in LPS-stimulated cells. Taken together, these data indicated that PCA1 alleviated UC through induction of AMPK/mTOR/p70S6K-mediated autophagy.
研究领域:
胃肠病学
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药理学
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免疫学
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细胞生物学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·
IF 3.3
Tian-Xiao Yang,Hang Li,Yuan Zhu,Yu Gao,Hong-Ning Lv,Sheng-Hua Zha,Xiao-Li Sun,Qing-Sheng Zhao
Procyanidins (PC) are formed by the polymerisation of flavan-3-ol monomers, which have excellent bioactivity and present great health benefits. However, the application range of the PC is greatly limited due to their poor stability. Therefore, in this study, starch aerogels were used as carriers to encapsulate PC. After screening, wheat starch aerogel (WSA) was finally chosen to encapsulate PC. Afterward, characterization (SEM, XRD, FT-IR, TGA and BET), stability testing, investigations on antioxidant activity, and in vitro tests simulating digestion were carried out. The results of relevant characterization showed that the micro surface structure of the procyanidin wheat starch aerogel (PC-WSA) presented a network structure, and PC were encapsulated in WSA. In addition, PC-WSA allows PC to be released in the final stage of simulated digestion. Meanwhile, the results of antioxidant experiments indicate that PC-WSA exhibits antioxidant activity after encapsulating PC. Moreover, the retention of PC in PC-WSA was still 53.63 ± 3.23% when stored at 70 °C for 20 days. After 6 days of light exposure, the retention of PC in PC-WSA under different light sources was all above 85%, which was higher than that of PC. In conclusion, the wheat starch aerogel could improve the stability of PC and has great potential for application.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Taotao Dai,Jun Chen,Qian Li,Panying Li,Peng Hu,Chengmei Liu,Ti Li
Procyanidins were reported to have an inhibitory effect on α-amylase, but the interaction mechanism between procyanidins and α-amylase was rarely reported. Spectroscopic and molecular docking techniques were utilized to explore the interaction between porcine pancreatic α-amylase (PPA) and B-type procyanidin dimer (PB2). PB2 decreased the intrinsic fluorescence and surface hydrophobicity of PPA, indicating that an interaction occurred and complex formed. The binding process of complex was spontaneous and the main interaction was hydrophobic force. Circular dichroism showed conformational changes of PPA with an increasing of α-helix and β-sheet structure. Molecular docking speculated that PB2 could form hydrophobic force with PPA by bind to the active sit (Asp 167, Asn 100, Arg 158, His 201). This research can offer new insights into the mechanism of PB2 in inhibiting PPA catalysis and provide useful information on dietary recommendation of PB2 for the treatment of type 2 diabetes.
研究领域:
生物化学
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分子生物学
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药理学
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食品科学
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DOI
PubMed
高影响因子
Materials Today Bio
·
2024
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IF 8.7
Linxiao Sun,Shaoyin Wei,Chenglong Wang,Yipiao Zhang,Xingjie Zan,Lianxin Li,Chunwu Zhang
Chronic inflammatory diseases such as diabetic wounds and osteoarthritis are significant threats to human health. Failure to scavenge longstanding excessive reactive oxygen species (ROS) is an important cause of chronic inflammatory diseases, yet existing treatments that provide long-lasting therapeutic effects are limited. Here, procyanidin capsules were synthesized in a simple one-step way using calcium carbonate as a template. The biosafety of procyanidin capsules in vitro and in vivo was monitored by cytotoxicity and pathological sections. The therapeutic effect of procyanidin capsules in diabetic wounds and osteoarthritis was accessed by pathological evaluation combined with the quantification of inflammatory markers. The data showed that procyanidin capsules could long-term scavenge excessive ROS and effectively promote articular cartilage repair in osteoarthritis, accelerating diabetic wound healing. Lastly, transcriptome analysis suggested that procyanidin capsules commonly regulated adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) signaling in diabetic wounds and osteoarthritis. This study provides a straightforward protocol for creating procyanidin capsules, while presenting a promising new therapeutic option for long-term scavenging ROS in chronic inflammatory diseases.
研究领域:
药理学
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炎症
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生物技术
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伤口愈合
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骨科
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Taotao Dai,Jun Chen,David Julian McClements,Ti Li,Chengmei Liu
Procyanidins are reported to inhibit α-glucosidase, which may be a useful attribute for developing functional foods that control post-prandial blood sugar levels. At present, the nature of the molecular interaction between procyanidins and α-glucosidase is poorly understood. In this study, spectroscopic analyses and computer simulations were used to investigate the interactions between α-glucosidase and B-type procyanidin dimer (BPD). Our results suggest that BPD binds to α-glucosidase through a combination of hydrophobic and hydrophilic interactions. This hypothesis was based on measurements of the intrinsic fluorescence quenching, conformational changes, and surface hydrophobicity of the α-glucosidase after binding. Thermodynamic analysis suggested that α-glucosidase had one binding site for BPD and that the interaction was spontaneous. Homologous modeling of α-glucosidase was used to provide information about the precise nature of the molecular interactions. Molecular docking analysis suggested that BPD formed hydrogen bonds and hydrophobic interactions with α-glucosidase when it bound to its active site. This research offers new insights into the mechanism of interaction between procyanidins and α-glucosidase, which may be useful for the development of functional foods to tackle type 2 diabetes.
研究领域:
生物化学
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分子生物学
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食品科学
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糖尿病研究
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PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2014
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IF 2.235
Miao Liang,Rongxin Su,Wei Qi,Yi Zhang,Renliang Huang,Yanjun Yu,Libing Wang,Zhimin He
Efficient immobilization of catalytic active metal nanoparticles into porous supporting materials is of important scientific interest in practice. We report on the fabrication of novel bionanocomposites, comprising a three-dimensional porous eggshell membrane (ESM) bioscaffold decorated with catalytic active metal (Pt, Pd) nanoparticles, to reduce highly toxic Cr(VI). Procyanidin (Pro), a natural plant polyphenol with abundant phenolic hydroxyls, was first covalently grafted on the ESM fiber surface to provide stable binding sites for chelating metal precursors. Highly dispersed Pt and Pd nanoparticles with small size were facilely generated and stably immobilized onto the surface of ESM followed by NaBH4 reduction. These metal nanoparticle-incorporating ESM composites were active heterogeneous catalysts for the reduction of toxic Cr(VI) to Cr(III) by employing formic acid as the reducing agent. Notably, it is easy to recover and recycle the catalysts, revealing the good stabilization of procyanidin-grafted ESM for nanoparticles.
研究领域:
环境科学
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材料科学
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生物化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qin Geng,David Julian McClements,Zhihua Wu,Ti Li,Xuemei He,Xixiang Shuai,Chengmei Liu,Taotao Dai
Procyanidins are bioactive polyphenols that have a strong affinity to proteins. Beta-lactoglobulin (BLG) is widely used as an emulsifier in the food and other industries. This study evaluated the interaction between BLG and A-type procyanidin dimer (PA2) using the spectroscopic, thermodynamic, and molecular simulation. PA2 decreased the transmissivity and quenched the intrinsic fluorescence of BLG, suggesting that the two molecules formed a complex. The binding of PA2 reduced the surface hydrophobicity and altered the conformation of BLG with increasing the random coil regions. Thermodynamic and isothermal titration calorimetry analyses suggested that the main driving force of PA2-BLG interaction was hydrophobic attraction. Molecular docking simulations were used to identify the main interaction sites and forces in the BLG-PA2 complexes, which again indicated that hydrophobic interactions dominated. In addition, the influence of PA2 on the ability of BLG to form and stabilize O/W emulsions was analyzed. Emulsions formulated using BLG-PA2 complexes contained relatively small droplets (D 4,3 ≈ 0.7 μm) and high surface potentials (absolute value >50 mV). Compared to BLG alone, BLG-PA2 complexes improved the storage stability of the emulsions. This study provides valuable new insights into the formation, properties, and application of protein-polyphenol complexes as functional ingredients in foods.
研究领域:
食品科学
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生物化学
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物理化学
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分子生物学
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PubMed
高影响因子
Food & Function
·
2019
·Vol. 10
·期 2
·765-774
·
IF 3.241
Taotao Dai,Jun Chen,David Julian McClements,Peng Hu,Xiaoqin Ye,Chengmei Liu,Ti Li
Rice glutelin and procyanidins are often used in functional foods as sources of plant-based proteins and polyphenols, respectively, but little is currently known about the interactions between them. In our research, the interaction between rice glutelin and the B-type procyanidin dimer (PB2) was investigated. The presence of the PB2 decreased the α-helix and random coil structure of the rice protein and reduced its surface hydrophobicity. However, the PB2 did not adversely affect the functional performance of RG in emulsions. Conversely, the antioxidant capacity of the PB2 was enhanced in the presence of the rice protein. Fluorescence spectroscopy confirmed that the protein and PB2 formed molecular complexes, which were primarily the result of hydrophobic attractive forces. Molecular docking analysis provides insights into the nature of the interaction between the rice protein and PB2. This study provides valuable insights into the nature of the interactions between plant proteins and polyphenolic nutraceuticals.
研究领域:
食品科学
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营养学
·
生物化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Miao Liang,Rongxin Su,Renliang Huang,Wei Qi,Yanjun Yu,Libing Wang,Zhimin He
Facile, efficient, and robust immobilization of metal nanostructures on porous bioscaffolds is an interesting topic in materials chemistry and heterogeneous catalysis. This study reports a facile in situ method for the synthesis and immobilization of small silver nanoparticles (AgNPs) at room temperature on natural eggshell membrane (ESM), which presents interwoven fibrous structure and can be used as a unique protein-based biotemplate. Procyanidin (Pro), a typical plant polyphenol extracted from grape seeds and skins, was first grafted onto ESM fibers to serve as both reductant and stabilizer during the synthesis process. As a result, the AgNPs were facilely synthesized and robustly immobilized on the ESM fibers without additional chemical reductant or physical treatments. The morphology and microstructure of the as-prepared AgNPs@Pro-ESM composites were characterized by combined microscopy and spectroscopy technologies. The results indicate that small AgNPs with mean diameter of 2.46 nm were successfully prepared on the Pro-ESM biotemplate. The composites exhibited good catalytic activity toward the reduction of 4-nitrophenol (4-NP). More importantly, these composite catalysts can be easily recovered and reused for more than eight cycles because of their high stability.
研究领域:
材料化学
·
异相催化
·
纳米技术
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DOI
高影响因子
Journal of Translational Medicine
·
2024
·
IF 6.1
Wenbin Hua,Lin Xie,Chenpeng Dong,Guoyu Yang,Shouyuan Chi,Zhiqiang Xu,Cao Yang,Huiwen Wang,Xinghuo Wu
Intervertebral disc degeneration (IVDD) is a common cause of low back pain. Procyanidin C1 (PCC1) has been demonstrated to exert a protective effect on nucleus pulposus (NP) cells, and therefore, plays a critical role in the prevention and therapy of IVDD. Clarifying the pathophysiological characteristics and molecular mechanisms of IVDD may be helpful in establishing novel preventive and therapeutic strategies. This study aimed to investigate the probable mechanisms underlying the protection against acidic pH stress induced human NP cell injury. In vitro, acidic pH stress induced degeneration, mitochondrial dynamics imbalance, mitophagy, and mitochondria-mediated apoptosis in NP cells, all of which were ameliorated by PCC1. Autophagy inhibition partially eliminated the protective effects of PCC1 on mitochondrial homeostasis in NP cells. Moreover, PCC1 activated the sirtuin 3 (SIRT3)/forkhead box O3 (FOXO3) signaling pathway, a pivotal signaling pathway involved in the regulation of mitochondrial homeostasis in NP cells. In vivo, PCC1 ameliorated IVDD in a rat model and preserved the extracellular matrix of NP cells. Consequently, the protective effects of PCC1 on NP cells may inhibit IVDD progression via regulation of the SIRT3/FOXO3 signaling pathway. Therefore, regulation of the SIRT3/FOXO3 signaling pathway may be a novel preventive and therapeutic strategy for IVDD.
研究领域:
骨科学
·
分子生物学
·
细胞生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Changhong Li,Taotao Dai,Jun Chen,Xin Li,Ti Li,Chengmei Liu,David Julian McClements
The solution turbidity and intrinsic fluorescence quenching increased after procyanidin was mixed with lactoferrin. The addition of procyanidin also caused a reduction in the surface hydrophobicity of the lactoferrin, suggesting procyanidin bound to non-polar patches on lactoferrin’s surfaces. Moreover, the binding interaction caused an appreciable alteration in the structure of both the polyphenol and protein. Thermodynamic analysis indicated the interaction was spontaneous and mainly driven by entropy changes, suggesting that hydrophobic interactions dominated. A computational docking simulation provided insights into the location of the most-likely binding sites on the protein, as well as the nature of the interaction forces involved. In particular, both hydrophobic and hydrogen bonding were found to be important. The binding of the procyanidin to the lactoferrin enhanced its foaming properties. These results may lead to the development of a new class of natural functional ingredients that can be used in food products to improve their quality attributes.
研究领域:
食品科学
·
生物化学
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DOI
PubMed
高影响因子
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