LANGMUIR
·
2013
·
IF 4.187
Yunchao Long,Tao Wang,Lvdan Liu,Guangming Liu,Guangzhao Zhang
By use of a quartz crystal microbalance with dissipation (QCM-D), we have investigated the specific ion effect on the growth of poly(sodium 2-acrylamido-2-methylpropanesulfonate)/poly(diallyldimethylammonium chloride) multilayer at a salt concentration as low as 2.0 mM in water–methanol mixtures. QCM-D results demonstrate that specific ion effect can be observed in methanol and water–methanol mixtures though it is negligible in water. Moreover, the specific ion effect is amplified as the molar fraction of methanol (xM) increases from 0% to 75% but is weakened again with the further increase of xM from 75% to 100%. Nuclear magnetic resonance measurements reveal that the counterion–polyelectrolyte segment interactions may not account for the observed ion specificity. By extending the Collins’ concept of matching water affinities to methanol and water–methanol mixtures, we suggest that the ion–solvent interactions and the resulted counterion–charged group interactions are responsible for the occurrence of the specific ion effect. The conductivity measurements indicate that water and methanol molecules may form complexes, and the change of relative proportion of complexes with the xM causes the amplification or weakening of the specific ion effect.
研究领域:
材料科学
·
物理化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Water Reuse and Desalination
·
2018
·Vol. 9
·期 1
·18-30
·
IF 0.688
Wen Yue,Zhang Xingran,Chen Mei,Wu Zhichao,Wang Zhiwei
In this study, we systematically evaluated the antibiofouling behavior of quaternary ammonium compound (QAC) blended polyvinylidene fluoride (PVDF) membrane using quartz crystal microbalance with dissipation monitoring (QCM-D) combined with flow cytometry (FCM) and confocal laser scanning microscopy (CLSM) measurements. QCM-D tests showed that the introduction of QAC reduced bacterial attachment due to the biocidal functions of QAC. FCM indicated that cell integrity of the bacteria in the suspension flowing along QAC-modified membrane surfaces during the QCM-D test was severely affected. CLSM confirmed the significantly lower attachment of bacteria and higher dead/live cell ratio onto the surface of modified membranes after the washing step in QCM-D tests. Both FCM and CLSM results validated the antibacterial behavior of QAC-modified membranes by a contact-killing mechanism, which is in agreement with that of QCM-D tests. In addition, the bacterial cells accumulated on modified membrane surface exhibited higher reversibility compared to the control membrane, indicating ease of membrane cleaning. The results highlight that the combined use of QCM-D, FCM, and CLSM can comprehensively characterize the antibiofouling behavior of membranes.
研究领域:
材料科学
·
环境工程
·
膜技术
·
微生物学
·
表面化学
✓ 已验证
DOI
高影响因子
HOLZFORSCHUNG
·
2023
·Vol. 77
·期 6
·437-452
·
IF 2.4
Haonan Zhang,Qi Liu,Yanchen Zhu,Zhang Feng,Hao Ren,Huamin Zhai
In this study, representative lignin samples differing in hydroxyl content, molecular weight, linkage composition and lignin units ratio were prepared from eucalyptus wood by different isolation means and modification means: milled wood lignin (MWL), kraft lignin classified at different pH (KL), lignocresol (LC), lignoresorcinol (LR), lignopyrogallol (LP) as well as lignocresol secondary functional switched samples (LC 2 nd deriv). The structure of various lignins was characterized in detail by Fourier transform infrared (FT-IR) spectroscopy, gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). In order to eliminate the interference of other factors, bovine serum albumin (BSA) with relatively simple protein structure and different kinds of lignin and their derivatives were selected as the research objects. The adsorption/desorption behavior and mechanism between lignin samples and BSA were studied by quartz crystal microbalance with dissipation monitoring (QCM-D). The results showed that the content of phenolic hydroxyl was the main factor affecting the interaction of lignin and BSA, but in addition, the molecular size, linkages composition and the types of lignin units also had important effects on its adsorption behavior. The research will provide theoretical guidance and all-round reliable basic data for the interaction of lignin on lignocellulose hydrolysis enzymes in industrial application.
研究领域:
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Xuliang Lin,Xueqing Qiu,Duming Zhu,Zihao Li,Ningxin Zhan,Jieyi Zheng,Hongming Lou,Mingsong Zhou,Dongjie Yang
Effect of the molecular structure of lignin-based polyoxyethylene ether (EHL–PEG) on enzymatic hydrolysis of Avicel and corn stover was investigated. With the increase of PEG contents and molecular weight of EHL–PEG, glucose yield of corn stover increased. EHL–PEG enhanced enzymatic hydrolysis of corn stover significantly at buffer pH 4.8–5.5. Glucose yield of corn stover at 20% solid content increased from 32.8% to 63.8% by adding EHL–PEG, while that with PEG4600 was 54.2%. Effect of EHL–PEG on enzymatic hydrolysis kinetics of cellulose film was studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). An enhancing mechanism of EHL–PEG on enzymatic hydrolysis kinetics of cellulose was proposed. Cellulase aggregates dispersed by EHL–PEG excavated extensive cavities into the surface of cellulose film, making the film become more loose and exposed. After the maximum enzymatic hydrolysis rate, the film was mainly peeled off layer by layer until equilibrium.
研究领域:
生物质转化
·
酶工程
·
高分子化学
·
表面科学
·
可再生能源
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2026
·
IF 4.4
Yong Wang,Chunxiao Yan,Weiye Liang,Qiang Zhang,Gang Cheng,Jicheng Dong,Lin Jin,Huan Tang,Fang Cheng,Lijun Zhang
Orthokeratology (OK) lenses for myopia correction are susceptible to biofouling by tear-derived biomolecules, escalating the risks of ocular infection and inflammation. Prevailing studies that rely on end point protein quantification fail to capture the real-time kinetics of fouling formation. Here, we fabricated a UV pressure-assisted polymer-grafted quartz crystal microbalance with dissipation monitoring (QCM-D) sensor exhibiting exceptional stability, nanoscale smoothness (RMS roughness ≈2 nm), and interfacial peel resistance. This platform enables in situ tracking of adsorption/desorption kinetics for four critical tear components: native/denatured lysozyme and oxidized/native lecithin. Key findings reveal a flow-dependent fouling behavior, wherein low flow rates increase biomolecule adsorption by 37–80% compared with higher flows. We further identify denatured lysozyme and native lecithin as resilient contaminants characterized by a stronger deposition affinity and pronounced resistance to elution. Quantitative screening of multipurpose solutions (MPSs) demonstrates that MPS #2 achieves 20–100% elution rate across biomolecules, outperforming commercial benchmarks. By leveraging dissipation–frequency (D–F) analysis, we clarify the fundamental mechanisms of biofouling formation at the molecular level. Collectively, this work establishes three critical advances: (1) a real-time biofouling diagnostic platform for OK lens interfaces, (2) molecular design principles for antifouling materials based on adhesion remodeling theory, and (3) an accelerated MPS formulation screening paradigm for ocular device safety.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MINERALS ENGINEERING
·
2025
·
IF 5
Ting Xiao,Wenjie Zou,Congzhong Tian,Wentao Hu,Zhijun Zhang,Chengyan Xu,Fang Zhou
Green and efficient flocculants are crucial for the effective treatment and recycling of mine wastewater. Herein, the flocculation performance and mechanism of a novel amphoteric chitosan-based flocculant CMC-g-CPAM on kaolinite suspensions were investigated through settling tests, zeta potential measurements, floc size distribution, quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM) measurements. Settling tests results revealed that CMC-g-CPAM displayed exceptional flocculation performance, achieving a settling rate of 18.21 m/h for kaolinite at natural pH, higher than that of chitosan-grafted-cationic acrylamide Chi-g-CPAM (11.19 m/h). The in-situ adsorption layer of CMC-g-CPAM on silica and alumina surfaces was more rigid than that of Chi-g-CPAM. Subsequently, more kaolinite particles were flocculated by CMC-g-CPAM, and formed a denser floc layer. The adsorption amounts of CMC-g-CPAM and Chi-g-CPAM on silica surfaces were found to be greater than those observed on alumina surfaces at natural pH. This phenomenon was related to the different surface charges of silica and alumina, electrostatic interactions between the polymers and surfaces. From AFM measurements, an attraction between the CMC-g-CPAM functionalized probe and the functional substrate as they approached each other and a weak adhesion force among CMC-g-CPAM (0.31 nN over 50 nm) were observed. These indicated the presence of intermolecular interactions among CMC-g-CPAM, which contributed to the formation of compact flocs. In contrast, an obvious repulsion was detected when the Chi-g-CPAM functionalized probe approached the functional substrate, inhibited the entanglement of polymer chains. Meanwhile, the interactions between multiple molecules and the substrate displayed a large adhesion force (2.10 nN over 150 nm), attributed to the less rigid adsorption layer of Chi-g-CPAM. Our findings would provide new insights into the design of flocculants and effective wastewater treatment.
研究领域:
环境工程
·
高分子科学
·
胶体与界面科学
·
矿物加工
·
废水处理
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Yibang Zhang,Dong Chen,Yang Liu,Aisha Labaran Ammani,Atena Ahmadian,Aftab Ullah
Protein-lipid interactions are pivotal in various biological processes, including membrane trafficking, signal transduction, and cellular compartmentalization. This study investigates how fatty acid modification alters the physicochemical properties of ovalbumin (OVA) and its interactions with lipid bilayers, with implications for controlled release applications. We hypothesize that modifying OVA with different fatty acids will significantly alter its surface hydrophobicity and membrane affinity, thereby influencing its potential for targeted drug delivery. OVA was chemically modified with four fatty acids (myristic, palmitic, stearic, and oleic acids) using N-hydroxysuccinimide (NHS) esters. Surface hydrophobicity was assessed via 8-Anilino-1-naphthalenesulfonic acid ammonium (ANS) fluorescence assays, revealing that modifications increased hydrophobicity, particularly with longer fatty acid chains. Interactions with liposomes were characterized using dynamic light scattering (DLS) and zeta potential measurements, indicating enhanced liposome binding and altered bilayer properties due to fatty acid modifications. Quartz crystal microbalance with dissipation (QCM-D) analysis confirmed the insertion of fatty acid chains into the lipid bilayer. In vitro release experiments demonstrated that fatty acid modification significantly affected the release kinetics of OVA, with oleic acid-modified OVA showing the lowest cumulative release. Our findings highlight the role of fatty acid structure in modulating protein-lipid interactions and underscore the potential of fatty acid-modified OVA for developing targeted drug delivery systems. Future work will explore the underlying mechanisms and evaluate the therapeutic potential of these modified proteins in vivo.
研究领域:
生物物理学
·
生物化学
·
药学
·
分子生物学
·
胶体与界面科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Qian Wang,Juan Li,Qiuyueming Zhou,Ting He,Zhaoliang Cui,Young Moo Lee,Weihong Xing
Dissolved organic matter (DOM) is recognized as a crucial factor contributing to the irreversible fouling of ultrafiltration membranes. Quartz crystal microbalance with dissipation (QCM-D) offers insights into the kinetics of fouling layer formation and its structure in-situ on a model membrane. However, a clear correlation between the membrane fouling process and the adsorption-desorption results obtained from QCM-D has yet to be established. In this study, piezoelectric β-phase PVDF membranes were prepared, and their fouling behavior in response to three model compounds (humic acid (HA), dextran (DEX), bovine serum albumin (BSA), and their mixed solutions was examined. It was firstly proposed that the spin-coated model PVDF membrane surface exhibited a typical β-phase structure, characterized by regular undulations resulting from strong stretching effects. A novel double layer piezoelectric sensor, consisting of a β-phase layer over a piezoelectric quartz substrate, was designed for QCM-D analysis to assess the mass and stiffness variations of the absorbed DOM layer. The fouling tendency followed the order of BSA > Mixture > DEX > HA, with the foulant monomeric units having a similar molecular mass of around tens of kDa. Irreversible fouling resistance in series model as well as frequency drop in QCM-D were listed as indicators of irreversible fouling caused by foulant-membrane interaction, which realized a well-defined match for the first time.
研究领域:
膜科学
·
环境工程
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
npj Science of Food
·
2022
·Vol. 6
·期 1
·1-8
·
IF 7
Chong Pik Han,Chen Jianshe,Yin Danting,Qin Lanxi
Huigan is an important sensory attribute which is commonly used as a quality indicator evaluation of tea products. Previous studies showed a strong correlation between the lubrication behavior of saliva-tea compound mixture and the sensory perception of Huigan from trained panelists. This work was further designed to investigate how the effect of tea consumption on the rate of saliva secretion and its functional properties including total protein content of saliva (TPC), salivary α-amylase (AMY) and lipase activity (LP). A quartz crystal microbalance with dissipation monitoring (QCM-D) was applied to reveal the adsorption behavior of human whole saliva and how the salivary film is affected by the presence of tea compounds. Results showed a significant positive correlation among TPC, LP and Huigan intensity for subjects who are Huigan-sensitive. Compared to the desorption of salivary film, the desorption of saliva-EC/EGC (epicatechin/epigallocatechin) mixture from the gold surface by QCM-D observation showed a significant effect on Huigan intensity in sensitive group when comparing to the salivary layer (blank).
研究领域:
食品科学
·
感官评价
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yueling Tian,Manyan Qiu,Yu Shen,Yaping Zheng,Xinyan Yang,Wei Zhang,Yujun Jiang
Whey protein hydrolysate (WPH) can be used to develop hypoallergenic foods. However, the stabilization mechanism of WPH-stabilized emulsion is not fully understood. Here, a real-time quartz crystal microbalance with dissipation monitoring (QCM-D) was used in conjunction with a rheometer to investigate the interfacial properties of WPH. Initially, the properties of WPH with different (6 %, 8 %, 10 %, 12 % and 14 %) degree of hydrolysis (DH) were investigated. 8 %-WPH demonstrated superior emulsifying (11.49 m 2 /g, 81.34 min) and foaming properties (14.00 %, 7.78 %). Subsequently, the stability of different WPH-stabilized emulsions were examined. 8 %-WPH emulsion exhibited the lowest centrifugal precipitation rate (4.50 %) and Turbiscan stability index (2.24). Additionally, the 8 %-WPH promoted the adsorption and retention of molecules at the interface, which effectively reduced the interfacial tension. QCM-D measurement further proved that the 8 %-WPH possessed excellent adsorption mass and viscoelasticity. Finally, we characterized the interface-adsorbed WPH. The 8 %-WPH exhibited the highest surface hydrophobicity (1072.60) and flexibility (0.22). Notably, the 8 %-WPH showed the highest β-sheet (41.11 %). This led to stronger interactions between neighboring interfacial WPH molecules, which protected the emulsion droplets from destabilizing factors. Nevertheless, excessive hydrolysis (10 %–14 %) caused WPH molecules aggregation, which consequently diminished the viscoelasticity of the interfacial film and the emulsion stability.
研究领域:
食品科学
·
胶体与界面化学
·
生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2013
·
IF 4.187
Tao Wang,Xiaowen Wang,Yunchao Long,Guangming Liu,Guangzhao Zhang
The conformation of polyzwitterionic brushes plays a crucial role in the adsorption/desorption of proteins on solid surfaces. By use of quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR), we have systematically investigated the conformational behavior of poly(sulfobetaine methacrylate) (PSBMA) brushes as a function of ionic strength in the presence of different ions. The frequency change demonstrates that the effectiveness of anions to weaken the inter/intrachain association and to enhance the hydration of the grafted chains increases from kosmotrope to chaotrope in the low ionic strength regime, but the ordering of anions is almost reversed at the high ionic strengths. The dissipation change indicates that some heterogeneous structures are formed inside the brushes in the presence of chaotropic anions with the increase of ionic strength. In SPR studies, the change of resonance unit (ΔRU) with ionic strength is determined by the balance between the increase of thickness and the decrease of refractive index of the brushes. No anion specificity is observed in the SPR measurements because ΔRU is insensitive to the coupled water molecules inside the brushes. For the control of protein adsorption/desorption, our studies show that the brushes can more effectively resist the protein adsorption in the presence of a more chaotropic anion and a more chaotropic anion can also more effectively induce the protein desorption from the surface of the brushes. In addition, no obvious cation specificity can be observed in the conformational change of the brushes in either QCM-D or SPR measurements.
研究领域:
材料科学
·
生物物理学
·
表面化学
·
高分子科学
✓ 已验证
DOI
PubMed
高影响因子
WOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 56
·期 1
·305-322
·
IF 2.898
Li Mingfu,Liu Yi,Chen Chunlin,Zhang Jian,Wang Shuangfei,Min Douyong
The hot water and green liquor pretreatments were conducted on Eucalyptus wood in the current study. The cellulase adsorption behavior on the isolated lignins and model compounds was clarified by a quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM). The QCM-D analysis indicated that the hot water pretreated lignin (HPL) adsorbed more cellulase than the green liquor pretreated lignin (GPL) and the protolignin (PL). The AFM analysis revealed that the interaction force between lignin and cellulase was in the order of HPL > GPL > PL which was consistent with the cellulase adsorption. The monomeric lignin model with the ketone group adsorbed more cellulase than the counterpart with the hydroxyl group or aldehyde group. The dimeric lignin models adsorbed more cellulase than the monomeric lignin models. Furthermore, the β – β dimer model adsorbed more cellulase than the β–5 and 5–5′ dimer models. This study provides fundamental knowledge for developing more efficient pretreatment technologies.
研究领域:
生物化学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）