Scientific Reports
·
2016
·Vol. 6
·期 1
·1-13
·
IF 5.228
Raza Waseem,Ling Ning,Yang Liudong,Huang Qiwei,Shen Qirong
It is important to study the response of plant pathogens to the antibiosis traits of biocontrol microbes to design the efficient biocontrol strategies. In this study, we evaluated the role of volatile organic compounds (VOCs) produced by a biocontrol strain Bacillus amyloliquefaciens SQR-9 on the growth and virulence traits of tomato wilt pathogen Ralstonia solanacearum (RS). The VOCs of SQR-9 significantly inhibited the growth of RS on agar medium and in soil. In addition, the VOCs significantly inhibited the motility traits, production of antioxidant enzymes and exopolysaccharides, biofilm formation and tomato root colonization by RS. The strain SQR-9 produced 22 VOCs, but only nine VOCs showed 1–11% antibacterial activity against RS in their corresponding amounts; however, the consortium of all VOCs showed 70% growth inhibition of RS. The proteomics analysis showed that the VOCs of SQR-9 downregulated RS proteins related to the antioxidant activity, virulence, carbohydrate and amino acid metabolism, protein folding and translation, while the proteins involved in the ABC transporter system, amino acid synthesis, detoxification of aldehydes and ketones, methylation, protein translation and folding, and energy transfer were upregulated. This study describes the significance and effectiveness of VOCs produced by a biocontrol strain against tomato wilt pathogen.
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DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-15
·
IF 4.259
Tahir Hafiz Abdul Samad,Gu Qin,Wu Huijun,Niu Yuedi,Huo Rong,Gao Xuewen
Volatile organic compounds (VOCs) produced by various bacteria have significant potential to enhance plant growth and to control phytopathogens. Six of the most effective antagonistic Bacillus spp. were used in this study against Ralstonia solanacearum (Rsc) TBBS1, the causal agent of bacterial wilt disease in tobacco. Bacillus amyloliquefaciens FZB42 and Bacillus artrophaeus LSSC22 had the strongest inhibitory effect against Rsc . Thirteen VOCs produced by FZB42 and 10 by LSSC22 were identified using gas chromatography-mass spectrometry analysis. Benzaldehyde, 1,2-benzisothiazol-3(2 H)-one and 1,3-butadiene significantly inhibited the colony size, cell viability, and motility of pathogens and negatively influenced chemotaxis. Transmission and scanning electron microscopy revealed severe morphological and ultra-structural changes in cells of Rsc. Furthermore, VOCs altered the transcriptional expression level of PhcA (a global virulence regulator), type III secretion system (T3SS), type IV secretion system (T4SS), extracellular polysaccharides and chemotaxis-related genes, which are major contributors to pathogenicity, resulting in decreased wilt disease. The VOCs significantly up-regulated the expression of genes related to wilt resistance and pathogen defense. Over-expression of EDS1 and NPR1 suggest the involvement of SA pathway in induction of systemic resistance. Our findings provide new insights regarding the potential of antibacterial VOCs as a biocontrol tool against bacterial wilt diseases.
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2016
·Vol. 100
·期 17
·7639-7650
·
IF 3.376
Raza Waseem,Wang Jichen,Wu Yuncheng,Ling Ning,Wei Zhong,Huang Qiwei,Shen Qirong
The production of volatile organic compounds (VOCs) by microbes is an important characteristic for their selection as biocontrol agents against plant pathogens. In this study, we identified the VOCs produced by the biocontrol strain Bacillus amyloliquefaciens T-5 and evaluated their impact on the growth and virulence traits of tomato bacterial wilt pathogen Ralstonia solanacearum. The results showed that the VOCs of strain T-5 significantly inhibited the growth of R. solanacearum in agar medium and in soil. In addition, VOCs significantly inhibited the motility traits, root colonization, biofilm formation, and production of antioxidant enzymes and exopolysaccharides by R. solanacearum . However, no effect of VOCs on the production of hydrolytic enzymes by R. solanacearum was observed. The strain T-5 produced VOCs, including benzenes, ketones, aldehydes, alkanes, acids, and one furan and naphthalene compound; among those, 13 VOCs showed 1–10 % antibacterial activity against R. solanacearum in their produced amounts by T-5; however, the consortium of all VOCs produced on agar medium, in sterilized soil, and in natural soil showed 75, 62, and 85 % growth inhibition of R. solanacearum , respectively. The real-time PCR analysis further confirmed the results when the expression of different virulence- and metabolism-related genes in R. solanacearum cells was decreased after exposure to the VOCs of strain T-5. The results of this study clearly revealed the significance of VOCs in the control of plant pathogens. This information would help to better comprehend the microbial interactions mediated by VOCs in nature and to develop safer strategies to control plant disease.
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Yao Wang,Yijia Liang,Xingjun Ma,Yanling Dong,Matthana Klakong,Anbin Wang,Mengle Chen,Tianyu Fan,Xiaohong Xu,Peng Xiao,Wei Ding
Frequent or excessive use of synthetic pesticides can easily lead to pollution of soil and groundwater in management of soil-borne diseases. An environmental-friendly vanadium carbide MXene nanosheets (V 2 C-NSs) have been widely studied in the field of nanomedicine and biomaterials. However, few studies have shown has direct effects of V 2 C-NSs on crop protection and induces plant resistance to disease. In this study, V 2 C-NSs were first prepared by artificial synthesis and characterized, and the resulting material displayed an excellent Tyndall effect. The ability of V 2 C-NSs to promote plant growth and biosafety and its molecular mechanism of inducing plant resistance to Ralstonia solanacearum ( R. solanacearum ) were investigated. The results showed that V 2 C-NSs could increase plant fresh (or dry) weight, promote ROS accumulation, activate plant defense enzymes, and upregulate ethylene (ET) and jasmonic acid (JA) synthesis as well as the expression of ET/JA-responsive genes. Interestingly, the MAPK signaling pathway, biosynthesis of henylpropanoid and flavonoid were also activated, but the salicylic acid biosynthetic pathway was deactivated. Thus, V 2 C-NSs likely induced plant resistance to R. solanacearum via the ET/JA pathway. Furthermore, foliar application of V 2 C-NSs could delay occurrence of tobacco bacterial wilt, while the control efficiency reached 65.52 % nine days post-inoculation (dpi), followed by 57.00 % and 52.99 % at 11 dpi and 13 dpi, respectively, which were significantly greater than the control benzothiadiazole (BTH). Moreover, the biosafety of V 2 C-NSs was evaluated using tobacco seeds (Yunyan87), bacteria and earthworms. This study suggests that V 2 C-NSs can potentially be used as an environmental-friendly plant resistance inducer for controlling bacterial wilt diseases in sustainable crop protection.
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